74 results on '"Shellfish analysis"'
Search Results
2. Evaluation of the texture characteristics and taste of shrimp surimi with partial replacement of NaCl by non‑sodium metal salts.
- Author
-
Qian X, Lin S, Chen T, Li S, Wang S, Li C, Wang R, and Sun N
- Subjects
- Animals, Humans, Shellfish analysis, Osmolar Concentration, Food Handling, Taste, Sodium Chloride chemistry, Sodium Chloride analysis, Penaeidae chemistry, Calcium Chloride chemistry, Potassium Chloride chemistry
- Abstract
Ionic strength plays a significant role in the aggregation behavior of myofibrillar proteins. The study investigated the effects of KCl or CaCl
2 as substitutes for NaCl on the gel properties and taste of shrimp surimi at a constant ionic strength (IS = 0.51). Increased KCl substitution ratio resulted in a reduction in α-helix content and an increase in β-sheet content of myofibrillar proteins, thereby enhancing water holding capacity. Optimal KCl substitutions (1.5% NaCl +1.94% KCl) contributed to maintaining the desired taste and improving gel properties. CaCl2 facilitates the extraction and dissolution of myofibrillar proteins, resulting in an organized and dense gel network with significant water-holding capacity. However, excessive additions (>1.27%) resulted in a notable decrease in taste and gel strength due to excessive aggregation and precipitation of myofibrillar proteins. These findings provide a solid theoretical foundation for production of high-quality, low-salt shrimp surimi., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this study., (Copyright © 2024 Elsevier Ltd. All rights reserved.)- Published
- 2024
- Full Text
- View/download PDF
3. Determination of Nanoparticles and Elements in Blue Mussels ( Mytilus edulis ) along the Norwegian Coastline.
- Author
-
Bruvold AS, Valdersnes S, Bienfait AM, Sanden M, and Loeschner K
- Subjects
- Animals, Norway, Environmental Monitoring, Shellfish analysis, Mytilus edulis chemistry, Mytilus edulis metabolism, Nanoparticles chemistry, Water Pollutants, Chemical analysis, Water Pollutants, Chemical chemistry
- Abstract
Our work aimed to examine nanoparticle levels in 69 distinct pooled mussel samples along the Norwegian coastline, considering samples from different environmental contexts, including natural locations, potentially polluted hotspots, and mussel farms. Single-particle ICP-MS was utilized to determine particle mass and number concentrations at environmentally relevant levels in addition to the total content of 11 elements: aluminum, barium, cerium, copper, iron, manganese, lead, silicon, silver, titanium, and zirconium. Results showed nanoparticle mass concentrations of few ng/g up to tens of μg/g and number concentrations of 10
6 to 109 particles/g (wet weight). Certain urban and industrially impacted locations were linked to increased levels of, e.g., silver, lead, cerium, zirconium, and titanium NPs. Farmed mussels exhibited lower concentrations. However, natural variations were considerable, and impacted locations mostly did not differ from the highest levels in pristine areas. The study presents the first extensive survey of NPs of 11 different elements in marine biota and provides evidence of increased levels of NPs in areas with anthropogenic activities.- Published
- 2024
- Full Text
- View/download PDF
4. RuSiNPs@N,S-GQDs as self-enhanced anodic electrochemiluminescent immunobeacons for the highly sensitive quantitation of okadaic acid in shellfish.
- Author
-
Li S, Peng J, Lin X, Chen J, Wu Y, Chen Q, and Chen X
- Subjects
- Animals, Electrodes, Immunoassay methods, Biosensing Techniques methods, Silicon Dioxide chemistry, Food Contamination analysis, Nitrogen chemistry, Sulfur chemistry, Nanoparticles chemistry, Okadaic Acid analysis, Okadaic Acid immunology, Graphite chemistry, Quantum Dots chemistry, Shellfish analysis, Luminescent Measurements methods, Electrochemical Techniques methods, Limit of Detection
- Abstract
A competitive electrochemiluminescence (ECL) immunosensor is proposed to accurately and rapidly assess okadaic acid (OA) levels in shellfish using a novel self-reinforced solid-state ECL marker, which is essential for ensuring seafood safety. Graphene quantum dots doped with nitrogen and sulfur (N,S-GQDs) were synthesized, for the first time, through the electrolysis of graphite in 3-(N-morpholine) propane sulfonic acid solution. Intriguingly, these N,S-GQDs exhibited exceptional co-reactant properties, significantly enhancing the anodic ECL performance of Ru(bpy)
3 2+ in a phosphate-buffered saline solution. Following the functionalization of Ru(bpy)3 2+ -doped silica nanoparticles (RuSiNPs) with poly(diallyldimethylammonium) chloride, we achieved a well-dispersed assembly of N,S-GQDs on the exterior of the RuSiNPs through electrostatic interactions. Importantly, the core-shell structure of RuSiNPs@N,S-GQDs efficiently encapsulated both the luminophore and co-reactant, thus improving the transfer rates of electrons, shorting interaction distances, and reducing energy loss during light emission. Leveraging this "bright" ECL beacon, the ECL immunosensor demonstrated remarkable analytical performance, yielding a low half maximal inhibitory concentration (IC50 ) of 0.14 ng mL-1 , an extensive linear range spanning 0.003-40 ng mL-1 , and impressively low limit of detection of 0.001 ng mL-1 for OA determination., Competing Interests: Declarations Ethical approval No human or animal samples were involved in this study. Conflict of interest The authors declare no competing interests., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)- Published
- 2024
- Full Text
- View/download PDF
5. Assessment of potentially toxic element contamination in commercially harvested invertebrates from the Beibu Gulf, China.
- Author
-
Zhao S, Su Q, Huang L, Wang C, Ma J, Zhu L, Cheng Y, Yang X, Yang Y, and Kang B
- Subjects
- Animals, China, Risk Assessment, Metals, Heavy analysis, Food Chain, Shellfish analysis, Water Pollutants, Chemical analysis, Environmental Monitoring, Invertebrates
- Abstract
Marine pollutants, especially potentially toxic elements (PTEs), increasingly threaten the ecological environment and fishery resources of the Beibu Gulf due to their bioaccumulative nature, toxicity, and persistence. However, the occurrences of multiple PTEs in marine invertebrates within this region remains unclear. Hence, a total of 18 species of commercially harvested invertebrates (shrimp, crab, cephalopod, shellfish, and sea cucumber) were collected from the Beibu Gulf, and the concentrations of nine important PTEs (As, Cd, Cr, Cu, Hg, Mn, Ni, Pb, and Zn) were examined. Subsequent stable isotope analysis for δ
13 C and δ15 N facilitated investigations into biomagnification and human health risk assessment. The results showed that, except for As, the concentrations of the PTEs in the invertebrates were below the national safety limits. Furthermore, significant positive correlations were found between trophic levels (TLs) and log-transformed concentrations of As (P < 0.001, R2 = 0.20) and Cr (P < 0.001, R2 = 0.13), indicating biomagnification of these two metals across trophic positions among species. Finally, the human health risk assessment revealed that the consumption of cephalopod, shellfish, and sea cucumber poses a higher risk of adverse effects compared to shrimp and crab., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Liangliang Huang reports financial support was provided by National Natural Science Foundation of China (U20A2087). Liangliang Huang reports financial support was provided by Key Research and Development Program of Guangxi (Guike AB22035050). If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)- Published
- 2024
- Full Text
- View/download PDF
6. Investigation of isomerization and oxidation of astaxanthin in ready-to-eat Litopenaeus vannamei during accelerated storage.
- Author
-
Li N, Fan X, Wang Y, Zhang K, Liu R, Xu Y, Tan Z, Xu W, Zhou D, and Li D
- Subjects
- Animals, Isomerism, Antioxidants chemistry, Shellfish analysis, Stereoisomerism, Xanthophylls chemistry, Oxidation-Reduction, Penaeidae chemistry, Food Storage
- Abstract
Astaxanthin (AST), the natural pigment in Litopenaeus vannamei, is susceptible to oxidation and isomerization, leading to the fading of the orange-red color in ready-to-eat (RTE) shrimps. This study specifically investigated the changes mechanism in AST content, including geometric and stereoisomers, as well as oxidation degradation, throughout the storage process of RTE shrimps. The results showed that the total amount of AST decreased by 46.76 % after 45 days of storage at 40 °C. The levels of geometric isomers (all-E, 9-Z, 13-Z) and stereoisomers (3S,3'S, 3S,3'R, 3R,3'R) gradually decreased over time. Notably, 9-Z and 3S,3'S isomers, known for their strong antioxidant activity, were reduced by 83.57 % and 61.64 % respectively. Additionally, AST underwent oxidative degradation, forming short-chain compounds (astaxanthinal or astaxanthinone), with the main products being Apo-14'-astaxanthinal and Apo-7-astaxanthinone DHA ester. These findings provide a theoretical foundation for further research on the degradation mechanism of AST, and offer valuable insights into the color protection of RTE shrimps., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
7. Assessing methods for detecting Alexandrium catenella (Dinophyceae) and paralytic shellfish toxins in Southeast Alaska.
- Author
-
Cornett JC, Cates RJ, Ledger KJ, Pinger CW, Hart CE, Laboda KR, Larson WA, and Hollarsmith JA
- Subjects
- Alaska, Animals, Harmful Algal Bloom, Shellfish Poisoning, Saxitoxin analysis, Biological Assay methods, Dinoflagellida, Marine Toxins analysis, Environmental Monitoring methods, Shellfish analysis
- Abstract
Blooms of Alexandrium catenella threaten to disrupt subsistence, recreational, and commercial shellfish harvest in Alaska, as the paralytic shellfish toxins (PSTs) produced pose a serious public health risk and can lead to costly shutdowns for shellfish farmers. Current methods of PST detection in the region range from monitoring programs utilizing net tows to detect A. catenella to direct shellfish tissue testing via mouse bioassay (MBA) for commercial aquaculture harvest, as well as various optional testing methods for subsistence and recreational harvesters. The efficacy and feasibility of these methods vary, and they have not been directly compared in Southeast Alaska. In this study, we sought to assess and compare A. catenella and PST early detection methods to determine which can provide the most effective and accurate warning of A. catenella blooms or PST events. We found microscope counts to be variable and prone to missing lower numbers of A. catenella, which may be indicative of bloom formation. However, quantitative polymerase chain reaction (qPCR) significantly correlated with microscope counts and was able to effectively detect even low numbers of A. catenella on all sampling days. Paralytic shellfish toxin concentrations measured by enzyme-linked immunosorbent assay and MBA significantly correlated with each other, qPCR, and some microscope counts. These results show that qPCR is an effective tool for both monitoring A. catenella and serving as a proxy for PSTs. Further work is needed to refine qPCR protocols in this system to provide bloom warnings on an actionable timescale for the aquaculture industry and other shellfish harvesters. Integr Environ Assess Manag 2024;20:2189-2202. © 2024 The Authors. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC). This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA., (© 2024 The Authors. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC). This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.)
- Published
- 2024
- Full Text
- View/download PDF
8. In situ investigation of ice fractions and water states during partial freezing of pork loins and shrimps.
- Author
-
Zhu Z, Zhang H, Liu X, Zeng Q, Sun DW, and Wang Z
- Subjects
- Animals, Swine, Magnetic Resonance Spectroscopy, Shellfish analysis, Food Preservation methods, Food Handling, Seafood analysis, Freezing, Ice analysis, Water chemistry, Water analysis, Penaeidae chemistry
- Abstract
Ice fractions and water states in partially frozen muscle foods greatly affect their quality. In the study, a variable temperature nuclear magnetic resonance (VT-NMR) with a liquid nitrogen temperature control system was employed to in situ investigate the relationship between ice fractions and temperatures and changes in water states during partial freezing and thawing of pork and shrimp. Results indicated that changes in ice fractions ranging from -2 ∼ -20 °C could be divided into 3 stages including slow increase, random leap and remarkable leap. More serious damages to the structures related to immobile water occurred in shrimp than in pork, and partial freezing also caused deterioration in muscle fibres related to free water. Additionally, -2 ∼ -3 °C and - 3.5 °C were the appropriate partial freezing temperatures for pork and shrimp, respectively. Therefore, the VT-NMR method possessed great potential for fundamental studies and applications of partial freezing of muscle foods., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
9. Glycogen and zinc-enriched ferritin as bioavailable nanoparticulate nutrients released from gastrointestinal digestion of pacific oyster (Crassostrea gigas).
- Author
-
Li S, Li Y, Dou M, Zhang M, Zhao Z, Wu H, Zhu S, and Obadina AO
- Subjects
- Animals, Humans, Caco-2 Cells, Mice, Nanoparticles chemistry, Nanoparticles metabolism, Gastrointestinal Tract metabolism, Biological Availability, Shellfish analysis, Zinc metabolism, Ferritins metabolism, Ferritins chemistry, Crassostrea metabolism, Crassostrea chemistry, Digestion, Glycogen metabolism
- Abstract
Oyster is a low-carbon animal food enriched with protein, glycogen, and trace minerals. Nano-nutrients are increasingly perceived as an unignorable part of foods. Here, simulated gastrointestinal digestion released a considerable amount of nanoparticulate nutrients from raw and cooked oysters. They were identified as glycogen monomers with size of 20-40 nm and their aggregates, as well as 6 nm-sized bare cores of ferritin containing iron and zinc (4:1, w/w). FITC-labeling and flow cytometry unveiled the efficient uptake of oyster glycogen by polarized Caco-2 cells via macropinocytosis and receptor-mediated endocytosis. Calcein-fluorescence-quenching assay revealed divalent-metal-transporter-1- and macropinocytosis-mediated enterocyte iron absorption from oyster ferritin. Zinquin-fluorescence flow cytometry and ex-vivo mouse ileal loop experiments demonstrated the ready intestinal zinc absorption from oyster ferritin via macropinocytosis, as well as the good resistance of oyster ferritin to phytate's inhibition on zinc absorption. Overall, our results offer a new insight into the digestive and chemical properties of oysters., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
10. The impact of climate change on Omega-3 long-chain polyunsaturated fatty acids in bivalves.
- Author
-
Tan K, Ransangan J, Tan K, and Cheong KL
- Subjects
- Animals, Aquaculture, Shellfish analysis, Nutritive Value, Bivalvia chemistry, Bivalvia metabolism, Fatty Acids, Omega-3 analysis, Climate Change
- Abstract
Omega-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA) have many health benefits to human. Increasing evidence have shown that climate change reduces the availability of plankton n-3 LC-PUFA to primary consumers which potentially reduces the availability of n-3 LC-PUFA to human. Since marine bivalves are an important source of n-3 LC-PUFA for human beings, and bivalve aquaculture completely depends on phytoplankton in ambient water as food, it is important to understand the impact of climate change on the lipid nutritional quality of bivalves. In this study, fatty acid profile of different bivalves (mussels, oysters, clams, scallops and cockles) from different regions (tropical, subtropical and temperate) and time (before 1990, 1991-1995, 1996-2000, 2001-2005, 2006-2010, 2011-2015, 2016-2020) were extracted from published literature to calculate various lipid nutritional quality indicators. The results of this study revealed that the effects of global warming and declines in aragonite saturation state on the lipid content and lipid indices of bivalves are highly dependent on the geographical region and bivalves. In general, global warming has the largest negative impact on the lipid content and indices of temperate bivalves, including decreasing the PUFA/SFA, EPA + DHA and n-3/n-6. However, global warming has a much smaller negative impact on lipid content and lipid indices in other regions. The declines of aragonite saturation state in seawater promotes the accumulation of lipid content in tropical and subtropical bivalves, but it compromised the PUFA/SFA, EPA + DHA and n-3/n-6 of bivalves in all regions. The findings of this study not only fill the knowledge gap of the impact of climate change on the lipid nutritional quality of bivalves, but also provide guidance for the establishment of bivalve aquaculture and fisheries management plans to mitigate the impact of climate change.
- Published
- 2024
- Full Text
- View/download PDF
11. Effect of the farming site and harvest time on the nutritional, elemental and volatile profile of mussels: A comprehensive analysis of the PDO 'Cozza di Scardovari'.
- Author
-
Bordignon F, Aprea E, Betta E, Xiccato G, and Trocino A
- Subjects
- Animals, Shellfish analysis, Mytilus chemistry, Mytilus metabolism, Mytilus growth & development, Aquaculture, Bivalvia chemistry, Bivalvia metabolism, Bivalvia growth & development, Volatile Organic Compounds chemistry, Volatile Organic Compounds analysis, Volatile Organic Compounds metabolism, Nutritive Value, Fatty Acids analysis, Fatty Acids chemistry, Fatty Acids metabolism
- Abstract
This study comprehensively characterised a protected designation of origin mussel 'Cozza di Scardovari' (Mytilus galloprovincialis) by examining how it is affected by the farming site (outer vs. inner area of the lagoon) and harvest time (21 April vs. 18 May vs. 16 June). Harvest time affected the marketable traits and fatty acid profile of mussels, whereas farming site scarcely affected marketable traits and mussel yields. Mussels from the inner area of the lagoon displayed a superior nutritional profile, including higher contents of proteins (7.8% vs. 7.4%; P < 0.05), lipids (1.2% vs. 1.0%; P < 0.001) and essential amino acids such as tryptophan (+24%; P < 0.05) and valine (+8%; P < 0.05), with a more favourable n-3/n-6 ratio (7.7 vs. 7.0; P < 0.001) than those from the close-to-sea area. Volatile organic compounds, mainly octanoic acid, dimethyl sulphide and 1-penten-3-ol, differed between farming sites within the same lagoon., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
12. Simultaneous monitoring of multiple prohibited drugs in various aquatic products.
- Author
-
Nian Q, Meng E, Li F, Wang C, Zhang Q, Li J, and Xu Q
- Subjects
- Animals, Chromatography, High Pressure Liquid, Adsorption, Water Pollutants, Chemical analysis, Water Pollutants, Chemical chemistry, Brachyura chemistry, Solid Phase Extraction methods, Fishes, Food Contamination analysis, Tandem Mass Spectrometry, Seafood analysis, Shellfish analysis
- Abstract
Both sedative and antipathogenic drugs are often found to be illegally used in aquaculture, but there is a lack of simultaneous monitoring methods. A method for simultaneously monitoring multiple prohibited drugs in various aquatic product samples was developed in this work, including fish, shrimp, crab, and shellfish. Sulfonic acid-functionalized magnetic graphitic carbon nitride (S-MGCN) was synthesized and validated to efficiently co-extract all targets (adsorption efficiency over 90.07%) through various adsorption mechanisms such as electrostatic interaction, hydrogen bonding, and π-π interaction while demonstrating good sample matrix purification ability (matrix effect below 13.60%). A new magnetic solid-phase extraction method based on S-MGCN was subsequently established. Coupled with UPLC-MS/MS, the detection limits were 0.030.075 μg /kg, and the recoveries ranged from 88.76% to 111.74% with the RSDs lower than 14.60%, indicating that the developed method has good sensitivity, accuracy, and precision. Further validation of its practicality was achieved through actual sample analysis., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
13. Optimizing color enhancement in dried Penaeus vannamei through high-humidity hot air impingement cooking: Enzyme inactivation, reduced drying time, and Maillard reaction inhibition.
- Author
-
Chen T, Gao Y, Tan Z, Zeshan A, Li J, Ai Z, Mowafy S, Lin Y, and Li X
- Subjects
- Animals, Catechol Oxidase chemistry, Catechol Oxidase metabolism, Desiccation, Shellfish analysis, Antioxidants chemistry, Xanthophylls chemistry, Penaeidae chemistry, Penaeidae enzymology, Maillard Reaction, Cooking, Hot Temperature, Color
- Abstract
This study was aimed to evaluate the potential of high-humidity hot air impingement cooking (HHAIC) on Penaeus vannamei, focusing on its drying characteristics, microstructure, water distribution, enzyme activity, astaxanthin content, antioxidant capacity, color, and Maillard reaction. Results demonstrated that a 3 min HHAIC significantly improved the shrimp's color and optimized astaxanthin content with a notable increase in scavenging capacity based on an in-vitro as antioxidation activity evaluation. Compared to the untreated samples, HHAIC could significantly inactivate polyphenol oxidase by 95.76%. Also, it suppressed the Maillard reaction by decreasing 5-hydroxymethylfurfural content and shortened the drying time by 40%. In addition, the low-field nuclear magnetic resonance and microstructure analysis showed alterations in the shrimp muscle fiber structure and water distribution. This study indicated that HHAIC could elevate quality, enhance appearance, and reduce the processing time of dried shrimp, presenting valuable implications for industry progress., Competing Interests: Declaration of competing interest The authors confirm no conflicts of interest., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
14. Both Hemocyanin and β-1,3-Glucan-Binding Protein from the Shrimp Shell of Litopenaeus vannamei Are Responsible for Its Color Change from Brown to Red during Thermal Processing.
- Author
-
Guan L, Ji R, Zang J, Zhang T, Lv C, and Zhao G
- Subjects
- Animals, Shellfish analysis, Lectins chemistry, Lectins metabolism, Arthropod Proteins chemistry, Arthropod Proteins metabolism, Carrier Proteins chemistry, Carrier Proteins metabolism, Hemocyanins chemistry, Hemocyanins metabolism, Penaeidae chemistry, Penaeidae metabolism, Hot Temperature, Color, Animal Shells chemistry, Animal Shells metabolism
- Abstract
Because of the composition and structural complexity of crustacean shells, their color change mechanism during thermal processing remains unclear. This study identified and characterized two intrinsic protein components, hemocyanin (Lv-Hc) and β-1,3-glucan-binding protein (Lv-BGBP) from Litopenaeus vannamei shrimp shells by a combination of ion-exchange chromatography, gel filtration, and mass spectrometry. It was found that a mixture of Lv-Hc, a gray protein, and Lv-BGBP (which is a natural astaxanthin-binding protein with a red color) is responsible for the brown color of fresh shrimp shells. Upon heating to 100 °C, the mixture of these proteins turned red, mimicking the color change observed in cooked shrimp shells. This transition is attributed to the extremely high thermal stability of Lv-BGBP, which has the ability to protect astaxanthin from thermal induced degradation. These findings provide significant insights into the molecular mechanism governing shrimp shell coloration, advancing our understanding of crustacean biochemistry.
- Published
- 2024
- Full Text
- View/download PDF
15. Elucidation of high-pressure processing toward microbial inhibition, physicochemical properties, collagen fiber and muscle structure of blood clam edible portion.
- Author
-
Palamae S, Patil U, Suyapoh W, Sornying P, Buatong J, Zhang B, and Benjakul S
- Subjects
- Animals, Pressure, Shewanella chemistry, Shewanella metabolism, Food Handling, Shellfish analysis, Shellfish microbiology, Vibrio chemistry, Muscles chemistry, Bivalvia chemistry, Bivalvia microbiology, Collagen chemistry
- Abstract
Impact of high-pressure processing (HP-P) on microbial inactivation, protein oxidation, collagen fiber, and muscle structure of the edible portion (EP) of blood clams (BC) was investigated. Aerobic plate count, Vibrio parahaemolyticus, V. vulnificus, other Vibrio spp. and Shewanella algae counts were not detectable when HP-P pressure of ≥300 MPa was applied. Carbonyl, disulphide bond content, and surface hydrophobicity upsurged as HP-P with augmenting pressure was employed. Protein with ∼53 kDa appeared when HP-P at 100 and 200 MPa was implemented. Increased pressure enhanced gap formation and abnormal muscle cell structure arrangements. HP-P also affected connective tissue, causing size reduction and disruption of the collagen filament fibers. However, firmness and toughness of BC-EP with HP-P ≤ 300 MPa were comparable to those of the control. HP-P at 300 MPa was therefore appropriate for treatment of BC with maintained textural properties, while less protein oxidation, collagen fiber and muscle structure disruption occurred., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
16. Effect of non-enzymatic browning on oysters during hot air drying process: Color and chemical changes and insights into mechanisms.
- Author
-
Hu Y, Zeng X, Jiang K, Luo Y, Quan Z, Li J, Ma Y, Guo X, Zhou D, and Zhu B
- Subjects
- Animals, Shellfish analysis, Oxidation-Reduction, Kinetics, Phenols chemistry, Food Handling, Desiccation methods, Maillard Reaction, Ostreidae chemistry, Hot Temperature, Color
- Abstract
Hot air drying (HAD) is an extensive method used on oysters and it causes the most intuitive change, a color change. However, the mechanism of color change remains unclear. This study showed that oysters underwent browning during the HAD process. The colorimetric parameter L* decreased while a* and b* increased, all of which were well described by the first-order color kinetic model. Mechanistically, the HDA process induced the oxidative browning of phenols and the generation of Maillard reaction products (5-hydroxymethylfurfural and hydrophilic pyrrole). Meanwhile, the HAD process caused lipid oxidation, leading to the reduction of phosphatidylethanolamine and the generation of reactive carbonyl compounds (aldehydes and α-dicarbonyl compounds). Moreover, the accumulation of hydrophobic pyrroles, a lipid-induced Maillard-like reaction product, was observed. These results suggest that, in addition to phenolic oxidation, sugar- and amino acid-mediated non-enzymatic browning reactions, lipid-mediated Maillard-like reactions play important roles in oyster darkening during the HAD process., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
17. Spatiotemporal distribution of lipophilic shellfish toxins in plankton and shellfish in the offshore regions of Shandong province, China.
- Author
-
Wu G, Qiu J, Yan G, Li A, Xu X, Wang X, and Li D
- Subjects
- China, Animals, Spatio-Temporal Analysis, Seasons, Food Contamination analysis, Polyether Toxins, Furans, Macrolides, Marine Toxins analysis, Phytoplankton, Shellfish analysis, Okadaic Acid analysis, Okadaic Acid analogs & derivatives, Pyrans analysis, Zooplankton, Environmental Monitoring
- Abstract
Lipophilic shellfish toxins (LSTs) threaten the ecosystem health and seafood safety. To comprehensively investigate the spatiotemporal distribution of common LSTs in phytoplankton, zooplankton and economic shellfish, three cruises were conducted in five typical offshore aquaculture regions of Shandong province, China, including Haizhou Bay, Jiaozhou Bay, Sanggou Bay, Sishili Bay and Laizhou Bay, in spring (March-April), summer (July-August) and autumn (November-December). This study revealed significant variability in the composition and content of LSTs in phytoplankton samples collected from different regions. Pectenotoxin-2 (PTX2), dinophysistoxin-1 (DTX1) and okadaic acid (OA) were mainly detected in the ranges of not detected (nd)-5045 pmol g
-1 dry weight (dw), nd-159 pmol g-1 dw, and nd-154 pmol g-1 dw, respectively. In zooplankton, DTX1 and OA were the predominant components of LSTs, with the highest levels of ∑LSTs in spring ranging from nd to 406 pmol g-1 dw. Spearman's correlation analysis between LSTs and environmental factors indicated significant correlations for the contents of homo-yessotoxin (hYTX), gymnodimine-A (GYM-A), and spirolide-1 (SPX1) with these factors. Totally relatively low levels of LSTs with dominative DTX1 were detected in economic shellfish, which showed a low risk to seafood safety for human health., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)- Published
- 2024
- Full Text
- View/download PDF
18. Effects of 'bask in sunlight and dewed at night' on the formation of fermented flavor in shrimp paste after maturation.
- Author
-
Gao R, Xue J, Shi T, Li Y, and Yuan L
- Subjects
- Animals, Penaeidae chemistry, Penaeidae growth & development, Penaeidae metabolism, Penaeidae microbiology, Shellfish analysis, Shellfish microbiology, Odorants analysis, Gas Chromatography-Mass Spectrometry, Food Handling, Fermentation, Sunlight, Volatile Organic Compounds chemistry, Volatile Organic Compounds metabolism, Taste, Flavoring Agents chemistry, Flavoring Agents metabolism
- Abstract
The purpose of the study was to illustrate the roles of three primary indexes, namely sunlight, ventilation and stirring, in the 'bask in sunlight and dewed at night' technique on the quality of shrimp paste, through a laboratory-scale design. The results showed that changes in the post-ripening fermentation conditions, especially sunlight, was instrumental in the physicochemical properties of the shrimp paste. E-nose and SPME-GC-MS were employed to assess the volatile flavor of post-ripening fermentation. A total of 29 key volatile aroma components played a crucial role in the development of post-ripening flavor in shrimp paste with or without sunlight. Lipidomic analysis revealed that sunlight promoted the oxidative degradation of FA, resulting in the production of a diverse range of flavor compounds that imparted the unique aroma of shrimp paste. The findings of this study will establish a theoretical basic for better control of the post-ripening fermentation of traditional shrimp paste., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier Ltd.)
- Published
- 2024
- Full Text
- View/download PDF
19. Occurrence, accumulation, ecological risk, and source identification of potentially toxic elements in multimedia in a subtropical bay, Southeast China.
- Author
-
Wang W, Huo Y, Lin C, Lian Z, Wang L, Liu Y, Sun X, Chen J, and Lin H
- Subjects
- China, Animals, Risk Assessment, Aquatic Organisms, Crustacea, Shellfish analysis, Bioaccumulation, Water Pollutants, Chemical analysis, Geologic Sediments analysis, Geologic Sediments chemistry, Bays, Environmental Monitoring, Seawater chemistry, Seawater analysis, Fishes
- Abstract
Potentially toxic elements (PTEs) in seawater and sediments may be amplified along the aquatic food chain, posing a health threat to humans. This study comprehensively analyzed the concentrations, distribution, potential sources, and health risk of 7 PTEs in multimedia (seawater, sediment and organism) in typical subtropical bays in southern China. The results indicated that Zn was the most abundant element in seawater, and the average concentration of Cd in sediment was 3.93 times higher than the background value. Except for As, the seasonal differences in surface seawater were not significant. The content of Zn in fishes, crustacea, and shellfish was the highest, while the contents of Hg and Cd were relatively low. Bioaccumulation factor indicated that Zn was a strongly bioaccumulated element in seawater, while Cd was more highly enriched by aquatic organisms in sediment. According to principal component analysis (PCA), and positive matrix factorization (PMF), the main sources of PTEs in Quanzhou Bay were of natural derivation, industrial sewage discharge, and agricultural inputs, each contributing 40.4 %, 24.2 %, and 35.4 %, respectively. This study provides fundamental and significant information for the prevention of PTEs contamination in subtropical bays, the promotion of ecological safety, and the assessment of human health risk from PTEs in seafood., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
20. Rapid and sensitive detection of domoic acid in shellfish using a magnetic bead-based competitive ELISA with a high-affinity peptide as a molecular binder.
- Author
-
Kim JH, Cho CH, Park TJ, and Park JP
- Subjects
- Animals, Limit of Detection, Bivalvia chemistry, Food Contamination analysis, Kainic Acid analogs & derivatives, Kainic Acid analysis, Enzyme-Linked Immunosorbent Assay methods, Shellfish analysis, Peptides chemistry, Peptides analysis, Marine Toxins analysis
- Abstract
Addressing the critical health concerns posed by domoic acid (DA), a neurotoxic compound produced by toxic marine algae and bioaccumulated in shellfish, necessitates the development of a rapid, precise, and robust detection system. Traditional DA detection methods have stability and sensitivity issues, which hinder effective toxin detection. To overcome these limitations, we developed a novel direct competitive enzyme-linked immunosorbent assay (dc-ELISA) platform that utilizes peptide-immobilized magnetic beads (MGBs/peptide). The affinity peptides identified through phage display and chemically synthesized with biotin labels present an innovative alternative to conventional antibodies for ELISA applications. Streptavidin-modified MGBs were used as the bioreceptor carriers to facilitate magnetic separation and simplify sample preparation, making the MGB/peptide-based dc-ELISA platform an ideal tool for comprehensive monitoring efforts. The developed platform exhibits a detection range of 0.5-10 ng mL
-1 and a low limit of detection of 0.29 ng mL-1 , offering enhanced sensitivity and cost-effectiveness. Moreover, our developed dc-ELISA demonstrated a high recovery rate when validated with DA-spiked CRM-mussel samples. This method overcomes the limitations of traditional detection techniques and offers a scalable and efficient approach to marine toxin surveillance with improved marine environmental monitoring and public health management., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)- Published
- 2024
- Full Text
- View/download PDF
21. Preparation of a colorimetric hydrogel indicator reinforced with modified aramid nanofiber employing natural anthocyanin to monitor shrimp freshness.
- Author
-
Tang Q, Hu J, Liu F, Gui X, and Tu Y
- Subjects
- Animals, Hydrogen-Ion Concentration, Alginates chemistry, Color, Ipomoea batatas chemistry, Penaeidae chemistry, Plant Extracts chemistry, Shellfish analysis, Anthocyanins chemistry, Anthocyanins analysis, Hydrogels chemistry, Nanofibers chemistry, Colorimetry methods
- Abstract
The pH-responsive hydrogels have potential applications in food visualization detection, but their fragile mechanical properties limit their applicability. The excellent mechanical properties and thermal stability of aramid nanofibers (ANFs) can improve the structural stability of hydrogels. In this study, the surface properties of ANFs were enhanced through modification to improve their surface activity. The modified ANFs, designated as ANF-SN, were produced following treatment with a mixture of sulfuric acid (H
2 SO4 ) and nitric acid (HNO3 ), which led to increased reactivity and dispersibility of the ANFs due to the proliferation of active groups on their nanofiber surface. The preferred anthocyanin extract from purple sweet potatoes (purple sweet potato extract [PSPE]) had significant color responses to pH (2-12) and ammonia vapor. A stable dual-network colorimetric hydrogel was fabricated by combining ANF-SN, polyvinyl alcohol/sodium alginate (PVA/SA), and PSPE through a two-step method (freeze-thawing and staining). Characterization analysis showed that the strong acid modification of ANFs effectively improved their chemical reactivity. ANF-SN was better than ANF in promoting the formation of hydrogen bond networks, enhancing hydrogel network structures, and improving the viscoelasticity of hydrogels. The optimal hydrogel indicator PVA/SA/ANF-SN/PSPE had good color responsiveness and sensitivity to ammonia. It can also be used to further determine shrimp freshness value using a smartphone and RGB color-picking software., (© 2024 Institute of Food Technologists.)- Published
- 2024
- Full Text
- View/download PDF
22. Geographical traceability of flavor compounds in Chinese mitten crab (Eriocheir sinensis): Implications for quality differentiation, authenticity assessment, and mechanism research.
- Author
-
Li J, Nie Z, Hu J, Wang L, Song C, Xu D, Gao J, Xu P, and Xu G
- Subjects
- Animals, China, Flavoring Agents chemistry, Flavoring Agents analysis, Gas Chromatography-Mass Spectrometry, Quality Control, Taste, Geography, Hepatopancreas chemistry, Brachyura chemistry, Brachyura classification, Shellfish analysis
- Abstract
Geographical traceability plays a crucial role in ensuring quality assurance, brand establishment, and the sustainable development of the crab industry. In this study, we examined the possibility of using gas chromatography-ion mobility spectrometry with multivariate statistical authenticity analysis to identify the origin of crabs from five sites downstream of the Yangtze River. Significant variations were observed in the levels of alcoholic flavor compounds in the hepatopancreas and muscles of crabs from different geographical locations, and a support vector machine exhibited discriminant ability with 100% accuracy. These flavor variations exhibited significant correlations with the types and concentrations of elements within the crabs, as well as with free amino acids. This study offers a practical approach for determining the geographical traceability of Chinese mitten crabs and elucidates the role of elements in flavor modulation, thereby providing innovative strategies to enhance the efficiency of crab farming., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
23. Development of metabolomic biomarkers to discriminate the geographical origin of Korean and Russian snow crabs using CE-TOF/MS.
- Author
-
Shin J, Yang J, Kim H, Sim Y, Cha E, and Yang JY
- Subjects
- Animals, Russia, Republic of Korea, Mass Spectrometry, Discriminant Analysis, Shellfish analysis, Metabolomics, Biomarkers analysis, Biomarkers metabolism, Brachyura chemistry, Brachyura metabolism, Brachyura classification, Electrophoresis, Capillary
- Abstract
The quantity of snow crabs (Chionoecetes opilio) harvested in Korea is subject to seasonal restrictions; therefore, snow crabs are imported from Russia. Metabolites in snow crabs from two geographic origins were compared. The metabolites were subjected to metabolomic analysis to prevent fraudulent sales of marine products from a particular country. Capillary electrophoresis time-of-flight mass spectrometry was used. Seventy-seven target metabolites were identified using a mass spectral library. Through orthogonal partial least squares discriminant analysis, the top 25 biomarker candidates were evaluated based on p-values and fold changes. A total of 246 peaks (187 and 59 in the cation and anion modes, respectively) were identified. Among the biomarker candidates, 2-oxovaleric acid, asymmetric dimethylarginine, hypotaurine, and allo-threonine were selected as final biomarkers to unequivocally determine the geographic origin. Overall, metabolic analyses allowed us to differentiate snow crabs from different geographic origins. This method could also be extended of other marine products., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
24. A smartphone-assisted sensing hydrogels based on UCNPs@SiO 2 -phenol red nanoprobes for detecting the pH of aquatic products.
- Author
-
Cao Y, Song Y, Fan X, Ma L, Feng T, Zeng J, Xue C, and Xu J
- Subjects
- Hydrogen-Ion Concentration, Animals, Seafood analysis, Nanoparticles chemistry, Shellfish analysis, Colorimetry, Hydrogels chemistry, Silicon Dioxide chemistry, Smartphone, Fishes, Phenolsulfonphthalein chemistry
- Abstract
For some aquatic products, pH has been considered a useful index to reflect the changes in materials during the loss of freshness. Based on the inner filter effect (IFE) between deprotonated phenol red (PR) and upconversion nanoparticles (UCNPs), UCNPs coated with PR-doped SiO
2 shell were embedded in agarose hydrogel to develop a smartphone-assisted method for pH sensing. With the enhancement of pH response using a phase transfer agent (i.e., tetra butyl ammonium hydroxide, TBAH), the proposed senor realized the colorimetric and fluorescence detection of pH in the range of pH 6.6-8 and pH 6-8, respectively. The sensor also showed satisfied reversibility when switched between pH 6 and 8 for at least 5 cycles. Moreover, this sensor displayed great sensitivity, stability, and portability in analyzing actual fish, shrimp, and shellfish samples, providing a new sight for evaluating the freshness of aquatic products., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)- Published
- 2024
- Full Text
- View/download PDF
25. Integrated metabolomics analysis of chill-stored rose shrimp (Parapenaeus longirostris) treated with different pressure levels of high hydrostatic pressure by 1 H-NMR spectroscopy.
- Author
-
Lan Q, Pinheiro ACAS, Braschi G, Picone G, Rocculi P, and Laghi L
- Subjects
- Animals, Seafood microbiology, Seafood analysis, Food Storage methods, Penaeidae microbiology, Penaeidae metabolism, Proton Magnetic Resonance Spectroscopy methods, Metabolome, Bacteria metabolism, Bacteria classification, Shellfish microbiology, Shellfish analysis, Methylamines metabolism, Methylamines analysis, Metabolomics methods, Hydrostatic Pressure, Food Preservation methods
- Abstract
The antimicrobial effects of high hydrostatic pressure (HHP) treatments on chill-stored seafood are well-documented, while their impact on the metabolic profile of seafood, especially the metabolome of fish flesh, and remains underexplored. Addressing this gap, this study investigates the effects of HHP on the metabolome of chill-stored rose shrimp by conducting multivariate data analysis based on untargeted proton nuclear magnetic resonance observations. Vacuum-packed rose shrimp samples were subjected to HHP at 0, 400, 500, and 600 MPa for 10 min and then stored at 2-4°C. The microorganism analysis and metabolic analysis were carried out on days 1 and 14. HHP treatment effectively deactivated Lactobacillus spp., Escherichia coli, Pseudomonas spp., total Coliforms, and sulfite-reducing anaerobic bacteria. Consequently, HHP treatment significantly reduced the formation rate of decay-related metabolites, such as hypoxanthine, trimethylamine, and biogenic amines, which exhibited significant accumulation in untreated samples. Multivariate unsupervised analyses provided insights into the overall changes in the metabolite profile induced by HHP. Metabolic pathway analysis revealed several pathways underlying spoilage, including pyruvate metabolism, valine, leucine, and isoleucine biosynthesis, purine metabolism, methane metabolism, glycine, serine, and threonine metabolism, citrate cycle (TCA cycle), glycolysis/gluconeogenesis, alanine, aspartate, and glutamate metabolism, sulfur metabolism, pantothenate and CoA biosynthesis, glutathione metabolism, and glyoxylate and dicarboxylate metabolism. Importantly, these pathways underwent alterations due to the application of HHP, particularly at high-pressure levels. In summary, the results unveil the potential mechanisms of HHP effects on chill-stored rose shrimps., (© 2024 The Author(s). Journal of Food Science published by Wiley Periodicals LLC on behalf of Institute of Food Technologists.)
- Published
- 2024
- Full Text
- View/download PDF
26. Effect of baking on the structure and bioavailability of protein-binding zinc from oyster (Crassoetrea hongkongensis).
- Author
-
Song C, Zhong R, Zeng S, Chen Z, Tan M, Zheng H, Gao J, Lin H, Zhu G, and Cao W
- Subjects
- Humans, Caco-2 Cells, Animals, Cooking, Carrier Proteins chemistry, Carrier Proteins metabolism, Hot Temperature, Protein Binding, Shellfish analysis, Biological Availability, Zinc metabolism, Zinc chemistry, Ostreidae chemistry, Ostreidae metabolism
- Abstract
To compare the bioavailability of protein-binding zinc, we investigated the impact of baking on the structure of zinc-binding proteins. The results showed that zinc-binding proteins enriched in zinc with relative molecular weights distributed at 6 kDa and 3 kDa. Protein-binding zinc is predisposed to separate from proteins' interiors and converge on proteins' surface after being baked, and its structure tends to be crystalline. Especially -COO, -C-O, and -C-N played vital roles in the sites of zinc-binding proteins. However, baking did not affect protein-binding zinc's bioavailability which was superior to that of ZnSO
4 and C12 H22 O14 Zn. They were digested in the intestine, zinc-binding complexes that were easily transported and uptaken by Caco-2 cells, with transport and uptake rates as high as 62.15% and 15.85%. Consequently, baking can alter the conformation of zinc-binding proteins without any impact on protein-binding zinc's bioavailability which is superior to that of ZnSO4 and C12 H22 O14 Zn., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)- Published
- 2024
- Full Text
- View/download PDF
27. Effects of different cooking methods on the proximate composition and physical properties of Brown shrimp ( Metapenaeus dobsonii) during cooking and freezing cycle.
- Author
-
Verma SK, Ganesan P, Kishore P, Remya S, Mohan CO, Padmavathy P, Muralidhran N, and Bindu J
- Subjects
- Animals, Hot Temperature, Shellfish analysis, Microwaves, Water analysis, Seafood analysis, Cooking methods, Freezing, Penaeidae chemistry, Color
- Abstract
Present study aimed to evaluate the changes in proximate composition and physical attributes in brown shrimp ( Metapenaeus dobsonii ) exposed to different methods of cooking followed by freezing. For this, three different grades (100/200, 200/300, and 300/500 numbers per kg) of brown shrimp were cooked at 90 °C till the core temperature of the product reaches 85 °C using hot water, steam, and microwave (400 W) techniques. The changes in yield, cooking loss, proximate composition, textural, and colour profile were assessed for cooked shrimps. The cooking loss was higher for larger grades of shrimp, whereas shrimp cooked using hot water exhibited the highest cooking loss. Lowest cooking loss was observed for microwave-cooked shrimp. Moisture content decreased after cooking whereas protein, fat, ash, and calorie content increased. After cooking, different grades of shrimp showed an increase in their lightness (L*), redness (a*), and yellowness (b*) values. The smaller grade shrimp exhibited lower value for cohesiveness, hardness, chewiness, and gumminess. Different cooking techniques yielded cooked shrimp of varying hardness values., Competing Interests: DECLARATION OF CONFLICTING INTERESTSThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
- Published
- 2024
- Full Text
- View/download PDF
28. Amine response smartphone-based portable and intelligent polyvinyl alcohol films for real-time detection of shrimp freshness.
- Author
-
Luo M, Liu J, Zhang Y, Wang T, Ren X, Gui L, Zhao J, Zhang X, Tang Y, Zeng Z, Hou F, Zhong Q, Yuan Z, and Xu H
- Subjects
- Animals, Amines chemistry, Amines analysis, Penaeidae chemistry, Shellfish analysis, Curcumin chemistry, Curcumin analysis, Smartphone, Polyvinyl Alcohol chemistry, Food Packaging instrumentation
- Abstract
Food freshness monitoring is an important component in ensuring food safety for consumers and the food industry. Therefore, there is an urgent need for a portable, low-cost, and efficient detection method to determine the freshness. In this study, polyvinyl alcohol (PVA) was used as polymer carrier to prepare electrospinning film containing curcumin (Cur) and gardenia blue (GB) as intelligent indicator label on food packaging for real-time nondestructive detection of freshness of shrimp. The detection limit of ammonia response is less than or equal to 20 ppm, and the detection time is about 1 min, indicating that it has a sensitive response effect. At the same time, a smartphone application that can identify amines in response to color changes has been developed, and consumers can understand freshness by scanning the label. This study demonstrates the huge potential of smart indicator labels for food freshness monitoring., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
29. Insight into the correlation of key taste substances and key volatile substances from shrimp heads at different temperatures.
- Author
-
Liu Z, Wei S, Xiao N, Liu Y, Sun Q, Zhang B, Ji H, Cao H, and Liu S
- Subjects
- Animals, Food Storage, Volatile Organic Compounds chemistry, Taste, Penaeidae chemistry, Amino Acids chemistry, Amino Acids analysis, Temperature, Shellfish analysis
- Abstract
This study aimed to investigate taste substances of shrimp heads stored at 20 °C, 4 °C, -3 °C, and - 18 °C, and the correlation between taste substances and 25 key volatile substances. Notably, samples stored at 20 °C showed significant changes in bitter amino acids and hypoxanthine, and quickly deteriorated. Samples stored at 4 °C for 14 d or - 3 °C for 30 d facilitated the development of umami amino acids, sweet amino acids, and IMP. Furthermore, samples stored at -18 °C for 30 d demonstrated no significant changes in taste profile. Changes in taste substances through quantitative analysis were consistent with changes in taste profile through e-tongue analysis. Based on the results of O2PLS (VIP > 1), Cys, Arg, Glu, Ser, Val, Ala, Ile, ADP, and IMP were correlated with 25 key volatile substances. This study provides fundamental data for the storage, transportation, and value-added utilization of shrimp heads., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
30. Molecular mechanism of color deepening of ready-to-eat shrimp during storage.
- Author
-
Li N, Fan X, Chen T, Wang Y, Tan Z, Liu C, Zhou D, and Li D
- Subjects
- Animals, Penaeidae chemistry, Oxidation-Reduction, Fast Foods analysis, Phenols chemistry, Food Storage, Color, Maillard Reaction, Shellfish analysis
- Abstract
Color deepening occurs during storage of ready-to-eat (RTE) shrimps, which seriously affects their marketing cycle. This study investigated the molecular mechanisms of color deterioration in RTE shrimps during accelerated storage, shedding light on the pattern of change in colored products and content. The findings revealed significant occurrences of phenolic oxidation, lipid oxidation, and Maillard browning reactions during accelerated storage. Qualitative and quantitative analyses were conducted on the colored products resulting from these chemical reactions. Multivariate mathematical models were employed to analyze the phenolic oxidation products (2-methylanthraquinone and p-benzoquinone), lipid oxidation products (lipofuscin-like pigments and hydrophobic pyrroles), and Maillard browning products (pyrazines and melanoidins). These products were identified as the main contributors to the deepening of the color of RTE shrimps during storage. The outcomes of this research could enhance our understanding of the color change mechanism in thermally processed marine foods, providing valuable insights for quality maintenance and industrial advancement., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
31. Effects of different thermal sterilization conditions on the quality of ready-to-eat shrimp based on specific sterilization intensity.
- Author
-
Wang S, Lin S, Li S, Qian X, Li C, and Sun N
- Subjects
- Animals, Fast Foods analysis, Oxidation-Reduction, Food Handling, Digestion, Sterilization, Penaeidae chemistry, Shellfish analysis, Hot Temperature
- Abstract
The effects of different thermal sterilization conditions on the quality and digestibility of ready-to-eat (RTE) shrimp were investigated. Compared with the high-temperature (121 °C) and short-time (6 min and 8 min) sterilization, the low-temperature (110 and 115 °C) and long-time (>20 min) sterilization significantly promoted the Maillard and browning reactions and changed the color of the RTE-shrimp. The high sterilization temperature promoted shrimp protein oxidation, resulting in increased carbonyl group, disulfide bond, and free radical content, while the free sulfhydryl group content decreased. This oxidation and tissue destruction at high temperature led to reduced texture properties and altered water distribution within the shrimp's muscles. However, sterilized shrimp exhibited superior digestive properties in an in vitro simulated digestion experiment. High-temperature and short-time sterilization is more effective in mitigating the quality deterioration of RTE-shrimp compared to low-temperature and long-time sterilization., Competing Interests: Declaration of competing interest The authors declare no competing financial interest., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
32. Controlled Spread of a Ag Layer from the Core to the Tip along the Branches of AuAg Nanostars for Improved SERS Detection of Okadaic Acid in Shellfish.
- Author
-
Li YL, Zhu J, Weng GJ, Li JJ, and Zhao JW
- Subjects
- Animals, Polyethyleneimine chemistry, Limit of Detection, Aptamers, Nucleotide chemistry, Food Contamination analysis, Silver chemistry, Spectrum Analysis, Raman methods, Gold chemistry, Okadaic Acid analysis, Shellfish analysis, Metal Nanoparticles chemistry
- Abstract
Plasmonic Au-Ag nanostars are excellent surface-enhanced Raman scattering (SERS) probes due to bimetallic coupling and the tip effect. However, the existing preparation methods of AuAg nanostars cannot achieve controlled growth of the Ag layer on the branches of nanostars and so cannot display their SERS to the maximum extent, thus limiting its sensitivity in biosensing. Herein, a novel strategy "PEI (polyethylenimine)-guided Ag deposition method" is proposed for synthesizing AuAg core-shell nanostars (AuAg@Ag NS) with a tunable distribution of the Ag layer from the core to the tip, which offers an avenue for investigating the correlation between SERS efficiency and the extent of spread of the Ag layer. It is found that AuAg@Ag NS with a Ag layer coated the whole branch has the strongest SERS performance because the coupling between the tips and Ag layer is maximized. Meanwhile, as a completely closed core-shell structure, AuAg@Ag NS can confine and anchor 4-ATP inside the Ag layer to avoid an unstable SERS signal. By connecting the aptamer, a reliable internal standard nanoprobe with a SERS enhancement factor (EF) up to 1.86 × 10
8 is prepared. Okada acid is detected through competitive adsorption of this SERS probes, and the detection limit is 36.6 pM. The results gain fundamental insights into tailoring the nanoparticle morphologies and preparation of internal standard nanoprobes and also provide a promising avenue for marine toxin detection in food safety.- Published
- 2024
- Full Text
- View/download PDF
33. Identification and validation of core microbes for the formation of the characteristic flavor of fermented oysters (Crassostrea gigas).
- Author
-
Liu L, Liu T, Wang H, Zhao Y, Xu X, and Zeng M
- Subjects
- Animals, Gas Chromatography-Mass Spectrometry, Odorants analysis, Saccharomyces cerevisiae metabolism, Saccharomyces cerevisiae chemistry, Fungi metabolism, Fungi classification, Volatile Organic Compounds metabolism, Volatile Organic Compounds chemistry, Volatile Organic Compounds analysis, Shellfish analysis, Shellfish microbiology, Fermentation, Crassostrea microbiology, Crassostrea metabolism, Crassostrea chemistry, Flavoring Agents metabolism, Flavoring Agents chemistry, Bacteria metabolism, Bacteria classification, Bacteria isolation & purification, Taste
- Abstract
Self-fermented oyster homogenates were prepared to investigate core microbes and their correlations with flavor formation mechanisms. Five bacterial and four fungal genera were identified. Correlation analysis showed that Saccharomyces cerevisiae, Kazachstania, and L. pentosus were core species for the flavor of fermented products. Four core microbes were selected for inoculation into homogenates. Twelve key aroma compounds with odor activity values >1 were identified by gas chromatography-mass spectrometry. L. plantarum and S. cerevisiae were beneficial for producing key aroma compounds such as 1-octen-3-ol, (E,Z)-2,6-nonadienal, and heptanal. Fermentation with four microbes resulted in significant increases in contents of Asp, Glu, Lys, inosine monophosphate, and guanosine monophosphate, which provided freshness and sweetness. Fermentation with four microbes resulted in high digestibility, antioxidant abilities, and zinc contents. This study has elucidated the mechanism of flavor formation by microbial action and provides a reference for targeted flavor control in fermented oyster products., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
34. Metabolomic insights into the effects of seasonal shifts on the dynamic variation of non-volatile compounds of abalone (Haliotis discus hannai).
- Author
-
Tian HH, Li YL, Wang XS, Huang XH, Zhang YY, Zhang ZC, Zhou DY, and Qin L
- Subjects
- Animals, Lipids chemistry, Nutritive Value, Male, Female, Seasons, Gastropoda chemistry, Gastropoda metabolism, Shellfish analysis, Metabolomics, Amino Acids metabolism, Amino Acids analysis, Amino Acids chemistry
- Abstract
Abalone (Haliotis spp.) is a shellfish known for its exceptional nutritional value and significant economic worth. This study investigated the dynamic characteristics of non-volatile compounds over a year, including metabolites, lipids, nucleotides, and free amino acids (FAAs), which determined the nutritional quality and flavor of abalone. 174 metabolites and 371 lipids were identified and characterized, while 20 FAAs and 11 nucleotides were quantitatively assessed. These non-volatile compounds of abalone were fluctuated with months variation, which was consistent with the fluctuations of environmental factors, especially seawater temperature. Compared with seasonal variation, gender had less influence on these non-volatiles. June and July proved to be the optimal harvesting periods for abalone, with the levels of overall metabolites, lipids, FAAs, and nucleotides in abalone exhibiting a higher value in June and July over a year. Intriguingly, taurine covered 60% of the total FAAs and abalone could be used as dietary taurine supplementation., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
35. Microencapsulation of ginger essential oil using mung bean protein isolate-chitosan complex coacervates: Application in the preservation of crab meatballs and the prediction of shelf life.
- Author
-
Shen J, Zhang M, and Yang C
- Subjects
- Animals, Plant Proteins chemistry, Brachyura chemistry, Brachyura microbiology, Shellfish analysis, Shellfish microbiology, Chitosan chemistry, Oils, Volatile chemistry, Oils, Volatile pharmacology, Food Preservation methods, Food Storage, Zingiber officinale chemistry, Drug Compounding, Vigna chemistry, Vigna growth & development
- Abstract
Crab meatballs with more unsaturated fat tend to spoil. Ginger essential oil (GEO) with oxidation resistance was encapsulated into microcapsules (GM) by complex cohesion of mung bean protein isolate (MBPI) and chitosan (CS) in a ratio of 8:1 at pH = 6.4, encapsulation efficiency (EE) and payload (PL) of GM (D
50 = 26.16 ± 0.45 μm) with high thermal stability were 78.35 ± 1.02% and 55.43 ± 0.64%. GM (0.6%, w/w) did not interfere with the original flavor of crab meatballs, and lowered values of pH, thiobarbituric acid reactive substances (TBARS) and total bacteria counts (TBC) of the products than those spiked with GEO and the control. The prediction accuracy of the logistic first-order growth kinetic equation in line with TBC (2.84%) was better than that of zero-order and Arrhenius coupled equation based on pH (7.48%) and TBARS (5.94%), but all of them could predict the shelf life of crab meatballs containing GM stored at 4-25 °C., Competing Interests: Declaration of competing interest There are no conflicts to declare., (Copyright © 2024 Elsevier Ltd. All rights reserved.)- Published
- 2024
- Full Text
- View/download PDF
36. Effects of Glycosylation Combined with Phosphate Treatment on the Allergenicity and Structure of Tropomyosin in Litopenaeus vannamei .
- Author
-
Sun QF, Xia F, Li MS, Zhang HL, Liao YN, Liu QM, Liu M, Chen GX, Luo LZ, and Liu GM
- Subjects
- Animals, Female, Humans, Mice, Arthropod Proteins immunology, Arthropod Proteins chemistry, Food Hypersensitivity immunology, Glycosylation, Immunoglobulin G immunology, Immunoglobulin G chemistry, Mice, Inbred BALB C, Shellfish analysis, Shellfish Hypersensitivity immunology, Th2 Cells immunology, Th2 Cells drug effects, Allergens immunology, Allergens chemistry, Immunoglobulin E immunology, Penaeidae immunology, Penaeidae chemistry, Phosphates chemistry, Tropomyosin immunology, Tropomyosin chemistry
- Abstract
Tropomyosin (TM) is the main allergen in shrimp ( Litopenaeus vannamei ). In this study, the effects of allergenicity and structure of TM by glycosylation (GOS-TM), phosphate treatment (SP-TM), and glycosylation combined with phosphate treatment (GOS-SP-TM) were investigated. Compared to GOS-TM and SP-TM, the IgG/IgE binding capacity of GOS-SP-TM was significantly decreased with 63.9 ± 2.0 and 49.7 ± 2.7%, respectively. Meanwhile, the α-helix content reduced, surface hydrophobicity increased, and 10 specific amino acids (K
30 , K38 , S39 , K48 , K66 , K74 , K128 , K161 , S210 , and K251 ) were modified by glycosylation on six IgE linear epitopes of GOS-SP-TM. In the BALB/c mice allergy model, GOS-SP-TM could significantly reduce the levels of specific IgE, IgG1, and CD4+ IL-4+ , while the levels of IgG2a, CD4+ CD25+ Foxp3+ , and CD4+ IFN-γ+ were increased, which equilibrated Th1 and Th2 cells, thus alleviating allergic symptoms. These results indicated that glycosylation combined with phosphate treatment can provide a new insight into developing hypoallergenic shrimp food.- Published
- 2024
- Full Text
- View/download PDF
37. Development of a Quick and Highly Sensitive Amplified Luminescent Proximity Homogeneous Assay for Detection of Saxitoxin in Shellfish.
- Author
-
Zhao C, Zhang Z, Li J, Lu Y, Ma F, Wang Z, Geng J, Huang B, and Qin Y
- Subjects
- Animals, Limit of Detection, Food Contamination analysis, Fluorescence Resonance Energy Transfer, Saxitoxin analysis, Shellfish analysis, Luminescent Measurements methods
- Abstract
Saxitoxin (STX), an exceptionally potent marine toxin for which no antidote is currently available, is produced by methanogens and cyanobacteria. This poses a significant threat to both shellfish aquaculture and human health. Consequently, the development of a rapid, highly sensitive STX detection method is of great significance. The objective of this research is to create a novel approach for identifying STX. Therefore, amplified luminescent proximity homogeneous assay (AlphaLISA) was established using a direct competition method based on the principles of fluorescence resonance energy transfer and antigen-antibody specific binding. This method is sensitive, rapid, performed without washing, easy to operate, and can detect 8-128 ng/mL of STX in only 10 min. The limit of detection achieved by this method is as low as 4.29 ng/mL with coefficients of variation for the intra-batch and inter-batch analyses ranging from 2.61% to 3.63% and from 7.67% to 8.30%, respectively. In conclusion, our study successfully establishes a simple yet sensitive, rapid, and accurate AlphaLISA method for the detection of STX which holds great potential in advancing research on marine biotoxins.
- Published
- 2024
- Full Text
- View/download PDF
38. Spatial distribution of lipophilic shellfish toxins in seawater and sediment in the Bohai Sea and the Yellow Sea, China.
- Author
-
Li D, Qiu J, Wang X, Li A, Wu G, Yin C, and Yang Y
- Subjects
- China, Shellfish analysis, Water Pollutants, Chemical analysis, Seasons, Animals, Oceans and Seas, Macrolides analysis, Polyether Toxins, Furans, Seawater chemistry, Geologic Sediments chemistry, Marine Toxins analysis, Okadaic Acid analysis, Okadaic Acid analogs & derivatives, Environmental Monitoring, Pyrans analysis
- Abstract
Lipophilic shellfish toxins (LSTs) are widely distributed in marine environments worldwide, potentially threatening marine ecosystem health and aquaculture safety. In this study, two large-scale cruises were conducted in the Bohai Sea and the Yellow Sea, China, in spring and summer 2023 to clarify the composition, concentration, and spatial distribution of LSTs in the water columns and sediments. Results showed that okadaic acid (OA), dinophysistoxin-1 (DTX1) and/or pectenotoxin-2 (PTX2) were detected in 249 seawater samples collected in spring and summer. The concentrations of ∑LSTs in seawater were ranging of ND (not detected) -13.86, 1.60-17.03, 2.73-17.39, and 1.26-30.21 pmol L
-1 in the spring surface, intermediate, bottom water columns and summer surface water layers, respectively. The detection rates of LSTs in spring and summer seawater samples were 97% and 100%, respectively. The high concentrations of ∑LSTs were mainly distributed in the north Yellow Sea and the northeast Bohai Sea in spring, and in the northeast Yellow Sea, the waters around Laizhou Bay and Rongcheng Bay in summer. Similarly, only OA, DTX1 and PTX2 were detected in the surface sediments. Overall, the concentration of ∑LSTs in the surface sediments of the northern Yellow Sea was higher than that in other regions. In sediment cores, PTX2 was mainly detected in the upper sediment samples, whereas OA and DTX1 were detected in deeper sediments, and LSTs can persist in the sediments for a long time. Overall, OA, DTX1 and PTX2 were widely distributed in the water column and surface sediments in the Bohai Sea and the Yellow Sea, China. The results of this study contribute to the understanding of spatial distribution of LSTs in seawater and sediment environmental media and provide basic information for health risk assessment of phycotoxins., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)- Published
- 2024
- Full Text
- View/download PDF
39. Toward Sensitive and Reliable Immunoassays of Marine Biotoxins: From Rational Design to Food Analysis.
- Author
-
Ji Y, Wang R, and Zhao H
- Subjects
- Immunoassay methods, Animals, Food Analysis methods, Humans, Shellfish analysis, Fishes, Seafood analysis, Marine Toxins analysis, Food Contamination analysis
- Abstract
Marine biotoxins are metabolites produced by algae that can accumulate in shellfish or fish and enter organisms through the food chain, posing a serious threat to biological health. Therefore, accurate and rapid detection is an urgent requirement for food safety. Although various detection methods, including the mouse bioassay, liquid chromatography-mass spectrometry, and cell detection methods, and protein phosphatase inhibition assays have been developed in the past decades, the current detection methods cannot fully meet these demands. Among these methods, the outstanding immunoassay virtues of high sensitivity, reliability, and low cost are highly advantageous for marine biotoxin detection in complex samples. In this work, we review the recent 5-year progress in marine biotoxin immunodetection technologies such as optical immunoassays, electrochemical immunoassays, and piezoelectric immunoassays. With the assistance of immunoassays, the detection of food-related marine biotoxins can be implemented for ensuring public health and preventing food poisoning. In addition, the immunodetection technique platforms including lateral flow chips and microfluidic chips are also discussed. We carefully investigate the advantages and disadvantages for each immunoassay, which are compared to demonstrate the guidance for selecting appropriate immunoassays and platforms for the detection of marine biotoxins. It is expected that this review will provide insights for the further development of immunoassays and promote the rapid progress and successful translation of advanced immunoassays with food safety detection.
- Published
- 2024
- Full Text
- View/download PDF
40. Occurrence of microplastics in the Haima cold seep area of the South China Sea.
- Author
-
Zhang D, Li J, Ju P, Cao W, Jiang F, and Sun C
- Subjects
- China, Geologic Sediments chemistry, Shellfish analysis, Animals, Plastics analysis, Microplastics analysis, Environmental Monitoring, Water Pollutants, Chemical analysis, Seawater chemistry
- Abstract
The pollution of deep-sea microplastics has received increasing attention. As a special ecosystem in the deep sea, the cold seep area is of great significance for studying the distribution of microplastics in the deep sea. In this work, the distribution and characteristics of microplastics in seawater, sediments, and shellfish in the Haima cold seep area and the correlation between the characteristics of microplastics in different media and the type of media were studied. Microplastics were found in all three media. The abundance of microplastics in different samples from the Haima cold seep area ranged 1.8-3.8 items/L for the seawater, 11.47-96.8 items/kg (d.w.) for the surface sediments, and 0-5 items/individual (0-0.714 items/g) for the shellfish. The amount of microplastics ingested by shellfish varied among different species. The microplastics in these three media were mainly fibrous, dark-colored, small-sized rayon, polyethylene terephthalate (PET), and polyethylene (PE). In the correlation analysis of microplastic characteristics among the three media, it was found that the characteristics of microplastics in different media in the same area were closely related, and each pair of variables showed a significant positive correlation (P ≤ 0.05). The distinctive geographical conditions would accelerate the interchange of microplastics among various media. Principal component analysis showed that habitat contribute to microplastic feature differences in shellfish. Differences in correlation were observed between the characteristics of shellfish microplastics in different regions and the characteristics of microplastics in surrounding seawater and sediments., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
41. Structural and Compositional Changes in Two Marine Shell Traditional Chinese Medicines: A Comparative Analysis Pre- and Post-Calcination.
- Author
-
Wu L, Liu C, Yao T, Shi Y, Shen J, Gao X, and Qin K
- Subjects
- Animals, Arcidae chemistry, Animal Shells chemistry, Principal Component Analysis, Shellfish analysis, Trace Elements analysis, Trace Elements chemistry, Medicine, Chinese Traditional, Calcium Carbonate chemistry, Calcium Carbonate analysis
- Abstract
Background: Arcae concha and Meretricis concha cyclinae concha are two marine shellfish herbs with similar composition and efficacy, which are usually calcined and used clinically., Objective: This study investigated variations in the inorganic and organic components of Arcae concha and Meretricis concha cyclinae concha from different production regions, both Arcae concha and Meretricis concha cyclinae concha. The aim was to enhance the understanding of these two types of marine shell traditional Chinese medicine (msTCM) and provide a foundation for their future development and application., Method: Spectroscopic techniques, including infrared spectroscopy, X-ray spectroscopy, and X-ray fluorescence spectroscopy, were used to analyze the calcium carbonate (CaCO3) crystal and trace elements. Thermogravimetric analysis was used to investigate the decomposition process during heating. The proteins were quantified using the BCA protein assay kit. Principal component analysis (PCA) was used to classify inorganic elements in the two marine shellfish traditional Chinese medicines., Results: No significant differences were found among the various production regions. The crystal structure of CaCO3 in the raw products was aragonite, but it transformed into calcite after calcination. The contents of Ca, Na, Sr, and other inorganic elements were highest. The protein content was significantly reduced after calcination. Therefore, these factors cannot accurately reflect the internal quality of TCM, rendering qualitative identification challenging. CaCO3 dissolution in the decoction of Arcae concha and Meretricis concha cyclinae concha increased after calcination, aligning with the clinical application of calcined shell TCM. PCA revealed the inorganic elements in them, indicating that the variation in trace element composition among different drugs leads to differences in their therapeutic focus, which should be considered during usage., Conclusions: This study clarifies the composition and structure changes of corrugated and clam shell before and after calcining, and it lays the foundation for the comprehensive utilization of marine traditional Chinese medicine., Highlights: These technical representations reveal the differences between raw materials and processed products, which will provide support for the quality control of other shellfish TCM., (© The Author(s) 2024. Published by Oxford University Press on behalf of AOAC INTERNATIONAL. All rights reserved. For permissions, please email: journals.permissions@oup.com.)
- Published
- 2024
- Full Text
- View/download PDF
42. Gelatin/dextran active films incorporated with cinnamaldehyde and α-tocopherol for scallop (Patinopecten yessoensis) adductor muscle preservation.
- Author
-
Fan F, Yue C, Zhai Z, Liao H, Lian X, and Xie H
- Subjects
- Animals, Antioxidants pharmacology, Permeability, Shellfish analysis, Hydrophobic and Hydrophilic Interactions, Gelatin chemistry, Pectinidae chemistry, Acrolein analogs & derivatives, Acrolein pharmacology, Acrolein chemistry, Dextrans chemistry, Dextrans pharmacology, alpha-Tocopherol pharmacology, alpha-Tocopherol chemistry, Food Preservation methods, Food Packaging methods
- Abstract
Scallops are rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid but perishable due to their microbial growth and lipid oxidation. In this study, gelatin/dextran films containing cinnamaldehyde and α-tocopherol (0% + 0%, 0.3% + 0.3%, 0.6% + 0.6%, 0.9% + 0.9%, and 1.2% + 1.2%, w/w) as active fillers were developed by solution casting method, and their preservation effects on scallop adductor muscle refrigerated at 4°C for 0, 3, 6, 9, and 12 days were evaluated. Inclusion of the two active fillers did not influence the thermal stability of the films but created heterogenous and discontinuous film microstructure and increased the film hydrophobicity. Increase in the concentrations of active fillers lowered the mechanical properties and water vapor permeability of the films but increased their crystallinity, thickness, water contact angle, opacity, antibacterial property, and antioxidant property. The longest release times for both cinnamaldehyde and α-tocopherol were found in 95% (v/v) ethanol solution. The gelatin/dextran films containing 1.2% (w/w) of active fillers (Gelatin [Ge]/Dextran [Dx]/1.2 film) improved the chemical stability of refrigerated scallop adductor muscle. The total viable count (TVC) of the unpackaged scallop adductor muscle exceeded the recommended limit of 7 lg CFU/g on day 6 (7.07 ± 0.50 lg CFU/g), whereas the TVC of the Ge/Dx/1.2 film-packaged scallop adductor muscle was still below the limit on day 9 (5.60 ± 0.50 lg CFU/g). Thus, the Ge/Dx/1.2 film can extend the shelf life of refrigerated scallop adductor muscle by at least 3 days. Overall, the developed gelatin/dextran active packaging films are promising for the preservation of aquatic food products., (© 2024 Institute of Food Technologists.)
- Published
- 2024
- Full Text
- View/download PDF
43. Abundance of microplastics at and near a shellfish aquaculture farm: An eastern oyster (Crassostrea virginica) transplant study.
- Author
-
Mladinich K, Holohan BA, Shumway SE, and Ward JE
- Subjects
- Animals, Shellfish analysis, Seawater chemistry, Connecticut, Crassostrea, Aquaculture, Water Pollutants, Chemical analysis, Environmental Monitoring, Microplastics analysis
- Abstract
Microplastics (MP) have repeatedly been found in commercially cultured species of bivalves. There are concerns regarding the amount of MP released into the environment by aquaculture activities, and questions regarding possible higher MP loads in farm-grown shellfish compared to levels in shellfish collected from recreational beds. To explore this concept, seawater, aquaculture gear, and eastern oysters (Crassostrea virginica) were sampled from an aquaculture site in Niantic Bay, CT, USA, and a 2-week transplantation experiment was performed in which oysters were transplanted between the aquaculture site and a plastic-free cage off the dock at the University of Connecticut-Avery Point campus. The digestive gland-stomach complex (gut) was dissected from the oysters and MP were extracted from the adjacent seawater and oyster gut samples using previously validated extraction methods. Extensive quality assurance and control measures were taken to reduce MP contamination. Particles in all samples were isolated, imaged under a stereomicroscope, and characterized (size, shape, polymer) using ImageJ software and micro-Fourier transform infrared spectroscopy. Water samples contained 0-0.3 MP/L and oyster gut samples contained 0-1.3 MP/g wet weight indicating very low concentrations of MP at the farm (0-2 MP/individual) or away from the farm (0-3 MP/individual). Aquaculture gear in this area is not contributing to MP ingestion in farmed oysters or elevated MP levels in the surrounding water., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
44. Levels and spatial profile of per- and polyfluoroalkyl substances in edible shrimp products from Japan and neighboring countries; a potential source of dietary exposure to humans.
- Author
-
Fujii Y, Kato Y, Miyatake M, Akeda S, Nagata S, Ando J, Kido K, Ohta C, Koga N, Harada KH, and Haraguchi K
- Subjects
- Japan, Animals, Humans, Food Contamination analysis, Food Contamination statistics & numerical data, Water Pollutants, Chemical analysis, Environmental Monitoring, Sulfonamides analysis, Shellfish analysis, Penaeidae, Seafood analysis, Fluorocarbons analysis, Dietary Exposure statistics & numerical data, Dietary Exposure analysis, Alkanesulfonic Acids analysis
- Abstract
Exposure to per- and polyfluoroalkyl substances (PFAS) is of great concern for human health because of their persistence and potentially adverse effects. Dietary intake, particularly through aquatic products, is a significant route of human exposure to PFAS. We analyzed perfluoroalkyl sulfonic acid (PFSA with carbon numbers from 6 to 8 and 10 (C
6 -C8 , C10 )) and perfluorooctanesulfonamide (FOSA), and perfluoroalkyl carboxylic acid (PFCA with carbon numbers from 6 to 15 (C6 -C15 )) in 30 retail packs of edible shrimps, which included seven species from eight coastal areas of Japan and neighboring countries. The most prevalent compounds were perfluorooctane sulfonate (PFOS, C8 ) and perfluoroundecanoic acid (PFUnDA, C11 ), accounting for 46 % of total PFAS. The concentrations ranged from 6.5 to 44 ng/g dry weight (dw) (equivalent to 1.5 to 10 ng/g wet weight (ww)) and varied according to species and location. For example, Alaskan pink shrimp (Pandalus eous) from the Hokuriku coast, Japan contained high levels of long-chain PFCAs (38 ng/g dw (equivalent to 8.7 ng/g ww)), while red rice prawn (Metapenaeopsis barbata) from Yamaguchi, Japan contained a high concentration of PFOS (29 ng/g dw (equivalent to 6.7 ng/g ww)). We also observed regional differences in the PFAS levels with higher concentrations of long-chain PFCAs in Japanese coastal waters than in the South China Sea. The PFAS profiles in shrimp were consistent with those in the diet and serum of Japanese consumers, suggesting that consumption of seafood such as shrimp may be an important source of exposure. The estimated daily intake of sum of all PFAS from shrimp from Japanese coastal water was 0.43 ng/kg body weight/day in average, which could reach the weekly tolerable values (4.4 ng/kg body weight /week) for the sum of the four PFSA set by the EFSA for heavy consumers. The high concentration of PFAS in shrimp warrants further investigation., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)- Published
- 2024
- Full Text
- View/download PDF
45. Nanozyme-assisted molecularly imprinted polymer-based indirect competitive ELISA for the detection of marine biotoxin.
- Author
-
Cho CH, Kim JH, Padalkar NS, Reddy YVM, Park TJ, Park J, and Park JP
- Subjects
- Humans, Marine Toxins analysis, Molecularly Imprinted Polymers, Gold, Hydrogen Peroxide, Shellfish analysis, Saxitoxin, Enzyme-Linked Immunosorbent Assay methods, Peptides, Polymers, Biosensing Techniques, Metal Nanoparticles, Cobalt, Oxides
- Abstract
Saxitoxin (STX), which is produced by certain dinoflagellate species, is a type of paralytic shellfish poisoning toxin that poses a serious threat to human health and the environment. Therefore, developing a technology for the convenient and cost-effective detection of STX is imperative. In this study, we developed an affinity peptide-imprinted polymer-based indirect competitive ELISA (ic-ELISA) without using enzyme-toxin conjugates. AuNP/Co
3 O4 @Mg/Al cLDH was synthesized by calcining AuNP/ZIF-67@Mg/Al LDH, which was obtained by combining AuNPs, ZIF-67, and flower-like Mg/Al LDH. This synthesized nanozyme exhibited high catalytic activity (Km = 0.24 mM for TMB and 132.5 mM for H2 O2 ). The affinity peptide-imprinted polymer (MIP) was imprinted with an STX-specific template peptide (STX MIP) on a multi-well microplate and then reacted with an STX-specific signal peptide (STX SP). The interaction between the STX SP and MIP was detected using a streptavidin-coated nanozyme (SA-AuNP/Co3 O4 @Mg/Al cLDH). The developed MIP-based ic-ELISA exhibited excellent selectivity and sensitivity, with a limit of detection of 3.17 ng/mL (equivalent: 0.317 μg/g). Furthermore, the system was validated using a commercial ELISA kit and mussel tissue samples, and it demonstrated a high STX recovery with a low coefficient of variation. These results imply that the developed ic-ELISA can be used to detect STX in real samples., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)- Published
- 2024
- Full Text
- View/download PDF
46. Insights into pollution characteristics and human health risks of plasticizer phthalate esters in shellfish species.
- Author
-
Lin X, Lin L, Chang S, Xing Y, Zhang Y, and Yang C
- Subjects
- China, Animals, Humans, Risk Assessment, Environmental Monitoring, Esters analysis, Food Contamination analysis, Phthalic Acids analysis, Plasticizers analysis, Shellfish analysis, Water Pollutants, Chemical analysis
- Abstract
The ubiquitous application of phthalate esters (PAEs) as plasticizers contributes to high levels of marine pollution, yet the contamination patterns of PAEs in various shellfish species remain unknown. The objective of this research is to provide the first information on the pollution characteristics of 16 PAEs in different shellfish species from the Pearl River Delta (PRD), South China, and associated health risks. Among the 16 analyzed PAEs, 13 were identified in the shellfish, with total PAE concentrations ranging from 23.07 to 3794.08 ng/g dw (mean = 514.35 ng/g dw). The PAE pollution levels in the five shellfish species were as follows: Ostreidae (mean = 1064.12 ng/g dw) > Mytilus edulis (mean = 509.88 ng/g dw) > Babylonia areolate (mean = 458.14 ng/g dw) > Mactra chinensis (mean = 378.90 ng/g dw) > Haliotis diversicolor (mean = 335.28 ng/g dw). Dimethyl phthalate (DMP, mean = 69.85 ng/g dw), diisobutyl phthalate (DIBP, mean = 41.39 ng/g dw), dibutyl phthalate (DBP, mean = 130.91 ng/g dw), and di(2-ethylhexyl) phthalate (DEHP, mean = 226.23 ng/g dw) were the most abundant congeners. Notably, DEHP constituted the most predominant fraction (43.98 %) of the 13 PAEs detected in all shellfish from the PRD. Principal component analysis indicated that industrial and domestic emissions served as main sources for the PAE pollution in shellfish from the PRD. It was estimated that the daily intake of PAEs via shellfish consumption among adults and children ranged from 0.004 to 1.27 μg/kg
bw /day, without obvious non-cancer risks (< 0.034), but the cancer risks raised some alarm (2.0 × 10-9 -1.4 × 10-5 ). These findings highlight the necessity of focusing on marine environmental pollutants and emphasize the importance of ongoing monitoring of PAE contamination in seafood., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)- Published
- 2024
- Full Text
- View/download PDF
47. Nutritional and physicochemical characteristics of Asiatic hard clam powder prepared by different cook-drying processes: a comparative study.
- Author
-
Viji P, Binsi PK, Sireesha S, S J L, and Ninan G
- Subjects
- Animals, Shellfish analysis, Food Handling methods, Desiccation methods, Bivalvia chemistry, Nutritive Value, Cooking, Amino Acids analysis, Amino Acids chemistry, Fatty Acids chemistry, Fatty Acids analysis, Powders chemistry
- Abstract
Background: Asiatic hard clam (Meretrix meretrix) is an underutilized bivalve resource. This study discusses dried clam powders prepared from this resource to enhance its utilization and improve nutritional security in protein-deficient populations. Dried clam powder was prepared from Asiatic hard clam and the effects of different pre-cooking methods (boil-dried clam powder, BDCP; steam-dried clam powder, SDCP; and microwave-dried clam powder, MDCP) on nutritional (proximate composition, amino acid profiling, mineral profiling, fatty acid profiling) and physicochemical qualities were investigated., Results: Different pre-cooking methods significantly influenced the characteristics of the clam powder. The MDCP sample showed the highest concentration of amino acids, polyunsaturated fatty acids, Na, K, Ca and Mg content compared to BDCP and SDCP. The boiling process led to a loss of nutritional quality in terms of amino acids and macrominerals. The MDCP displayed the highest solubility in water (30.10%) but its oil and water absorption characteristics were the lowest among all the samples. Boil-cooked clam powder displayed the highest oil binding (2.03 mL g
-1 protein) capacity. Boiling and steaming processes resulted in malondialdehyde generation compared to microwaving. Different pre-cooking processes did not influence the colour attributes significantly, but the control sample prepared without pre-cooking (CCP) had a significantly lower L* value (32.34), resulting in a darker product. In vitro digestibility of the clam powder varied in the order MDCP > SDCP > BDCP > CCP., Conclusion: The study demonstrated that nutritionally rich protein powder can be prepared from Asiatic hard clam. Based on the results, microwave pre-cooking is considered the best pre-cooking method to preserve the nutritional qualities of clam powder. © 2024 Society of Chemical Industry., (© 2024 Society of Chemical Industry.)- Published
- 2024
- Full Text
- View/download PDF
48. Exploring Release, Isomerization, and Absorption of Cypermethrin in Pacific Oysters ( Crassostrea gigas ) with Different Processing Methods during In Vivo Digestion: Insights from a Gastrointestinal Tract Quantitative Tracing Method.
- Author
-
Zhang Y, Shi T, Bao Y, Tan Y, Luo Y, and Hong H
- Subjects
- Animals, Mice, Isomerism, Shellfish analysis, Food Contamination analysis, Humans, Male, Food Handling methods, Pyrethrins metabolism, Pyrethrins analysis, Crassostrea metabolism, Crassostrea chemistry, Digestion, Gastrointestinal Tract metabolism, Insecticides metabolism, Insecticides chemistry
- Abstract
Cypermethrin (CP) is a neurotoxic insecticide found accumulated in oysters, one of the most commonly consumed seafoods, posing potential health risks to the human body. We designed a gastrointestinal tracing method allowing for accurate quantification of the propulsion of chyme and further established the mouse in vivo digestion model to explore the behavior of CP in the digestion of raw, steamed, and roasted oysters. The results showed that bioaccumulation of CP in oysters may be accompanied by the biotransformation of CP. Thermal processing decreased both the CP content in oysters and its bioaccessibility. The small intestine is the main site for CP digestion and absorption. The cis -isomers of CP might finally accumulate in the body at a higher ratio and further become the predominant configuration for toxic effects. Taken together, the study contributes to the risk assessment of the dietary exposure of CP from aquatic products.
- Published
- 2024
- Full Text
- View/download PDF
49. Temperature effects on plasmalogen profile and quality characteristics in Pacific oyster (Crassostrea gigas) during depuration.
- Author
-
Song Y, Hao L, Wang X, Wang X, Cong P, Li Z, Xue C, and Xu J
- Subjects
- Animals, Shellfish analysis, Proteomics methods, Antioxidants metabolism, Antioxidants analysis, Alkaline Phosphatase metabolism, Food Quality, Plasmalogens metabolism, Plasmalogens analysis, Crassostrea genetics, Crassostrea metabolism, Temperature
- Abstract
The quality of Pacific oyster (Crassostrea gigas) can be affected by many factors during depuration, in which temperature is the major element. In this study, we aim to determine the quality and plasmalogen changes in C. gigas depurated at different temperatures. The quality was significantly affected by temperature, represented by varying survival rate, glycogen content, total antioxidant capacity, alkaline phosphatase activity between control and stressed groups. Targeted MS analysis demonstrated that plasmalogen profile was significantly changed during depuration with PUFA-containing plasmalogen species being most affected by temperature. Proteomics analysis and gene expression assay further verified that plasmalogen metabolism is regulated by temperature, specifically, the plasmalogen synthesis enzyme EPT1 was significantly downregulated by high temperature and four plasmalogen-related genes (GPDH, PEDS, Pex11, and PLD1) were transcriptionally regulated. The positive correlations between the plasmalogen level and quality characteristics suggested plasmalogen could be regarded as a quality indicator of oysters during depuration., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
50. Applications of high-intensity ultrasound on shrimp: Potential, constraints, and prospects in the extraction and retrieval of bioactive compounds, safety, and quality.
- Author
-
Marques LP, Bernardo YAA, and Conte-Junior CA
- Subjects
- Animals, Sonication methods, Xanthophylls analysis, Food Safety methods, Polysaccharides analysis, Ultrasonic Waves, Penaeidae chemistry, Penaeidae microbiology, Food Handling methods, Shellfish analysis, Shellfish microbiology
- Abstract
The global shrimp market holds substantial prominence within the food industry, registering a significant USD 24.7 billion in worldwide exportation in 2020. However, the production of a safe and high-quality product requires consideration of various factors, including the potential for allergenic reactions, occurrences of foodborne outbreaks, and risks of spoilage. Additionally, the exploration of the recovery of bioactive compounds (e.g., astaxanthin [AX], polyunsaturated fatty acids, and polysaccharides) from shrimp waste demands focused attention. Within this framework, this review seeks to comprehend and assess the utilization of high-intensity ultrasound (HIUS), both as a standalone method and combined with other technologies, within the shrimp industry. The objective is to evaluate its applications, limitations, and prospects, with a specific emphasis on delineating the impact of sonication parameters (e.g., power, time, and temperature) on various applications. This includes an examination of undesirable effects and identifying areas of interest for current and prospective research. HIUS has demonstrated promise in enhancing the extraction of bioactive compounds, such as AX, lipids, and chitin, while concurrently addressing concerns such as allergen reduction (e.g., tropomyosin), inactivation of pathogens (e.g., Vibrio parahaemolyticus), and quality improvement, manifesting in reduced melanosis scores and improved peelability. Nonetheless, potential impediments, particularly related to oxidation processes, especially those associated with lipids, pose a hindrance to its widespread implementation, potentially impacting texture properties. Consequently, further optimization studies remain imperative. Moreover, novel applications of sonication in shrimp processing, including brining, thawing, and drying, represent a promising avenue for expanding the utilization of HIUS in the shrimp industry., (© 2024 Institute of Food Technologists.)
- Published
- 2024
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.