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In this work we examined the contents of 14 polyphenols in white lupin (Lupinus albus L.) samples cultivated in Southern Italy by the optimisation and validation of a LC-HRMS method. The validation of the LC-HRMS method showed linearity results r2 > 0.989 and recovery values between 71 and 119% for a very wide range of concentrations. Ellagic acid was the most abundant polyphenol, with mean concentrations of 16271.86 ± 19798.53 μg/Kg, followed by apigenin (2749.51 ± 889.95 μg/Kg). A significant variability in ellagic acid contents was found between the areas of cultivation examined (p < 0.05). As far as we know, this work provides the first data on the polyphenols contents of white lupins cultivated in Italy. The comparison with other study confirms the role of the cultivation area for the determination of the polyphenol's contents. The study also confirms white lupins as a promising source of antioxidant and anti-inflammatory substances in a balanced diet. [ABSTRACT FROM AUTHOR]

The presence of polyphenols was examined in carob pods ( Ceratonia siliqua ) from Southern Italy 90 days after harvest by the validation of a reliable LC-HRMS method. A greater abundance of Apigenin (51490.22 ± 34399.16 μg/Kg) and Myricetin (24897.92 ± 108332.05 μg/Kg) compared to previous research works conducted in Mediterranean countries. Significant differences in the polyphenol content between sampling areas ( p  < 0.05) were observed, particularly differences in hesperidin and myricetin. These differences confirmed the role of geochemical and climatic conditions in the variation of polyphenol content. This study is a first regarding the phenolic content of carob pods from Southern Italy, confirming the presence of these substances even long after harvest and that carob pods are valuable sources of phenolic substances that may be useful in the prevention of diseases related to oxidative stress.