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In viviparous black rockfish (Sebastes schlegelii), the kidney of reproductive-phase males actively produces lipocalin-type prostaglandin D 2 synthase homolog (LPGDSh) protein, which is presumably involved in inter-sexual communication when emitted in the urine. The present study was undertaken to discover whether androgens and their nuclear receptors (Ars) are engaged in regulation of renal LPGDSh protein synthesis in black rockfish. Quantitative real-time polymerase chain reaction, in conjunction with immunohistochemistry and highly sensitive enzyme-linked immunosorbent assay, revealed that intra-abdominal administration of a synthetic androgen, 17α-methyltestosterone (MT), to juvenile black rockfish induced their renal expression of LPGDSh transcript and protein. In situ hybridization visualized arα and arβ transcripts in the renal tubules of mature males during the copulation season, where they were co-localized with LPGDSh protein. Androgens, such as 11β-hydroxytestosterone, MT, dihydrotestosterone, 11-ketotestosterone (11KT), testosterone, and androstenedione transactivated a luciferase reporter vector containing four repeats of a consensus androgen response element (ARE) in the presence of black rockfish Ars (either Arα or Arβ), with differences in ligand-preference and dose-response profiles being observed between the two Ars. In the presence of 11KT, the Ars transactivated a reporter vector containing the proximal 5'-flanking region of an LPGDSh gene in luciferase reporter assays. The region between 2100 bp and 1110 bp upstream from the start codon of the LPGDSh gene, wherein many ARE-like motifs are densely distributed, was imperative for the androgenic transactivation response of the 5'-flanking region. Collectively, these observations verify that renal synthesis of LPGDSh protein is upregulated by androgens., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

The sea lice Caligus elongatus and Lepeophtheirus salmonis are both causing problems in salmonid aquaculture. Since the salmonid specialist L. salmonis represents the dominating problem, research on host-parasite interactions has focused on L. salmonis and Atlantic salmon (Salmo salar), while less is known for the generalist C. elongatus. As new knowledge can be found in the comparison between a specialist and a generalist, the present study compares the salmon immune responses and louse modulatory proteins between C. elongatus and L. salmonis. While the severity of skin lesions inflicted underneath both lice species appeared similar, C. elongatus seemed to be better at dampening inflammatory responses than L. salmonis. A comparison of exocrine gland genes encoding proteins with known effect at the host-parasite interface showed that C. elongatus express most of the genes previously identified in L. salmonis. Interestingly, three orthologues of the labial gland protein 3 (LGP3) known to induce cell death in salmonid immune cells were found. This expansion of the LGP3 gene might explain the limited influx of immune cells observed underneath C. elongatus, though yet unknown C. elongatus specific glandular proteins might also be at play. Despite the limited inflammatory response induced by adult C. elongatus, they provoke a forceful host anti-lice behaviour that is comparably less prominent in salmon infested with L. salmonis. Setule-like processes identified on the ventral surface of the C. elongatus marginal membrane might be of importance here, as could species specific behavioural differences or differences in the host modulatory proteins., (Copyright © 2025 The Authors. Published by Elsevier Ltd.. All rights reserved.)