Search

Objective To investigate the relationship between serum level of pyruvate kinase M2 (PKM2), galectin-3(Gal-3) and citrolinated histone H3 (CitH3) and pulmonary infection in patients with mechanical ventilation (MV). Methods A total of 120 patients with MV admitted to Jinhua Central Hospital from October 2022 to March 2024 were included in the study. They were divided into pulmonary infection group (n=50) and non-pulmonary infection group (n=70). Serum level of PKM2, Gal-3 and CitH3 was detected by ELISA. The patients were divided into good prognosis group (n=79) and bad prognosis group (n=41). Multivariate Logistic regression analysis was performed to analyze the factors affecting the adverse prognosis of MV patients. Receiver operating characteristic (ROC) curve was used to analyze the predictive value of serum PKM2, Gal-3 and CitH3 for poor prognosis in patients undergoing MV. Results Compared with non-pulmonary infection group, serum level of PKM2, Gal-3 and CitH3 in pulmonary infection group was increased (P＜0.05). The level of serum PKM2, Gal-3 and CitH3 in the poor prognosis group was increased (P＜0.05). The Clinical Pulmonary Infection score (CPIS) of the poor prognosis group was higher than that of the good prognosis group (P＜0.05). PKM2, Gal-3 and CitH3 were all risk factors for poor prognosis in MV patients (P＜0.05). The area under the curve (AUC) of PKM2, Gal-3, CitH3 and combined prediction of poor prognosis were 0.712, 0.839, 0.779 and 0.925, respectively. AUC of combined diagnosis of PKM2, GAL-3, CITH3 and combined diagnosis was better than that of single detection (Z=4.261, 2.521, 3.676, P＜0.001, 0.05, 0.001). Conclusions The serum level of PKM2, Gal-3, and CitH3 in MV patients with concurrent pulmonary infections is found to be higher than those in those without pulmonary infections, so these three factors have potential predictive value for poor prognosis of MV patients. [ABSTRACT FROM AUTHOR]

Objective To investigate serum β2-MG, sCHE, and PSGL-1 expression in patients with esophageal cancer and their relationship to lung infection after mediastinoscopy. Methods A total of 118 patients with esophageal cancer were selected and divided into infected and uninfected groups according to whether they developed lung infection after surgery. An automatic microbiological identification system was used to detect the pathogenic bacteria of lung infection. ELISA was used to detect the levels of β2-MG, sCHE, and PSGL-1. Multivariate logistic regression was used to analyze the influencing factors of postoperative lung infection in patients with esophageal cancer. ROC curves were plotted to analyze the assessment value of serum β2-MG, sCHE, and PSGL-1 on postoperative lung infection. Results Fifty-two strains of bacteria were isolated from the sputum of 38 patients with postoperative lung infections, and these included 35 (67.31%) Gram-negative, 14 (26.92%) Gram-positive, and 3 (5.77%) fungal strains. The difference in long-term smoking history between the infected and uninfected groups was statistically significant (P<0.05). Serum β2-MG and PSGL-1 levels were significantly higher and sCHE levels were significantly lower in the infected group than in the uninfected group (P<0.05). Serum β2-MG and PSGL-1 levels were sequentially higher (P<0.05) and sCHE levels were sequentially lower (P<0.05) in the mild, moderate, and severe lung infection groups. Long-term smoking history, β2-MG, and PSGL-1 were risk factors affecting postoperative lung infection in patients with esophageal cancer (P<0.05), and sCHE was a protective factor (P<0.05). The AUCs of serum β2-MG, sCHE, and PSGL-1 for assessing postoperative lung infections were 0.807, 0.845, and 0.800, respectively, and the AUC of the three combined factors for assessing postoperative lung infections was 0.954, which was superior to that assessed individually (Zcombination vs. β2-MG=2.576, Zcombination vs. sCHE=2.623, Zcombination vs. PSGL-1=2.574, all P<0.05). Conclusion The serum levels of β2-MG and PSGL-1 increase and the sCHE level decreases in patients with esophageal cancer and postoperative pulmonary infection, which are also related with lung infection. Combined testing can improve the evaluation value of postoperative pulmonary infection in patients. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

目的: 分析BLUE超声在基层医院肺部感染诊断中的应用效果。方法: 选取2024年02月-2024年11月本 院65例疑似肺部感染患者开展研究, 均进行多层螺旋CT (MSCT) 检查, 以此为金标准, 实施BLUE超声检查, 分析 BLUE超声诊断效果。结果: MSCT检出62例阳性, 3例阴性, BLUE超声分别为60例和5例, BLUE超声的诊断准确率 为92.31%, 特异度为66.67%, 敏感度为95.16%; 主要超声图像: 肺滑动征消失2例, 占比3.08%, 胸腔积液1例, 占比 1.54%, 肺不张1例, 占比1.54%, 支气管充气征4例, 占比6.15%, 肺泡间质综合征6例, 占比9.23%, B线25例, 占比 38.46%, 异常胸膜线18例, 占比27.69%。结论: 基层医院检查肺部感染时, 采用BLUE超声, 有较高诊断效能, 超声 图像清晰、明显, 值得推广。 [ABSTRACT FROM AUTHOR]