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A Gram-stain-negative bacterium, designated LG-2 T , was isolated from sludge collected at a pesticide-manufacturing factory in Jiangsu Province, PR China. Cells of strain LG-2 T were strictly aerobic, non-motile and spherical. Growth was observed at 15-42 °C (optimum, 30 °C), pH 6.0-9.0 (optimum, pH 7.0) and 0-3.0 % (w/v) NaCl (optimum, 1.0 %). LG-2 T showed 95.5-96.9 % 16S rRNA sequence similarity to type strains in the genera Pusillimonas , Bordetella , Parapusillimonas , Candidimonas and Paracandidimonas of the family Alcaligenaceae . The phylogenomic tree indicated that strain LG-2 T was clustered in the family Alcaligenaceae and formed a clade with Paracandidimonas soli IMT-305 T , while the phylogenetic trees based on 16S rRNA gene sequences indicated that strain LG-2 T formed a distinct clade within the family Alcaligenaceae . The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between LG-2 T and its closely related type strains in the genera Pusillimonas , Bordetella , Parapusillimonas , Candidimonas and Paracandidimonas were 70.8-75.3, 18.9-23.7 and 59.6 %-69.3 %, respectively. The major cellular fatty acids were C 16 : 0 , C 17 : 0 cyclo, summed feature 3 (C 16 : 1  ω7 c and/or C 16 : 1  ω6 c ), summed feature 8 (C 18 : 1  ω7 c and/or C 18 : 1  ω6 c ) and summed feature 2 (C 12 : 0 aldehyde and/or unknown 10.928). The predominant menaquinone was Q-8. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, two aminophospholipids, three aminolipids and nine unknown polar lipids. The genome size of strain LG-2 T was 3.2 Mb and the DNA G+C content was 63.4 mol%. On the basis of the phenotypic, phylogenetic and genomic results from this study, strain LG-2 T represents a novel species of a new genus in the family Alcaligenaceae , for which the name Yanghanlia caeni gen. nov., sp. nov. is proposed, with strain LG-2 T (=KCTC 8084 T = CCTCC AB 2023123 T ) as the type strain.

A Gram-stain-negative bacterium, designated LG-4 T , was isolated from sediment of Qiantang River in Zhejiang Province, PR China. Cells were strictly aerobic, non-spore-forming, non-motile and short-rod-shaped (1.0-1.2 µm long and 0.7-0.8 µm wide). Growth occurred at 15-42 °C (optimum, 30 °C), at pH 5.0-9.0 (pH 7.0) and at 0-2.0 % (w/v) NaCl (optimum, 0.5 % NaCl). Strain LG-4 T showed 95.75-96.90 % 16S rRNA gene sequence similarity to various type strains of the genera Tabrizicola , Pseudotabrizicola , Phaeovulum , Rhodobacter and Wagnerdoeblera of the family Paracoccaceae , and the most closely related strain was Tabrizicola soli ZQBW T (96.90 % similarity). The phylogenomic tree showed that strain LG-4 T clustered in the family Paracoccaceae and was positioned outside of the clade composed of the genera Wagnerdoeblera and Falsigemmobacter . The average nucleotide identity and digital DNA-DNA hybridization values between strain LG-4 T and the related type strains were in the range of 74.19-77.56 % and 16.70-25.80 %, respectively. The average amino acid identity (AAI) values between strain LG-4 T and related type strains of the family Paracoccaceae were 60.94-69.73 %, which are below the genus boundary (70 %). The evolutionary distance (ED) values between LG-4 T and the related genera of the family Paracoccaceae were 0.21-0.34, which are within the recommended standard (≥0.21-0.23) for defining a novel genus in the family Paracoccaceae . The predominant cellular fatty acids were C 18 : 1  ω7 c , C 19 : 0 cyclo ω8 c , C 18 : 0 and C 16 : 0 , the isoprenoid quinone was Q-10, and the major polar lipids were phospholipid, phosphatidylglycerol, phosphatidylcholine, aminolipid and two unknown polar lipids. The genome size was 4.7 Mb with 68.6 mol% G+C content. On the basis of distinct phylogenetic relationships, low AAI values and high ED values, and differential phenotypic, physiological and biochemical characteristics, strain LG-4 T represents a novel species of a new genus in the family Paracoccaceae , for which the name Ruixingdingia sedimenti gen. nov., sp. nov. is proposed. The type strain is LG-4 T (=MCCC 1K08849 T =KCTC 8136 T ).