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Spatial resources have become the basis for farmers to survive and develop, and to participate in the distribution of industrial benefits. In the process of capitalization of rural tourism space, redefining the connotation and scope of rural livelihood space is the capital basis for farmers to obtain power and share value. Based on the tourism industry-embedded rural livelihood space and the research paradigm of spatial "gene map", this paper analyzes and integrates the rural livelihood space, and establishes the gene system of the rural livelihood space in three dimensions: geographical space, economic value space and social cultural space. Taking Matao Village in Shandong Province as an empirical research object, the gene system of livelihood space is visualized and practiced, and the gene bank of the rural livelihood space is established, and various gene elements are projected on the rural geographical space, and the spatial distribution characteristics of the three types of livelihood space gene elements are summarized. Here, it is proposed that the rural livelihood space of Matao Village presents spatial mismatch problems such as: the relative independence of economic value space and social cultural space, the intensified effect of differential land rent at village-level, the lack of social attributes of cultural space, and the relative weakening of residents spatial power. Aiming at the above problems, strategic suggestions for optimizing village livelihood space driven by tourism industry are put forward. [ABSTRACT FROM AUTHOR]