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Introduction: Autoimmune diseases are heterogeneous and often lack specific or sensitive diagnostic tests. Increased percentages of CD4 + CXCR5 + PD1 + circulating T follicular helper (cTfh) cells and skewed distributions of cTfh subtypes have been associated with autoimmunity. However, cTfh cell percentages can normalize with immunomodulatory treatment despite persistent disease activity, indicating the need for identifying additional cellular and/or serologic features correlating with autoimmunity., Methods: The cohort included 50 controls and 56 patients with autoimmune cytopenias, gastrointestinal, pulmonary, and/or neurologic autoimmune disease. Flow cytometry was used to measure CD4 + CXCR5 + T cell subsets expressing the chemokine receptors CXCR3 and/or CCR6: CXCR3 + CCR6 - Type 1, CXCR3 - CCR6 - Type 2, CXCR3 + CCR6 + Type 1/17, and CXCR3 - CCR6 + Type 17 T cells. IgG and IgA autoantibodies were quantified using a microarray featuring 1616 full-length, conformationally intact protein antigens. The 97.5 th percentile in the control cohort defined normal limits for T cell subset percentages and total number (burden) of autoantibodies., Results: This study focused on CD4 + CXCR5 + T cells because CXCR5 upregulation occurs after cognate T-B cell interactions characteristic of autoimmune diseases. We refer to these cells as circulating T follicular memory (cTfm) cells to acknowledge the dynamic nature of antigen-experienced CXCR5 + T cells, which encompass progenitors of cTfh or Tfh cells as well as early effector memory T cells that have not yet lost CXCR5. Compared to controls, 57.1% of patients had increased CXCR5 + CXCR3 + CCR6 + cTfm1/17 and 25% had increased CXCR5 + CXCR3 - CCR6 + cTfm17 cell percentages. Patients had significantly more diverse IgG and IgA autoantibodies than controls and 44.6% had an increased burden of autoantibodies of either isotype. Unsupervised autoantibody clustering identified three clusters of patients with IgG autoantibody profiles distinct from those of controls, enriched for patients with active autoimmunity and monogenic diseases. An increased percentage of cTfm17 cells was most closely associated with an increased burden of high-titer IgG and IgA autoantibodies. A composite measure integrating increased cTfm1/17, cTfm17, and high-titer IgG and/or IgA autoantibodies had 91.1% sensitivity and 90.9% specificity for identifying patients with autoimmunity. Percentages of cTfm1/17 and cTfm17 percentages and numbers of high-titer autoantibodies in patients receiving immunomodulatory treatment did not differ from those in untreated patients, thus suggesting that measurements of cTfm can complement measurements of other cellular markers affected by treatment., Conclusions: This study highlights two new approaches for assessing autoimmunity: measuring CD4 + CXCR5 + cTfm subsets as well as total burden of autoantibodies. Our findings suggest that these approaches are particularly relevant to patients with rare autoimmune disorders for whom target antigens and prognosis are often unknown., Competing Interests: Conflicts of Interest: EMH, SC, LS, SS, CY, AN, MG-A, CDP, BL, JC have no conflicts of interest to disclose. DHK is a consultant for Adivo Associates. RFG receives research funding from Novartis, Sobi, and Agios and is a consultant for Agios and Sanofi. JP is an employee of Sengenics. LAH received salary support from the Childhood Arthritis and Rheumatology Research Alliance, investigator-initiated research grants from Bristol Myers Squibb, and consulting fees from Sobi.