In this study, we have stated the green biosynthesis of gold nanoparticles (AuNPs) by utilizing the extract of Monstera deliciosa leaves (MDL) as a reducing agent. Biosynthesized flat, thin, and single-crystalline gold nanotriangles obtained through centrifugation are then analyzed by different characterization techniques. The UV - visible absorption spectra of AuNPs exhibited maxima bands in the range of 500-590 nm, indicating a characteristic of AuNPs. XRD analysis revealed the formation of the (111)-oriented face-centered cubic (FCC) phase of AuNPs. ATR-IR spectra showed signatures of stretching vibrations of O-H, C-H, C=C, C=O, C-O, and C-N, accompanied by CH 3 rocking vibrations present in functional groups of biomolecules. FESEM images confirmed spherical nanoparticles with an average diameter in the range of 53-66 nm and predominantly triangular morphology of synthesized AuNPs within the size range of 420-800 nm. NMR, GC-MS, and HR-MS studies showed the presence of different biomolecules, including phenols, flavonoids, and antioxidants in MDL extracts, which play a crucial role of both, reducing as well as stabilizing and capping agents to form stable AuNPs by a bottom-up approach. They were then investigated for their antibacterial assay against Gram-positive ( S. aureus, B. subtilis ) and Gram-negative ( E. coli, P. aeruginosa ) microorganisms, along with testing of antifungal potential against various fungi ( Penicillium sp., Aspergillus flavus, Fusarium oxysporum, Rhizoctonia solani ) using the well diffusion method. Here, biosynthesized AuNPs showed non-antimicrobial properties against all four used bacteria and fungi, showing their suitability as a contender for biomedical applications in drug delivery ascribed to their inert and biocompatible nature., Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-023-03898-0., (© King Abdulaziz City for Science and Technology 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.)