1. Differential infection dynamics in mononuclear and polymorphonuclear cells during Salmonella Typhimurium infection and in vitro exposure to diazoxon.
- Author
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Girón-Pérez DA, Ley-Arteaga LV, Covantes-Rosales CE, Toledo-Ibarra GA, Díaz-Resendiz KJG, Bueno-Durán AY, Benitez-Trinidad AB, Navidad-Murrieta MS, and Girón-Pérez MI
- Subjects
- Humans, Actins metabolism, Salmonella Infections microbiology, Cells, Cultured, Salmonella typhimurium drug effects, Reactive Oxygen Species metabolism, Leukocytes, Mononuclear drug effects, Neutrophils drug effects
- Abstract
S. Typhimurium bacteria cause one of the most recurrent gastrointestinal diseases worldwide. This bacterium can settle in the gastrointestinal tract and internalize into different cellular strains, causing the formation of cellular reservoirs that subsequently lead to systemic dissemination. Exogenous factors such as pesticide exposure can also cause immunological alterations, increasing susceptibility to bacterial infection. The present work evaluated the infection capacity of Salmonella Typhimurium, during a short period (1 h) on mononuclear and polymorphonuclear cells previously exposed to diazoxon (1 h, during 4 h). Mononuclear cells were infected more frequently and in greater magnitude than polymorphonuclear cells. However, when actin polymerization and the release of reactive oxygen species (ROS) were analyzed, polymorphonuclear cells showed increased activity. These processes were evidenced by conformational changes during infection. This suggests differential dynamics of S. Typhimurium infection in mononuclear and polymorphonuclear cells previously exposed to diazoxon., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2025 Elsevier Ltd. All rights reserved.)
- Published
- 2025
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