1. Rice floury endosperm26 encoding a mitochondrial single-stranded DNA-binding protein is essential for RNA-splicing of mitochondrial genes and endosperm development.
- Author
-
Teng X, Wang Y, Liu L, Yang H, Wu M, Chen X, Ren Y, Wang Y, Duan E, Dong H, Jiang L, Zhang Y, Zhang W, Chen R, Liu S, Liu X, Tian Y, Chen L, Wang Y, and Wan J
- Subjects
- DNA-Binding Proteins genetics, DNA-Binding Proteins metabolism, Genes, Mitochondrial, Mitochondria metabolism, Mitochondria genetics, Gene Expression Regulation, Plant, Oryza genetics, Oryza metabolism, Oryza growth & development, Endosperm genetics, Endosperm metabolism, Endosperm growth & development, Plant Proteins genetics, Plant Proteins metabolism, RNA Splicing
- Abstract
Endosperm, the major storage organ in cereal grains, determines the grain yield and quality. Mitochondria provide the energy for dry matter accumulation, in the endosperm development. Although mitochondrial single-stranded DNA-binding proteins (mtSSBs) play a canonical role in the maintenance of single-stranded mitochondrial DNA, their molecular functions in RNA processing and endosperm development remain obscure. Here, we report a defective rice endosperm mutant, floury endosperm26 (flo26), which develops abnormal starch grains in the endosperm. Map-based cloning and complementation experiments showed that FLO26 allele encodes a mitochondrial single-stranded DNA-binding protein, named as mtSSB1.1. Loss of function of mtSSB1.1 affects the transcriptional level of many mitochondrially-encoded genes and RNA splicing of nad1, a core component of respiratory chain complex I in mitochondria. As a result, dysfunctional mature nad1 led to dramatically decreased complex I activity, thereby reducing ATP production. Our results reveal that mtSSB1.1 plays an important role in the maintenance of mitochondrial function and endosperm development by stabilizing the splicing of mitochondrial RNA in rice., Competing Interests: Declaration of Competing Interest The authors declare no conflict of interest., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF