The increasing demand for natural bioactive compounds necessitates the exploration of underutilized plant species for their therapeutic potential. Plantago coronopussubsp. commutata(Guss.) Pilg. (Synonym: Plantago weldeniiRchb.) is a promising candidate for its phytochemical and pharmacological properties but remains underexplored. This study aimed to investigate the chemical composition, antioxidant activities, and enzyme inhibitory properties of water, methanol, and ethyl acetate extracts of P. coronopussubsp. commutata. Phytochemicals responsible for these bioactivities were identified using quantitative and qualitative methods, including LC–MS/MS analysis. Methanol extraction yielded the highest total phenolic (TPC: 36.30 mg GAEs/g) and flavonoid contents (TFC: 24.75 mg REs/g), alongside a rich phytochemical profile, particularly verbascoside (46,037 μg/g) and luteolin 7-glucoside (84.1 μg/g). Methanol extract exhibited the most effective antioxidant activity, with IC50values of 1.12 mg/mL (DPPH), 1.62 mg/mL (ABTS), and an EC50of 0.93 mg/mL (CUPRAC). Ethyl acetate extract demonstrated the highest total antioxidant capacity (EC50: 1.47 mg/mL), whereas the water extract excelled in metal chelation activity (IC50: 1.30 mg/mL). Correlation analysis indicated strong positive associations between TPC, TFC, and antioxidant performance. Enzyme inhibition assays revealed significant α-glucosidase inhibition in the ethyl acetate extract (IC50: 1.07 mg/mL) and notable tyrosinase inhibition in the methanol extract (IC50: 1.39 mg/mL). Verbascoside and phenolic acids were identified as major contributors to these effects. This study provides the first comprehensive evaluation of P. coronopussubsp. commutata, highlighting its phytochemical richness and strong antioxidant and enzyme inhibitory potential. These findings suggest the plant's promising pharmaceutical applications, encouraging future studies to explore its in vivo effects and isolate bioactive compounds for targeted therapeutic use.