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[Display omitted] • Design, synthesis and characterization of multiple helix. • Construction of helical foldamers-based hybrid channel with high ion transport activities. • Proof of the good translocation properties of helix with high capture rate. • Good sensitivity in the detection of unimolecular amino acids and enantiomers with hybrid nanochannels. Nanopore platform has been explored for wide applications in various domain. However, the intrinsic properties of the pore-carrier materials limit the scaled practical usage. Some hybrid systems combined the advantages of each material have been promoted, most of which are based on the fragile lipid bilayer-supported transmembrane proteins or DNA origami on the solid-state channel. We herein provide a lipid-free construction of hybrid nanochannel equipped with helical molecule embedding in SiN x nanopore. The mechanical robustness and the physiochemical stability of SiN x and designable biocompatible helix supply a superior integrated device for stable durable sensing with good reliability. The hybrid nanochannels display a good conduction in distinct ionic solutions with both hydrophilic and hydrophobic multiplex helix. The identification of different amino acids as well as the enantiomers reveal the potential capability of the hybrid nanochannel in the peptide and protein sensing at single amino acid level. Our work paves a way on the solid-state nanopore sequencing with sub-nanometer-sized organic/inorganic hybrid ionic channel. [ABSTRACT FROM AUTHOR]

• Electrochemical, nanopore and field-effect transistors biosensor has been developed. • Aptamer, DNAzyme, DNA origami and nanomaterials has been used for amplification. • The biosensor exhibits an excellent performance for amplification detection. • We discussed the challenges of detection strategies in clinical application. Due to the wide range of electrochemical devices available, DNA nanostructures and material-based technologies have been greatly broadened. They have been actively used to create a variety of beautiful nanostructures owing to their unmatched programmability. Currently, a variety of electrochemical devices have been used for rapid sensing of biomolecules and other diagnostic applications. Here, we provide a brief overview of recent advances in DNA-based biomolecular assays. Biosensing platform such as electrochemical biosensor, nanopore biosensor, and field-effect transistor biosensors (FET), which are equipped with aptamer, DNA walker, DNAzyme, DNA origami, and nanomaterials, has been developed for amplification detection. Under the optimal conditions, the proposed biosensor has good amplification detection performance. Further, we discussed the challenges of detection strategies in clinical applications and offered the prospect of this field. [ABSTRACT FROM AUTHOR]