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Objective To explore the clinical effect of hair transplantation with autologous follicle on pubic hair reconstruction in women with loss of pubic hair. Methods A total of 38 patients with pubichair loss treated in Hair Transplantation Center of Plastic Surgery Hospital of Chinese Academy of Medical Sciences & Peking Union Medical College were recruited. The scalp strips from the safe donor area were taken and hair transplantation of autologous follicles was conducted for reconstruction. For patients with congenital pubic hair loss, according to the characteristics of female pubic hair distribution and the patient's own requirements, the inverted triangle design was used. For perimenopausal women, the shape of residual pubic hair was considered for design of the region of hair transplantation. For patients with scars, besides scar area implantation was paid attention to, it was also important to design the implantation area above the scar area according to the conditions in order to further cover the scar area with the implanted pubic hair. Results Thirty-eight patients were followed-up for 6 to 24 months. The hairs transplanted grew well. The appearance was close to the normal distribution of pubic hair, resulting in a satisfactory effect. Conclusion It is suggested to reconstruct female pubic hair loss with autologous follicles transplantation. [ABSTRACT FROM AUTHOR]