1. Alichondrichlorin, a Novel Chlorohydrin-Containing Natural Product With Tumoral Cytotoxic Activity Isolated From the Planctomycetota Bacterium Alienimonas chondri LzC2 T .
- Author
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Vitorino IR, Santos JDN, Crespo G, Pérez-Victoria I, Martín J, Rodriguez L, Ramos MC, Martins TP, Leão PN, Vicente F, Vasconcelos V, Lage OM, and Reyes F
- Subjects
- Humans, Cell Line, Tumor, Biological Products pharmacology, Biological Products chemistry, Biological Products isolation & purification, Mass Spectrometry, Magnetic Resonance Spectroscopy, Cell Proliferation drug effects, Cell Line, Cell Survival drug effects, Antineoplastic Agents pharmacology, Antineoplastic Agents chemistry
- Abstract
To address the on-going need for chemical novelty and the limited information on Planctomycetota secondary metabolism, we focused on exploring the recently isolated marine planctomycetal strain Alienimonas chondri LzC2
T to uncover its potential production of novel compounds. This work contemplates the description of a large-scale cultivation study of strain LzC2T , followed by metabolite extraction and compound isolation using chromatographic approaches, which resulted in the isolation of a novel molecule designated as alichondrichlorin. Structural elucidation of this new molecule was accomplished by a combination of high-resolution mass spectrometry and nuclear magnetic resonance. The molecule was additionally screened for anti-proliferative bioactivity against human tumoral and non-tumoral cell lines. These cytotoxicity assays revealed a targeted effect of alichondrichlorin in the growth of tumoral cell lines, especially human breast adenocarcinoma MCF-7 cell line (EC50 = 4.06 μM) without effect on the human non-tumoral THLE-2 cell line (EC50 > 50 μM)., (© 2025 The Author(s). Microbial Biotechnology published by John Wiley & Sons Ltd.)- Published
- 2025
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