Microplastics (MPs) are distributed throughout the world oceans and represent one of the greatest environmental concerns of marine pollution. In the Gulf of Cadiz (GoC), MPs are found throughout the water column, on the seafloor, and accumulated within commercial marine species, primarily due to discharges from the main estuaries. The aim of this study was to analyse the transport pathways, spatial distribution, and accumulation regions of MPs in the GoC based on their density and source. For this, a Lagrangian transport model was coupled to a high-resolution hydrodynamic model and four particle sources were considered: Cape San Vicente, Guadiana Estuary, Guadalquivir Estuary, and Bay of Cadiz/Guadalete River. To account for the diversity of plastics detected in the GoC, particles with ten different densities were used, from low-density to high-density polymers. This study indicates that a significant proportion of low-density MPs accumulate near their sources and within the top few centimetres of the water column due to local surface currents. The Guadalquivir and Guadiana estuaries are the primary contributors to the high accumulation of low-density MPs on the GoC eastern shelf, consistent with previous field studies identifying these estuaries as the main sources of MPs into the region, including polyethylene and polypropylene. In contrast, the Bay of Cadiz/Guadalete River seems to be the primary source of low-density MPs in offshore waters within the uppermost meter of the water column, influenced by local mesoscale features. The Guadalquivir Estuary seems to be the main source of high-density MPs into the continental shelves, such as polystyrene, polyamide, and polyvinyl chloride, followed by the Bay of Cadiz/Guadalete River, and to a lesser extent, the Guadiana estuary. These MPs accumulate near their sources at depths of 3.5 to 50 m due to their high sinking rates, but can also be transported offshore by deep currents, either northwards along the Portuguese offshore waters or westwards off the GoC offshore region., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)