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As a developing country with an agricultural economy as a pillar, Bangladesh is highly vulnerable to adverse effects of climate change, so the generation of high-resolution temperature maps is of great value for Bangladesh to achieve agricultural sustainable development. However, Bangladesh's weak economy and sparse meteorological stations make it difficult to obtain such maps. In this study, by mining internal features and links inside observed data, we developed an efficient data-driven downscaling technique to generate high spatial-resolution temperature distribution maps of Bangladesh directly from observed temperature data at 34 meteorological stations with irregular distribution. Based on these high-resolution historical temperature maps, we further explored a data-driven forecast technique to generate high-resolution temperature maps of Bangladesh for the period 2025–2035. Since the proposed techniques are very low-cost and fully mine internal links inside irregular-distributed observations, they can support relevant departments of Bangladesh to formulate policies to mitigate and adapt to climate change in a timely manner. [ABSTRACT FROM AUTHOR]

Metallothionein (MTs) can be used in the prevention and treatment of tumors and diabetes due to its antioxidant properties. However, it is necessary to solve its non-transmembrane properties and further improve its antioxidant activity, increase its fluorescence visualization and enhance its stability to meet practical applications in the biomedical field. Here, we report the preparation of a novel metallothionein-AuNP composite material with high transmembrane ability, fluorescence visualization, antioxidant activity, and stability by genetic modification (introducing transduction peptide TAT, fluorescence tag GFP and increasing sulfydryl groups) and immobilization technology (covalently bonding with AuNPs). The transmembrane activity of modified proteins was verified by immunofluorescence. Increasing the sulfhydryl content within a certain range can enhance the antioxidant activity of the protein. In addition, GFP were used to further simplify the imaging of the metallothionein-AuNP composite in cells. XPS results indicated that AuNPs can immobilize metallothionein through Au S covalent bonds. TGA characterization and degradation experiments showed that thermal and degradation stability of the immobilized material was significantly improved. This work provides new ideas to construct metallothionein composites with high transmembrane ability, antioxidant activity, fluorescence visualization and stability to meet novel applications in the biomedical field. [Display omitted] • TAT- Sh MT3 was obtained by long primer PCR. • TAT- Sh MT3 has about 30 % increase in antioxidant capacity compared to TAT- Sh MT. • TAT- Sh MT3-GFP was obtained by SOE-PCR. • TAT- Sh MT3-GFP + AuNPs was obtained Au S covalent bond. • TAT-ShMT3-GFP + AuNPs has higher stability compared to TAT- Sh MT3-GFP. [ABSTRACT FROM AUTHOR]

Nurudea zhengii Ren was identified by aphid morphological characteristics as well as the gall shape and host plant species, and placed in the tribe Fordini (Hemiptera, Aphididae, Eriosomatinae). Here, its whole genome was firstly sequenced by a genome-skimming method and its mitochondrial genome (mitogenome) was assembled to examine its genetic variation and phylogenetic position. The complete mitogenome of Nurudea zhengii is 15,392 bp in length, and consists of 13 protein-coding genes, 22 tRNAs, two rRNAs and one D-loop region. The gene order follows the mitogenomes of the other Rhus gall aphids, and similarly has an AT bias with the content of 83.9%. The majority strand is A-skewed and C-skewed, and shows opposite skewness for G-skewed in the minority strands. The ratios of nonsynonymous to synonymous substitution rates of protein-coding genes are lower than one except for ATP8, which indicated that ATP8 was undergoing positive selection. Phylogenetic analysis among the Rhus gall aphids based on 13 protein-coding genes and two rRNA genes showed that N. zhengii was sister to N. shiraii, and then clustered with N. yanoniella as a group with high support value. The two species, N. shiraii and N. yanoniella, share the same host plant Rhus chinensis, while the host of N. zhengii is R. hypoleuca. However, the phylogenetic relationship indicated that the taxa sharing the same host plant were not absolutely clustered as the closest taxa at least at species level. [ABSTRACT FROM AUTHOR]