1. Recent mechanistic developments for cytochrome c nitrite reductase, the key enzyme in the dissimilatory nitrate reduction to ammonium pathway.
- Author
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Hird K, Campeciño JO, Lehnert N, and Hegg EL
- Subjects
- Cytochromes c1 metabolism, Cytochromes c1 chemistry, Nitrate Reductases metabolism, Nitrate Reductases chemistry, Catalytic Domain, Electron Transport, Nitrites metabolism, Cytochromes a1, Nitrates metabolism, Nitrates chemistry, Oxidation-Reduction, Ammonium Compounds metabolism
- Abstract
Cytochrome c nitrite reductase, NrfA, is a soluble, periplasmic pentaheme cytochrome responsible for the reduction of nitrite to ammonium in the Dissimilatory Nitrate Reduction to Ammonium (DNRA) pathway, a vital reaction in the global nitrogen cycle. NrfA catalyzes this six-electron and eight-proton reduction of nitrite at a single active site with the help of its quinol oxidase partners. In this review, we summarize the latest progress in elucidating the reaction mechanism of ammonia production, including new findings about the active site architecture of NrfA, as well as recent results that elucidate electron transfer and storage in the pentaheme scaffold of this enzyme., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)
- Published
- 2024
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