1. An alkaloid-rich phytopharmaceutical prepared from Qing Dai against IL-17A-induced psoriasis.
- Author
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Lee, Chia-Lin, Wang, Chien-Ming, Song, Ying-Chyi, Liu, Chuan-Teng, Chu, Mei-Yun, and Yen, Hung-Rong
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SKIN disease prevention , *INFLAMMATION prevention , *PSORIASIS , *INTERLEUKINS , *IN vitro studies , *QUINOLINE , *DRUG efficacy , *HERBAL medicine , *IN vivo studies , *HIGH performance liquid chromatography , *SPECTROPHOTOMETERS , *SKIN , *ALKALOIDS , *ANIMAL experimentation , *CHEMICAL reagents , *GENE expression , *OXIDATIVE stress , *SEVERITY of illness index , *QUALITY of life , *DRUG development , *PLANT extracts , *CHROMATOGRAPHIC analysis , *CHINESE medicine , *MICE , *KERATINOCYTES ,THERAPEUTIC use of alkaloids - Abstract
Psoriasis is a chronic inflammatory disease due to immune dysregulation that cannot be cured. The skin conditions of psoriasis negatively impact patients' quality of life worldwide. Qing Dai (Indigo Naturalis), a traditional Chinese medicine (TCM) processed from Strobilanthes cusia is a clinical medicine used for psoriasis patient in Taiwan and the gene overexpression of interleukin (IL)-17A could be notably reduced in skin lesions after using Qing Dai ointment and its alkaloid ingredients. To develop a potential anti-psoriatic phytopharmaceutical, an alkaloid-rich fraction named INM-A was prepared from Qing Dai. The chemical profile and anti-psoriatic activity of INM-A were analyzed and evaluated to define its in vitro mechanism and in vivo efficacy for psoriasis therapy. Dowex® 50WX4 hydrogen form resin was used for column chromatography to prepare INM-A. To track alkaloids, INM-A was conducted with Dragendorff's, Mayer's, and Wagner's reagents. HPLC and UV–Vis spectrophotometer were applied to analyze the chemical profile and relative total alkaloid content in INM-A. A psoriatic mouse model induced by imiquimod (IMQ) was performed to verify in vivo efficacy of INM-A. IL-17A-dominated cellular oxygen consumption rate, oxidative stress, and cytokines in keratinocytes were measured to clarify in vitro mechanism of INM-A. An alkaloid-rich fraction, INM-A, consisted of seven active alkaloid compounds 1 – 7 was obtained from Qing Dai. INM-A improved the skin condition severities in IMQ-induced psoriatic mice and decreased IL-17A in not only psoriatic mice but also polarized Th17 cells. In addition, INM-A targeted IL-17A to inhibit inflammation and OXPHOS-driven oxidative stress in human keratinocytes. Accordingly, INM-A manufactured from Qing Dai may be a promising lead phytopharmaceutical for further IL-17A-related inflammatory disease studies such as psoriasis. [Display omitted] • An alkaloid-rich phytopharmaceutical (INM-A) was prepared from Qing Dai. • INM-A could alleviate the severities of dermatitis in IMQ-induced psoriatic mice. • INM-A could decrease IL-17A productions in psoriatic mice and polarized Th17 cells. • INM-A targeted IL-17A to inhibit inflammation (IL-6 and IL-8) in HaCaT cells. • INM-A targeted IL-17A to inhibit OXPHOS-driven oxidative stress in HaCaT cells. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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