1. A dual-signal output electrochemical aptasensor for glypican-3 ultrasensitive detection based on reduced graphene oxide-cuprous oxide nanozyme catalytic amplification strategy.
- Author
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Li G, Feng H, Li X, Li S, Liang J, and Zhou Z
- Subjects
- Humans, Aptamers, Nucleotide chemistry, Catalysis, Oxidation-Reduction, Hydrogen Peroxide chemistry, Graphite chemistry, Glypicans blood, Glypicans analysis, Electrochemical Techniques methods, Limit of Detection, Biosensing Techniques methods, Copper chemistry
- Abstract
Glypican-3 (GPC3) is an essential reference target for hepatocellular carcinoma detection, follow-up and prediction. Herein, a dual-signal electrochemical aptasensor based on reduced graphene oxide-cuprous oxide (RGO-Cu
2 O) nanozyme was developed for GPC3 detection. The RGO-Cu2 O nanoenzyme displayed excellent electron transport effect, large specific surface area and outstanding peroxidase-like ability. The differential pulse voltammetry (DPV) signal of Cu2 O oxidation fraction and the chronoamperometry (i-t) signal of H2 O2 decomposition catalyzed by RGO-Cu2 O nanozyme were used as dual-signal detection. Under optimal conditions, the log-linear response ranges were 0.1 to 500.0 ng/mL with the limit of detection 0.064 ng/mL for DPV technique, and 0.1-50.0 ng/mL for i-t technique (detection limit of 0.0177 ng/mL). The electrochemical aptasensor has remarkably analytical performance with wide response range, low detection limit, excellent repeatability and specificity, good recovery in human serum samples. The two output signals of one sample achieve self-calibration of the results, effectively avoiding the occurrence of possible leakage and misdiagnosis of a single detection signal, suggesting that it will be a promising method in the early biomarker detection., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)- Published
- 2024
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