【Objective】This study is aimed to construct a konjac cDNA library and screen for interacting proteins of the transcription factor WRKY72 in order to elucidate the molecular mechanism underlying WRKY72-mediated konjac resistance against diseases.【Method】Konjac plants at different stages of bacterial soft rot infection were used as experimental materials. Total RNA was extracted, pooled in equal amounts, and used to construct a konjac cDNA library. The bait vector containing the transcription factor WRKY72 was constructed using the homologous recombination method, and a yeast two-hybrid screen was performed to identify candidate target proteins, which were further verified individually.【Result】The yeast library titer was 1.6×107 CFU/mL, and the average length of the inserted fragments was around 1 000 bp. The bait vector was verified to have no self-activation in yeast. The bait vector pGBKT7-WRKY72 was used to screen library and 41 positive interacting proteins were obtained, including transport proteins, channel proteins, heat shock proteins, chlorophyll-binding proteins, and peroxidases. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analyses were performed on these interacting proteins, and 12 of them were further verified by one-on-one yeast two-hybrid assays.【Conclusion】A high-quality konjac cDNA library was successfully constructed, and the interacting proteins of the transcription factor WRKY72 were screened, providing a theoretical basis for elucidating the mechanism of resistance against soft rot disease in konjac. [ABSTRACT FROM AUTHOR]