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Microfiltration (MF) membrane performance in separation of red ginger (Zingiber officinale) through dead-end stirred filtration cell (DESFC) was conducted to concentrate with the best total polyphenol as natural antioxidant. Separation process of fermented red ginger of A and fermented red ginger of B were performed through MF membrane (pores size of 0.15 µm) fitted in DESFC at fixed trans-membrane pressure (TMP) of 20 psia, and stirrer rotation speed of 200, 300 and 400 rpm for 30 minutes. Fermented red ginger of A and fermented red ginger of B were the result of fermentation of red ginger with concentrations of red ginger extracts of 5 and 10% (v/v) by kombucha inoculum of 20% for 14 days at room temperature. The result of research showed that based on optimization of flux, the best DESFC performance on fermented red ginger of A and fermented red ginger of B were obtained at stirrer rotation speed of 400 rpm and TMP of 20 psia and gave fluxes of 0.0418 and 0.0342 mL/cm2.min. At these condition of optimum flux were generated retentate of fermented red ginger of A and fermented red ginger of B with compositions of total polyphenol of 0.999 and 1.024%, total solids of 14.02 and 11.43 %, and inhibition of 89.82 and 89.57%, while permeates of fermented red ginger of A and fermented red ginger of B had compositions of total polyphenol of 0.929 and 0.527%, total solids of 12.75 and 10.68%, and inhibition of 91.25 and 90.24%. In these conditions of optimum fluxes, fermented red ginger of A and fermented red ginger of B showed mass spectra with polyphenol monomer dominated by Acetoxy-[8]-gingerol with molecular weight (MW) of 365.266 Dalton (Da.) and Diacetoxy-[6] gingerdiol with MW of 381.206 Da., and relative intensity of 100% and 37.63%, respectively. [ABSTRACT FROM AUTHOR]