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• A D-p-A type probe has been developed for detection of ClO−. • The mechanism associated with oxidation breakage of rhodanine moiety was proposed. • The crystal structure of the probe is reported. • The probe has been successfully applied to the imaging of ClO− in cells, plants and zebrafish. Hypochlorite (ClO−) plays an important role in many cellular processes, which is proved to be related to some serious diseases. Given the electron donating and electron withdrawing properties of benzo[ h ]chromene and rhodanine groups, respectively, we developed a Donor (D)-p-Acceptor (A) type probe 1 for the detection of ClO− based on benzo[ h ]chromene (D) and rhodanine (A), whose structure was determined by ESI-MS, NMR and X-ray diffraction. Probe 1 showed a fast (within 20 s), selective and sensitive response toward ClO− via a fluorescent turn-on output. Based-on the spectral change of probe 1 induced by ClO−, the mechanism associated with oxidation breakage of rhodanine moiety was proposed. Due to the excellent biocompatibility and low cytotoxicity, probe 1 can be used for imaging of ClO− in C6 cells, Arabidopsis thaliana, and zebrafish. [Display omitted] [ABSTRACT FROM AUTHOR]

[Display omitted] • A novel dual-excitation ratiometric fluorescent probe for the specific detection of ONOO– was designed and synthesized. • Probe 1 showed fast response toward ONOO– with high selectivity and sensitivity. • Probe 1 can specifically image endogenous and exogenous ONOO– in living cells and zebrafish. Peroxynitrite (ONOO−) is a highly reactive oxygen species that plays a critical role in many physiological and pathological processes of cell function. This study aimed to propose a ratiometric fluorescent probe BDHCA derived from coumarin for determining the ONOO− level. ONOO− could specifically induce oxidative cleavage of the conjugated C = C double bond in probe BDHCA , providing a fluorescent ratiometric output. The response of probe BDHCA to ONOO− was selective, fast, and highly sensitive, with a detection limit of 50.3 nM. Biological imaging experiments suggested that probe BDHCA could be used to image ONOO− in living RAW264.7 cells and zebrafish. [ABSTRACT FROM AUTHOR]