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An abrupt decrease in egg production and quality in laying hens can be brought on by the avian adenovirus known as the egg drop syndrome virus (EDSV). So far, there are no efficient commercial diagnostic approaches for EDSV. In the present study, we first purified two monoclonal antibodies (mAbs 5G4 and 6G6) specifically against the EDSV fibre protein. The mAb 5G4 was then employed as capture antibody. Additionally, the detection antibody 6G6 (HRP-6G6) was conjugated with horseradish peroxidase. Consequently, based on these two mAbs, we developed a sandwich enzyme-linked immunosorbent assay (ELISA) for EDSV detection. Specificity evaluation demonstrated that only EDSV gave a positive result while other avian viruses showed negative results. Sensitivity investigation showed that the limit of detection of EDSV was 102.9 TCID50/ml and the limit of detection of the purified His-Fiber was 5 ng/ml. Furthermore, the screening of samples from infected chickens showed that this method was very effective and could be applied to the detection of EDSV in infected samples. In summary, the sandwich enzyme-linked immunosorbent assay developed here provides an efficient, sensitive, and large-scale method for the specific diagnosis of EDSV. A sandwich ELISA was developed to detect EDSV using the mAbs 5G4 and HRP-6G6. The sandwich ELISA maintained high specificity and sensitivity. The sandwich ELISA had equivalent consistency with real-time PCR assay. [ABSTRACT FROM AUTHOR]

Wheat gliadin is thought to be the main cause of wheat allergy leading to celiac disease. In this study, we reported an electrochemical immunosensor based on AuNPs/Zn/Ni-ZIF-8-800@graphene for simple, efficient, and sensitive detection of gliadin. A monoclonal antibody against gliadin were prepared by hybridoma technique. Zn/Ni-ZIF-8-800 with high stability was prepared by using zinc–nickel bimetallic organic framework MOFs, and graphene and gold nanoparticles with large surface area and excellent conductivity were introduced. Chitosan was used as a binder to fixed the composite on the surface of glassy carbon electrode. The fabricated materials and the corresponding sensors were comprehensively characterized by X-ray diffractometer, scanning electron microscope, transmission electron microscopy, Fourier transform infrared spectroscopy, Brunauer–Emmett–Teller, and cyclic voltammetry analyses. Under the optimal conditions, the linear range of the electrochemical immunosensor was 0.1–100 μg mL−1, and the detection limit was 0.950 μg mL−1. In addition, the fabricated sensor exhibited high selectivity and long-term stability. The recovery test showed that the prepared sensor was suitable for the detection of gliadin in real food and feed, exhibiting great promising of the developed sensor in practical application. [ABSTRACT FROM AUTHOR]