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The giant freshwater prawn (Macrobrachium rosenbergii) has many advantages in aquaculture, such as fast growth rate, short breeding cycle and good nutrition, which makes it a freshwater shrimp with high economic value. Herein, high-quality chromosome-level genome of both female and male prawns were obtained by combining Illumina paired-end sequencing, PacBio single molecule sequencing technique and High-through chromosome conformation capture (Hi-C) technologies. In ZZ male prawn, a final contig assembly of 3118.58 Mb with a N50 length of 956,237 bp was obtained. In WW female prawn, a final contig assembly of 3333.31 Mb with a N50 length of 1,143,555 bp was obtained. The assembled genome sequences from prawns were anchored to 59 chromosomes. Moreover, the sex chromosomes including W chromosome and Z chromosome were generated in prawn with the length of 36.23 Mb and 27.33 Mb, respectively. The sequence similarity of Z chromosome and W chromosome reached to 74.90%. The high-quality genome resource will be useful for further molecular breeding and functional genomic research of giant freshwater prawns. [ABSTRACT FROM AUTHOR]

In this study, nano- SiO2 was synthesized using the sol-gel method to modify the properties of fly ash-slag geopolymers (FASGs). FASGs with six contents of sol-type nano- SiO2 (0, 0.1%, 0.2%, 0.3%, 0.4%, and 0.5%) and two alkali dosages (5% and 7% of Na2O) were prepared. The influences of sol-type nano- SiO2 and alkali content on the compressive strength, flexural strength, drying shrinkage, and microstructure of FASG were studied. The results demonstrated that the early-age compressive and flexure strength of FASG can be enhanced by sol-type nano- SiO2 due to the improved microstructure and the reaction products. The improvement effect of sol-type nano- SiO2 on mechanical properties was more significant for FASGs with lower alkali dosages. The optimized content of sol-type nano- SiO2 in FASG was 0.2% for the mechanical properties. The addition of the sol-type nano- SiO2 can mitigate the drying shrinkage of the FASG when the content is lower than 0.4%. The sol-type nano- SiO2 can promote the formation of reaction products in FASG and result in a denser microstructure of FASG. [ABSTRACT FROM AUTHOR]

Yellowfin tuna (Thunnus albacares) is a valuable pelagic migratory fish with potential for aquaculture. Despite this, there is limited understanding of the biological and physiological characteristics of this species, particularly regarding sex differences in growth performance. The liver, a crucial organ for digestion and metabolism, plays a significant role in regulating fish growth. This study aimed to compare liver enzyme activities and transcriptome profiles between female and male yellowfin tuna to uncover the molecular mechanisms underlying difference between the sexes. The results revealed that female yellowfin tuna exhibited higher amylase and lipid metabolism enzyme activities, while male yellowfin tuna showed higher glucose-6-phosphate dehydrogenase and antioxidant enzyme activities. Additionally, through Illumina sequencing technology, the study generated 37.74 Gb of clean data and identified 36,482 unique genes (UniGenes) in the liver transcriptome. A total of 2542 differentially expressed genes were found, with enriched Gene Ontology terms and pathways related to metabolic processes, particularly lipid metabolism and transport. These findings suggest that female yellowfin tuna have superior digestive enzyme activities and lipid metabolism, while male yellowfin tuna excel in sugar metabolism, ATP production, and antioxidant defense. This study provides valuable insights into sex differences in yellowfin tuna and could aid in advancing full-cycle aquaculture practices for this species. [ABSTRACT FROM AUTHOR]