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The most severe form of muscular dystrophy (MD), known as Duchenne MD (DMD), remains an incurable disease, hence the ongoing efforts to develop supportive therapies. The dysregulation of autophagy, a degradative yet protective mechanism activated when tissues are under severe and prolonged stress, is critically involved in DMD. Treatments that harness autophagic capacities therefore represent a promising therapeutic approach. Osmolytes are protective organic molecules that regulate osmotic pressure and cellular homeostasis and may support tissue-repairing autophagy. We therefore explored the effects of the osmolyte ectoine in the standard mouse model of DMD, the mdx, focusing on the autophagy-related proteome. Mice were treated with ectoine in their drinking water (150 mg/kg) or through daily intraperitoneal injection (177 mg/kg) until they were 5.5 weeks old. Hind limb muscles were dissected, and samples were prepared for Western blotting for protein quantification and for immunofluorescence for an evaluation of tissue distribution. We report changes in the protein levels of autophagy-related 5 (ATG5), Ser366-phosphorylated sequestosome 1 (SQSTM1), heat shock protein 70 (HSP70), activated microtubule-associated protein 1A/1B-light chain 3 (LC3 II) and mammalian target of rapamycin (mTOR). Most importantly, ectoine significantly improved the balance between LC3 II and SQSTM1 levels in mdx gastrocnemius muscle, and LC3 II immunostaining was most pronounced in muscle fibers of the tibialis anterior from treated mdx. These findings lend support for the further investigation of ectoine as a potential therapeutic intervention for DMD. [ABSTRACT FROM AUTHOR]

Farmers are increasingly faced with challenges such as climate change, population growth, and the need for sustainable food production, while simultaneously having to address the environmental impacts of conventional agriculture. Agroecology has emerged as a holistic and sustainable approach to agriculture, integrating environmental, social, and economic principles. This study investigates the role of digital tools, including decision support systems (DSSs), in supporting agroecological transitions. Through a literature review and analysis of case studies, this paper examines the benefits and challenges associated with the adoption of digital tools in agroecology, highlighting their potential to promote sustainable practices such as soil and water management, pest control, and efficient resource use. The findings indicate that while digital solutions offer significant potential to enhance productivity and improve environmental outcomes, their adoption remains limited due to barriers such as low digital literacy, lack of infrastructure, and concerns about effectiveness in real-world farming conditions. Despite these challenges, digital solutions offer significant potential to enhance productivity, improve environmental outcomes, and support farmers' decision-making. To comprehensively understand their benefits, a holistic approach is necessary, combining digital tools with hands-on training, policy support, and ongoing research. This paper highlights the role of digital tools in agroecology, explores their benefits and challenges, and discusses the need for continued research to assess their long-term potential in terms of the agroecological transition. [ABSTRACT FROM AUTHOR]

Metabolic and bariatric surgery (MBS) is an effective intervention for patients with severe obesity and metabolic comorbidities, particularly when non-surgical weight loss methods prove insufficient. MBS has shown significant potential for improving quality of life and metabolic health outcomes in individuals with obesity, yet it carries inherent risks. Although these procedures offer a multifaceted approach to obesity treatment and its clinical advantages are well-documented, the limited understanding of its long-term outcomes and the role of multidisciplinary care pose challenges. With an emphasis on quality-of-life enhancements and the handling of postoperative difficulties, the present narrative review seeks to compile the most recent findings on MBS while emphasizing the value of an integrated approach to maximize patient outcomes. Effective MBS and patients' management require a collaborative team approach, involving surgeons, dietitians, psychologists, pharmacists, and other healthcare providers to address not only physiological but also psychosocial patient needs. Comparative studies demonstrate the efficacy of various MBS methods, including Roux-en-Y gastric bypass and laparoscopic sleeve gastrectomy that may considerably decrease morbidity and mortality in individuals with obesity. Future studies should target long-term patient treatment, and decision making should be aided by knowledge of obesity, comorbidity recurrence rates, and permanence of benefits. [ABSTRACT FROM AUTHOR]

This study presents a prototype non-aqueous redox flow battery that advances the capabilities of conventional systems by achieving a wide operational voltage range, high efficiency, and prolonged cycle life. Leveraging the redox pair 10-[2-(2-methoxy ethoxy)ethyl]-10H-phenothiazine and 2-ethylterephthalonitrile, the system delivers a discharge cell voltage ranging from approximately 2.25 V to 1.9 V. To address the economic challenges associated with non-aqueous redox flow batteries, this work explores a cost-efficient design using a symmetric cell architecture and a low-cost, porous separator. To evaluate the feasibility and scalability of this approach, a 2D time-transient reactive transport model is developed, integrating Nernst–Planck electroneutrality principles and porous electrode kinetics. The model is optimized and validated against experimental charge/discharge cycles, accurately predicting voltage behavior. Additionally, the study provides crucial insights into the crossover phenomenon, elucidating the transport dynamics and spatial distribution of active species within the cell. This comprehensive framework establishes a robust foundation for future efforts to scale and optimize non-aqueous redox flow batteries for large-scale energy storage applications, bringing them closer to commercial viability. [ABSTRACT FROM AUTHOR]

Background/Objectives: The use of silver diamine fluoride (SDF) has increased in recent years for the management of caries lesions in children and adults. The aim of this study is to determine the level of knowledge and the attitude of Spanish dentists (GDPs) and final-year dental students (DSs) regarding the use of SDF. Methods: A cross-sectional survey (questionnaire) was carried out aimed at final-year dental students (DSs) (n = 43) and registered dentists (GDPs) (n = 1050) in the autonomous community of the region of Murcia (Spain). Results: the response rates were GDPs 7.7% (n = 81) and DSs 84.5% (n = 38). Only 20.98% of GDP respondents reported having been trained on SDF versus 100% of DSs. Significant differences were observed between the groups (p < 0.05). While 94.7% of the students were aware of the indications for the use of SDF, only 56.8% of the general dentists reported it. Similarly, for hypersensitivity treatment, 71.1% of the students were informed versus 40.7% of the general dentists, and indications for paediatric patients, 100% for the DS group and 59.3% in GDPs. In adult patients, indications vary from GDPs' (50%) to DSs' (25.9%) responses. About 94.7% of DSs know the advantages of use and only 50.6% of GDPs. Both groups showed reluctance to use SDF in esthetic zones, with greater acceptance in non-esthetic areas (p < 0.05). In practice, fewer GDPs (27.16%) and DSs (23.68%) had applied SDF, reflecting a gap between knowledge and implementation. Conclusions: Dental students had a significantly higher level of knowledge, a situation that evidences the high level of education and training in the curricular guides of the universities. [ABSTRACT FROM AUTHOR]

Since their origin, sauropodomorphs have undergone numerous anatomical changes from small and bipedal early sauropodomorphs towards massive-bodied and quadrupedal sauropods. However, the timing of these changes in the evolution of the group is unclear. Here, we describe the appendicular skeleton of the early diverging eusauropod Bagualia alba from the late Early Jurassic of Patagonia, Argentina, and conduct a morphological disparity analysis based on a phylogenetic dataset of Sauropoda. The results reveal a change in morphospace occupation between the pre-Toarcian and Toarcian-Middle Jurassic sauropodomorphs and between the latter and Late Jurassic forms. The first shift corresponds with the extinction of non-sauropodan sauropodomorphs and the diversification of sauropods, while the second corresponds with the diversification of Neosauropoda and closely related eusauropods (mamenchisaurids, turiasaurians) in the Late Jurassic, leading to a substantial shift and increase in morphological disparity. Finally, we found that body mass is significantly correlated with the first principal coordinate axis of the morphospace in two-thirds of a random sample of optimal trees, which suggests that body size played a role in shaping the evolution of sauropod morphology. In this context, Bagualia provides insights into the evolution of Sauropoda, particularly regarding changes that occurred during the Early to Middle Jurassic., (© 2025 Willi Hennig Society.)