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[Display omitted] • A novel dual-excitation ratiometric fluorescent probe for the specific detection of ONOO– was designed and synthesized. • Probe 1 showed fast response toward ONOO– with high selectivity and sensitivity. • Probe 1 can specifically image endogenous and exogenous ONOO– in living cells and zebrafish. Peroxynitrite (ONOO−) is a highly reactive oxygen species that plays a critical role in many physiological and pathological processes of cell function. This study aimed to propose a ratiometric fluorescent probe BDHCA derived from coumarin for determining the ONOO− level. ONOO− could specifically induce oxidative cleavage of the conjugated C = C double bond in probe BDHCA , providing a fluorescent ratiometric output. The response of probe BDHCA to ONOO− was selective, fast, and highly sensitive, with a detection limit of 50.3 nM. Biological imaging experiments suggested that probe BDHCA could be used to image ONOO− in living RAW264.7 cells and zebrafish. [ABSTRACT FROM AUTHOR]

Herein, a novel ternary composite material has been proposed for the electrochemical detection of carbendazim (CBZ) pesticide. Such Au nanoparticles@zeolitic imidazole framework-L-Ti 3 C 2 T x (AuNPs@ZIF-L-Ti 3 C 2 T x) can be obtained by using few-layer Ti 3 C 2 T x and AuNPs@ZnO as the precursors. The original synthetic route leads to the distinguished morphology of AuNPs@ZIF-L-Ti 3 C 2 T x , which exhibits the salix leaf-like morphology that is accompanied by two kinds of topological structures including the dart-like and carambola-like shapes. More importantly, the unique structure of AuNPs@ZIF-L-Ti 3 C 2 T x contributes to high-performance sensing for the determination of CBZ. A series of control experiments has been performed in order to investigate the structure-activity relationship and sensing mechanism of AuNPs@ZIF-L-Ti 3 C 2 T x. According to the material characterization results and performance testing results, the synergistic effect of AuNPs, ZIF-L and Ti 3 C 2 T x is considered as the key of high-efficiency reaction interface that leads to the electrocatalytic oxidation of CBZ. The established electrochemical sensor based on the non-enzymatic AuNPs@ZIF-L-Ti 3 C 2 T x shows a low limit of detection is 1.2 nM. Notably, the highly sensitive determination of CBZ can be realized by AuNPs@ZIF-L-Ti 3 C 2 T x , owing to 66,684 μA mM−1 cm−2 of sensitivity. The two-stage linear detection ranges are 0.01–0.20 μM and 0.20–20.00 μM. The potential application of AuNPs@ZIF-L-Ti 3 C 2 T x has been displayed to develop high-performance sensor. [Display omitted] • The novel Au nanoparticles@zeolitic imidazole framework-L-Ti 3 C 2 T x is proposed. • The salix leaf-like morphology accompanies by two kinds of topological structures. • Synergistic ternary composites contribute high-performance electrochemical sensor. • AuNPs@ZIF-L-Ti 3 C 2 T x has potential application for nonenzymatic carbendazim sensing. • The detection limit is 1.2 nM; the sensitivity is 66,684 μA mM−1 cm−2. [ABSTRACT FROM AUTHOR]