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Abstract. Background:. We previously reported that activation of the cell cycle in human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) enhances their remuscularization capacity after human cardiac muscle patch transplantation in infarcted mouse hearts. Herein, we sought to identify the effect of magnesium lithospermate B (MLB) on hiPSC-CMs during myocardial repair using a myocardial infarction (MI) mouse model. Methods:. In C57BL/6 mice, MI was surgically induced by ligating the left anterior descending coronary artery. The mice were randomly divided into five groups (n = 10 per group); a MI group (treated with phosphate-buffered saline only), a hiPSC-CMs group, a MLB group, a hiPSC-CMs + MLB group, and a Sham operation group. Cardiac function and MLB therapeutic efficacy were evaluated by echocardiography and histochemical staining 4 weeks after surgery. To identify the associated mechanism, nuclear factor (NF)-κB p65 and intercellular cell adhesion molecule-1 (ICAM1) signals, cell adhesion ability, generation of reactive oxygen species, and rates of apoptosis were detected in human umbilical vein endothelial cells (HUVECs) and hiPSC-CMs. Results:. After 4 weeks of transplantation, the number of cells that engrafted in the hiPSC-CMs + MLB group was about five times higher than those in the hiPSC-CMs group. Additionally, MLB treatment significantly reduced tohoku hospital pediatrics-1 (THP-1) cell adhesion, ICAM1 expression, NF-κB nuclear translocation, reactive oxygen species production, NF-κB p65 phosphorylation, and cell apoptosis in HUVECs cultured under hypoxia. Similarly, treatment with MLB significantly inhibited the apoptosis of hiPSC-CMs via enhancing signal transducer and activator of transcription 3 (STAT3) phosphorylation and B-cell lymphoma-2 (BCL2) expression, promoting STAT3 nuclear translocation, and downregulating BCL2-Associated X, dual specificity phosphatase 2 (DUSP2), and cleaved-caspase-3 expression under hypoxia. Furthermore, MLB significantly suppressed the production of malondialdehyde and lactate dehydrogenase and the reduction in glutathione content induced by hypoxia in both HUVECs and hiPSC-CMs in vitro. Conclusions:. MLB significantly enhanced the potential of hiPSC-CMs in repairing injured myocardium by improving endothelial cell function via the NF-κB/ICAM1 pathway and inhibiting hiPSC-CMs apoptosis via the DUSP2/STAT3 pathway.

The influence of thermal damage on macroscopic and microscopic characteristics of different rocks has received much attention in the field of rock engineering. When the rocks are subjected to thermal treatment, the change of macroscopic characteristics and evolution of micro-structure would be induced, ultimately resulting in different degrees of thermal damage in rocks. To better understand the thermal damage mechanism of different rocks and its effect on the rock performance, this study reviews a large number of test results of rock specimens experiencing heating and cooling treatment in the laboratory. Firstly, the variations of macroscopic behaviors, including physical parameters, mechanical parameters, thermal conductivity and permeability, are examined. The variations of mechanical parameters with thermal treatment variables (i.e. temperature or the number of thermal cycles) are divided into four types. Secondly, several measuring methods for microstructure, such as polarizing microscopy, fluorescent method, scanning electron microscopy (SEM), X-ray computerized tomography (CT), acoustic emission (AE) and ultrasonic technique, are introduced. Furthermore, the effect of thermal damage on the mechanical parameters of rocks in response to different thermal treatments, involving temperature magnitude, cooling method and thermal cycle, are discussed. Finally, the limitations and prospects for the research of rock thermal damage are proposed.

Abstract Background Long-chain acyl-coenzyme A synthetase (LACS) is a type of acylating enzyme with AMP-binding, playing an important role in the growth, development, and stress response processes of plants. Results The research team identified different numbers of LACS in four cotton species (Gossypium hirsutum, Gossypium barbadense, Gossypium raimondii, and Gossypium arboreum). By analyzing the structure and evolutionary characteristics of the LACS, the GhLACS were divided into six subgroups, and a chromosome distribution map of the family members was drawn, providing a basis for further research classification and positioning. Promoter cis-acting element analysis showed that most GhLACS contain plant hormones (GA, MeJA) or non-biological stress-related cis-elements. The expression patterns of GhLACS under salt stress treatment were analyzed, and the results showed that GhLACS may significantly participate in salt stress response through different mechanisms. The research team selected 12 GhLACSs responsive to salt stress for tissue expression analysis and found that these genes are expressed in different tissues. Conclusions There is a certain diversity of LACS among different cotton species. Analysis of promoter cis-acting elements suggests that GhLACS may be involved in regulating plant growth, development and stress response processes. GhLACS25 was selected for in-depth study, which confirmed its significant role in salt stress response through virus-induced gene silencing (VIGS) and induced expression in yeast cells.

Abstract Background Vasculopathy is the most common complication of diabetes. Endothelial cells located in the innermost layer of blood vessels are constantly affected by blood flow or vascular components; thus, their mechanosensitivity plays an important role in mediating vascular regulation. Endothelial damage, one of the main causes of hyperglycemic vascular complications, has been extensively studied. However, the role of mechanosensitive signaling in hyperglycemic endothelial damage remains unclear. Methods Vascular endothelial-specific Piezo1 knockout mice were generated to investigate the effects of Piezo1 on Streptozotocin-induced hyperglycemia and vascular endothelial injury. In vitro activation or knockdown of Piezo1 was performed to evaluate the effects on the proliferation, migration, and tubular function of human umbilical vein endothelial cells in high glucose. Reactive oxygen species production, mitochondrial membrane potential alternations, and oxidative stress-related products were used to assess the extent of oxidative stress damage caused by Piezo1 activation. Results Our study found that in VECreERT2;Piezo1flox/flox mice with Piezo1 conditional knockout in vascular endothelial cells, Piezo1 deficiency alleviated streptozotocin-induced hyperglycemia with reduced apoptosis and abscission of thoracic aortic endothelial cells, and decreased the inflammatory response of aortic tissue caused by high glucose. Moreover, the knockout of Piezo1 showed a thinner thoracic aortic wall, reduced tunica media damage, and increased endothelial nitric oxide synthase expression in transgenic mice, indicating the relief of endothelial damage caused by hyperglycemia. We also showed that Piezo1 activation aggravated oxidative stress injury and resulted in severe dysfunction through the Ca2+-induced CaMKII-Nrf2 axis in human umbilical vein endothelial cells. In Piezo1 conditional knockout mice, Piezo1 deficiency partially restored superoxide dismutase activity and reduced malondialdehyde content in the thoracic aorta. Mechanistically, Piezo1 deficiency decreased CaMKII phosphorylation and restored the expression of Nrf2 and its downstream molecules HO-1 and NQO1. Conclusion In summary, our study revealed that Piezo1 is involved in high glucose-induced oxidative stress injury and aggravated endothelial dysfunction, which have great significance for alleviating endothelial damage caused by hyperglycemia.

20CrMnTi steel is widely used in the gear manufacturing field of engineering machinery. The addition of Ti element to steel can effectively improve its low-temperature impact toughness and enhance its mechanical properties. However, during the smelting process of 20CrMnTi, large-sized TiN inclusions are inevitably formed in the steel, which affects its service performance improvement. This paper focuses on the directional modification behaviors of TiN inclusions in 20CrMnTi gear steel by rare earth Ce under deoxygenation conditions of Si–Ca–Ba alloy. The results show that under the deoxygenation conditions of Si–Ca–Ba alone, there are composite inclusions in 20CrMnTi steel with CaAl12O19 as the core and TiN wrapped around the periphery, all of which have sizes greater than 2 μm. This type of inclusion is square or diamond shaped, and the addition of Si–Ca–Ba deoxygenation alone has poor TiN modification effect on gear steel 20CrMnTi. Adding Ce element can effectively improve the nucleation core of inclusions. The CeAlO3–TiN and (CaAl12O19–Ba–Ce)–TiN composite inclusions are formed, and the TiN inclusions are modified in terms of morphology and size. The average size of inclusions decreases from 4.1 to 3.0 μm, which prevented the excessive growth of pure TiN inclusions to some extent.

Abstract Metallurgical dust and sludge are solid waste resources with recycling value. In recent years, rotary hearth furnace has become the most important means to treat metallurgical dust and sludge because of its wide range of raw materials and strong treatment capacity. In this study blast furnace ash and converter sludge were selected as the research objects, and high-quality metallized pellets were prepared based on the rotary hearth furnace process. The strength changed of pellets, the reduction process of iron oxides and the removal process of zinc during the roasting of pellets in rotary hearth furnace were studied. To explore the reasonable roasting condition for preparing metallized pellets in rotary hearth furnace. The optimum roasting temperature of the pellets was 1250℃ and the roasting time was 25 min. The compressive strength, metallization rate and dezincification rate of metallized pellets reached 1361N, 97.44% and 95.67%, respectively. The efficient resource utilization of various metallurgical dust and sludge is realized.

The shale oil and gas exploration potential of the Jurassic Qianfoya Formation in the Sichuan Basin is promising. However, there is still a lack of understanding regarding the basic sedimentary characteristics and development patterns of fine-grained sedimentary rocks. Therefore, utilizing the principles of fine-grained sedimentology, this study thoroughly examines the geological features of the fine-grained sedimentary rock reservoir in the second member of the Qianfoya Formation in the Yuanba area. This investigation includes the analysis of material composition, sedimentary structures, reservoir space types, and classification of lithofacies types. Subsequently, the physical characteristics and reservoir space types of the main lithofacies reservoirs are compared and analyzed. Finally, the study predicts the favorable intervals for development. The results reveal that the fine-grained sedimentary rocks in the second member of the Qianfoya Formation in the Yuanba area consist of two rock types: clay rock and siltstone, with a total of 15 rock facies types. Organic-rich massive clay rock, organic-rich massive silty clay rock, organic-rich laminated silty clay rock, organic-rich banded silty clay rock, organic-rich massive silty clay rock, and organic-rich laminated silty clay rock are the six major lithofacies types of fine-grained sedimentary rock. Additionally, various types of reservoir spaces exist, such as interlayer fractures among clay minerals, shale bedding fractures, intercrystalline pores among clay minerals, intercrys-talline pores among iron pyrite, dissolved pores, and organic matter pores. Among these, intercrystalline pores of clay minerals are the most widely distributed. The average porosity and permeability of the six major lithofacies types were found to be 2% and 0.023×10-3 μm2, respectively. The lithofacies of organic-rich laminated silty clay rock, organic-rich laminated silty clay rock, and organic-rich massive silty clay rock exhibit rich reservoir space types, wide distribution, large quantity, higher porosity and permeability compared to other lithofacies, as well as higher TOC values, making them the most favorable reservoir lithofacies types. The dominant stratum of the second member of the Qianfoya Formation in the study area is situated in substratum 1, the bottom of substratum 2, and the middle and lower parts of substratum 3.

20CrMnTi gear steel has high low-temperature impact toughness and is widely used in the manufacturing of engineering machinery such as automotive transmission gears. The sizes and distributions of TiN inclusions have a significant impact on the service performances of 20CrMnTi gear steel. Controlling the growth behaviors and size distributions of TiN inclusions are the foundation for stable, high-quality, and efficient production of 20CrMnTi gear steel. This paper uses experimental and computational methods to study the effects of different ratios of Al–Ce composite deoxidizers on the modification behaviors of TiN inclusions in 20CrMnTi steel. The results show that the TiN inclusions in 20CrMnTi steel without Al–Ce composite deoxidizer are square in shape, with a size of approximately 4 μm. After Al–Ce composite deoxidation, a composite phase inclusion is formed in the steel, which has contracted edges and corners. The center is the nucleation core composed of elements such as Ce, Al, O, etc. The elements of Ti and N are wrapped around the periphery of the composite inclusion, reducing its size to 2 μm. When the ratio of Al and Ce in the Al–Ce composite deoxidizer is 7:3, the number of TiN inclusions in steel is the highest, accounting for 51.7% of the total amount of titanium inclusions. The average diameter of inclusions in the modified steel decreases to 2.4 μm. When the ratio of Al and Ce elements is 8:2 and 7:3, the final deoxygenation product is CeAlO3, which is more conducive to the modification of TiN inclusions in 20CrMnTi steel.

Precise positioning is a never‐ending goal in both fundamental science and technology. Recent decades of advancements in high‐precision position detection have predominantly relied on photoelectric effects for light detection in semiconductors. Herein, a different approach is proposed: The thermoelectric‐based position‐sensitive detector (T‐PSD) concept is designed to detect single heat spots arising from various energy sources, including electromagnetic radiation, electrons, and macroscopic mechanical heat. The T‐PSD concept is initially derived mathematically from the fundamental principles of heat conduction and the Seebeck effect. Subsequently, it is proved by finite element simulation in both 1D and 2D configurations. Following this theoretical groundwork, T‐PSD prototypes are fabricated and subjected to positional detection using various stimuli such as CO2 laser beam, hot soldering tip, and electron beam. In the prototypes, structured aluminum‐doped zinc oxide thermoelectric thin films, prepared via atomic layer deposition, are outfitted with voltage probes, enabling the measurement of thermoelectric voltages as a function of position and the intensity or temperature of the heat spot. Furthermore, practical decoding strategies are introduced to infer the position from the measured signals. The T‐PSD in this article showcases considerable promise in high‐precision position detection such as (quasi‐)particle tracking and precision machinery, offering an alternative concept in PSD design.

The world has been experiencing huge upheavals since the COVID-19 crises. Conflicts caused by opposing ideologies, economic-political agendas and realities are rampant within or across borders. Such unsteady circumstances contribute to shifting how Transnational Education (TNE) occurs worldwide, as well as the scientific, epistemic and educational discourses that go with it. Anchored within critical interculturality, this paper explores the concept of ‘inter-ideologicality’. The study looks at short-term online international student mobility to demonstrate how students from China and Finland navigate and negotiate ideologies around the concept of culture in intercultural research and education. The study also employs Wang Chong’s perspectives on criticality to identify emerging ideologies in the co-construction of criticality in students’ online cooperation. Findings reveal that (1) two ideological orientations, nation-oriented and society-oriented, developed during discussions about culture; (2) Finnish students used a specific form of reasoning to contradict Chinese students’ thoughts about Finland by means of criticality towards the nation-oriented ideology; (3) Chinese and Finnish students employed questioning and challenging to help each other be aware of something left unsaid about the status of women in their societies within the society-oriented ideology. The study represents an important meta-approach to intercultural communication education within internationalization of Higher Education, aimed at supporting mobile students to reflect critically on the scientific and educational notion of interculturality rather than providing them with ready-made recipes as to how to communicate and behave interculturally.

Abstract Mollisols are highly fertile soils and function as significant carbon reservoirs. However, determining their ages and formation processes is challenging due to extensive pedoturbation, which undermines conventional dating methods. Here, we employed luminescence, a light‐sensitive property of minerals widely used in geological dating, to investigate and quantify soil mixing. We analyzed over 2,400 luminescence ages of individual K‐feldspar grains from a Mollisol profile in Northeast China, and for the first time, we were able to determine the intensity of pedoturbation in the Mollisol profile over the past 50,000 years. The results showed that the current pedoturbation can penetrate to a depth of approximately 80 cm, with the intensity decreasing with depth. By identifying a significant intensification in historical pedoturbation, we inferred that the paleoenvironment might be suitable for the formation of Mollisols 16,400 years before present.

Summary: Previous studies have indicated the neuroprotective effect of olfactory mucosa mesenchymal stem cells (OM-MSCs) on brain injury. Intracerebral hemorrhage (ICH) models were established in rats by injecting autologous blood. SENP1 expression was enhanced in neurons but decreased in astrocytes compared to that in OM-MSCs. Overexpression of SENP1 promoted the proliferation and neuronal differentiation, while inhibiting the astrocytic differentiation of OM-MSCs. Conversely, its knockdown had the opposite effect. Moreover, OM-MSCs reduced neurological dysfunction in rats after ICH, and the neuroprotective effect of OM-MSCs could be further enhanced by SENP1 overexpression. In addition, SENP1 promoted mitophagy, which might be related to SENP1-mediated OPTN deSUMOylation. Furthermore, SENP1 promoted neuronal differentiation of OM-MSCs through mitophagy mediated by OPTN. Similar to SENP1, OPTN transfection further enhanced the remission effect of OM-MSC on ICH rats. SENP1 promoted neuronal differentiation of OM-MSCs through OPTN-mediated mitophagy to improve neurological deficits in ICH rats.

Abstract Purpose This study was aimed to identify the risk factors that influence the mortality risk in patients with acute aortic dissection (AAD) within one year after discharge, and aimed to construct a predictive model for assessing mortality risk. Methods The study involved 320 adult patients obtained from the Medical Information Mart for Intensive Care (MIMIC) database. Logistic regression analysis was conducted to identify potential risk factors associated with mortality in AAD patients within one year after discharge and to develop a predictive model. The performance of the predictive model was assessed using the receiver operating characteristic curve (ROC), calibration curve, and decision curve analysis (DCA). To further validate the findings, patient data from the First Affiliated Hospital of Guangxi Medical University (157 patients) were analyzed. Results Univariate and multivariate logistic regression analyses revealed that gender, length of hospital stay, highest blood urea nitrogen (BUN_max), use of adrenaline, and use of amiodarone were significant risk factors for mortality within one year after discharge (p

Objective: The aim of this study was to explore the effects of Babao dan (BBD), a traditional Chinese medicine, on gastric cancer (GC) progression in vivo. Materials and Methods: A subcutaneous xenograft mouse model of GC was established using MGC80-3 cells. The terminal deoxynucleotidyl transferase-mediated dUTP: 2'-deoxyuridine 5'-triphosphate -biotin nick-end labeling method was adopted to detect cell apoptosis in vivo. The expression levels of proteins associated with proliferation, apoptosis, and angiogenesis were measured by immunohistochemical staining or western blotting (WB). The activation and protein levels of p-c-Jun N-terminal kinase (JNK), p-p38, p-extracellular-regulated kinase 1/2, p-nuclear factor-κB (NF-κB), and p-STAT3 were examined by Bio-plex and WB. Results: BBD significantly inhibited tumor growth in GC mouse models with no adverse effect on body weight or organ function. It was also found that BBD significantly suppressed the proliferation of GC tumor cells, induced the apoptosis of tumor cells, and inhibited angiogenesis through inactivating with mitogen-activated protein kinase, NF-κB, and STAT3 pathways. Conclusions: BBD exerts suppressive effects on GC tumor growth by regulating multiple pathways in vivo, which may provide a novel treatment option for GC therapy.

Chlorophyll-a is an important parameter that is used to measure the water quality, and its concentrations indicate the degree of eutrophication. Taking the waters of Chaohu Lake as the research area, four remote sensing inversion models of the chlorophyll-a concentrations were constructed based on GF-1 WFV images using empirical and semiempirical/semianalytical methods. The optimal model was selected by evaluating the model validation accuracies, and this model was used to invert the spatial distributions of the chlorophyll-a concentrations in the waters of Chaohu Lake from May to July 2017. The results show that the NDCI model is the most suitable for inverting chlorophyll-a concentrations in the waters of Chaohu Lake based on GF-1 WFV images. This model has the highest validation accuracy, with a determination coefficient of 0.933, a Root mean square error of $2.85 ~\mu \text{g}$ /L and a mean absolute percentage error of 19.69%. The spatial distributions of the chlorophyll-a concentrations in the waters of Chaohu Lake from May to July 2017 all showed decreasing trends from Western Half Lake to Eastern Half Lake, the areas of higher chlorophyll-a concentrations in May and June were mainly concentrated in the northern waters of Western Half Lake, and the area with the highest chlorophyll-a concentrations in July was located in the northwestern waters of Middle Half Lake. The mean chlorophyll-a concentration in the waters of Chaohu Lake from May to July 2017 was $12.5 ~\mu \text{g}$ /L, which indicated slight eutrophication.

In light of the steel industry's rapid advancements, the availability of high-quality mineral resources is diminishing. Therefore, the recovery of iron from BOF slag is of great significance to the sustainability development. Considering the compositional characteristics of BOF slag, the transformation of the iron-containing phase into (Mn,Mg)yFe3-yO4 is the key step. Thus, a novel process for recovering iron resources by synergistic treatment of blast furnace slag (BFS) and BOF slag was proposed. This research employed FactSage thermodynamic simulation, XRD, SEM-EDS, XPS, and EPMA to analysis the impact of BFS addition (10–50 %), cooling methods (from water-cooling to furnace-cooling), and temperature (1400–1600 °C) on phase transformation and the RO oxidation mechanism, and the conditions of (Mn,Mg)yFe3-yO4 generation and enrichment was obtained. The results show that at BFS addition of 30 %, reaction temperature 1400 °C and furnace-cooling, the iron-containing phase (Ca2Fe2O5 and RO) was almost completely transformed into (Mn,Mg)yFe3-yO4. The oxidation mechanism of RO was formation of (Mn,Mg)yFe3-yO4 by cation diffusion. Under optimal conditions, the iron recovery rate and the grade reached 65.74 % and 32.07 %, respectively, which can be used as raw material for ironmaking. Meanwhile, the main phase of the tailing slag was β-Ca2SiO4, without f-CaO, which has the potential to be used in the cement and concrete industries with the advantages of both low cost and eco-friendly. Therefore, the process with green, efficient and low cost was provided, which is a feasible idea for the comprehensive utilization of industrial solid waste.

The cultivation of largemouth bass (Micropterus salmoides), a species of significant economic value in aquaculture, has experienced notable growth recently. However, the deterioration of water quality seriously affects the metabolic responses of M. salmoides. While compound microbial agent (CMA) is widely utilized for ecological rehabilitation and water filtration, its application in M. salmoides has not been reported. Here, based on physio-biochemical tests and 16S rRNA sequencing, we investigated the effects of CMA (yeast, Bacillus subtilis, and lactic acid bacteria) on the water quality within the recirculating aquaculture system, along with physiological indices and gut microbiota of M. salmoides. Compared to the control and single microbial agent (yeast), CMA treatment improved the water quality by improving the dissolved oxygen and delaying the increase of pH, total nitrogen, total phosphorus, ammonia nitrogen, and nitrite. The 16s rRNA gene sequencing revealed that the water treated with CMA exhibited elevated levels of chao1, Shannon, Pd, and a larger population of dominant bacterial. Besides, higher values of ACE, chao1, Shannon, and OTU level, and lower Simpson index were found in CMA treated M. salmoides samples, suggesting that CMA treatment enhanced the species richness and diversity of gut microbiota of M. salmoides. Furthermore, CMA treatment hindered the generation and proliferation of harmful bacteria, such as the Mycoplasma mobile 163K species and the Erysipelotrichaceae family, which was associated with enhanced antioxidant enzymatic activity and decreased MDA level in both the serum and liver. These findings shed light on the essential roles of CMA in M. salmoides culturing and introduce an innovative approach to enhance the aquatic environment.

Background: Hypertension, a highly prevalent chronic disease, is known to inflict severe damage upon blood vessels. In our previous study, isoliensinine, a kind of bibenzyl isoquinoline alkaloid which isolated from a TCM named Lotus Plumule (Nelumbo nucifera Gaertn), exhibits antihypertensive and vascular smooth muscle proliferation-inhibiting effects, but its application is limited due to poor water solubility and low bioavailability. In this study, we proposed to prepare isoliensinine loaded by PEG-PLGA polymer nanoparticles to increase its efficacy Method: We synthesized and thoroughly characterized PEG-PLGA nanoparticles loaded with isoliensinine using a nanoprecipitation method, denoted as, PEG-PLGA@Isoliensinine. Additionally, we conducted comprehensive investigations into the stability of PEG-PLGA@Isoliensinine, in vitro drug release profiles, and in vivo pharmacokinetics. Furthermore, we assessed the antihypertensive efficacy of this nano-system through in vitro experiments on A7R5 cells and in vivo studies using AngII-induced mice. Result: The findings reveal that PEG-PLGA@Isoliensinine significantly improves isoliensinine absorption by A7R5 cells and enhances targeted in vivo distribution. This translates to a more effective reduction of AngII-induced hypertension and vascular smooth muscle proliferation. Conclusion: In this study, we successfully prepared PEG-PLGA@Isoliensinine by nano-precipitation, and we confirmed that PEG-PLGA@Isoliensinine surpasses free isoliensinine in its effectiveness for the treatment of hypertension, as demonstrated through both in vivo and in vitro experiments. Significance: This study lays the foundation for isoliensinine's clinical use in hypertension treatment and vascular lesion protection, offering new insights for enhancing the bioavailability of traditional Chinese medicine components. Importantly, no toxicity was observed, affirming the successful implementation of this innovative drug delivery system in vivo and offers a promising strategy for enhancing the effectiveness of Isoliensinine and propose an innovative avenue for developing novel formulations of traditional Chinese medicine monomers.

Background: Increasing global overweight and obesity rates not only increase the prevalence of myocardial infarction (MI), but also exacerbate ischemic injury and result in worsened prognosis. Currently, there are no drugs that can reverse myocardial damage once MI has occurred, therefore discovering drugs that can potentially limit the extent of ischemic damage to the myocardium is critical. Resveratrol is a polyphenol known for its antioxidant properties, however whether prolonged daily intake of resveratrol during obesity can protect against MI-induced damage remains unexplored. Methods: We established murine models of obesity via high-fat/high-fructose diet, along with daily administrations of resveratrol or vehicle, then performed surgical MI to examine the effects and mechanisms of resveratrol in protecting against myocardial ischemic injury. Results: Daily administration of resveratrol in obese mice robustly protected against myocardial ischemic injury and improved post-MI cardiac function. Resveratrol strongly inhibited oxidative and DNA damage via activating SIRT3/FOXO3a-dependent antioxidant enzymes following MI, which were completely prevented upon administration of 3-TYP, a selective SIRT3 inhibitor. Hence, the cardioprotective effects of prolonged resveratrol intake in protecting obese mice against myocardial ischemic injury was due to reestablishment of intracellular redox homeostasis through activation of SIRT3/FOXO3a signaling pathway. Conclusion: Our findings provide important new evidence that supports the daily intake of resveratrol, especially in those overweight or obese, which can robustly decrease the extent of ischemic damage following MI. Our study therefore provides new mechanistic insight and suggests the therapeutic potential of resveratrol as an invaluable drug in the treatment of ischemic heart diseases.

IntroductionEarly detection of the virus in the environment or in infected pigs is a critical step to stop African swine fever virus (ASFV) transmission. The p22 protein encoded by ASFV KP177R gene has been shown to have no effect on viral replication and virulence and can serve as a molecular marker for distinguishing field virus strains from future candidate KP177R deletion vaccine strains.MethodsThis study established an ASFV detection assay specific for the highly conserved ASFV KP177R gene based on recombinase polymerase amplification (RPA) and the CRISPR/Cas12 reaction system. The KP177R gene served as the initial template for the RPA reaction to generate amplicons, which were recognized by guide RNA to activate the trans-cleavage activity of Cas12a protein, thereby leading to non-specific cleavage of single-stranded DNA as well as corresponding color reaction. The viral detection in this assay could be determined by visualizing the results of fluorescence or lateral flow dipstick (LFD) biotin blotting for color development, and was respectively referred to as fluorescein-labeled RPA-CRISPR/Cas12a and biotin-labeled LFD RPA-CRISPR/Cas12a. The clinical samples were simultaneously subjected to the aforementioned assay, while real-time quantitative PCR (RT-qPCR) was employed as a control for determining the diagnostic concordance rate between both assays.ResultsThe results showed that fluorescein- and biotin-labeled LFD KP177R RPA-CRISPR/Cas12a assays specifically detected ASFV, did not cross-react with other swine pathogens including PCV2, PEDV, PDCoV, and PRV. The detection assay established in this study had a limit of detection (LOD) of 6.8 copies/μL, and both assays were completed in 30 min. The KP177R RPA-CRISPR/Cas12a assay demonstrated a diagnostic coincidence rate of 100% and a kappa value of 1.000 (p < 0.001), with six out of ten clinical samples testing positive for ASFV using both KP177R RPA-CRISPR/Cas12a and RT-qPCR, while four samples tested negative in both assays.DiscussionThe rapid, sensitive and visual detection assay for ASFV developed in this study is suitable for field application in swine farms, particularly for future differentiation of field virus strains from candidate KP177R gene-deleted ASFV vaccines, which may be a valuable screening tool for ASF eradication.

In this study, we conducted a 16-week feeding trial to investigate the effects of a high-cassava starch diet on growth performance, liver function, and metabolism in largemouth bass (Micropterus salmoides). We formulated five diets containing varying levels of cassava starch: 12%, 9%, 6%, 3%, and 0% (termed M12, M9, M6, M3, and M0, respectively). We distributed these diets among largemouth bass with the initial body weight of 83.33 ± 0.55 g via an in-pond “raceway” aquaculture system. Our findings suggest that high level (12%) of cassava starch dietary inclusion adversely affected growth performance metrics such as weight gain rate and specific growth rate, along with feed utilization efficiency indicators, including protein efficiency, protein deposition rate, and the apparent digestibility of dry matter and protein. This negative impact was accompanied by a decrease in intestinal amylase activity. Through further transcriptomic analysis, we identified several key genes associated with carbohydrate metabolism, which underwent changes influencing liver function. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed the involvement of these differentially expressed genes (DEGs) in the tricarboxylic acid cycle (TCA cycle). Comparative metabolomics analysis further indicated that the M9 group showed significant enrichment in pathways related to amino acid metabolism and alterations in the levels of metabolites involved in carbohydrate metabolism. In conclusion, our study demonstrates that incorporating up to 9% cassava starch in the diet can enhance growth performance in largemouth bass by stimulating digestive enzyme activities and promoting glucose utilization.

In this study, the rotating welding process of Chinese fir (Keteleeriafortunei) in Guizhou, China, was systematically analyzed. The effects of rotating welding conditions, including the dowel-to-guide hole diameter ratio, welding time, depth, base surface, angle, and dowel type, on the performance of welded Chinese fir were explored. Moreover, the physical and chemical changes oftheChinese fir interface during welding were revealed by Fourier-Transform Infrared Spectroscopy (FT-IR), X-ray Photoelectron Spectroscopy (XPS), X-ray Diffraction (XRD), and Scanning Electron Microscopy (SEM). The results indicated the following: (1) The rotating welding technology can quickly achieve a strong connection between wood through friction heat without chemical adhesives and compared with traditional wood connection technology such as gluing or mechanical fixing;it has the advantages of simple operation, high production efficiency; and environmental friendliness. (2) Aftertherotating welding, the wood underwent significant pyrolysis, especially the degradation of hemicellulose. The heat generated in the welding process caused good melting and mechanical interlocking between the dowel and the wall of the guide hole, but it was also accompanied by afriction loss of the dowel and the substrate. (3) The welding parameters affected the wood’s connection strength and stability by altering heat production, distribution, transfer, and frictional losses. The impact of the dowel-to-guide hole diameter ratio had a great influence on the connection strength. When the diameter ratio was 1:0.7, the tensile strength was the highest, reaching 2.27 MPa. (4) The analyses of XPS, FTIR, XRD, and SEM proved thatthechemical composition changes at the interface, leading to a more structured crystalline bond and enhanced connection strength due to fiber entanglement and interlocking. This research providesatheoretical and experimental basis forthefurther innovation and development of wood processing technology and provides a new technical path forthegreen manufacturing of wood structure buildings.

MiRNAs are edited or modified in multiple ways during their biogenesis pathways. It was reported that miRNA editing was deregulated in tumors, suggesting the potential value of miRNA editing in cancer classification. Here we extracted three types of miRNA features from 395 LUAD and control samples, including the abundances of original miRNAs, the abundances of edited miRNAs, and the editing levels of miRNA editing sites. Our results show that eight classification algorithms selected generally had better performances on combined features than on the abundances of miRNAs or editing features of miRNAs alone. One feature selection algorithm, i.e., the DFL algorithm, selected only three features, i.e., the frequencies of hsa-miR-135b-5p, hsa-miR-210-3p and hsa-mir-182_48u (an edited miRNA), from 316 training samples. Seven classification algorithms achieved 100% accuracies on these three features for 79 independent testing samples. These results indicate that the additional information of miRNA editing is useful in improving the classification of LUAD samples.

Background: The efficacy and safety of Qingda granule (QDG) in managing blood pressure (BP) among grade 1 hypertensive patients with low-moderate risk remain uncertain. Methods: In the randomized, double-blind, double dummy, non-inferiority and multicenter trial, 552 patients with grade 1 hypertension at low-moderate risk were assigned at a ratio of 1:1 to receive either QDG or valsartan for 4 weeks, followed up by a subsequent 4 weeks. Results: Post-treatment, clinic systolic/diastolic BPs (SBP/DBP) were reduced by a mean change of 9.18/4.04 mm Hg in the QDG group and 9.85/5.05 mm Hg in the valsartan group (SBP P = 0.47, DBP P = 0.16). Similarly, 24-hour, daytime and nighttime BPs were proportional in both groups (P > 0.05) after 4 weeks treatment. After discontinuing medications for 4 weeks, the mean reduction of clinic SBP/DBP were 0.29/0.57 mm Hg in the QDG group compared to −1.59/−0.48 mm Hg in the valsartan group (SBP P = 0.04, DBP P = 0.04). Simultaneously, the 24-hour SBP/DBP were reduced by 0.9/0.31 mm Hg in the QDG group and −1.66/−1.08 mm Hg in the valsartan group (SBP P = 0.006, DBP P = 0.02). And similar results were observed regarding the outcomes of daytime and nighttime BPs. There was no difference in occurrence of adverse events between two groups (P > 0.05). Conclusion: QDG proves to be efficacious for grade 1 hypertension at a low-to-medium risk, even after discontinuation of the medication for 4 weeks. These findings provide a promising option for managing grade 1 hypertension and suggest the potential for maintaining stable BP through intermittent administration of QDG. Trial registration: ChiCTR2000033890.

A single-row plasma tube array (PTA) experimental system is established to improve the backscattering attenuation of a metal plate covered by a plasma tube array. The backscattering test system is utilized in a microwave anechoic chamber to examine the effects of gas composition, pressure, tube diameter, and discharge power on the backscattering attenuation of a metal plate using a plasma tube array. The electron density is obtained via microwave diagnosis. The backscattering attenuation mechanism in different frequency bands is revealed via numerical simulation. The results show that the reasonable selection of PTA parameters achieves strong attenuation in different frequency bands. The strong attenuation frequency bands of Ar–Hg PTA are in low frequency (1.5–3.5 GHz) and high frequency (13–17 GHz), while that of Ne–Hg discharge is in medium frequency (6.4–11.7 GHz). When the pressure is 0.5 and 1 Torr, the PTA shows a low, medium, and high multi-band distribution for the backscattering strong attenuation region. The backscattering strong attenuation region shows a low and high dual-band distribution, while the pressure is 2–4 Torr. As the tube diameter increases, the strong attenuation region maintains the dual-band, but it changes from low and high frequency bands to medium frequency (6-12 GHz), where the backscattering attenuation mechanism is collisional absorption when the frequency of plasma electron oscillation is close to that of electro-magnetic waves. The backscattering attenuation mechanism in the low frequency band involves the periodic structure of PTA generating local surface plasmon to absorb electromagnetic waves.

Out-of-face gradient sandwich structures have been widely studied for their excellent impact resistance. One uniform and two out-of-plane gradient cores are proposed based on the bionic structure of Royal Water Lily, and the midspan deflection of the back panel and the energy absorption of the out-of-plane gradient sandwich structures under various blast loads are studied. Two frequently adopted methods of explosive loading are applied to the sandwich panels, and the responses are contrasted for the loads applied as a time-dependent pressure history versus imposition of the initial velocity. The effect of the fluid–structure interaction is considered in the blast impulsion, and the dynamic responses of the sandwich structures with different out-of-plane density arrangements are analyzed under two loading approaches. Results show that the energy absorption of the core layer under the prescribed velocity approach is approximately 3–5 times that of the applied pressure approach, while the back panel deflections of different out-of-plane gradient sandwiches are similar. There are significant differences in the deformation mechanisms of structures under these two types of impact loads. Under the same type of impact load, the core compression process of the out-of-plane positive gradient sandwich panel is decoupled from the whole tensile bending deformation process of the structure, whereas the core compression process of the out-of-plane negative gradient sandwich panel is strongly coupled with the whole tensile bending deformation process of the structure. The related research will lay the foundation for an in-depth understanding of the theoretical study of the impact of out-of-face gradient sandwich structures.

Terahertz (THz) sensors have attracted great attention in the biological field due to their nondestructive and contact-free biochemical samples. Recently, the concept of a quasi-bound state in the continuum (QBIC) has gained significant attention in designing biosensors with ultrahigh sensitivity. QBIC-based metasurfaces (MSs) achieve excellent performance in various applications, including sensing, optical switching, and laser, providing a reliable platform for biomaterial sensors with terahertz radiation. In this study, a structure-engineered THz MS consisting of a “double C” array has been designed, in which an asymmetry parameter α is introduced into the structure by changing the length of one subunit; the Q-factor of the QBIC device can be optimized by engineering the asymmetry parameter α. Theoretical calculation with coupling equations can well reproduce the THz transmission spectra of the designed THz QBIC MS obtained from the numerical simulation. Experimentally, we adopt an MS with α = 0.44 for testing arginine molecules. The experimental results show that different concentrations of arginine molecules lead to significant transmission changes near QBIC resonant frequencies, and the amplitude change is shown to be 16 times higher than that of the classical dipole resonance. The direct limit of detection for arginine molecules on the QBIC MS reaches 0.36 ng/mL. This work provides a new way to realize rapid, accurate, and nondestructive sensing of trace molecules and has potential application in biomaterial detection.

The task of aspect-based sentiment analysis (ASBA) is to identify all the sentiment analyses expressed by specific aspect words in the text. How to identify specific objects (i.e., aspect words), describe the modifiers of the specific objects (i.e., opinion words), and judge the sentiment analysis expressed by opinion words (sentimental classification) in one step has become a focus of research in ASBA. ASTE (Aspect Sentiment Triplet Extraction) based on DREN (Deep Relationship Enhancement Networks) has been proposed in this paper. It aims to extract the aspect words and opinion words in the review text in one-step. They can judge the sentiment analysis expressed by the opinion words. Therefore, the study defines ten kinds of word relations; then, the study uses the parts of the speech feature, syntactic feature, relative position feature and tree distance relative feature to enhance the word representation relationship, which enriches the table of information in the relational matrix. Secondly, based on the word representation of BERT and GCN, the structural information of the texts are extracted; then, further extraction of higher-level word semantic information and word relationship information through SWDA (Sliding Window Dilated Attention) occurs, as SWDA can capture the multi-granularity relationship in words. Finally, the experimental results show that the proposed method is effective.

Soft pneumatic grippers can grasp soft or irregularly shaped objects, indicating potential applications in industry, agriculture, and healthcare. However, soft grippers rarely carry heavy and dense objects due to the intrinsic low modulus of soft materials in nature. This paper designed a soft robotic gripper with rigid supports to enhance lifting force by 150 ± 20% in comparison with that of the same gripper without supports, which successfully lifted a metallic wrench (672 g). The soft gripper also achieves excellent adaptivity for irregularly shaped objects. The design, fabrication, and performance of soft grippers with rigid supports are discussed in this paper.

Aeolian sediments accumulated along the desert-loess transition zone of the Tengger Desert include heterogeneous textures and complex component structures in their grain-size distributions (GSD). However, the sources of these aeolian sediments have not been resolved due to the lack of large reference GSD sample datasets from adjacent regions that contain various types of sediments; such datasets could be used for fingerprinting based on grain-size properties. This lack of knowledge hinders our understanding of the mechanism of aeolian dust releases in these regions and the effects of forcing of atmospheric circulations on the transportation and accumulation of sediments in this region. In this study, we employed a multi-scale grain-size analysis method, i.e., a combination of the single-sample unmixing (SSU) and the parametric end-member modelling (PEMM) techniques, to resolve the component structures of sediments that had accumulated along the desert-loess transition zone of the Tengger Desert. We have also analyzed the component structures of GSDs of various types of sediments, including mobile and fixed sand dunes, lake sediments, and loess sediments from surrounding regions. Our results demonstrate that the patterns observed in coarser fractions of sediments (i.e., sediments with a mode grain size of >100 μm) from the transition zone match well with the patterns of component structures of several types of sediments from the interior of the Tengger Desert, and the patterns seen in the finer fractions (i.e., fine, medium, and coarse silts with a modal size of

Pellet ore is an important raw material for blast furnace ironmaking, and its reduction and expansion performance directly affects the smooth operation and smelting indicators of the blast furnace. This paper quantitatively studied the effects of magnesium minerals such as dolomite and serpentinite on the pellet-forming performance, the microstructure after roasting, compressive strength, and the reduction expansion performance of Baiyun Ebo iron concentrate. The optimal ratio of dolomite and serpentinite to add was determined when preparing pellets using Baiyun Ebo iron concentrate powder. The results showed that the drop strength and compressive strength of the green pellet after adding serpentine were relatively higher than those after adding dolomite, indicating that controlling the MgO content in the green pellet at 2.5% using serpentine was beneficial for improving the drop strength and compressive strength. Under the condition of adding dolomite, when the MgO content was 2.5%, the compressive strength of the roasted pellet was the highest, which was 2192.6 N, and the volume expansion rate was 12.32%. Under the condition of adding serpentine, when the MgO content was 2.5%, the compressive strength of the roasted pellet was 2622.2 N, and the volume expansion rate was 9.71%. Compared with dolomite as a magnesium additive, when the reduction expansion rate of Baiyun Ebo iron concentrate was controlled within 20%, serpentine only needed to have a MgO content of about 1.5% in the pellets, while dolomite needed to have a MgO content of about 2.5%. Therefore, under the condition that the MgO contents of dolomite and serpentine were equivalent, the amount of serpentine used was lower.

The timely identification of rail internal defects and the application of corresponding preventive measures would greatly reduce catastrophic failures, such as rail breakage. Ultrasonic rail defect detection is the current mainstream rail defect detection method thanks to its advantages of strong penetration, high accuracy, and ease to deploy. The 2D B-scan image output by ultrasonic detectors contains rich features of defects; however, rail engineers manually identify and localize the defect image, which can be time-consuming, and the image may be subject to missing identification or mistakes. This paper adopted state-of-the-art deep learning algorithms for novel B-scan images for the automatic identification and localization of rail internal tracks. First, through image pre-processing of classification, denoising, and augmentation, four categories of defect image datasets were established, namely crescent-shaped fatigue cracks, fishbolt hole cracks, rail web cracks, and rail base transverse cracks; then, four representatives of deep learning object detection networks, YOLOv8, YOLOv5, DETR, and Faster R-CNN, were trained with the defects dataset and further applied to the testing dataset for defect identification; finally, the performances of the three detection networks were compared and evaluated at the data level, the network structure level, and the interference adaptability level, respectively. The results show that the YOLOv8 network can effectively classify and localize four categories of internal rail defects in B-scan images with a 93.3% mean average precision at three images per second, and the detection time is 58.9%, 376.8%, and 123.2% faster than YOLO v5, DETR, and Faster R-CNN, respectively. The proposed approach could ensure the real-time, accurate, and efficient detection and analysis of internal defects to a rail.

Project-based learning (PjBL) has been increasingly promoted and extended to online environments to enhance the quality of higher education. However, PjBL involves complex processes requiring higher-order thinking skills, which may pose challenges to many students especially in online settings with little prompt support from teachers. The problem may compromise the learning of low-achieving students, who often have inadequate higher-order thinking skills. Visible thinking approaches have the potential to make higher-order thinking processes accessible to students. This study was conducted with 72 university students who engaged in visible thinking supported online PjBL of computer programming. A one-group pretest-posttest design was adopted to compare the learning outcomes among high-, medium- and low-achieving students. The results showed that compared to high and medium achievers, low-achieving students made the most progress in product quality and thinking skills (in particular process design skills). They performed almost as well as medium and high achievers in product quality and process design skills at the end of the study. They also gained more knowledge from the project than high achievers did. Moreover, compared to medium achievers, low achievers perceived the approach as more valuable, made more effort on the study, and felt more competent in completing the project. The findings reveal the promising effects of visualizing higher-order thinking processes in narrowing the achievement gap between high and low achievers, offering all students an equal chance to engage in effective learning with projects.