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Agriculture is a major contributor to greenhouse gas emissions and is vulnerable to climate change. Regenerative agriculture, which focuses on increasing soil carbon, has been recognized as a potential climate solution. A recent study found that seven regenerative practices, including planting cover crops and using non-chemical fertilizers, all increased carbon sequestration. However, the study also highlights that regenerative agriculture has other benefits beyond carbon sequestration, and the focus should not solely be on carbon credits. The popularity of regenerative agriculture is growing, but there is a risk of it being co-opted and losing its essence. [Extracted from the article]

Spirotetramat is a frequently used insecticide in integrated pest management (IPM) strategies against rosy apple aphid (Dysaphis plantaginea) and woolly apple aphid (Eriosoma lanigerum) in apple cultivation. It is known that paraffin oil adjuvants increase the effect of spirotetramat against aphids. In contrast, there is a knowledge gap regarding the effects of co-applied paraffin (mineral or petroleum) oil on the degradation of spirotetramat and its metabolites (B-enol, B-keto, B-mono, and B-glu), which has not been previously investigated. Spirotetramat combined with formulated paraffin oil was tested against two aphid species and the residues of spirotetramat and its metabolites on leaves and apples were quantified using Liquid Chromatography coupled with tandem Mass Spectrometry (LC-MS/MS). The results showed that spirotetramat is highly effective against D. plantaginea, decreasing infestation by almost 100%. Furthermore, spirotetramat was shown to be effective against E. lanigerum, the reduction in infestation ranged between 67.9 and 97.7% during the last validation date. The addition of paraffin oil increased the effect of spirotetramat and affected its degradation, indicating that its efficacy could be connected to its metabolites. B-mono and B-glu were the most persistent metabolites in apple fruit overall when paraffin oil was applied. [ABSTRACT FROM AUTHOR]

Agroecology has recently become an alternative production model that tries to face the negative environmental and socioeconomic consequences of the agricultural model based on excessive agrochemical use and no-tillage practices. It searches for alternatives to pest management so as to increase yields while promoting biodiversity conservation. Our goal was to know the perception and opinion of agroecological farmers of the Pampa region on crop pests, the techniques they use for pest management and their perceptions on biodiversity. A survey was conducted which provided 103 responses. The main pest mentioned was ants. Of farmers surveyed, 64.71% claim that their main pest significantly reduces their yields, 79.42% claim that they monitor their crops for pests, although those with over 25 years of experience tend not to. Farmers have a mostly positive perception of biodiversity, particularly birds even though many identify pigeons and parrots as damaging. These results make it possible to describe the agroecological Pampa farmers and to show some common pest issues that need to be observed to benefit production in the area. The study highlights the importance of biodiversity for these farmers and their willingness to use tools that attract birds that could potentially manage pests. [ABSTRACT FROM AUTHOR]

Agroecology, as a holistic approach to sustainable food systems, is gaining momentum globally as a key approach to addressing current challenges in agricultural and food production. In sub-Saharan Africa, despite numerous efforts to address declining soil productivity, water scarcity, and increasing pest pressure through agroecological soil, water, and integrated pest management (IPM) practices, the adoption of such practices remains low. As part of the CGIAR Agroecology Initiative, we conducted a collaborative rapid innovation assessment of existing soil, water, and pest management practices in two Agroecological Living Landscapes (ALLs) in Makueni and Kiambu counties, Kenya. The assessment also included an evaluation of the performance of these practices and identified farmer preferences. Using a multi-stage approach, we applied stratified random sampling to identify 80 farmers for farm assessments and in-depth interviews. A total of 31 practices were identified, of which 26 were further evaluated. The evaluation revealed a heterogeneous set of socio-economic and biophysical contextual factors influencing practice performance. Respondents identified 19 strengths, and 13 challenges associated with the practices, highlighting opportunities for innovation to improve or adapt performance. Farmers also expressed preferences for future adoption of 31 practices, 77% of which were listed in one of the three focus areas, namely soil management, water management, or IPM. The other 33% were associated with multiple functions and were listed under two or three of the focus areas. The results of the collaborative assessment informed a broader co-design cycle that included participatory prioritization and selection of innovative practices, experimental design, and monitoring protocols. This collaborative and systematic approach was taken because innovative practices often fail to be adopted due to a lack of co-design and inclusion of local perspectives in innovation design, and a disconnect between science and practice. Our study highlights the importance of integrating stakeholder input and transdisciplinary technical expertise in the co-design and implementation of agroecological innovations. It also emphasizes the importance of using a structured methodology to understand farmers' options, context, and preferences while co-designing locally relevant agroecological practices, which promotes holistic and inclusive adoption, successful implementation and long-term sustainability of agroecological practices. [ABSTRACT FROM AUTHOR]

Introduction: Phenacoccus solenopsis Tinsley, poses a significant threat to a range of crops worldwide. This study aimed to evaluate the efficacy of entomopathogenic fungi (Alternaria murispora and Alternaria destruens) and bacteria (Streptomyces bellus-E23-2) against adult females of P. solenopsis under laboratory (26 ± 2°C) and greenhouse conditions. Methods: Laboratory trials tested A. murispora, A. destruens (104-1010 conidia mL-1), and S. bellus E23-2 (104-1010 cfu mL-1), alone and in combination, recording mortality rates and LC50 values. Greenhouse trials tested the best lab treatments on infested potato plants, monitoring pest density and plant quality. Results and discussion: In laboratory trials, A. murispora at 1010 conidia mL-1 was the most effective, achieved 79.7% mortality (LC50 = 1.338 x 108 conidia mL-1 after 14 days). Combination treatments significantly enhanced efficacy, with A. murispora + S. bellus E23-2 (1010 conidia mL-1 + 1010 cfu mL-1) reaching 85.3% mortality. In greenhouse trials, the combination treatments notably reduced P. solenopsis densities and increased the number of infected mealybugs, with A. murispora + S. bellus E23-2 being the most effective. These treatments did not harm plant quality, unlike imidacloprid, which reduced visual quality despite its high efficacy. Alternaria murispora and S. bellus E23-2 effectively control P. solenopsis, providing a sustainable, plant-safe alternative to chemical insecticides. [ABSTRACT FROM AUTHOR]

Pest detection and control is very essential in greenhouse crop production. Image segmentation is one of the most desirable procedures for identifying the pest from the image. However, manually detecting pests results in lower accuracy levels and requires prolonged crop monitoring. In the existing techniques, several limitations take place such as low efficiency, high storage requirements, and limited region-level accuracy. Furthermore, it is challenging to gather a high-quality dataset that contains a variety of healthy and diseased leaf types, as well as distinct pest infestations. In order to overcome these issues, a novel Radial Bi-symmetric Divergence RCNN-based Segmentation method is proposed to effectively detect and classify leaf-based pest detection, which provides good efficiency and good storage requirements. In order to overcome this issue a novel Threshold-based Graded Mask RCNN is introduced, which is the new convolutional neural network utilized to obtain various types of healthy and diseased leaves. In the existing pest detection techniques, mislabeled data leads to erroneous learning and reduces its effectiveness in identifying pests. Hence, the proposed pest detection technique is tested using an optimized approach with a feature set. As a result, the proposed mechanism is designed for the training and testing of a plant disease dataset, which is more accurate and reliable for identifying the pest. This experiment revealed that the accuracy level increased with high efficiency in the proposed technique when compared to the existing ones by a significant margin. [ABSTRACT FROM AUTHOR]

Soilless culture refers to the method of cultivating plants without soil as a growing medium, and the use of hydrogel in soilless culture overcomes many technical difficulties, but there are many applications that just stay at the experimental level, with fewer applications in fields. Although many new functionalized hydrogels may help to overcome the shortcomings of traditional media and give them more functionality, little research on hydrogels over the past decades has been reflected in soilless culture techniques. This paper details the concept and development of soilless culture, as well as several applications of hydrogels in soilless culture, including the use of hydrogels as soilless culture substrates and the application of hydrogel membranes. The important roles of different types of hydrogels in soilless culture, such as antimicrobial properties, salt tolerance and high water absorption, are highlighted, and these excellent properties largely overcome a series of drawbacks of traditional cultivation methods. The application of hydrogels in pest control is also discussed. Hydrogel applications to soilless culture have better prospects for the creation of more promising multifunctional composite hydrogel membranes and hydrogel substrates capable of supporting plants throughout their growth cycle. [ABSTRACT FROM AUTHOR]

Insects attach to various surfaces that differ, among others, in roughness and wettability. Identifying surface characteristics that allow or prevent insects from attaching are an important research avenue of pest control. Here we take an experimental approach to analyse the attachment of common bed bugs, Cimex lectularius Linnaeus (1758), to Perspex (PMMA) substrates. We construct a reliable centrifuge device that allows the measurement of attachment forces at substrate roughnesses, Ra, between 0.02 and 1.3 μm and at two wettabilities. Our results suggest that bed bug attachment to surfaces is minimal at a substrate roughness of 0.2 and 0.4 μm on normal PMMA, where the lowest attachment force was 0.8 mN and the safety factor 15. At lower and higher roughness, attachment forces were higher and the safety factor increased to a maximum of 133. On PMMA that was made superhydrophobic by spray‐coating, attachment was lowest (0.2 mN) at the lowest roughness and continuously increased with increasing roughness, reaching 2.5 mN and a safety factor of 46. For every roughness, attachment forces were lower on superhydrophobic than on normal PMMA. This knowledge may inspire the development of repelling substrates for bed bug control. [ABSTRACT FROM AUTHOR]

AbstractTomato is one of the most widely produced vegetables in Ethiopia. Year-round production, mono-cropping, and genetically uniform hybrid varieties in relatively large areas result in pest buildup and recurrent pest epidemics in the country. Chemical control remains the primary management option for controlling tomato pests, and frequently indiscriminate and injudicious use of pesticides leads to several problem such as resistance development in insects/pathogens, destruction of natural enemies, food safety risks due to pesticide residues on the edible products and destruction of pollinators resulting in the non-setting of fruits and low yields. Multi-location and multi-season trials were conducted at Rift-Valley tomato production sites to reduce synthetic pesticides and promote good agricultural practice (GAP) packages. GAP package composed of healthy seedlings produced using seedling trays and treated with Trichoderma asperellum, and scheduled spray of bio-pesticides such as Beauveria bassiana, Lecanicillium lecanii, Metarhizium anisopliae, and neem extract depending on the insect pests at early infestation time, along with the installation of colored sticky traps (yellow and blue) significantly reduced Phthorimaea (Tuta) absoluta and increased marketable yield compared to the farmers’ practice and untreated control. Hence, the GAP package can be considered effective and economical in managing tomato pests. [ABSTRACT FROM AUTHOR]

Cool season legumes (Faba bean, chickpea, lentil, pea, and grass pea) are important protein harvests for food and nutrition security in many countries. They play key roles in sustainable cereal production through their ecological benefits. However, diseases and pests attack continue to have a substantial impact on crop yield and quality. Although growers used different control options to manage these biotic stresses such as pesticide application, cultural practices, and resistant varieties, there is a pressing need for the development of new, more cost-effective and environmentally friendly solution to help farmers in facing the existing environmental issues. Recently, there is a growing interest among researchers in exploiting Volatile Organic Compounds (VOCs) for the elaboration of disease and pest control strategies in food legumes and other crops. These compounds have important functions in ecological relationships occurring between plants and their surrounding environment, as well as plants and others species, such as pests and pathogens. Due to their unique properties, VOCs can be employed in improving management alternatives for food legume diseases and pests. In this assessment, we investigated the role of VOCs in plantpest and plant-pathogen interactions and their present applications in pest and diseases control strategies. We emphasized the ecological importance of employing plant VOCs in legume farming and crop breeding. Additionally, we highlighted the potential of microbial VOCs in facilitating microbe-microbe, microbe-plant and microbe-plant-pest interactions, along with their role in food legume protection. [ABSTRACT FROM AUTHOR]

Capsicum pepper is a rich source of phytochemical compounds such as capsaicinoids, phenols, flavonoids, and so forth. Due to their antimicrobial and antioxidant potential all of these compounds have been assessed and used for both human and plant health benefits. Herein, three fresh varieties of Capsicum annuum (Cacho de Cabra, Bell pepper, and Hungarian Wax varieties) and one fresh and ripe variety of C. baccatum (Cristal) were evaluated. Capsaicin, dihydrocapsaicin, nordihydrocapsaicin and the phenolic content of Capsicum spp. extracts were characterised. The antifungal potential of capsaicinoids and antioxidant activities, and the ecotoxicity of each Capsicum spp. extract, using the model Galleria mellonella, were also evaluated. Phytochemical analyses showed that the Cristal and Hungarian Wax varieties presented the highest amount of capsaicin, dihydrocapsaicin, and nordihydrocapsaicin; while Bell Pepper had the highest phenol content and antioxidant activity. Capsaicinoids' standards and Capsicum spp. extracts showed fungistatic activity against the fungal strains assessed. For the fungal strains assessed, the fungistatic activities of capsaicinoids' standards were higher than those observed in Capsicum spp. extracts. The Hungarian Wax extracts inhibited slightly the growth of Aspergillus niger MUM05.11 and Fusarium oxysporum MUM16.143. Similarly, A. niger, F. oxysporum, Rhizopus arrhizus MUM16.05 and Alternaria sp. UFRO17.178 had their growth retarded by the use of Cacho de Cabra and Cristal extracts. Noticeable changes were observed in the fungal strains' morphologies, such as the presence of fragile fungal structures, pigmentation loss, variation in the reproductive structures size and the conidia number. Capsicum extracts weaken the growth of fungi, indicating their fungistatic potential. Considering the fungistatic potential and non-ecotoxicity of these extracts, it is possible to suggest their use as a tool for pest management in the agri-food sector, controlling the growth and reproduction of fungi without posing a risk to nontarget biodiversity. [ABSTRACT FROM AUTHOR]

Pest‐regulating natural enemies often require crop and non‐crop habitats to persist and be effective. These habitats offer hibernation sites, alternative prey or floral resources but can also be complementary in when they provide resources. The relative contribution of these different habitats to the performance of pest‐regulating insects is largely unknown, since it is difficult to study empirically. Landscape‐based population‐dynamical modelling can fill this research gap. In this study, we modelled the dynamics of predatory hoverflies (Diptera: Syrphinae) and their aphid prey in a collection of habitats. These hoverflies are dependent on different habitats for different reasons. While their larvae predate on aphids, adult hoverflies require floral resources. Resources are often segregated in different habitats. In addition, they are only temporarily available in any habitat type. In our model, hoverflies move between different habitats and select where to feed and where to produce off‐spring based on optimal foraging considerations. The model represents habitats common in arable landscapes and their temporal availability of resources is parameterized based on field observations. The model shows that effective pest suppression will only occur in the presence of at least three different habitats, including two crops that provide prey resources at different times of the year. A woody habitat with shrubs and trees provide aphid prey very early and late in the season, whereas an early crop and a late crop cover the period in between. In addition, these habitats need to provide floral resources in the same period as aphids, for example by flower strips in the margins of crop fields or blossoming trees or shrubs in the woody habitat. Under the model assumptions, the best pest suppression is obtained when the woody habitats take up 6% to 10% of the landscape. In addition to yearly population fluctuations, also bi‐ or multi‐yearly cycles may occur, depending on the composition of the landscape. Synthesis and applications. Our model shows when an agricultural landscape is ‘complete’ for natural enemies (and for effective pest control). For this, it needs to contain multiple habitats that supply floral and prey resources in different periods of the year. [ABSTRACT FROM AUTHOR]

Cover crops are multifunctional tools that mitigate environmental impacts of agriculture, enhance resilience to weather extremes and suppress weeds and arthropod pests. Cover crops provide non‐crop food and habitat resources that attract natural enemies of pests, but their outcomes for pest management are less clear in regions where keystone mutualisms between red imported fire ants and aphids dominate. Here, we manipulate ant exclusion treatments and cover crop treatments (living mulches and terminated cover crops) that vary in food/habitat resources and examine responses of ants, aphids, other herbivores and predators in a cotton agroecosystem. Living mulches reduced both ants and aphids in the crop canopy by 97% and 93%, respectively, relative to bare soil treatments, and terminated cover crops reduced them as well by a lesser degree (~50%). Non‐aphid herbivores occurred in low densities system‐wide and increased in living mulches, whilst native predators had variable responses to cover crops and ant exclusion. Cover crops had no effect on prey removal in the crop canopy, but living mulches tripled rates of weed seed biocontrol, relative to bare soils. Cover crops elicited a shift in fire ant foraging from cotton foliage downward to the soil‐surface, preventing competitive exclusion by keystone ant/aphid mutualists that dominate crop monocultures. Cover crops altered the system‐wide impacts of fire ants: reducing ecosystem disservices (i.e. aphid tending) and enhancing ecosystem services (i.e. weed seed biocontrol). These results provide incentives for cover crop adoption as a regenerative practice in large‐scale commercial agriculture. [ABSTRACT FROM AUTHOR]

Field surveys were conducted on golf courses reporting the inability of imidacloprid to control white grubs (Coleoptera: Scarabaeidae) when applied preventively. Surveys of five sites with significant past imidacloprid use (>10 years) revealed significantly greater white grub populations in rough‐mown turf following imidacloprid treatment than that of adjacent short‐mown fairways. Additionally, spatial analysis by distance indicEs (SADIE) analyses demonstrated a positive correlation between white grub and thatch spatial patterns. To investigate the impact of thatch on imidacloprid efficacy and translocation throughout the turfgrass plant, greenhouse experiments were conducted using turf with differing thatch levels. Imidacloprid concentrations in soil and plant tissues were measured with high‐performance liquid chromatography (HPLC) and compared to values obtained through an enzyme‐linked immunosorbent assay (ELISA) kit to determine if the latter could be a cost‐effective alternative in future studies. ELISA provided reliable estimates of concentrations of imidacloprid compared to HPLC, with only minor discrepancies noted across different types of treatments and assessment timings. Despite finding higher imidacloprid levels in leaf tissues compared to roots and some differences in concentration across thatch treatments, there was no clear pattern showing that thatch thickness significantly affects imidacloprid penetration or accumulation in plant tissues or soil over time. These findings suggest that factors other than thatch thickness may contribute to the observed field failures of imidacloprid in controlling white grubs. Further research is necessary to identify these factors and optimize the use of imidacloprid in turfgrass pest management strategies. [ABSTRACT FROM AUTHOR]

Background: Entomopathogenic fungal infection-induced dysbiosis of host microbiota offers a window into understanding the complex interactions between pathogenic fungi and host symbionts. Such insights are critical for enhancing the efficacy of mycoinsecticides. However, the utilization of these interactions in pest control remains largely unexplored. Results: Here, we found that infection by the host-specialist fungus Metarhizium acridum alters the composition of the symbiotic microbiota and increases the dominance of some bacterial symbionts in locusts. Meanwhile, M. acridum also effectively limits the overgrowth of the predominant bacteria. Comparative transcriptomic screening revealed that the fungus upregulates the production of MaCFEM1, an iron-binding protein, in the presence of bacteria. This protein sequesters iron, thereby limiting its availability. Functionally, overexpression of MaCFEM1 in the fungus induces iron deprivation, which significantly suppresses bacterial growth. Conversely, MaCFEM1 knockout relieves the restriction on bacterial iron availability, resulting in iron reallocation. Upon ΔMaCFEM1 infection, some host bacterial symbionts proliferate uncontrollably, turning into opportunistic pathogens and significantly accelerating host death. Conclusions: This study elucidates the critical role of pathogenic fungal-dominated iron allocation in mediating the shift of host microbes from symbiosis to pathogenicity. It also highlights a unique biocontrol strategy that jointly exploits pathogenic fungi and bacterial symbionts to increase host mortality. 5MpJPM8R63G6GM1oNrbXUC Video Abstract [ABSTRACT FROM AUTHOR]

Background: The development of modern agriculture has significantly contributed to improving global food security and safety, alleviating poverty, and enhancing human health and livelihoods. However, the rapid advancement of modern agriculture has also brought about various challenges that limit its sustainable development. This commentary aims to discuss these issues through the One Health lens, and provide valuable insights for balancing modern agricultural activities with the need to protect and promote the health of all the sectors. Main text: This commentary explores the multifaceted impacts of modern agriculture on social development, as well as the associated various health challenges and environmental impacts within the One Health framework. Key issues include ecosystem degradation, increased risk of interspecies disease transmission like zoonoses, reverse zoonoses, and vector-borne diseases, and the escalated threat of antimicrobial resistance due to intensified agricultural production and increased antimicrobial use. To address these challenges, this commentary outlines potential solutions anchored in the development and implementation of modern technologies and good agricultural practices, such as precision farming, integrated pest management, biosecurity measures, vaccination programs, as well as surveillance and early detection of health risks. Conclusions: Good agricultural practices supported by scientific and technological advancements are essential for aligning productivity with the One Health vision, ensuring the health and resilience of all the sectors. Enhancing stakeholder education, strengthening regulatory frameworks, and providing supportive policies and infrastructure for farmers to adopt sustainable practices are crucial for the long-term viability of agrifood systems. The Food and Agriculture Organization of the United Nations plays a pivotal role in guiding this sustainable transformation through the One Health approach. [ABSTRACT FROM AUTHOR]

Ecological theory on predator–prey interactions and an understanding of how environmental factors affect predator dispersal and host finding is foundational to biological control of herbivorous pests in agricultural systems. However, our knowledge about the potential interaction between (A) the individual experience or conditioning of the arthropod predator based on its rearing environment and (B) the environmental conditions at the ultimate release site to control a particular pest is quite limited. We hypothesized that simplified rearing conditions present in commercial insectaries may produce predators that are less adept to navigate complex and challenging crop systems compared to those reared on target crops. We chose Phytoseiulus persimilis (Phytoseiidae) as a case study because it is an important commercialized predator of the twospotted spider mite on diverse crops worldwide. We established colonies of P. persimilis on bean (commercial rearing host plant) and strawberry (target crop plant) for over five generations and compared patterns of ambulatory dispersal when released on bean or strawberry and feeding efficiency on strawberry. Dispersal of P. persimilis was largely predicted by the presence of prey (higher dispersal when prey was absent), followed by the host on which predators were released (higher dispersal on bean compared to strawberry), and lastly by source colony (few examples of a significant difference). We found little evidence of an interaction between the two latter main effects. Rather, they were likely independent and additive. Prey consumption by P. persimilis on strawberry was higher for P. persimilis reared on strawberry compared to bean, but this did not translate into higher rates of oviposition. Synthesis and applications. This study provides insight into sources of variation in dispersal and host finding behaviour of biocontrol agents that may help to predict biocontrol success. To compensate for prey availability and host plant effects on predator dispersal and biocontrol efficacy, we suggest that release rates and strategies should be tailored to host plants given their physical characteristics and prey quality. [ABSTRACT FROM AUTHOR]

Zinnia elegans of Family Asteraceae is a flowering plant grown widely in gardens in Indonesia. The plant is also often integrated into pest management of different agricultural commodities as its colourful flowers may attract beneficial insects including natural enemies. A total of eight viral symptomatic and four non-symptomatic Z. elegans samples were collected from four districts in Kulon Progo and Sleman Regencies of Special Region of Yogyakarta. They were molecularly tested using two universal primer pairs for begomoviruses, and two specific primer pairs for cucumber mosaic virus (CMV, Cucumovirus) detections. The eight symptomatic samples were all positive for CMV but negative for begomoviruses infections. The four non-symptomatic samples were tested negative to begomoviruses and CMV. Partial RNA2 and RNA3 segments of four CMV isolates were sequenced to demonstrate that they are members of subgroup IB. 4Ze-Ngaglik isolate was shown to be mechanically transmitted to healthy Z. elegans and cucumber cv. Baresta (Cucumis sativus). To the best of our knowledge, this report confirmed the first CMV occurrence in Z. elegans in Indonesia. [ABSTRACT FROM AUTHOR]

Plastic waste in agriculture, particularly from polyethylene mulch, poses significant environmental challenges. Synthetic biodegradable mulch has emerged as a sustainable alternative, derived from renewable resources such as thermoplastic starch, polylactic acid, polyhydroxyalkanoates, and copolyesters. This review explores the benefits of synthetic biodegradable mulch, its environmental impact, and the policy landscape to support its adoption. A review of existing literature was conducted, focusing on three aspects: (1) the performance of synthetic biodegradable mulch in crop production and pest control, (2) the environmental, socioeconomic, and climate resilience compared to polyethylene mulch, and (3) the institutional policies that promote synthetic biodegradable mulch adoption. The analysis considered comparative data on yield, pest management, and sustainability metrics. Synthetic biodegradable mulch performs similarly or better than polyethylene mulch in various agricultural practices. It enhances crop yield, quality, and weed suppression, acts as a physical barrier against pests and diseases, reduces chemical usage, and aids in water and nutrient management. Moreover, synthetic biodegradable mulch offers environmental benefits by reducing plastic waste, microplastic pollution, and greenhouse gas emissions, contributing to climate change mitigation. While synthetic biodegradable mulch provides numerous advantages, adoption faces challenges such as high initial costs, farmer preferences, and the regulatory framework. Effective institutional policies and increased consumer demand could drive wider adoption, offering potential for improved livelihoods among small farmers while promoting environmental sustainability. [ABSTRACT FROM AUTHOR]

The piercing-sucking pests can suck the sap of plants. After infestation, plants may become curled, withered, and develop yellowish lesions. Endosymbionts are ubiquitously distributed in piercing-sucking pests and are closely related to their hosts. Mastering various information about endosymbiotic bacteria in piercing sucking pests and utilizing it is crucial for controlling pests and reducing the spread of viruses by pests. This paper reviews the diversity of endosymbiotic bacteria in piercing sucking pests, the relationship between endosymbiotic bacteria and hosts, and the use of endosymbiotic bacteria for pest control, in order to further achieve green pest control in tea gardens through the regulation of piercing sucking pests by endosymbiotic bacteria. [ABSTRACT FROM AUTHOR]

Intensive agriculture often comes at the expense of soil health. A shift towards practices that foster soil health will support yield and defences against pests and diseases. Growing crops in monoculture is the standard in modern agriculture, though strip‐cropping, in which different crops are planted in strips, is a promising strategy in the transition towards sustainable agriculture. Increasing crop diversity is hypothesized to positively influence arbuscular mycorrhizal fungi (AMF), thereby enhancing soil health, but the mechanisms by which AMF‐plant symbioses contribute to higher yields and reduced pest populations in strip‐cropping systems remain unclear. We used a green‐house experiment with potato plants to explore the soil legacy effects of mono‐cropping versus strip‐cropping systems, AMF inoculation and aphid infestation on AMF root colonization, the induction of plant defences (untargeted LCMS‐based metabolomics), aphid population size and potato yield. We found that potato plants grown in strip‐cropping soil had higher AMF colonization than plants grown in mono‐cropping soil. Potato plants grown in strip‐cropping soil also had higher shoot nitrogen content, increased solamargine levels, and reduced aphid populations. AMF root colonization was only enhanced by the addition of commercial AMF in mono‐cropping soils. Potato plant metabolites were affected by strip‐cropping soil, including jasmonic acid (JA) derivatives. Structural equation models revealed that strip‐cropping soil directly reduced aphid populations and also had a negative direct effect on the JA precursor OPC‐8, and hydroxyJA‐glucosides, indicating complex effects of strip‐cropping soils on JA‐inducible plant defences. Indirect benefits of strip‐cropping soil and AMF inoculation on tuber yield were mediated by their direct positive effects on plant nitrogen content. Our results emphasize the potential of strip‐cropping to enhance AMF root colonization in the field. We show that soil legacy effects of strip‐cropping alter the plant metabolome in ways that suppress of aphid populations. Strip‐cropping legacy effects are the result of crop diversity, crop neighbour and edge effects resulting from crop management practices. While the mechanisms by which soil from strip‐cropping supresses pest populations still need to be identified, our study underscores the potential for strip‐cropping to enhance pest control and yield via soil mediated processes. Read the free Plain Language Summary for this article on the Journal blog. [ABSTRACT FROM AUTHOR]

ABSTRACT Plants can perceive and respond to external stimuli by activating both direct and indirect defences against herbivores. Soil‐dwelling entomopathogenic nematodes (EPNs), natural enemies of root‐feeding herbivores, carry symbiotic bacteria that grow and reproduce once inside arthropod hosts. We hypothesized that the metabolites produced by EPN‐infected insect cadavers could be perceived by plants, thereby activating plant defences systemically. We tested this hypothesis by adding three EPN‐infected Galleria mellonella cadavers to maize plants and testing plant responses against a major maize pest (Spodoptera frugiperda) and one of its parasitoids (Trichogramma dendrolimi). We found that S. frugiperda females deposited fewer, and caterpillars fed less on maize plants growing near EPN‐infected cadavers than on control plants. Accordingly, EPN‐infected cadavers triggered the systemic accumulation of defence hormones (SA), genes (PR1), and enzymes (SOD, POD, and CAT) in maize leaves. Furthermore, four volatile organic compounds produced by plants exposed to EPN‐infected cadavers deterred S. frugiperda caterpillars and female adults. However, these compounds were more attractive to T. dendrolimi parasitoids. Our study enhances the understanding of the intricate relationships within the above‐ and belowground ecosystems and provides crucial insights for advancing sustainable pest management strategies. [ABSTRACT FROM AUTHOR]

Ingested toxins formulated as baits can be effective in the management of insect pests, but their efficacy may be influenced by insect foraging alongside active ingredients. There are several components that might influence insect consumption of toxic baits, one of which being prior nutritional exposure. In our study, we investigated how protein deprivation following adult emergence influences lethal and sublethal effects of boric acid on the pest tephritid melon fly, Zeugodacus cucurbitae. We performed a series of experiments to address the impact of prior diet on mortality, diet consumption, enzymes involved in detoxification and antioxidation and fly activity. Newly emerged melon fly adults were provided either diet containing 3:1 sucrose/yeast hyrdrolysate or just sucrose for three days prior to bioassays. Overall, protein deprivation had a strong effect on fly interactions with boric acid. In two separate experiments (continuous exposure and short duration feeding), insects only pre‐fed sucrose had significantly higher mortality compared with those pre‐fed protein. Insects that were pre‐fed only sucrose removed more liquid diet over the same duration of the experiments, suggesting compensatory foraging behaviours. Adults pre‐fed sucrose had higher catalase (1.5×) and superoxide dismutase (1.7×) activities compared with adults pre‐fed sucrose‐yeast, but we found no effect of boric acid on their activity or that of glutathione‐S‐transferase or acetylcholinesterase activity. Boric acid consumption also significantly reduced the activity of melon flies. Overall, our results demonstrate that prior diet in fly adulthood can impact subsequent exposure to and efficacy of ingested toxicants. [ABSTRACT FROM AUTHOR]

Root-knot nematodes (RKNs) are plant pests that infect the roots of host plants. Bacillus thuringiensis (Bt) nematicidal proteins exhibited toxicity to nematodes. However, the application of nematicidal proteins for plant protection is hampered by the lack of effective delivery systems in transgenic plants. In this study, we discovered the accumulation of leucoplasts (root plastids) in galls and RKN-induced giant cells. RKN infection causes the degradation of leucoplasts into small vesicle-like structures, which are responsible for delivering proteins to RKNs, as observed through confocal microscopy and immunoelectron microscopy. We showed that different-sized proteins from leucoplasts could be taken up by Meloidogyne incognita female. To further explore the potential applications of leucoplasts, we introduced the Bt crystal protein Cry5Ba2 into tobacco and tomato leucoplasts by fusing it with a transit peptide. The transgenic plants showed significant resistance to RKNs. Intriguingly, RKN females preferentially took up Cry5Ba2 protein when delivered through plastids rather than the cytosol. The decrease in progeny was positively correlated with the delivery efficiency of the nematicidal protein. In conclusion, this study offers new insights into the feeding behavior of RKNs and their ability to ingest leucoplast proteins, and demonstrates that root leucoplasts can be used for delivering nematicidal proteins, thereby offering a promising approach for nematode control. This study uncovers that root-knot nematodes (RKNs) can absorb proteins from root leucoplasts. Based on this finding, an efficient system using root leucoplasts to deliver Bt nematicidal proteins is developed for controlling RKNs , offering a novel strategy for nematode control. [ABSTRACT FROM AUTHOR]

Intensive agricultural land use negatively impacts biodiversity, including arthropod predator diversity and their pest control potential. Alley‐cropping agroforestry systems (integration of tree rows into arable land) are increasingly considered an economically viable alternative for more sustainable and biodiversity‐friendly agriculture. However, their effectiveness in promoting generalist predator diversity and pest control, especially as a function of space (distance from tree rows) and time (across the growing season) remains poorly understood. We assessed how spider abundance, taxonomic and functional diversity (as proxies of pest control potential) respond to temperate alley‐cropping agroforestry systems as compared to open croplands. Additionally, we analysed whether spiders with different habitat preferences (eurytopic, forest and open‐habitat specialists) show distinct responses. Lastly, we analysed whether the influence of agroforestry changes with decreasing tree proximity and across the growing season. Tree row proximity generally benefited the abundance, as well as taxonomic and functional diversity of the overall spider communities. Alley‐cropping promoted the abundance and taxonomic diversity of forest specialists and eurytopic spiders, without negatively affecting open‐habitat specialists. The positive effect of alley cropping was strongest within tree rows and their immediate vicinity, but was still detectable at considerably farther distances. These patterns were temporally dynamic with spider abundance and taxonomic diversity being highest within tree rows in early spring and spiders spilling over to adjacent crop rows in subsequent periods. Synthesis and applications: Overall, our findings highlight that the inclusion of tree rows benefited spiders across the entirety of the 48 m crop rows, independent of crop type. Since the peak in spider abundance and taxonomic diversity in crop rows coincided with crop pest arrival, alley‐cropping agroforestry is expected to benefit agricultural production through increased pest control potential. Alley‐cropping agroforestry may therefore be an important management strategy to develop more sustainable agricultural systems benefiting farmers and biodiversity alike. [ABSTRACT FROM AUTHOR]

Plant growth, crop yield, and pest and disease control are enhanced by PGPR (Plant growth promoting rhizobacteria), which are beneficial microorganisms found in a close symbiosis with plant roots. Phytohormones are secreted, nutrient uptake is improved, and soil properties along with the microbiological environment are regulated by these microorganisms, making them a significant focus in agricultural research. In this study, the efficient PGPR strain T1 was isolated and screened from tobacco inter-root soil, and identified and confirmed by ITS sequencing technology. Tobacco growth indicators and soil property changes were observed and recorded through potting experiments. The activities of key enzymes (e.g., sucrase, catalase, urease) in soil were further determined. High-throughput sequencing technology was utilized to sequence the soil microbial community, and combined with macro-genomics analysis, the effects of T1 strain on soil microbial diversity and metabolic pathways were explored. Following the application of T1, significant improvements were observed in the height, leaf length, and width of tobacco plants. Furthermore, the physical and chemical properties of the soil were notably enhanced, including a 26.26% increase in phosphorus availability. Additionally, the activities of key soil enzymes such as sucrase, catalase, and urease were significantly increased, indicating improved soil health and fertility. Comprehensive joint microbiomics and macrogenomics analyses revealed a substantial rise in the populations of beneficial soil microorganisms and an enhancement in metabolic pathways, including amino acid metabolism, synthesis, and production of secondary metabolites. These increase in beneficial microorganisms and the enhancement of their metabolic functions are crucial for plant growth and soil fertility. This study provides valuable references for the development of innovative microbial fertilizers and offers programs for the sustainable development of modern agriculture. [ABSTRACT FROM AUTHOR]

Carbofuran was once a highly toxic carbamate pesticide widely used in the prevention and control of pests in the agricultural field. In recent years, the problem of excessive residues of carbofuran in agricultural products has been reported, posing a great threat to human beings, mammals, and the environment. The maximum permitted levels of carbofuran in food have been established under different legislations worldwide. Therefore, it is necessary to strengthen supervision and rapid detection of carbofuran residues in food. Due to its high solubility and high yield, nanobody has been paid more attention in recent years. To further improve their sensitivity and stability to meet the demands of actual detection, the Nb5 was selected for in vitro evolution research. The structure models of Nb5 were constructed based on AlphaFold and verified by Ramachandran Plot, ERRAT, and Verify 3D. The key amino acid sites of Nb5/carbofuran were determined by LeadIT software and alanine scanning. The result showed that the carbofuran molecule was inserted into the pocket composed of CDR2, CDR3, and FR3. Nb5 and carbofuran were driven by hydrogen bonds and hydrophobic forces. Based on the confirmed mutation sites (Arg56, Trp58, Thr101, Val102, Ala103, Asp104, Cys105), a phage display saturation mutation library was established to screen mutants, and their performance was characterized. The IC50 of H10 was 17.6 ng/mL, the sensitivity was twice compared with that of the wild type Nb5, and the affinity constant was 1.54 x 106 L/mol. This study provides an effective idea for the affinity maturation of nanobodies against small molecule drugs in vitro. [ABSTRACT FROM AUTHOR]

In sustainable agricultural production, an efficient and environmentally-friendly nano-drug carrier system is a considerably efficient way to enhance the efficacy of pesticides and minimize ecological side effects. In this study, tetraethyl orthosilicate (TEOS) was used as the silicon source with ammonia as the catalyst for the synthesis of hollow mesoporous silica (HMS) by a self-template method. HMS was loaded with emamectin benzoate (EB) by co-solvent method to prepare nano-drug carrier system (HMS-EB). Chitosan (CS) was covalently modified on the surface of HMS-EB to obtain a good foliage adhesion nano-drug carrier system (CS@HMS-EB). Results showed that the prepared CS@HMS-EB had a regular spherical shape and uniform particle size, with an average particle size of about 200 nm and a drug loading rate of 45.3%. Through the covalent interaction, CS successfully adhered to the surface of HMS-EB, which significantly improved the wetting, adhesion and retention capacity of EB on the surface of maize leaves. Moreover, under UV-light irradiation, the degradation rate of EB loaded in the CS@HMS-EB decreased by 40% compared with EB alone. Meanwhile, CS@HMS-EB had a good sustained release property. Importantly, Both indoor and field bioactivity evaluation assays showed that compared with commercial 5% emamectin benzoate microemulsion (EB ME), CS@HMS-EB exhibited higher insecticidal activity against Spodoptera furgiperda larvae. This nano-drug carrier system does not contain organic solvents and has good insecticidal activity, which is expecting to play a broad application prospect in pest control. [ABSTRACT FROM AUTHOR]

Efficacy of two Indian isolates of entomopathogenic nematodes (EPNs) viz., Heterorhabditis bacteriophora MK256358 and Steinernema feltiae MK256355 were tested in laboratory against the larvae of cabbage butterfly, Pieris brassicae. Larval mortality was found directly proportional to initial inoculum level of infective juveniles (IJs). Susceptibility of larvae varied with respect to their variable size. H. bacteriophora MK256358 @ 25 IJs/larva caused 100% mortality to 3rd instar larvae at 72 h but @ 75 IJs/larva, the same mortality was achieved in 48 h. S. feltiae MK256355 @ 100 IJs/larva caused 100% mortality to 3rd instar larvae of P. brassicae at 48 h. H. bacteriophora MK256358 @ 25 and 100 IJs/larva resulted in 100% mortality to 4th and 5th instar larvae, respectively at 72 h, however S. feltiae MK256355 was unable to cause 100% mortality to either 4th or 5th instar larvae at any inoculum level or time period used in the study. LD50 and LT50 values of H. bacteriophora MK256358 were lower than S. feltiae MK256355 indicating that less nematode dose and time is required to kill 50% pest population. Reproduction capacity of nematode within the host was directly proportional to individual larval size and nematode inoculum level and for H. bacteriophora MK256358, it was higher and statistically significant (P ≤ 0.05) from S. feltiae MK256355. Our experimental findings open new avenues for utilization of EPNs against P. brassicae and set the basis for safe insect pest management programme. [ABSTRACT FROM AUTHOR]

Biological control has long been recognized for its economic and ecological benefits as a pest management approach, but the use of non-native natural enemies has raised concerns about potential risks and unintended consequences. Indigenous predatory ladybeetles, such as Oenopia conglobata, play a crucial role in controlling many sap-sucking pests, including Agonoscena pistaciae, which is the most destructive pest of pistachio trees in Iran and other pistachio-growing regions worldwide. However, the abundance of Oenopia conglobata has recently decreased due to the presence of an adventive predatory ladybeetle, Menochilus sexmaculatus. To better understand the potential risks associated with this adventive species, we investigated the functional responses of female adults of M. sexmaculatus and O. conglobata against the third and fourth instar nymphs of A. pistaciae and Aphis gossypii. Our findings revealed that both predators exhibited a type II functional response, with O. conglobata demonstrating a significantly higher attack rate against A. pistaciae nymphs than A. gossypii nymphs. In contrast, prey species did not have a significant effect on the attack rate of M. sexmaculatus. Notably, M. sexmaculatus displayed the highest predation rate and voracity against both prey species. These results provide valuable insights into the potential risks of M. sexmaculatus for indigenous predatory species like O. conglobata. The fact that M. sexmaculatus exhibits higher voracity for both prey species than O. conglobata does suggest that it may pose a threat to the native ladybeetle population, highlighting the need for further research and careful consideration when introducing non-native natural enemies for pest management. [ABSTRACT FROM AUTHOR]

Effective pest population monitoring is crucial in precision agriculture, which integrates various technologies and data analysis techniques for enhanced decision-making. This study introduces a novel approach for monitoring lures in traps targeting the Mediterranean fruit fly, utilizing air quality sensors to detect total volatile organic compounds (TVOC) and equivalent carbon dioxide (eCO2). Our results indicate that air quality sensors, specifically the SGP30 and ENS160 models, can reliably detect the presence of lures, reducing the need for frequent physical trap inspections and associated maintenance costs. The ENS160 sensor demonstrated superior performance, with stable detection capabilities at a predefined distance from the lure, suggesting its potential for integration into smart trap designs. This is the first study to apply TVOC and eCO2 sensors in this context, paving the way for more efficient and cost-effective pest monitoring solutions in smart agriculture environments. [ABSTRACT FROM AUTHOR]

The house fly, Musca domestica, is a cosmopolitan species known for its pestiferous nature and potential to mechanically vector numerous human and animal pathogens. Control of adult house flies often relies on insecticides formulated into food baits. However, due to the overuse of these baits, insecticide resistance has developed to all insecticide classes currently registered for use in the United States. Field populations of house flies have developed resistance to imidacloprid, the most widely used neonicotinoid insecticide for fly control, through both physiological and behavioral resistance mechanisms. In the current study, we conducted a comprehensive analysis of the inheritance and dominance of behavioral resistance to imidacloprid in a lab‐selected behaviorally resistant house fly strain. Additionally, we conducted feeding preference assays to assess the feeding responses of genetic cross progeny to imidacloprid. Our results confirmed that behavioral resistance to imidacloprid is inherited as a polygenic trait, though it is inherited differently between male and female flies. We also demonstrated that feeding preference assays can be instrumental in future genetic inheritance studies as they provide direct insight into the behavior of different strains under controlled conditions that reveal, interactions between the organism and the insecticide. The findings of this study carry significant implications for pest management and underscore the need for integrated pest control approaches that consider genetic and ecological factors contributing to resistance. [ABSTRACT FROM AUTHOR]

Understanding the genetic basis of adaptive evolution following habitat expansion can have important implications for pest management. The pink rice borer (PRB), Sesamia inferens (Walker), is a destructive pest of rice that was historically restricted to regions south of 34° N latitude in China. However, with changes in global climate and farming practices, the distribution of this moth has progressively expanded, encompassing most regions in North China. Here, 3 highly differentiated subpopulations were discovered using high‐quality single‐nucleotide polymorphism and structural variant datasets across China, corresponding to northern, southern China regions, and the Yunnan‐Guizhou Plateau, with significant patterns of isolation by geographic and environmental distances. Our estimates of evolutionary history indicate asymmetric migration with varying population sizes across the 3 subpopulations. Selective sweep analyses estimated strong selection at insect cuticle glycine‐rich cuticular protein genes which are associated with enhanced desiccation adaptability in the northern group, and at the histone‐lysine‐N‐methyltransferase gene associated with range expansion and local adaptation in the Shandong population. Our findings have significant implications for the development of effective strategies to control this pest. [ABSTRACT FROM AUTHOR]

Botanical insecticides are the origin of all insecticidal compounds. They have been widely used to control pests in crops for a long time. Currently, the commercial production of botanical insecticides extracted from plants is limited because of insufficient raw material supply. Synthetic biology is a promising and effective approach for addressing the current problems of the production of botanical insecticides. It is an emerging biological research hotspot in the field of botanical insecticides. However, the biosynthetic pathways of many botanical insecticides are not completely elucidated. On the other hand, the cytotoxicity of botanical pesticides and low efficiency of these biosynthetic enzymes in new hosts make it still challenging for their heterologous production. In the present review, we summarized the recent developments in the heterologous production of botanical insecticides, analyzed the current challenges, and discussed the feasible production strategies, focusing on elucidating biosynthetic pathways, enzyme engineering, host engineering, and cytotoxicity engineering. Looking to the future, synthetic biology promises to further advance heterologous production of more botanical pesticides. [ABSTRACT FROM AUTHOR]

Background: Remote sensing based on multispectral imaging may be useful for detecting vegetation stress responses in agriculture. Objectives: To evaluate the potential of orbital multispectral imaging in discriminating the most effective strategies for reducing plant-parasitic nematode populations, thereby preventing yield losses in coffee production. Methods: Coffee plants were treated with eleven treatments, including Bacillus spp. isolates, commercial biological products, commercial chemical nematicides, and water (control group). Initial and final nematode populations in the soil were quantified, and surface reflectance data were collected using the Planet orbital multispectral sensor. The data were classified using the random tree algorithm. Results: The population of plant-parasitic nematodes was reduced by 35.90% and 55.13% following the application of B. amyloliquefaciens isolate B266 and B. subtilis isolate B33, respectively. Under the conditions of this experiment, multispectral imaging accurately discriminated the most nematicidal treatments, with a global accuracy of 80%. Conclusions: Orbital multispectral imaging can discriminate the most effective treatments used for nematode management in coffee plants, highlighting its potential as a supportive tool in agriculture. [ABSTRACT FROM AUTHOR]

Bujangseta is a technology used throughout the year to produce citrus fruits. This technology has economically succeeded in increasing the income of citrus farmers by 23.5%. Sustainable management of bujangseta technology is neede, one of which is maintaining the sustainability of the agroecosystem. A stable agroecosystem ensures the availability of ecosystem services through nutrient cycling and the natural control of pest and disease populations. The stability of agroecosystems is coherent with the high biodiversity of arthropods. This study aims to evaluate the effect of applying the citrus plant bujangseta in Karangwidoro village and Krajan village, Dau District, Malang Regency, East Java, from June to December 2022. The two application methods were light trapping (lighttrap) and pit trap (pitfall glass), and the results were analyzed using a t-test. The diversity index, species richness index, species evenness index, and dominance index were calculated. The results showed that the detrivores of the Collembola group dominated the diversity of arthropods, the primary pest of the Diaphorina citri, and the predatory arthropods of Paederus sp. and Braconidae. Diversity and community structure of arthopods indicated there at significant differences in diversity, dominance, evenness, and species richness in those two locations. Bujangseta in the Krajan has a higher community structure compared to the Karangwidoro, showing that the ecosystem at the Krajan location is better preserved. [ABSTRACT FROM AUTHOR]

Introduction: Cotton production in Tanzania is facing significant challenges due to insect pests that cause extensive damages to the crop. The most notable pests include the African bollworm (Heliothis armigera Hubner), Spiny bollworm (Earias biplaga Walker), Cotton stainers (Dysdercus sidae (Herrich-Schaeffer), Cotton Aphids (Aphis gossypii Glover), Thrips (Thrips tabaci Lindeman), Jassids (Amrasca biguttula, Bigutula), Leafhoppers (Cicadellidae jassidae), andWhiteflies (Bemisia tabaci Genn). If left uncontrolled, these pests can cause up to 60% damage to the crop. Despite the importance of cotton and the fact that most of these pests are endemic, there are scanty knowledge on the dynamics and distribution of cotton pests across the seasons of the year and crop's phenological growth stages (germination, vegetative growth, flowering and boll formation) in areas under repeated cultivation of the crop in Tanzania. Here we report on the influence of seasons and cotton's phenological stages on the abundance, diversity, distribution and richness of cotton insect pests. Methods: The studywas conducted in the Misungwi district for two cotton-growing seasons, using the UKM08 cotton variety. Stick traps and handpickingmethods were deployed in catching the cotton insect pests. Results: On average, a total of 8,500 insect specimen of diverse families and species were collected every season. The four dominant species among the collected were Aphis gossypii (17.37%), Amrasca biguttula (11.42%), Nezara viridura (10.7%), and Bemisia tabacci (10.68%). Both cotton phenological growth stages and seasons significantly (p<0.05) influenced the abundance, diversity, distribution and richness of cotton insect pests. In particular, the phenological growth stage 3 exhibited greater diversity of insect pests. The pests' distribution patterns remained relatively uniform across the crop growth stages. Discussion: Findings from the present study could contribute to developing sustainable pest management strategies in areas under repeated cotton production in Tanzania and elsewhere. [ABSTRACT FROM AUTHOR]

Rice (Oryza sativa L.) is a vital crop with enormous economic and nutritional importance in Nepal and worldwide. The yellow stem borer (YSB), Scirpophaga incertulas (Walker) (Lepidoptera: Crambidae), is a monophagous severe pest that poses a substantial threat to rice crops, causing significant yield losses by its larva feeding on stems and tillers. This review covers the life cycle, distribution, damage, and management and control strategies of YSB. YSB primarily targets rice plants, causing symptoms like dead hearts and whiteheads. Integrated pest management (IPM) strategies are crucial for effective YSB control. Cultural practices include selecting resistant rice varieties, using Bacillus thuringiensis (Bt) incorporated transgenic rice plants, synchronizing planting to reduce generations, and promoting natural enemies by conserving their habitats. Mechanical methods involve pheromone and light traps to disrupt mating and reduce adult populations. Biological control methods utilize natural predators like beetles and spiders and parasitoids such as Cotesia flavipes and Trichogramma spp. In contrast, chemical control involves targeted insecticide application when pest populations reach economic threshold levels that is when 5–10% dead hearts are seen. The article emphasizes the importance of IPM techniques in reducing YSB infestation and fostering sustainable rice production. These approaches not only help to lower the YSB population but also contribute to environmental preservation and long-term agricultural viability. As rice is a staple food for millions, effective management of YSB becomes crucial for guaranteeing food security and livelihoods across the globe. [ABSTRACT FROM AUTHOR]

Beneficial microbes induce resistance in plants (MIR), imposing both lethal and sublethal effects on herbivorous insects. We argue that herbivores surviving MIR carry metabolic and immunological imprints of MIR with cascading effects across food webs. We propose that incorporating such cascading effects will strongly enhance the current MIR research framework. [ABSTRACT FROM AUTHOR]

The genus Phytophthora contains more than 100 plant pathogenic species that parasitize a wide range of plants, including economically important fruits, vegetables, cereals, and forest trees, causing significant losses. Global agriculture is seriously threatened by fungicide resistance in Phytophthora species, which makes it imperative to fully comprehend the mechanisms, frequency, and non-chemical management techniques related to resistance mutations. The mechanisms behind fungicide resistance, such as target-site mutations, efflux pump overexpression, overexpression of target genes and metabolic detoxification routes for fungicides routinely used against Phytophthora species, are thoroughly examined in this review. Additionally, it assesses the frequency of resistance mutations in various Phytophthora species and geographical areas, emphasizing the rise of strains that are resistant to multiple drugs. The effectiveness of non-chemical management techniques, including biological control, host resistance, integrated pest management plans, and cultural practices, in reducing fungicide resistance is also thoroughly evaluated. The study provides important insights for future research and the development of sustainable disease management strategies to counter fungicide resistance in Phytophthora species by synthesizing current information and identifying knowledge gaps. [ABSTRACT FROM AUTHOR]

The use of pathogens and other natural enemies to suppress pest populations is a key component of integrated pest management (IPM) strategies. As biocontrol agents may interact synergistically, additively, or antagonistically, it is crucial to assess their interactions for effective pest management. In this study, we investigated the effect of three entomopathogenic fungus (EPF) species—Beauveria bassiana (Bals.‐Criv.) Vuill. CEP 091, Akanthomyces muscarius (Petch) Spatafora, Kepler & B. Shrestha CEP 182, and Cordyceps fumosorosea (Wize) Kepler, B. Shrestha & Spatafora CEP 315 (all Hypocreales)—on the survival, fecundity, and searching behavior of Orius insidiosus (Say) (Hemiptera: Anthocoridae). All three EPF species were found to be pathogenic to O. insidiosus, resulting in a significant decrease in survival compared with the control group. Fecundity remained unaffected by the fungal treatments. No effect of EPF on searching behavior of the predator was found. In relation to the searching behavior, individuals of 2 days post‐infections remained in the food patch longer than individuals of 7 days post‐infections, regardless of treatment. These findings provide valuable insights into the compatibility of EPF and O. insidiosus as combined biocontrol agents for managing whiteflies, aphids, and thrips. Understanding the interactions between EPF and predatory insects is crucial for developing IPM strategies that maximize pest control efficacy. Further studies should explore the long‐term effects of EPF on O. insidiosus populations and their overall impact on pest management in agricultural systems. [ABSTRACT FROM AUTHOR]

In the scientific toxicity evaluations of insecticides against larvae of Tuta absoluta (Tomato pinworm), a leaf coverage rate of 100 % or very close to this value is sought to guarantee the contact of the insect with the chemical product. However, deposition can change according to the different pesticide application methods used in field productions; consequently, interfering with pest control. From this perspective, this study evaluated the mortality of Tuta absoluta and the deposited volume dispersion on tomato leaflets as a function of droplet sizes and the method of chemical treatment with abamectin. For that purpose, an experiment was conducted in a randomized block design with five treatments and five replications. The treatments consisted of different chemical treatment methods: immersion, hydraulic spraying with three droplet sizes, and pneumatic spraying, in which the following parameters were evaluated: larval mortality, volume retained (deposition), surface density of active ingredient, droplet density, and coverage percentage. The different chemical treatments with abamectin provided larval mortality rates above 90%. Furthermore, the highest mortality values (98% and 95.9%) were associated; respectively, with the sprayings that provided the highest droplet densities: hydraulic spraying with average droplets (181 droplets cm-2) and pneumatic spraying with very fine droplets (256 droplets cm-2), the latter using an application volume 84% lower (80 L ha-1) than the lowest volume recommended by the manufacturer (500 L ha-1). [ABSTRACT FROM AUTHOR]

Exploring the impact of low-temperature storage on the fitness of natural enemy insects is crucial for practical field applications because this parameter directly influences their potential for population growth and effective pest control. Eocanthecona furcellata (Wolff) (Hemiptera: Pentatomidae) is widely used in biological pest control. This study aimed to identify optimal storage stages, temperatures, and durations for E. furcellata to produce high-quality individuals for practical use. The quality of E. furcellata after storage was evaluated by assessing parameters such as predatory capacity and fecundity, along with age-stage, two-sex life table. The findings revealed that the adult stage was the optimal storage form for E. furcellata , and the most favorable temperature for storage was 12 °C. Adult females had the highest predatory ability after 15 days of storage at 12 °C. Although survival rates declined with prolonged storage, they remained above 50% after 30 days, and longevity, fecundity, and predatory capacity of surviving individuals remained comparable to those of individuals in the control group (rearing at a constant temperature of 26 °C without low-temperature storage). The effects of low-temperature storage extended to the F1 generation of E. furcellata , which exhibited maximum mean longevity, fecundity, net reproductive rate, and mean generation time as well as fastest population growth after 30 days of storage at 12 °C. These results can be used to achieve optimal low-temperature storage conditions for E. furcellata production, particularly for extending its shelf life. [ABSTRACT FROM AUTHOR]

The cotton bollworm, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae), is a significant cotton pest worldwide. Bacillus thuringiensis (Bt) cotton producing Cry1Ac has been used since 1997 for the control of this pest in China and a significant increase in H. armigera resistance to Cry1Ac has occurred in northern China. To mitigate resistance evolution, it is necessary to develop and plant pyramided 2- and 3-toxin Bt cotton to replace Cry1Ac cotton. For sustainable use of pyramided Bt cotton, we used diet overlay bioassays to measure the baseline susceptibility of H. armigera to Cry2Ab in 33 populations collected in 2017, 2018, and 2021 in 12 locations from major cotton-producing areas of China. The lethal concentration killing 50% (LC50) or 99% (LC99) of individuals from the populations ranged from 0.030 to 0.138 µg/cm2 and 0.365 to 2.964 µg/cm2, respectively. The ratio of the LC50 for the most resistant and susceptible population was 4.6, indicating moderate among-population variability in resistance. The susceptibility of H. armigera to Cry2Ab did not vary significantly over years. A diagnostic concentration of 2 µg/cm2 was calculated as twice the LC99 from an analysis of pooled data for the field-collected populations. This concentration discriminated well between susceptible and resistant individuals, as it killed all larvae from a susceptible laboratory strain and 0%, 0%, and 23% of larvae from 3 laboratory strains with > 100-fold resistance to Cry2Ab. These baseline susceptibility data and diagnostic concentration for Cry2Ab will be useful for monitoring the evolution of H. armigera resistance to pyramided Bt cotton in China. [ABSTRACT FROM AUTHOR]

The cryptic behavior that characterizes bed bugs (Cimex lectularius L.) makes them one of the most difficult indoor pests to control. Thus, the use of products with long residual efficacy is a key component of successful bed bug management. Aprehend is a biopesticide for bed bug control, whose active ingredient is the entomopathogenic fungus, Beauveria bassiana. This product shows promise for extended bed bug control against pyrethroid-resistant bed bugs, causing mortality days after contact with the treated surface. However, it is unclear how surface type and distance traveled by bed bugs over a treated surface impact efficacy. Therefore, we evaluated the efficacy of different Aprehend band widths applied to different substrates commonly encountered by bed bugs, looking specifically at average time to mortality and overall mortality. Our findings indicate that exposure to fresh applications of Aprehend resulted in high mortality when bed bugs traveled very short distances (1 cm) over some Aprehend-treated surfaces (vinyl tile and cotton jersey fabric), while exposure to other treated surfaces (unfinished pinewood and painted drywall) resulted in comparatively lower mortality even over longer distances (5 cm). Furthermore, we found that bed bugs crawling on cotton jersey fabric picked up a significantly higher number of spores compared to unfinished pinewood. These results indicate that applications of Aprehend as 5 cm (2 inches) bands according to label recommendations can be effective, although surface type is an important determinator of efficacy. This information will help guide pest management professionals in their use of B. bassiana for bed bug control. [ABSTRACT FROM AUTHOR]

The fall armyworm, Spodoptera frugiperda (J. E. Smith, 1797) (Lepidoptera: Noctuidae), is a significant global pest, that exhibits 2 discernible strains, corn strain (CS) and rice strain (RS). After initial detection in the eastern hemisphere in 2016, the dominant strain was identified as RS based only on cytochrome C oxidase subunit I (COI) mitochondrial gene from limited samples from various countries, including Thailand. This study aimed to assess strain and haplotype variation in the S. frugiperda populations in Thailand using both mitochondrial COI and nuclear triosephosphate isomerase (Tpi) genes. Analyses of COI sequences (n  = 105) revealed 2 predominant haplotypes, COI CSh4 (82.86%) and COI RSh1 (17.14%), and the analyses of Tpi sequences (n  = 99) revealed 6 haplotypes, with Tpi Ca1a (53.53%) being the most prevalent. Of the 98 caterpillar samples, the majority exhibited true CS (83.67%) for both genes. Meanwhile, interstrain hybrids, indicated by gene discordance, accounted for the minority (16.33%). Interestingly, despite the initial dominance of RS during the 2018 outbreak, the current study identified CS as the prevalent strain across all localities in Thailand. These findings suggested a shift in S. frugiperda dynamics in Thailand that was possibly influenced by factors, such as competitive exclusion principle, pesticide usage in rice cultivation, and preferences for corn over rice. Our study suggests a need to reexamine the previous reports of rice-strain dominance in various countries in the eastern hemisphere after the initial invasion. [ABSTRACT FROM AUTHOR]

Wolbachia , a prevalent intracellular symbiotic bacterium in insects, plays a significant role in insect biology. Ectropis grisescens (Warren; Lepidoptera: Geometridae) is a devastating chewing pest distributed in tea plantations throughout China. However, it is unclear how Wolbachia titers affect the fitness and reproduction of E. grisescens. In this study, the impacts of 3 different infection lines, naturally Wolbachia- infected, Wolbachia- uninfected, and Wolbachia transinfected, regarding the life history traits of E. grisescens, were evaluated using the age-stage, 2-sex life table. Wolbachia infection shortened preadult duration and preoviposition periods and increased the fecundity, net reproductive rate, and finite rate of increase. Meanwhile, population projection indicated that E. grisescens population size with Wolbachia infection can increase faster than without. These results indicate that Wolbachia plays a regulatory role in the fitness of E. grisescens. It is also noted that the life history parameters of E. grisescens may positively correlate with Wolbachia titers. These findings could aid in pest management in tea gardens. [ABSTRACT FROM AUTHOR]

Plant protection products derived from plant material are proposed to be a sustainable alternative to conventional synthetic chemical pesticides. This study determines the efficacy of a commercially available bioinsecticide based on garlic (Allium sativum L.; Asparagales: Amaryllidaceae) extract against vine weevil (Otiorhynchus sulcatus F.; Coleoptera: Curculionidae) eggs and larvae in contact, fumigation and a combination of contact and fumigation bioassays under laboratory conditions. Results showed that garlic significantly reduced egg hatch rate compared to the control group when applied as a fumigant. Similarly, the egg hatch rate was reduced compared to the control group when garlic was applied as combined contact and fumigant applications. No effect was observed when the garlic product was applied as a contact application. The bioinsecticide significantly reduced larval survival when either contact or fumigant applications were used. A combined contact and fumigant effect was shown also when vine weevil eggs were exposed to the bioinsecticide for 30 days in plastic containers containing growing media. The number of larvae recovered after this period was significantly reduced compared to the control group. This study demonstrates the potential of garlic-based bioinsecticides, such as Pitcher GR, for vine weevil control. Further studies are, however, needed to determine the efficacy of such bioinsecticides under field conditions and investigate how these products can be most effectively used as a part of a wider vine weevil integrated pest management program. [ABSTRACT FROM AUTHOR]

The soybean pod borer Leguminivora glycinivorella (Matsumura) is one of the most important soybean pests and often causes serious damage to Glycine max (L.) Merr. a leading source of dietary protein and oil in animal feed. However, the potential distribution patterns of this economically important pest and its driving factors require further investigation. Here, we used the optimized MaxEnt model to predict the potential distribution of this pest with multiple variables associated with climate, land use, and host plant, at its recorded range and a globe scale. Based on 4 variable combinations, the results show that the current suitable habitats of L. glycinivorella are primarily distributed in most of China, the Korean Peninsula, and Japan. Whereas no suitable area is present in other continents. In future projections, the suitable region shows a slight northward expansion compared with the result predicted with current climatic conditions, and the suitable areas of almost all future projections were stable in size. Among the 9 bioclimatic factors, BIO03 (isothermality) consistently highly contributes to the predictions, indicating that temperature may be a key factor influencing the habitat distribution of L. glycinivorella. Comparative analyses of projections further show that non-climatic factors are informative in the modeling as routinely used bioclimate variables. The spatio-temporal distribution patterns of suitable habitats and the regulatory factors predicted in this study could provide important guidance for L. glycinivorella management. [ABSTRACT FROM AUTHOR]

The German cockroach, Blattella germanica (L.) (Blattodea: Ectobiidae), is a ubiquitous pest in affordable housing. They represent a major threat to human health due to their contribution of asthma-exacerbating allergens and the potential to transfer pathogenic microorganisms indoors. Despite well-documented pyrethroid resistance, pyrethroid-based broadcast residual insecticide products are often used by residents to control cockroaches in their homes. Additionally, there is little empirical independent testing of these products. Thus, it remains unclear how effective these commonly used do-it-yourself products are at controlling German cockroaches. This study represents a comprehensive examination of the efficacy of these products with direct, limited, and continuous exposure assays on a variety of common household surfaces on field populations of cockroaches with varying levels of pyrethroid resistance. While most products performed well when applied directly to test insects, mortality was substantially lower across all surfaces with limited exposure (30 min). In continuous exposure assays on a nonporous surface, products took at least 24 hr to cause 100% mortality in a field population, with some products taking up to 5 d to achieve 100% mortality. The findings of this study demonstrate a lack of residual efficacy from common pyrethroid-based consumer-use pesticides products. Given that it is not feasible to find and treat every cockroach in a home directly, the residuality of spray-based formulations is critical for products designed to control German cockroaches. Without residual efficacy, as shown in the consumer aerosol and spray products tested, we expect these products to add little to no value to cockroach control. [ABSTRACT FROM AUTHOR]