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Background: Humans and animals who have an acute case of diarrhea can be infected with Cryptosporidium spp. Within the category of water-borne disease, it is a zoonotic disease. The zoonotic disease Cryptosporidium is among the several pathogens carried by wild rats (Rattus spp.). The risk of spreading this disease is rather significant in urban environments because rats are often close to people. Aim: This study aims to detect Cryptosporidium spp. infection in wild rats in Surabaya, East Java, Indonesia. Methods: Through necropsy, a total of 100 wild rats' intestines were sampled for feces. Microscopic observation of the presence of Cryptosporidium was carried out using the float test with a combination of Ziehl Neelsen staining. Molecular detection of Cryptosporidium spp. positive results used the Cryptosporidium oocyst wall protein (COWP) gene with Polimerase Chain Reaction (PCR) method. Results: The results showed that 69 samples were positive for containing Cryptosporidium spp. oocysts and with Ziehl-Neelsen (ZN) staining to confirm the diagnosis, the staining results showed Cryptosporidium spp. oocysts dark pink with a clear cavity inside with a percentage of 95.65% in Rattus norvegicus and 61.03% in Rattus tanezumi. In residential and densely populated environments the percentage of Cryptosporidium spp. amounted to 66.66% and in the market environment amounted to 74.19%. Percentage of Cryptosporidium spp. in the North Surabaya region it was 42.85%, South Surabaya 100%, West Surabaya 37.5%, East Surabaya 81.39%, and Central Surabaya 65.38%. Molecular detection of Cryptosporidium spp. positive results were obtained using the Cryptosporidium oocyst wall protein (COWP) gene 550 bp. Conclusion: The high number of cases of cryptosporidiosis in wild rats This study aims to detect Cryptosporidium spp. infection in wild rats in Surabaya, East Java, Indonesia. has the potential to be a reservoir for the spread of the disease. The Cryptosporidium spp can detected with oocyst wall protein (COWP) in 550 bp in wild rats in Surabaya, East Java, Indonesia. [Open Vet J 2024; 14(10.000): 2687-2692]

Strategic infectious animal diseases have a great impact on the national economy as they may cause great economic loss, public anxiety, and high mortality. In Indonesia, among the diseases considered as strategic infectious animal diseases is foot and mouth disease (FMD). This study reported the incidence of strategic infectious animal diseases in Balongpanggang district during 2023. Throughout 2023, FMD only accounted for 4% of the total diseases of the year and was the only strategic infectious animal disease reported that year. Meanwhile, bovine ephemeral fever (BEF) incidence reached 57% (876/1546) of the total diseases in beef cattle in 2023. In addition to BEF, official veterinary services reported a total case of 1546 throughout 2023 that comprises; diarrhea (99), limping (24), helminthiasis (91), ectoparasite (11), hematochezia (3), wound (3), mastitis (4), allergy (1), dystocia (28), vaginal prolapse (11), dermatitis (45), enteritis (27), tympani (11), scabiosis (122), arthritis (67), ophthalmic problem (11), endometritis (1), uterine retention (13), indigestion (2), pneumonia (21), hypoglycemia (5), abscess (21), ovariohysterectomy (1), orchitis (1), intoxication (1), and papilloma (1). In conclusion, the only strategic infectious animal disease reported in 2023 in Balongpanggang was FMD with the incidence as high as 3% as compared to the non-strategic infectious animal diseases, BEF, which reached 54% of the total cases during 2023.

Background and Aim: Toxoplasmosis is caused by the parasite Toxoplasma gondii. Cats are the only known hosts that excrete resistant oocysts. Wild rats serve as crucial reservoirs and intermediate hosts for T. gondii’s survival and dissemination. Consuming soil and water containing oocysts can lead to illness. This study aimed to estimate the prevalence of toxoplasmosis in wild rats through molecular detection as an indicator of environmental contamination in Surabaya. Materials and Methods: One hundred rats were collected from the three areas (housing, dense settlements, and traditional markets) and distributed into the five zones: West, East, Central, North, and South of Surabaya. Brain tissue samples were extracted using a Geneaid™ (New Taipei City, Taiwan) DNA isolation kit and analyzed through the loop-mediated isothermal amplification (LAMP) method. Results: The study analyzed brain tissue from 100 wild rats, consisting of 77 Rattus tanezumi and 33 Rattus norvegicus, displaying 30% LAMP positivity. The study revealed that 30% (30/100) of wild rats tested were infected with T. gondii. The molecular prevalence rate in male rats was 32.35% (22/68), compared to females with 25% (8/32). 41.9% of the housing population, 33.3% of traditional markets, and 22.6% of dense settlements had the highest molecular prevalence. The high positive molecular rate at the trapping site can be attributed to cats and dense populations. Conclusion: Thirty percentage wild rats were tested positive for toxoplasmosis in Surabaya, East Java, Indonesia using LAMP method. Implementing strict control and monitoring is crucial in preventing the transmission of diseases from wild rats to humans. It is necessary to carry out further research related to genetic analysis of T. gondii to determine the type of T. gondii that infects animals and humans in Surabaya through bioassay and molecular test.

Cryptosporidiosis is a waterborne zoonosis caused by Cryptosporidium, which is a parasite that causes infection of the small intestine and leads to acute diarrhea in both humans and animals. Wild rats (Rattus spp.) are known to carry many zoonotic pathogens including Cryptosporidium. Given their close proximity to humans in urban environments, the likelihood of disease transmission is high. This study aims to detect the Cryptosporidium spp. infection in wild rats in Surabaya City, East Java Province. Intestinal fecal samples were collected using necropsy. The flotation test and Ziehl-Neelsen stain were used to observe the presence of Cryptosporidium under the microscope. The results showed that 69 samples were positive for Cryptosporidium spp. in the oocyst stage, which appeared dark pink with a clear cavity and the size of the oocysts ranged from 2-6 μm. The high incidence of cryptosporidiosis in wild rats is a potential health threat to both animals and humans.

Sex identification of Sun Conures (Aratinga solstitialis) is crucial for breeding and preservation, as well as increasing sun conure populations. These birds are sexually monomorphic. Therefore, Determination between male and female carried out by their morphology examination. The Polymerase Chain Reaction (PCR) method, utilizing molecular-based technology, was employed to determine the sex of Aratinga solstitialis in this study. The P2 and P8 primers were utilized in this method, which has been deemed suitable and accurate for sex identification through calamus samples. The research focused on two 28-month-old Aratinga solstitialis birds. Calamus samples were collected and subjected to PCR amplification using the extracted calamus. The resulting PCR products were then visualized using electrophoresis with a 1% agarose gel. In the electrophoresis photo, the presence of two bands indicated a female specimen, whereas a single band indicated a male specimen. The result of the gel electrophoresis research showed that both of the Aratinga solstitialis were male with one band of each bird on ranged from 300-400 base pairs. The result show that the Polymerase Chain Reaction method in terms for sex identification on monomorphic birds, especially Aratinga solstitialis birds is very effective to differentiate the sex of young birds and the adults.

Hepatic hydrothorax is a pleural effusion (typically ≥500 mL) that develops in patients with cirrhosis and/or portal hypertension in the absence of other causes. In most cases, hepatic hydrothorax is seen in patients with ascites. However, ascites is not always found at diagnosis and is not clinically detected in 20% of patients with hepatic hydrothorax. Some patients have no symptoms and incidental findings on radiologic examination lead to the diagnosis of the condition. In the majority of cases, the patients present with symptoms such as dyspnea at rest, cough, nausea, and pleuritic chest pain. The diagnosis of hepatic hydrothorax is based on clinical manifestations, radiological features, and thoracocentesis to exclude other etiologies such as infection (parapneumonic effusion, tuberculosis), malignancy (lymphoma, adenocarcinoma) and chylothorax. The management strategy involves a stepwise approach of one or more of the following: Reducing ascitic fluid production, preventing fluid transfer to the pleural space, fluid drainage from the pleural cavity, pleurodesis (obliteration of the pleural cavity), and liver transplantation. The complications of hepatic hydrothorax are associated with significant morbidity and mortality. The complication that causes the highest morbidity and mortality is spontaneous bacterial empyema (also called spontaneous bacterial pleuritis).