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A polyphasic approach was applied to characterize taxonomically a novel endophytic bacterial strain, designated as EP178 T , which was previously isolated from Passiflora incarnata leaves and characterized as plant-growth promoter. The strain EP178 T forms Gram stain-negative and rod-shaped cells, and circular and yellow-pigmented colonies. Its growth occurs at 10-37 °C, at pH 6.0-8.0, and tolerates up to 7% (w/v) NaCl. The major cellular fatty acids found were summed feature 8 (C 18:1 ω7c), summed feature 3 (C 16:1 ω6c /C 16:1 ω7c), and C 16:0 , and the predominant ubiquinone was Q-9. The phylogenetic and nucleotide-similarity analysis with 16S rRNA gene sequences showed that strain EP178 T belongs to Pseudomonas genus. The genomic-based G + C content was 65.5%. The average nucleotide identity and digital DNA-DNA hybridization values between strains EP178 T and the closest type strain, P. oryzihabitans DSM 6835 T , were 92.6% and 52.2%, respectively. Various genes associated with plant-growth promoting mechanisms were annotated from genome sequences. Based on the phenotypic, genomic, phylogeny and chemotaxonomic data, strain EP178 T represents a new species of the genus Pseudomonas, for which the name Pseudomonas flavocrustae sp. nov. was proposed. The type strain is EP178 T (= CBMAI 2609 T  = ICMP 24844 T  = MUM 23.01 T )., (© 2024. The Author(s).)