Search

Objective·To investigate the effects of trimethylamine oxide (TMAO) on cardiac function in mice with hypertrophic cardiomyopathy (HCM) and its potential molecular mechanism.Methods·Mice with myosin heavy chain 6 (Myh6) c.1211G>A (p.R404Q+/-) point mutation were used as the animal model. According to dietary supplementation of TMAO and TMAO inhibitor iodomethylcholine (IMC), the wild type (WT) mice and HCM mice were divided into WT group, HCM group (HCM-1 group, HCM-2 group), WT+TMAO group, HCM+TMAO group and HCM+IMC group, respectively. Left ventricular fraction shortening (FS) and left ventricular posterior wall thickness (LVPW) were assessed by echocardiography in all mice. Enzyme-linked immunosorbent assay (ELISA) was used to detect the serum TMAO concentration of mice in the HCM-1 group and WT group. The regularity of myocardial cell arrangement of mice was evaluated by hematoxylin and eosin staining (HE staining). The proportion of myocardial fibrosis was evaluated by Masson staining. The activity of protein kinase A (PKA) in mouse myocardial tissue was detected by PKA kit. The expression of ryanodine receptor 2 (RyR2) and p-RyR2(S2808) in mouse myocardial tissue was detected by Western blotting.Results·The results of echocardiography showed that at 12 months of age, the FS of mice in the WT+TMAO group and HCM+TMAO group were lower than those in the corresponding WT group and HCM-1 group, respectively (P

How to effectively maintain costly intra-group cooperation has always been a topic of great concern in society. As we all know, inter-group competition is a constructive way to promote intra-group cooperation. Previous inter-group competition mechanisms are primarily based on the total contribution value of all individuals in the group in the current round, which ignore the historical behavior of the group and its members. For comparison, we argue that the introduction of collective reputation which reflects not only the total contribution of the groups in the current round but also the group's historical behavior is necessary for the construction of an inter-group competition model. Furthermore, an excellent collective reputation can help the group obtain more high-quality resources and help the group stand out from the competition. To this end, we introduce a new mechanism of inter-group transfer payment driven by collective reputation and construct a public goods game (PGG) model of inter-group competition to study the dynamic behavior of intra-group cooperation evolution. Two parameters, the proportion of accepted groups a and the proportion of transferred groups b , are introduced. The simulation results suggest that the effectiveness of this model in promoting cooperation depends largely on the joint settings of a and b. And in most cases, the combination of small a and medium b plays the significant role. [ABSTRACT FROM AUTHOR]

Robotic surgery is known as the "third technological revolution" in the field of surgery, and is an important milestone in the development of modern surgery. However, our country's innovative surgical robot industry is still in its early stages, and it is only being utilized in certain surgical fields. To explore the effectiveness of the application of domestic surgical robot in oral and maxillofacial surgery, the author successfully completed a case of benign parotid tumor resection with the assistance of a domestic autonomous robot. The operation was successful, facial nerve function was preserved, and postoperative wound healing was good.