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This experiment was conducted in two separate parts of the farm and the laboratory to evaluate effect of sowing date and seed hydropriming on, seed cotton yield, lint and seed oil of two cotton cultivars using a factorial experiment based on randomized complete block design in three replications at the Research Farm of Shahed University's Faculty of Agriculture in the spring of 2018. In the laboratory section, the effects of hydropriming and two cotton varieties on the maternal seeds obtained from the first study were investigated. In the field experiment the first factor was sowing date with three levels: April 14, April 30 and May 15, the second factor was hydropriming in two levels of control (without priming) and hydro-priming application and the third factor was cultivar with two levels of May344 and Sajedi cultivars. In a laboratory study that was conducted as a two-factor factorial in the form of a completely randomized design, in three replications, the factors included hydropriming in two control levels (without priming) and hydropriming and cotton cultivars including Sajedi and May344. The Results of the field this study showed that hydropriming had no effects on weight of single boll. The weight of bolls on the sowing date of May 15 compared to April 14 was higher in the condition without priming in both cultivars. But hydropriming increased seed yield. The highest seed yield was obtained with average 11.07 ton/ha in the primed seeds of May344 variety on April 14, which were more 26% increase compared to the nonpriming treatment and compared to April 30 and May 15, respectively 9.7 and 28.8 percent. The comparison results of the average sowing date and priming showed that the planting date of 30 April had the highest percentage of seed oil. Highest percentage of oil with an average of 23.4% was observed on this sowing date and in without priming treatment. The results of laboratory study showed that priming improved the germination of seeds in both cultivars, but in Sajedi cultivar (with an average germination percentage of 97.5%) compared to MAY 344 cultivar, the germination percentage was 36.1% higher. Other characteristics of germination such as fresh and dry weight of seedling, length of root and shoot, length and weight index of seeds improved under the effect of priming in both cultivars. [ABSTRACT FROM AUTHOR]