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Background: Chitin-synthesis inhibitors (CSIs) represent a major tool in vector control. The intensive use of these compounds has led to the evolution of resistance against several CSIs, including diflubenzuron (DFB). DFB resistance has been associated to a target-site mechanism; however, studies investigating the gene expression profile of resistant phenotypes are limited, preventing a full understanding of DFB resistance. Here, we analyzed the constitutive gene expression of susceptible and DFB-resistant individuals of the mosquito Culex pipiens, a major disease vector in temperate areas., Results: Comparative gene expression analysis between susceptible and DFB-resistant individuals identified 527 differentially expressed genes (i.e., 432 up-regulated and 95 down-regulated genes). Among the up-regulated genes, 87 genes belong to gene families associated with insecticide resistance in arthropods, such as cytochrome P450s, glutathione-S-transferases, UDP-glucuronosyltransferases, heat shock proteins and cuticular proteins (CPs). Interestingly, the CP transcripts were the most abundant among up-regulated genes (73 of 87), and furthermore they constitute 11 of the 20 most over expressed genes. The enrichment of transcripts associated with cuticle synthesis was also identified by the Gene Ontology (GO) enrichment analysis., Conclusions: Adaptation to insecticides can involve transcriptional changes in genes encoding for multiple defense mechanisms. Our results identified the over-expression of transcripts associated with detoxification and cuticle synthesis in DFB-resistant individuals of Cx. pipiens. Multiple mechanisms, beyond the known target-site mechanism, may therefore contribute to the DFB-resistant phenotype. Together these findings corroborate the complexity underpinning the resistant phenotypes and provide important information for the implementation of effective control strategies against mosquito vectors. © 2025 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry., (© 2025 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.)

The lesser mealworm Alphitobius diaperinus Panzer (Coleoptera: Tenebrionidae), an important insect pest of stored-product commodities and poultry production systems, is regarded among the most difficult species to control. It has developed resistance to many chemical insecticides, and though various cultural and biological methods have been assessed and identified as possible factors for its control, none are currently implemented. Entomopathogenic nematodes are often successfully employed as alternative to chemicals biocontrol agents of various insect species, including pests of stored products; nevertheless, their evaluation as potential biocontrol factors of the lesser mealworm is not efficiently scrutinized. In the current study, single A. diaperinus larvae were exposed to six doses of Heterorhabditis bacteriophora Poinar (Rhabditida: Heterorhabditidae), Steinernema carpocapsae (Weiser) (Rhabditida: Steinernematidae), and Steinernema feltiae (Filipjev) (Rhabditida: Steinernematidae), for 4 and 8 d, and mortality was recorded at 3 different temperature regimes, i.e., 25 oC, 30 oC, and 35 oC. The study concludes that S. carpocapsae and S. feltiae are both highly virulent against A. diaperinus larvae and may be considered as promising biological control agents for reducing lesser mealworm infestations when applied at a rate of 70 IJs/cm2 at 25 oC. When assessed at 30 oC, both species were effective at the same rate though causing lower mortality of ~60% and ~50%, respectively, whereas their efficacy was low at 35 oC., (© The Author(s) 2024. Published by Oxford University Press on behalf of Entomological Society of America.)

Over the past two decades, many double-stranded (ds)-RNAs have been synthesized to silence target genes for exploration of gene functions in pests. Some of these dsRNAs are lethal to pests, leading to a new category of pesticides. The generation of these environmentally friendly pesticides requires precise in silico design of dsRNA molecules that target pests but not non-pest organisms. Current efforts in dsRNA design focus mainly on the analysis of the target gene sequence, lacking comprehensive analysis of all transcripts of the whole transcriptome per given species, causing low efficiency and imprecise dsRNA target exploration. To address these limitations, we created the dsRNAEngineer online platform (https://dsrna-engineer.cn), which allows comprehensive and rational dsRNA design, incorporating hundreds of pest and non-pest transcriptomes. Developed functionalities include screen-target (screen conserved genes for cotargets of various pest species), on-target, off-target, and multi-target to generate optimal dsRNA for precise pest control., Competing Interests: Declaration of interests The authors declare no competing interests., (Copyright © 2025 Elsevier Ltd. All rights reserved.)

Plant viruses utilize a subset of host plasmodesmata-associated proteins to establish infection in plants. In the present study, we aimed to understand the role of two plant genes, one encoding a putative plasmodesma located protein (PDLP) and a homolog of soybean gene regulated by cold 2 protein (SRC2) during Cucumber mosaic virus (CMV) infection. Virus-induced gene silencing (VIGS) was used to silence PDLP and SRC2 genes in Nicotiana benthamiana and in two related solanaceous plants, N. tabacum and Capsicum chinense Jacq. (Bhut Jolokia). Up to 50% downregulation in the expression of the PDLP gene using the TRV2- PDLP VIGS construct was observed in N. benthamiana and N. tabacum while, using the same gene construct, 30% downregulation of the target mRNA was observed in C. chinense . Similarly, using the TRV2- SRC2 VIGS construct, a 60% downregulation of the SRC2 mRNA was observed in N. benthamiana , N. tabacum , and a 40% downregulation in C. chinense as confirmed by qRT-PCR analysis. Downregulation of the PDLP gene in N. benthamiana resulted in delayed symptom appearance up to 7-12 days post inoculation with reduced CMV accumulation compared to the control plants expressing TRV2- eGFP . In contrast, SRC2 -silenced plants showed enhanced susceptibility to CMV infection compared to the control plants. Our data suggest that the PDLP gene might facilitate infection of CMV, thus being a susceptibility factor, while the SRC2 gene could play a role in resistance to CMV infection in N. benthamiana .

Grapevine trunk disease (GTD) is a major threat to grapevine propagation, severely affecting the growth and development of young vines. As one of the most destructive plant diseases in the world, GTD spreads easily through propagation material and threatens the sustainability of vineyards. While effective, biologically friendly treatments remain unavailable. This study investigated the graft yield, the growth potential of grapevine hetero-grafts, and phenolic responses focusing on (i) GTD scion health status (healthy-HLT; asymptomatic-ASYM; symptomatic-SYM) and (ii) disinfection methods. Grafting with HLT scions achieved the highest yield rates, particularly with Serenade ® ASO (75%) and BioAction ES (79%), while infected scions showed lower yields. The growth potential of the scions was not affected by the disinfection method or the health status of the scions. Phenolic composition varied between scions, graft callus, rootstock canes, and roots, with scion health status strongly influencing most metabolites. Higher levels of flavanols were observed in HLT scions treated with BioAction ES and Serenade ® ASO, with these treatments resulting in 1.6 and 1.5 times higher procyanidin dimer levels, respectively, compared to Beltanol. Flavanols and stilbenes were lower in the callus tissue of grafts with healthy scions compared to infected scions. Rootstock also showed higher levels of catechin and procyanidin dimers in grafts with HLT scions. These results indicate that the health status of scion GTD and the disinfection methods significantly influence the graft yield and phenolic composition, providing valuable insights for GTD management.

The alpine plant Cicerbita alpina (L.) Wallr., when grown as a sprout, is known as a bitter-tasting culinary delicacy. Recently it has also been reported to have anthelmintic activity, prompting further investigation into its mechanism of action. Liquid-liquid fractions were prepared from a methanolic extract of the aerial parts and were submitted in parallel to embryo development (ED), worm motility (WMT), and cytotoxicity assays for anthelmintic and toxicity evaluations. The anthelminthic assays revealed the more polar fractions to be most active against Ascaridia galli embryos (BuOH | 68% ED | c = 500 µg/ml and EtOAc | 65% ED | c = 500 µg/ml) and Caenorhabditis elegans adult worms (BuOH | 49% WMT | c = 150 µg/ml and EtOAc | 74% WMT | c = 150 µg/ml) suggesting the fraction's constituents possess dual anthelmintic activity against multiple life-cycle stages (i.e., eggs, worms) of helminths. Additionally, the BuOH fraction was non-cytotoxic to human cell-lines. Subsequent FCC and SEC derived subfractions were submitted to the anthelmintic assay workflow and the enriched subfractions B1 and E3.8, phytochemically assigned as 11-β,13-dihydrolactucin and luteolin, demonstrated bioactivity against the embryo phenotype (B1 | 58% ED | c = 1.8 µM and E3.8 | 46% ED | c = 1.7 µM) within range of the flubendazole control. Furthermore, luteolin was found to inhibit C. elegans egg hatching (luteolin | 65% EH | c = 10 µM | t = 10 h) within the range of the control albendazole. Both identified anthelmintic phytochemicals were found to affect tubulin polymerisation at a concentration of c = 50 µM. Together with in silico virtual screening studies, these results suggest microtubule stabilisation as a possible anthelmintic target and mechanism of action. This work effectively advocates the consideration of C. alpina extracts and fractions for the development of herbal therapeutics against parasitic helminths., Competing Interests: Declarations. Competing interests: The authors declare no competing interests., (© 2024. The Author(s).)

This study evaluates the impact of yellow mealworm frass in combination with an inorganic nitrogen fertilizer on growth, yield, and nutrient concentration in annual sowthistle plants. It was found that the combined application of yellow mealworm frass (YM-frass) as basal fertilizer and inorganic nitrogen fertilizer (INF) as top dressing increased the shoot biomass of annual sowthistle up to 67.2 % compared to the INF treatment (100 kg N/ha). The application of both YM-frass and INF increased P, Mg and Fe root concentration. Moreover, the lowest P and K concentration in shoot tissues were found in the unfertilized control treatment, while the reverse was the case for Ca and Fe. In conclusion, yellow mealworm frass can be used instead of inorganic basal fertilizer, whereas its combination with an inorganic nitrogen fertilizer applied as top-dressing during the vegetative growth can increase the yield and quality of leafy vegetables., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier Ltd.)

Arabinoxylan is a prevalent hemicellulose type, notably heterogeneous and resistant to biodegradation. Arabinofuranosidases (Abfs) remove arabinofuranosyl decorations of arabinoxylan, thus enabling hydrolysis by xylanases. However, a variety of Abf and xylanase specificities have emerged in recent years, necessitating a deeper understanding of their role in biomass degradation. This work investigates the biochemical features of TtAbf43 from Thermothelomyces thermophila, which specifically removes the O-3-linked arabinofuranose moieties from di-substituted xylopyranoses. The enzyme also exhibited secondary hydrolytic activity on o-nitrophenyl-β-d-xylopyranoside and arabinan. The hydrolysis of pretreated wheat and corn bran substrates was assessed using TtAbf43 and AnAbf51, two enzymes with distinct catalytic specificities. The Abfs enhanced the performance of endo-xylanases TmXyn10 and AnXyn11, promoting the release of xylo-oligomers, while the xylanases, in turn, stimulated arabinose release by the Abfs. Additionally, the Abfs facilitated the endo- and exo-activities of the bifunctional xylobiohydrolase/glucuronoxylanase TtXyn30A for the release of xylobiose and short aldouronic acids from biomass. AnAbf51 also acted in synergy with the acetyl xylan esterase OCE6 and the exo-deacetylase TtCE16B in debranching enzymatically derived oligomers from lignocellulose, whereas TtAbf43 remained unaffected by the esterases. These diverse synergistic relationships among different hemicellulases could assist the development of new enzymatic approaches for efficient biomass valorization., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2025 The Authors. Published by Elsevier B.V. All rights reserved.)

Objective: Overweight and obesity are characterized by excess adiposity and systemic, chronic, low-grade inflammation, which is associated with several metabolic disorders. The aim of this study was to assess the feasibility and tolerability of β-alanine supplementation and to explore the effects on cardiometabolic health and cardiovascular, hepatic, and renal function in adults with overweight and obesity., Methods: A total of 27 adults (44% female; mean [SD], age: 58 [10] years, BMI: 31.1 [2.9] kg/m 2 , hemoglobin A1c: 39.8 [4.3] mmol/mol) received β-alanine (4.8 g/day) or a matched placebo for 3 months. Feasibility and tolerability outcomes included adherence, side effects, recruitment, attrition, and blinding, and exploratory outcomes included biochemical markers, blood pressures, and transthoracic echocardiography parameters. Data were analyzed using a Bayesian approach presented with 95% credible intervals (CrI)., Results: β-alanine was well tolerated and adhered to (adherence: placebo, 0.91 [95% CrI: 0.84-0.95]; β-alanine, 0.92 [95% CrI: 0.85-0.95]), and side effects remained at or below baseline throughout. The probability that β-alanine supplementation affected cardiometabolic, cardiovascular, or clinical biochemical outcomes was low., Conclusions: Sustained-release β-alanine supplementation is well tolerated and adhered to in adults with overweight and obesity. Future research should consider more advanced metabolic conditions, which may benefit from longer duration supplementation., (© 2025 The Author(s). Obesity published by Wiley Periodicals LLC on behalf of The Obesity Society.)

Introduction: This study aimed to evaluate, using the in vitro gas production technique, the effect of including eight agro-industrial by-products (carob, grape, two types of olive pomace, citrus pulp, tomato, and hazelnut skin) on fermentation end-products, ruminal degradability, and methane production in sheep diets., Methods: The by-products were included at 10% dry matter in the control (CTR) diet, commonly adopted for adult sheep (80% natural grassland and 20% concentrate), and incubated at 39°C under anaerobic conditions., Result and Discussion: After 24 h of the incubation, the organic matter degradability (OMD24h) and methane production were assessed. After 120 h of the incubation, the organic matter degradability (OMD120h), volume of gas produced (OMCV), fermentation kinetics, pH, volatile fatty acids (VFAs), and ammonia were evaluated. Dunnett's test was used to compare the differences between the control and experimental diets, and multivariate analysis was performed to highlight the differences among the diets based on their in vitro characteristics. The results indicated that the inclusion of the by-products decreased the degradability and increased gas production after 120 h of the incubation. The by-products from the hazelnuts, citrus, grapes, and tomatoes significantly ( p < 0.001) reduced the methane production, whereas the pomegranate, grape, 3-phase olive cake, tomato, and hazelnut by-products significantly ( p < 0.001) increased the acetate production. The multivariate analysis showed that the butyrate concentration was a determining factor in the differences between the diets. The concentration of polyphenols in the selected agro-industrial by-products could modify fermentation parameters and metabolic pathways, leading to reduced methane production., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision., (Copyright © 2025 Vastolo, Mora, Kiatti, Nocerino, Haroutounian, Baka, Ligda, Cutrignelli, Niderkorn and Calabrò.)

The prominence of binuclear, bimetallic catalysts underlines the need for the design and development of diverse bifunctional ligand frameworks that exhibit tunable electronic and structural properties. Such strategies enable metal-metal and ligand-metal cooperation towards catalytic applications, improve catalytic activity, and are essential for advancing multi-electron transfers for catalytic application. In this work we present the synthesis, crystal structure, and photocatalytic properties of a binuclear Ni(II) complex, [Ni 2 (1,10-phenanthroline) 2 (2-sulfidophenolate) 2 ] (1). Complex 1 crystallizes in the centrosymmetric triclinic system (P-1) showing extensive intra- and inter- non-coordinated interactions. 1 is employed as a catalyst for light driven hydrogen evolution. Its catalytic efficiency in a noble-metal-free photo-driven system using fluorescein as photosensitizer and triethanolamine as the electron donor, reaches TON 2900, threefold the efficiency of the corresponding homoleptic mononuclear complex [Ni(2-sulfidophenolate) 2 ]. Efficiency rises up to 9000 TONs when thioglycolic-coated CdTe quantum dots are used as photosensitizers in the presence of ascorbic acid at pH 4.5. UV-Vis spectroscopy, dynamic light scattering techniques, and Hg-poisoning measurements reveal that 1 maintains its molecular structure during catalysis. Electrochemical studies in DMF with TFA as the proton source were also performed for the elucidation of the mechanism of its catalytic action and its stability, suggesting that the proximity of two nickel ions plays a part in the increased catalytic activity, facilitating hydrogen evolution., (© 2025 The Author(s). Chemistry - A European Journal published by Wiley-VCH GmbH.)

The most common and damaging plant parasitic nematodes are root-knot nematodes (RNK). Although hemp has been clearly infected by RNK, little information is available regarding the extent of the damage and losses caused. In addition, no information is available concerning hemp seed extracts' activity against RNK. In the current research, a greenhouse experiment was developed to evaluate the infection effect of five RKN species ( Meloidogyne javanica , M. incognita , M. arenaria , M. hapla , and M. luci ) in nine industrial hemp varieties (Fedora 17, Ferimon 12, Futura 75, Santhica 27, Santhica 70, KC Dora, KC Zuzana, Zenit, and USO 31), as was a laboratory experiment to evaluate the nematicidal activity of aqueous and water extracts of hemp seeds against second-stage juveniles (J2s) of M. javanica . The results revealed that the five evaluated Meloidogyne species were pathogenic to hemp plants. The lowest shoot weights were recorded in plants that were inoculated with M. javanica and M. luci (4.65 and 4.53 g, respectively). As for the root system, the most severe damage of the roots was detected in M. incognita -infected plants, presenting the lowest root weight (1.72 g). Regarding hemp varieties, the most susceptible were USO 31, Fedora 17, Ferimon 12, and Zenit, while Futura 75 hosted the lowest nematode numbers, including the lowest values of females per gram of root (10.66), number of egg masses per gram of root (4.70), and fecundity (33.68 eggs per egg mass). Moreover, this research showed that aqueous and ethanolic hemp seed extracts can control M. javanica . After 24, 48, 72, and 96 h of exposure to high concentrations (250-2000 ppm) of ethanolic and aqueous extracts, the J2s were permanently paralyzed; however, at low concentrations, only the ethanolic extract was effective. The ethanolic extract paralyzed J2s at concentrations higher than 62.5 ppm and reduced the egg hatching by up to 76% compared to the control treatment. In general, the efficacy of the aqueous extract was considerably lower than that of the ethanolic extract.

Background/objectives: The effect of maternal macronutrient composition on the risk of large for gestational age (LGA) neonates among women with gestational diabetes mellitus (GDM) is not well understood. This study aimed to investigate these associations in a pregnant cohort in Northern Greece, considering both pre-pregnancy and early pregnancy dietary intake, and stratifying women by pre-pregnancy body mass index (BMI)., Methods: From a total of 797 eligible pregnant women, the 117 (14.7%) who developed GDM (and thus were included in the study) completed the validated Food Frequency Questionnaires (FFQs). Macronutrient intake was assessed for the six months before pregnancy and until mid-gestation, prior to the oral glucose tolerance test. Data were compared with European Food Safety Authority (EFSA) guidelines, and participants were stratified by pre-pregnancy BMI (normal vs. overweight/obese). Multivariate logistic regression was used to estimate adjusted odds ratios (aORs) for LGA risk., Results: In normal-BMI women with GDM, higher dietary fiber (aOR = 1.39) and vegetable protein (aOR = 1.61) intake before pregnancy were both significantly associated with an increased risk of LGA. During early pregnancy, the elevated risk from vegetable protein persisted (aOR = 1.51). Among overweight/obese women, no significant pre-pregnancy associations were observed. However, during early pregnancy, a higher percentage of total carbohydrate intake was linked to increased LGA risk (aOR = 1.11), while maintaining saturated fatty acids "as low as possible" reduced the odds of LGA (aOR = 0.71). Elevated vegetable protein intake also increased LGA risk (aOR = 1.61)., Conclusions: Maternal macronutrient intake prior to and during early pregnancy may influence LGA risk in GDM, with distinct patterns according to pre-pregnancy BMI. These findings underscore the importance of tailoring dietary recommendations-especially regarding fiber, vegetable protein, carbohydrates, and saturated fat-to mitigate the risk of LGA in women with GDM.

Sitophilus oryzae , Tribolium castaneum , Tribolium confusum , Oryzaephilus surinamensis , Rhyzopertha dominica , Tenebrio molitor , Trogoderma granarium , Acarus siro , and Alphitobius diaperinus represent significant arthropod stored-product pests worldwide. To combat these noxious arthropods, the current study examines the pesticidal effect of essential oils (EOs) derived from four aromatic plants, i.e., Illicium verum Hook. F., Citrus reticulata Blanco, Monodora myristica (Gaertn.) Dunal, and Xylopia aethiopica (Dunal) A. Rich. Considering the challenge of pesticide resistance, the current study focuses on assessing the efficacy of these EOs as an eco-friendly alternative to traditional synthetic insecticides. Two EO concentrations (500 and 1000 µL/kg wheat) were applied to different life stages of these pests in the bioassays. Mortality rates were monitored over several days under controlled environmental conditions. The findings demonstrated that C. reticulata and I. verum EOs had elevated insecticidal effects, especially against larval stages, resulting in 100% mortality in several species. On the contrary, M. myristica and X. aethiopica EOs showed less overall efficacy despite their potency against some pests. Both I. verum and C. reticulata EOs outperformed the positive control, pirimiphos-methyl, in several assays. The results of the current study highlight the potential of several EOs as effective alternatives in reducing synthetic pesticide use for integrated pest control management.

Plastic film, also known as low-density polyethylene (LDPE), poses serious environmental challenges due to mass production, short life cycle, and poor waste management. The main aim of this paper was to examine the suitability of using agricultural waste film as a binder in construction composites instead of the traditional cement slurry. Molten at temperatures of around 120-150 °C wastes was mixed with fine sand and gravel aggregate as filler. Twelve samples consisting of different mixtures were produced-F20, F25, F30, F35, F40, F45, F50, F60, F70, F80, F90, and F100-where a given number indicates the weight ratio of film waste to aggregate used. The composites were subjected to various tests, including volumetric density, compressive strength, and flexural strength. The volumetric density (ρ) of the composites decreased with increasing amounts of waste. Composites containing 100% recyclate (F100) depicted density, ρ = 0.74 g/cm 3 , was 50.7% lower than for a composite that contained 20% recyclate (F20). The highest soakability was recorded in F20 (2.19%). Subsequently, the absorbency tested in composites decreased with increasing recyclate content. Compression strength (σ comp ) was highest for F40 (σ comp = 39.46 MPa). In contrast, F20 had the lowest recorded compressive strength value (σ comp = 11.13 MPa) and was 71.8% lower than F40. F70 had the highest recorded flexural strength value (σ flex = 27.77 MPa). The other composites showed lower strength for higher amounts of recyclate and the amount of sand. SEM imaging proved that the contact zone between the aggregate grains and the bonding phase of the recycled film was consistent, with no anomalies, cracks, or voids. The results prove that LDPE film waste is suitable for use as a binder in building composites. However, appropriately selecting proportions of the recyclate, sand, and gravel aggregate is crucial to obtain a composite with technical parameters similar to those of cementitious composites., Competing Interests: The authors declare no conflicts of interest.

Haemosporidians, a group of vector-borne parasites that parasitize the blood cells and internal organs of various animal species, are reported to cause severe pathology in raptors. Species belonging to the genera Plasmodium, Haemoproteus and Leucocytozoon are the ones of greatest wildlife importance. The common buzzard (Buteo buteo) and the Eurasian sparrowhawk (Accipiter nisus) are the most numerous raptor species in Europe. Reliable data is lacking for many raptor species in Greece. The aim of this study was to assess, for the first time, the prevalence and geographical distribution of haemosporidian infection (mainly Leucocytozoon and Plasmodium) in these two avian species in Greece, in correlation with the risk factors of age and sex. In total, 62 common buzzards and 26 Eurasian sparrowhawks were included in this study, all being admitted for treatment at a Greek Wildlife Rehabilitation Center. Blood samples were collected and microscopical analysis was performed after staining blood smears with Giemsa. DNA was extracted from each sample and a fraction of the mitochondrial cytochrome b gene was amplified by a nested PCR protocol. All positive samples were subjected to sequencing. Total prevalence of haemosporidian infection by morphological and molecular examination was 59% and 73.9%, respectively. Binary logistic regression was carried out. The most prevalent infection was by Leucocytozoon spp. Most of the samples had mixed infections. The isolated genetic lineages of Leucocytozoon spp. were BUBT2, BUBT3, MILVUS01, ACNI1, BUBO01 and MILANS04. The detected genetic lineages of Plasmodium spp. were TURDUS1, BT7 and DONANA02. A new genetic lineage, BUTBUT17, was also identified., Competing Interests: Declarations. Ethics approval: Ethical approval was obtained by the Ethics Committee of the Aristotle University of Thessaloniki (32778/2021). Collection of blood samples was carried out by veterinarians adhering to the regulations and guidelines on animal husbandry and welfare. The birds were admitted to ANIMA, Association for Wildlife Care and Protection which is a nationally and legally recognised non-profit association for the hospitalization and rehabilitation of wild animals. Consent to participate: All authors consent to participate in this publication. Consent for publication: All authors consent to publish the manuscript. Competing interests: The authors declare no competing interests. Clinical trial number: Not applicable., (© 2025. The Author(s).)

One of the main challenges in aquaculture is the constant search for sustainable alternative feed ingredients that can successfully replace fishmeal (FM) without any negative effects on fish growth and health. The goal of the present study was to develop a toolbox for rapidly anticipating the dynamics of fish growth following the introduction of a new feed; nonlethal, biochemical, and molecular markers that provide insights into physiological changes in the fish. A nutritional challenge by feeding a conventional feed rich in FM protein (FM diet) versus an experimental feed rich in plant protein (PP) and low FM inclusion (PP diet), in 20 different families of gilthead sea bream ( Sparus aurata ) was performed. Fifteen and 30 days after the initiation of the nutritional challenge, the transcriptional response of gilthead seabream erythrocytes along with classical hematological biochemical markers were compared. Zootechnical, biochemical, and transcriptome data from each family under different dietary treatments were combined into a classification model to identify variables that can predict the growth rate at the end of the 14-month farming period (July 2018-September 2019). A highly accurate model was produced ( A  > 80%) based on the combination of seven markers (five molecular and two biochemical markers) and with high potential in separating faster and slower growing fish as early as 30 days after the initiation of feeding., Competing Interests: The authors declare no conflicts of interest., (Copyright © 2025 Rafael Angelakopoulos et al. Aquaculture Nutrition published by John Wiley & Sons Ltd.)

Eicosanoids are key players in inflammatory diseases and cancer. Targeting their production by inhibiting Group IVA cytosolic phospholipase A 2 (cPLA 2 α) offers a promising approach for cancer therapy. In this study, we synthesize a second generation of thiazolyl ketone inhibitors of cPLA 2 α starting with compound GK470 (AVX235) and test their in vitro and cellular activities. We identify a more potent and selective lead molecule, GK420 (AVX420), which we test in parallel with AVX235 and a structurally unrelated compound, AVX002 for inhibition of cell viability across a panel of cancer cell lines. From this, we show that activity of polycomb group repressive complex 2 is a key molecular determinant of sensitivity to cPLA 2 α inhibition, while resistance depends on antioxidant response pathways. Consistent with these results, we show that elevated intracellular reactive oxygen species and activating transcription factor 4 target gene expression precede cell death in AVX420-sensitive T-cell acute lymphoblastic leukemia cells. Our findings imply cPLA 2 α may support cancer by mitigating oxidative stress and inhibiting tumor suppressor expression and suggest that AVX420 has potential for treating acute leukemias and other cancers that are susceptible to oxidative cell death., Competing Interests: Competing interests: A.J.F., B.J., F.J.A., E.A.D., and G.K. are shareholders, while F.J.A. and N.M. were employees of Coegin Pharma at the time of results generation. The other authors declare no conflict of interest. The funders had no role in the design of the study, in the collection, analyses, or interpretation of data, in the writing of the manuscript, or in the decision to publish the results., (© 2024. The Author(s).)

Background/Objectives: Maternal amino acid intake and its biological value may influence glucose regulation and insulin sensitivity, impacting the risk of developing gestational diabetes mellitus (GDM). This study aimed to evaluate the association between amino acid intake from maternal diet before and during pregnancy and the risk of GDM. Methods: This study is part of the ongoing BORN2020 epidemiological Greek cohort. A validated semi-quantitative Food Frequency Questionnaire (FFQ) was used. Amino acid intakes were quantified from the FFQ responses. A multinomial logistic regression model, with adjustments made for maternal characteristics, lifestyle habits, and pregnancy-specific factors, was used. Results: A total of 797 pregnant women were recruited, of which 14.7% developed GDM. Higher cysteine intake during pregnancy was associated with an increase in GDM risk (adjusted odds ratio [aOR]: 5.75; 95% confidence interval [CI]: 1.42-23.46), corresponding to a 476% increase in risk. Additionally, higher intakes of aspartic acid (aOR: 1.32; 95% CI: 1.05-1.66), isoleucine (aOR: 1.48; 95% CI: 1.03-2.14), phenylalanine (aOR: 1.6; 95% CI: 1.04-2.45), and threonine (aOR: 1.56; 95% CI: 1.0-2.43) during pregnancy were also associated with increased GDM risk. Furthermore, total essential amino acid (EAA) (aOR: 1.04; 95% CI: 1.0-1.09) and non-essential amino acid (NEAA) (aOR: 1.05; 95% CI: 1.0-1.1) intakes during pregnancy were also linked to an increased risk of GDM. A secondary dose-response analysis affected by timing of assessment revealed that higher intake levels of specific amino acids showed a more pronounced risk. Conclusions: Optimizing the balance of certain amino acids during pregnancy may guide personalized nutritional interventions to mitigate GDM risk.

N-Phenylacetyl-L-prolylglycine ethyl ester (Noopept, GVS-111, omberacetam) is an orally available active pharmaceutical ingredient (API), with neuroprotective properties and ability to enhance cognitive function. It belongs to nootropic family of drugs and is included in the group of racetams, although its chemical structure is quite different than the other compounds from this group, including the most popular one - piracetam. The mechanism of action of this API is multifaced and is considered to be involving modulation of various neurotransmitter systems within the brain. Despite the significant amount of works devoted to the pharmacodynamics of Noopept, very little is known about its structural and physicochemical properties. Therefore, the aim of current study was to investigate this API in a very thorough way. In this work, the detailed physicochemical analysis of Noopept has been done using TGA/DSC, 1 H and 13 C liquid state NMR, 13 C CP/MAS NMR, SEM, SCXRD, and PXRD. Additionally, quantum chemical DFT computations under periodic boundary conditions, using CASTEP, were conducted to facilitate the analysis of experimental results. Besides, we've performed a polymorphism screening of this molecule., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Background and Objectives: Substance use disorders (SUD) are a major public health concern in the United States. This study examined racial/ethnic and state-level disparities in SUD mortality in the United States from 2000 to 2019., Methods: Age-standardized mortality rates for SUD were obtained for 5 racial/ethnic groups (White respondents, Black respondents, Latino, Asian-Pacific Islander [API], American Indian/Alaska Native [AIAN]) by state and sex from 2000 to 2019. Joinpoint regression analysis was used to model temporal trends overall and by demographic factors., Results: From 2000 to 2019, the overall mortality rate increased from 8.0 to 28.8 deaths per 100,000 population across all groups. AIANs had the highest mortality in 2019 (57.8 per 100,000), followed by Black respondents, White respondents, Latinos, and APIs. Significant increases occurred across all racial/ethnic groups, with the greatest average annual percentage change (AAPC 2000-2019 ) among White respondents (6.7%; 95% confidence interval [CI]: 6.2%-7.3%), APIs (6.0%, 95% CI: 5.6%-6.2%), and AIANs (5.9%, 95% CI: 5.6%-6.2%). Mortality rates increased more rapidly for females than males among White respondents, AIANs, Black respondents, and Latinos. Substantial state-level variation emerged, with the highest mortality rates in 2019 seen in West Virginia, the District of Columbia, Delaware, Ohio, and Pennsylvania., Discussion and Conclusions: Racial/ethnic and geographic disparities in SUD mortality have widened significantly from 2000 to 2019, highlighting priority areas for prevention efforts., Scientific Significance: This study provides detailed insights into long-term trends in racial, ethnic, and geographic disparities in SUD mortality across the United States, informing targeted prevention and intervention strategies., (© 2024 The American Academy of Addiction Psychiatry (AAAP).)

Purpose of Review: The aim of this study was to conduct a review of the literature published over the past 18 months and present the latest findings on hydration in individuals with dementia., Recent Findings: A systematic review identified sarcopenia, polypharmacy, delayed oral transit, and poor rinsing ability as markers of eating-drinking-swallowing difficulties in early-stage dementia. A cross-sectional study found a high prevalence of dehydration (57-68%) among dementia patients, associated with hypertension, diabetes, chronic kidney disease, dysphagia, and cognitive decline. An analysis of national dementia care guidelines showed that only the UK and Switzerland addressed assisted nutrition and hydration. "Jelly Drops," a hydrating product for dementia patients, received innovation awards. A study on US Physician Orders for Life Sustaining Treatment forms highlighted inconsistent terminology for end-of-life nutrition and hydration, calling for clearer language to aid decision-making., Summary: The new hydration-related recommendations in the ESPEN 2024 guidelines for dementia reflect a more individualized, proactive, and comprehensive approach to managing hydration. These guidelines emphasize the importance of early detection, personalized interventions, and consistent monitoring to ensure that dehydration is identified and treated promptly. Furthermore, the current literature supports the need for a specific terminology for dementia management using nutrition and hydration to improve patients' health outcomes., (Copyright © 2024 Wolters Kluwer Health, Inc. All rights reserved.)

Effective food safety monitoring requires a multi-step approach from farm to fork, involving different methods, ranging from convenient screening devices to sophisticated laboratory confirmatory testing. However, sample transportation to routine laboratories is time-consuming and expensive. Simplified on-site sampling followed by laboratory analysis offers a potential solution. Dried blood spot (DBS) cards ensure stability and ease of sample transportation and are used in clinical testing. However, the applicability of such an approach could be broader and include the storage of dried extract from more complex (solid) matrices. Therefore, a simplified approach is presented here, using DBS cards for on-site sampling and subsequent laboratory confirmation for food contaminants. To achieve this, an analytical tool (Smartcard) was designed using 3D-printing technology. As a proof of concept, the approach was applied to detect the pesticide fipronil, which is widely used in ornamental flower production to limit pests and on poultry farms. The Smartcard can securely store the sample extracts on a DBS card (dried extract spot (DExS) card), incorporate the lateral flow immunoassay (LFIA) and immediately provide an estimate of contamination levels. After simplified in-syringe extraction of the sample, the LFIA allows direct screening of fipronil (half maximum inhibitory concentration of 6.5 µg/l with calibration standards), and the same sample extract can be directly applied to the DExS card for storage and transport to the laboratory, where analyte re-extraction and instrumental analysis is performed using ultra high-pressure liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) detecting fipronil down to 0.8 µg/kg., Competing Interests: Declarations. Competing interests: The authors declare no competing interests., (© 2024. The Author(s).)