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The Bi 2 Sr 2 CaCu 2 O 8+ x (Bi-2212) material, a high-temperature superconductor, holds significant promise for future high-field applications because of its multifilamentary, twistable production capabilities, coupled with a high upper critical field. Bi-2212 round wire (RW) is made by the powder-in-tube (PIT) technique, and Bi-2212 coils are usually being developed using the wind-and-react method and a high temperature and over-pressure (OP) heat treatment (50 atm, 890℃) is then required to achieve the high current performance. However, over-pressure (OP) heat treatment can lead to a reduction in wire diameter, compromising the stability of the coil structure. Addressing this issue, the pre-overpressure (pre-OP) heat treatment is used and proves effective in mitigating the reduction in Bi-2212 wire diameter. In this paper, to comprehensively investigate the impact of pre-OP heat treatment on the microstructure of Bi-2212 wire, a metallographic analysis of two kinds of Bi-2212 wires was carried out by the scanning electron microscope (SEM) and the ImageJ software. It was found that larger-area filaments experienced a greater reduction in area after pre-OP heat treatment, that pre-OP heat treatment increases the aspect ratio of filaments, and polygonal bundle structure helps maintain filament structure stability during pre-OP heat treatment. Additionally, the impact of pre-OP heat treatment on the performance of Bi-2212 wires was analyzed through critical current testing under high fields. [ABSTRACT FROM AUTHOR]