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[EN] This is the experimental dataset used in the paper “Barrier Graphene Oxide on a CoCr Alloy via Silane/GO Covalent Bonding and Its Electrochemical Behavior in a Simulated Synovial Fluid Electrolyte". The article includes the experimental procedure carried out for the deposition of graphene oxide on CoCr surfaces: CoCr was hydroxylated through alkalization in NaOH 5 M for 2 h, (sample named CoCr-OH). Subsequently, silanization was carried out using APTES. APTES was pre-mixed at 2% vol in isopropanol-water (200:1 v/v) and stirred for 1 h. After this, CoCr-OH samples were immersed in the APTES solution at room temperature for 1 min. The silane-coated samples were kept under curing conditions at 45 °C (CoCr-OH-Si45 sample) and 75 °C (CoCr-OH-Si75 sample) for 24 h and 30 min, respectively. Finally, CoCr-OH-Si45 and CoCr-OH-Si75 surfaces were immersed in 4 g/L GO aqueous suspension at 60 °C for 12 h and 24 h to obtain CoCr-OH-Si45-GO12h, CoCr-OH-Si75-GO12h and CoCr-OH-Si45-GO24h, and CoCr-OH-Si75-GO24h. This data set includes the results of the electrochemical impedance data obtained for each CoCr surface: CoCr (CoCr, CoCr-OH, CoCr-OHSi45, CoCr-OH-Si75 CoCr-OH-Si45-GO12h, CoCr-OH-Si75-GO12h y CoCr-OH-Si45-GO24h, y CoCr-OH-Si75-GO24h, immersed in 3 g/L Hyaluronic acid solution for 7 days., [ES] Este es el conjunto de datos experimentales utilizado en el artículo "Barrera de Óxido de Grafeno en una Aleación CoCr mediante enlace covalente de Silano/GO y su comportamiento electroquímico en un electrolito de fluido sinovial simulado". El artículo incluye el procedimiento experimental llevado a cabo para la deposición de óxido de grafeno sobre superficies de CoCr: CoCr fue hidroxilado mediante alcalinización en NaOH 5 M durante 2 horas (muestra denominada CoCr-OH). Posteriormente, se llevó a cabo la silanización utilizando APTES. APTES fue premezclado al 2% en volumen en isopropanol-agua (200:1 v/v) y agitado durante 1 hora. Después, las muestras de CoCr-OH fueron sumergidas en la solución de APTES a temperatura ambiente durante 1 minuto. Las muestras recubiertas con silano se mantuvieron bajo condiciones de curado a 45 °C (muestra CoCr-OH-Si45) y 75 °C (muestra CoCr-OH-Si75) durante 24 horas y 30 minutos, respectivamente. Finalmente, las superficies CoCr-OH-Si45 y CoCr-OH-Si75 fueron sumergidas en suspensión acuosa de 4 g/L GO a 60 °C durante 12 y 24 horas obteniendo las superficies CoCr-OH-Si45-GO12h, CoCr-OH-Si75-GO12h y CoCr-OH-Si45-GO24h, y CoCr-OH-Si75-GO24h. Este conjunto de datos incluye los resultados de los datos de impedancia electroquímica obtenidos para cada superficie de CoCr (CoCr, CoCr-OH, CoCr-OHSi45, CoCr-OH-Si75 CoCr-OH-Si45-GO12h, CoCr-OH-Si75-GO12h y CoCr-OH-Si45-GO24h, y CoCr-OH-Si75-GO24h sumergidas en una solución de 3 g/L ácido hialurónico durante 7 días.

[EN] This is the experimental dataset used in the paper “Macrophage Proteomic Analysis of Covalent Immobilization of Hyaluronic Acid and Graphene Oxide on CoCr Alloy in a Tribocorrosive Environment". The article includes the experimental procedure carried out for the deposition of graphene oxide and posterior immobilization of hyaluronic acid on CoCr surfaces. CoCr was hydroxylated through alkalization in NaOH 5 M for 2 h. Subsequently, silanization was carried out using APTES. APTES was pre-mixed at 2% vol in isopropanol-water (200:1 v/v) and stirred for 1 h. After this, CoCr-OH samples were immersed in the APTES solution at room temperature for 1 min. The silane-coated samples were kept under curing conditions at 45 °C for 24 h. Then, silanized CoCr surfaces were immersed in 4 g/L GO aqueous suspension at 60 °C for 24 h. Carboxylic groups of graphene oxide in GO/CoCr samples were activated by immersing in distilled water (50 mL) with N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC, 250 mg) and N-hydroxysuccinimide (NHS, 750 mg) during 24 h under mild orbital saking. After that, the GO/CoCr surfaces with activated carboxyl groups were immersed in distilled water (100 mL) with dissolved adipic dihydrazide (ADH, 1.04 g) and let to react for another 24 h under mild orbital shaking. Pre-activated HA-(EDC/NHS) solution was obtained by dissolving HA (100 mg), EDC (253 mg) and NHS (754 mg) in 100 mL phosphate buffer saline (PBS) and stirred for 24h to activate the carboxylic acids of HA. After that, ADH/GO/CoCr surfaces were immersed in pre-activated HA-(EDC/NHS) solution and let to react for 24h under mild orbital motion (samples named HA/GO/CoCr). This data set includes the results of the final surface by Raman Spectroscopy and Electrochemical Impedance Spectroscopy. Raman was obtained from CoCr/GO/HA surfaces in comparison with GO/CoCr surfaces and HA powder. Electrochemical impedance data of CoCr/GO/HA were obtained under immersion in 3 g/L Hyaluronic, [ES] Este es el conjunto de datos experimentales utilizado en el artículo " Macrophage Proteomic Analysis of Covalent Immobilization of Hyaluronic Acid and Graphene Oxide on CoCr Alloy in a Tribocorrosive Environment". El artículo incluye el procedimiento experimental realizado para la deposición de óxido de grafeno y la posterior inmovilización de ácido hialurónico sobre superficies de CoCr. Las superficies de CoCr fueron hidroxiladas mediante alcalinización en NaOH 5 M durante 2 h. Posteriormente, se realizó la silanización utilizando APTES premezclado al 2% en volumen en isopropanol-agua (200:1 v/v) agitada durante 1 h. Las muestras de CoCr hidroxiladas se sumergieron en la solución de APTES a temperatura ambiente durante 1 minuto. Posteriormente, se mantuvieron en condiciones de curado a 45 °C durante 24 h. Transcurrido este tiempo, las superficies de CoCr silanizadas se sumergieron en una suspensión acuosa de 4g/L GO a 60 °C durante 24 h. Los grupos carboxílicos del óxido de grafeno en las muestras GO/CoCr se activaron mediante inmersión en 50 mL agua destilada que contenía hidrocloruro de N-(3-dimetilaminopropil)-N-etilcarbodiimida (EDC, 250 mg) y N-hidroxisuccinimida (NHS, 750 mg), durante 24 h en agitación orbital suave. Las superficies de GO/CoCr con grupos carboxílicos activados se sumergieron en 100 mL de agua destilada con dihidracida adipídica disuelta (ADH, 1.04 g) y se dejaron reaccionar durante otras 24 h en agitación orbital suave. La solución de HA preactivada (EDC/NHS) se obtuvo disolviendo HA (100 mg), EDC (253 mg) y NHS (754 mg) en 100 mL de solución salina de fosfato (PBS) y agitando durante 24 h para activar los ácidos carboxílicos de la HA. Después, las superficies de ADH/GO/CoCr se sumergieron en la solución de HA preactivada (EDC/NHS) y se dejaron reaccionar durante 24 h en movimiento orbital suave (muestras denominadas HA/GO/CoCr). Este conjunto de datos incluye los resultados de la caracterización superficial final mediante espectroscopía

Producción Científica, Sepsis provokes an induction of inducible nitric oxide synthase (iNOS) and melatonin down-regulates its expression and activity. Looking for an inducible mtNOS isoform, we induced sepsis by cecal ligation and puncture in both normal and iNOS knockout mice and studied the changes in mtNOS activity. We also studied the effects of mtNOS induction in mitochondrial function, and the role of melatonin against induced mtNOS and mitochondrial dysfunction. The activity of mtNOS and nitrite levels significantly increased after sepsis in iNOS+/+ mice. These animals showed a significant inhibition of the respiratory chain activity and an increase in mitochondrial oxidative stress, reflected in the disulfide/glutathione ratio, glutathione redox cycling enzymes activity and lipid peroxidation levels. Interestingly, mtNOS activity remained unchanged in iNOS-/- septic mice, and mitochondria of these animals were unaffected by sepsis. Melatonin administration to iNOS+/+ mice counteracted mtNOS induction and respiratory chain failure, restoring the redox status. The results support the existence of an inducible mtNOS that is likely coded by the same gene as iNOS. The results also suggest that sepsis-induced mtNOS is responsible for the increase of mitochondrial impairment due to oxidative stress in sepsis, perhaps due to the high production of NO. Melatonin treatment prevents mitochondrial failure at the same extend as the lack of iNOS gene., This study was partially supported by grants from the Instituto de Salud Carlos III (grants FIS01/1076, PI03/0817 and G03/137), and Consejer´ıa de Educacion, ´ Junta de Andaluc´ıa (CTS-101). LCL, VT and JL are fellows from the Instituto de Salud Carlos III (Spain).

Within the framework of the Sustainable Development Goals of the Agenda 2030, the circular economy is being promoted as a means of ensuring a sustainable use of resources and a reduction in the amount of waste produced. The aim is to reduce the demand for often scarce raw materials through the continuous reuse, recycling and regeneration of materials and products. This paper explores the use of carbon fibre from wind turbine blades as an additive in the production of new efficient bricks. Clay mixes with 0, 5 and 10 wt% additive were fired at three temperatures (800, 950 and 1100 °C) and the fired bricks were analysed from mineralogical and physical points of view to determine their suitability for use in the construction industry. The results show that carbon fibre improves the durability of the bricks, which became 16 % more porous as the firing temperature increased. However, the compressive strength of the bricks with 10 wt% carbon fibre was about 50 % lower than that of the control bricks made without additive. It is interesting to note that the distribution of the carbon fibres within the brick varies considerably and that they are shorter and wider in the core of the samples. These results could offer an alternative line for new product development in the brick industry. The bricks tested here are an example of a circular economy in which waste from one industrial process (wind turbine blades) is reused as an input in another (brick manufacture). The environmental benefits achieved are twofold: reduced demand for clay and recycling of decommissioned turbine blades, which are currently amassed in wind turbine graveyards.

In this work, a sequential covalent co-immobilization of graphene oxide (GO) and hyaluronic acid (HA) is performed to obtain a biocompatible wear-resistant nanocoating on the surface of the biomedical grade Cobalt-Chrome (CoCr) alloy. Nanocoated CoCr surfaces were characterized by Raman spectroscopy and electrochemical impedance spectroscopy (EIS) in 3 g/L HA electrolyte. Tribocorrosion tests of the nanocoated CoCr surfaces were carried out in a pin on flat tribometer. The biological response of covalently HA/GO biofunctionalized CoCr surfaces with and without wear-corrosion processes was studied through the analysis of the proteome of macrophages. Raman spectra revealed characteristic bands of GO and HA on the functionalized CoCr surfaces. The electrochemical response by EIS showed a stable and protective behavior over 23 days in the simulated biological environment. HA/GO covalently immobilized on CoCr alloy is able to protect the surface and reduce the wear volume released under tribocorrosion tests. Unsupervised classification analysis of the macrophage proteome via Hierarchical clustering and Principal Component Analysis (PCA) revealed that the covalent functionalization on CoCr enhances the macrophage biocompatibility in vitro. On the other hand, disruption of the HA/GO nanocoating by tribocorrosion processes induced a macrophage proteome which was differently clustered and was distantly located in the PCA space. In addition, tribocorrosion induced an increase in the percentage of upregulated and downregulated proteins in the macrophage proteome, revealing that disruption of the covalent nanocoating impacts the macrophage proteome. Although macrophage inflammation induced by tribocorrosion of HA/GO/CoCr surfaces is observed, it is ameliorated by the covalently grafting of HA, which provides immunomodulation by eliciting downregulations in characteristic pro-inflammatory signaling involved in inflammation and aseptic loosening of CoCr joint arthroplasties. Cova

The main aim of this work is to explore the possibility that cold dark matter (CDM) and early dark energy (EDE) can be described by canonical scalar fields that are coupled at the level of its conservation equations. The formalism covers dynamical aspects at the background and linear perturbation levels for an arbitrary coupling function, followed by an example of it. We emphasize the impact of this model on the Matter Power Spectrum and the Cosmic Microwave Background (CMB) spectra, with or without direct interaction. Our findings indicate that the presence of a scalar field can partially counteract the known effects of the other, opening the possibility to avoid some undesired aspects, such as the increase in $\Omega_{m}$ that usually is needed in the case of a purely EDE scalar field scenario, in order to fit the CMB spectra. This opens up the possibility to analyzing whether the interaction can help to ameliorate the cosmological tensions., Comment: 14 pages, 10 figures