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Background: In Italy, malaria was endemic until the 1970s, when it was declared eradicated by WHO. Nowadays, with the persistence of competent mosquito populations, the effect of climate change, and increased possibility of importing malaria parasites from endemic counties due to growing migration, a malaria resurgence in Italy has become more likely. Hence, enhancing the understanding of the current distribution of the Anopheles maculipennis complex and the factors that influence the presence of this malaria vector is crucial, especially in Northern Italy, characterised by a high density of both human population and livestock. Methods: To assess the presence and abundance of malaria vectors, a 4-year field survey in the plain areas of Lombardy and Emilia-Romagna region in Italy was conducted. Every sampling point was characterised in space by the land use in a 500-m radius and in time considering meteorological data collected in the short and long time periods before sampling. We combined the results of a linear regression model with a random forest analysis to understand the relative importance of the investigated niche dimensions in determining Anopheles mosquito presence and abundance. Results: The estimated normalised variable importance indicates that rice fields were the most important land use class explaining the presence of Anopheles, followed by transitional woodlands and shrubland. Farm buildings were the third variable in terms of importance, likely because of the presence of animal shelters, followed by urbanised land. The two most important meteorological variables influencing the abundance of Anopheles in our study area were mean temperature in the 24 h before the sampling date and the sum of degree-days with temperature between 18 °C and 30 °C in the 14 days before the sampling date. Conclusions: The results obtained in this study could be helpful in predicting the risk of autochthonous malaria transmission, based on local information on land cover classes that might facilitate the presence of malaria vectors and presence of short- and medium-term meteorological conditions favourable to mosquito development and activity. The results can support the design of vector control measures through environmental management. [ABSTRACT FROM AUTHOR]

• DOM molecular compositions in multi-interface runoffs were quantified by FT-ICR MS. • Soil-epikarst structures controlled the DOM quantity and molecular compositions. • High microbially-derived carbon was detected in epikarst flow. • Flow rate and discharge effected DOM quantity and quality during runoff process. • Soil thicknesses affected runoff DOM dynamics by impacting hydrological processes. Understanding the chemical composition, origin, and molecular structure of dissolved organic matter (DOM) in multi-interface runoff is essential for comprehending the fate of laterally transported DOM in complex soil-epikarst systems of karst hillslopes. Limited information, however, is available for the optical properties and molecular compositions of the transported OM in multiple runoff components on the karst hillslope in relation to land-uses and soil thicknesses. In this study, we conducted a study to observe the changes in the quantity and quality of DOM in multiple interface flow (surface, subsurface, and epikarst) during natural rainfall events in 2022 in karst hillslopes that are covered by different land uses (cropland and shrubland) and soil thicknesses (with mean depths of 66.0 cm for deeper soil and 35.4 cm for shallower soil) in the karst region of southwest China. chemcial compositions of runoff DOM were determined by optical analysis and microbial compositions in runoff were inferred with high-throughput sequencing. The results showed that the soil-epikarst structure was controlling the runoff DOM quantity and quality during rainfall events. A decrease in the aromaticity, humification, unsaturation, and oxidation degree and an increase in carbohydrate, aminosugars, protein, and lipid compounds were found from surface to epikarst flow, indicating that plant-and soil-derived carbon decreased, while the microbially-derived carbon increased. The results were further comfirmed by the higher bacterial richness and diversity, along with fungal diversity in the epikarst flow compared to other runoff components. The bio-labile protein materials (C2) were the most important component of runoff DOM output in karst hillslopes. In surface and subsurface flow, rainfall amount, runoff rate, and discharge significantly affected the DOM concentration and quality during rainfalls, indicating that the dynamics of DOM in runoff from karst hillslopes were predominantly influenced by hydrological processes. Furthermore, the runoff DOM quality in cropland was dominated by lower unsaturation and oxidation degrees and higher protein component, compared to those in shrubland. The compositions of DOM in runoff from hillslope plots with thicker soils were primarily characterized by microbially-derived materials. Our findings were conducive to understanding the mechanism governing the migration of DOM quality and quantity in discharge during multi-interface hydrological processes on karst hillslopes. [ABSTRACT FROM AUTHOR]

• Spatiotemporal DSL variation and associated forcings were explored at a semi-humid catchment. • Land use type was the dominant control on DSL variation at a catchment scale. • DSL response to meteorological factors differed between the semiarid and semi-humid catchments. • The mixed "forest/shrubland + grass" plantation type is recommended for the semi-humid catchment. The formation of dried soil layers (DSLs) is a broad consequence of climate change (i.e., through temperature increases and/or precipitation decreases) and poor land management practices, where these layers exist has severe negative effects (i.e., retardation of vegetation, negative water cycle balance) on the sustainability of restored ecosystems and health of agriculture. Because of the effort and time required, only sporadic in situ measurements on spatial and temporal DSL variation have been reported, particularly at a catchment scale. However, this information is essential for understanding mechanisms associated with evolutionary DSL processes as well as developing effective land management techniques. Accordingly, this study monitored soil water content to a 5 m depth over a 5-year period in a semi-humid catchment on the Loess Plateau while also investigating temporal DSL dynamics and dominant controls. In the semi-humid catchment, DSLs occurred at 90.7 % of all sampling sites. Spatially and temporally averaged DSL formation depth (DSLFD), DSL thickness (DSLT), and DSL soil water content (DSL-SWC) within the 0–5 m profile were 170 cm, 262 cm, and 9.52 %, respectively. Forest and shrubland DSL regimes were drier than those of grassland. These three DSL indices exhibited significant correlation with temperature variables, while only DSLFD variables significantly correlated to precipitation. Land use type was the predominant control of DSL variation at a catchment scale. A spatial DSLT variation comparison under different land use treatment positions determined the feasibility of planting grass at an upslope position and mixed forest/shrubland + grass at mid-slope and downslope positions in the semi-humid catchment, which provides a good case study for afforestation practices at a catchment scale. Findings from this study are intended to aid in catchment soil and water conservation, soil drought mitigation, and sustainable vegetation management practices on the Loess Plateau as well as other water-limited regions worldwide. [ABSTRACT FROM AUTHOR]