5 results on '"Liu, Ruozhuo"'
Search Results
2. Alleviation of migraine related pain and anxiety by inhibiting calcium-stimulating AC1-dependent CGRP in the insula of adult rats.
- Author
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Li, Yang, Li, Chenhao, Chen, Qi-Yu, Hao, Shun, Mao, Jingrui, Zhang, Wenwen, Han, Xun, Dong, Zhao, Liu, Ruozhuo, Tang, Wenjing, Zhuo, Min, Yu, Shengyuan, and Liu, Yinglu
- Subjects
MIGRAINE prevention ,ANXIETY prevention ,ENZYME inhibitors ,BIOLOGICAL models ,CHRONIC pain ,INTRAPERITONEAL injections ,INSULAR cortex ,RESEARCH funding ,TREATMENT effectiveness ,DESCRIPTIVE statistics ,FLUORESCENT antibody technique ,NEURAL transmission ,CELLULAR signal transduction ,RATS ,CALCIUM ,NEUROPEPTIDES ,ANIMAL experimentation ,WESTERN immunoblotting ,PAIN management ,COMPARATIVE studies ,CELL receptors ,EVALUATION - Abstract
Background: Recent animal and clinical findings consistently highlight the critical role of calcitonin gene-related peptide (CGRP) in chronic migraine (CM) and related emotional responses. CGRP antibodies and receptor antagonists have been approved for CM treatment. However, the underlying CGRP-related signaling pathways in the pain-related cortex remain poorly understood. Methods: The SD rats were used to establish the CM model by dural infusions of inflammatory soup. Periorbital mechanical thresholds were assessed using von-Frey filaments, and anxiety-like behaviors were observed via open field and elevated plus maze tests. Expression of c-Fos, CGRP and NMDA GluN2B receptors was detected using immunofluorescence and western blotting analyses. The excitatory synaptic transmission was detected by whole-cell patch-clamp recording. A human-used adenylate cyclase 1 (AC1) inhibitor, hNB001, was applied via insula stereotaxic and intraperitoneal injections in CM rats. Results: The insular cortex (IC) was activated in the migraine model rats. Glutamate-mediated excitatory transmission and NMDA GluN2B receptors in the IC were potentiated. CGRP levels in the IC significantly increased during nociceptive and anxiety-like activities. Locally applied hNB001 in the IC or intraperitoneally alleviated periorbital mechanical thresholds and anxiety behaviors in migraine rats. Furthermore, CGRP expression in the IC decreased after the hNB001 application. Conclusions: Our study indicated that AC1-dependent IC plasticity contributes to migraine and AC1 may be a promising target for treating migraine in the future. [ABSTRACT FROM AUTHOR]
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- 2024
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3. Migraine-like headache in subjects with isolated Lambl’s excrescences: a case series and literature review
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Xie, Wei, primary, Wang, Xiaoguang, additional, Li, Ruibing, additional, Jia, Zhihua, additional, Miao, Shuai, additional, Liu, Yang, additional, Yang, Chunxiao, additional, Li, Chenhao, additional, Zhao, He, additional, Yu, Shengyuan, additional, and Liu, Ruozhuo, additional
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- 2024
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4. Brain-wide mapping of c-Fos expression in nitroglycerin-induced models of migraine.
- Author
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Xiao S, Lu G, Liu J, Su W, Li C, Liu Y, Meng F, Zhao J, Gao N, Chang Y, Guo X, Yu S, and Liu R
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- Animals, Mice, Male, Proto-Oncogene Proteins c-fos metabolism, Mice, Inbred C57BL, Brain Mapping, Vasodilator Agents pharmacology, Vasodilator Agents administration & dosage, Pain Threshold drug effects, Nitroglycerin toxicity, Nitroglycerin pharmacology, Nitroglycerin administration & dosage, Migraine Disorders chemically induced, Migraine Disorders diagnostic imaging, Migraine Disorders metabolism, Migraine Disorders drug therapy, Disease Models, Animal, Brain diagnostic imaging, Brain drug effects, Brain metabolism
- Abstract
Background: Migraine is a neurological disorder characterized by complex, widespread, and sudden attacks with an unclear pathogenesis, particularly in chronic migraine (CM). Specific brain regions, including the insula, amygdala, thalamus, and cingulate, medial prefrontal, and anterior cingulate cortex, are commonly activated by pain stimuli in patients with CM and animal models. This study employs fluorescence microscopy optical sectioning tomography (fMOST) technology and AAV-PHP.eB whole-brain expression to map activation patterns of brain regions in CM mice, thus enhancing the understanding of CM pathogenesis and suggesting potential treatment targets., Methods: By repeatedly administering nitroglycerin (NTG) to induce migraine-like pain in mice, a chronic migraine model (CMM) was established. Olcegepant (OLC) was then used as treatment and its effects on mechanical pain hypersensitivity and brain region activation were observed. All mice underwent mechanical withdrawal threshold, light-aversive, and elevated plus maze tests. Viral injections were administered to the mice one month prior to modelling, and brain samples were collected 2 h after the final NTG/vehicle control injection for whole-brain imaging using fMOST., Results: In the NTG-induced CMM, mechanical pain threshold decreased, photophobia, and anxiety-like behavior were observed, and OLC was found to improve these manifestations. fMOST whole-brain imaging results suggest that the isocortex-cerebral cortex plate region, including somatomotor areas (MO), somatosensory areas (SS), and main olfactory bulb (MOB), appears to be the most sensitive area of activation in CM (P < 0.05). Other brain regions such as the inferior colliculus (IC) and intermediate reticular nucleus (IRN) were also exhibited significant activation (P < 0.05). The improvement in migraine-like symptoms observed with OLC treatment may be related to its effects on these brain regions, particularly SS, MO, ansiform lobule (AN), IC, spinal nucleus of the trigeminal, caudal part (Sp5c), IRN, and parvicellular reticular nucleus (PARN) (P < 0.05)., Conclusions: fMOST whole-brain imaging reveals c-Fos + cells in numerous brain regions. OLC improves migraine-like symptoms by modulating brain activity in some brain regions. This study demonstrates the activation of the specific brain areas in NTG-induced CMM and suggests some regions as a potential treatment mechanism according to OLC., (© 2024. The Author(s).)
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- 2024
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5. Profile of Chinese Cluster Headache Register Individual Study (CHRIS): Clinical characteristics, diagnosis and treatment status data of 816 patients in China.
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Zhang S, Xu S, Chen C, Xue Z, Yao Y, Zhao H, Zhao H, Ji Y, Wang D, Hu D, Liu K, Chen J, Chen S, Gao X, Gui W, Fan Z, Wan D, Yuan X, Qu W, Xiao Z, Dong M, Wang H, Ju C, Xu H, Zhang L, Wang X, Zhang M, Han X, Ran Y, Jia Z, Su H, Li Y, Liu H, Zhao W, Gong Z, Lin X, Liu Y, Sun Y, Xie S, Zhai D, Liu R, Wang S, Dong Z, and Yu S
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- Female, Humans, Male, China epidemiology, Longitudinal Studies, Prospective Studies, Adult, Cluster Headache diagnosis, Cluster Headache epidemiology, Cluster Headache therapy
- Abstract
Background: The clinical profile of cluster headache may differ among different regions of the world, warranting interest in the data obtained from the initial Chinese Cluster Headache Register Individual Study (CHRIS) for better understanding., Methods: We conducted a multicenter, prospective, longitudinal cohort study on cluster headache across all 31 provinces of China, aiming to gather clinical characteristics, treatment approaches, imaging, electrophysiological and biological samples., Results: In total 816 patients were enrolled with a male-to-female ratio of 4.33:1. The mean age at consultation was 34.98 ± 9.91 years, and 24.89 ± 9.77 years at onset. Only 2.33% were diagnosed with chronic cluster headache, and 6.99% had a family history of the condition. The most common bout was one to two times per year (45.96%), lasting two weeks to one month (44.00%), and occurring frequently in spring (76.23%) and winter (73.04%). Of these, 68.50% experienced one to two attacks per day, with the majority lasting one to two hours (45.59%). The most common time for attacks was between 9 am and 12 pm (75.86%), followed by 1 am and 3 am (43.48%). Lacrimation (78.80%) was the most predominant autonomic symptom reported. Furthermore, 39.22% of patients experienced a delay of 10 years or more in receiving a correct diagnosis. Only 35.67% and 24.26% of patients received common acute and preventive treatments, respectively., Conclusion: Due to differences in ethnicity, genetics and lifestyle conditions, CHRIS has provided valuable baseline data from China. By establishing a dynamic cohort with comprehensive multidimensional data, it aims to advance the management system for cluster headache in China., Competing Interests: Declaration of conflicting interestsThe authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
- Published
- 2024
- Full Text
- View/download PDF
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