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Abstract The transcriptional control of sporulation in Bacillus subtilis is reasonably well understood, but its translational control is underexplored. Here, we use RNA-seq, ribosome profiling and fluorescence microscopy to study the translational dynamics of B. subtilis sporulation. We identify two events of translation silencing and describe spatiotemporal changes in subcellular localization of ribosomes during sporulation. We investigate the potential regulatory role of ribosomes during sporulation using a strain lacking zinc-independent paralogs of three zinc-dependent ribosomal proteins (L31, L33 and S14). The mutant strain exhibits delayed sporulation, reduced germination efficiency, dysregulated translation of metabolic and sporulation-related genes, and disruptions in translation silencing, particularly in late sporulation.

Zirconium alloys are used as a cladding material for fuel elements in nuclear reactors. In the case of severe accident conditions, the possible rapid oxidation of zirconium in steam or/and air may result in intense hydrogen generation and hydrogen–oxide mixture explosion. Advanced technologies for increasing the corrosion resistance of claddings are being investigated in two directions: (a) protective coatings on Zr alloys and (b) the use of new materials for claddings. Coatings with silicon may provide a more protective barrier than the ZrO2 films formed on an alloy cladding during nuclear plant operations. These coatings may also serve as a protective barrier during high-temperature accidents. The current work aimed at developing protective coatings with silicon on zirconium alloys. Multielemental Zr–Si–Cr coatings were formed on Zry-2 alloy using the physical vapor deposition (PVD) method. Long-term oxidation tests were carried out under the following conditions: 360°C/195 bar/63 days/water-simulating PWR water. Obtained results show the protective character of formed layers. The material in the form of silicon carbide grains covered with yttrium–aluminum garnet (SiC + YAG) was prepared using the sol–gel method. The formed powder is the main component for coating formation on Zr–1Nb alloy using the method of suspension plasma spraying (SPS).

This article explores the challenges and methods related to developing fluency in a foreign language for professional purposes at university. It deals with the difficulties faced by both teachers and students in maneuvring through linguistic complexities, overcoming language barriers and mastering specialized terminology relevant to various professional fields. The article highlights the importance of a targeted approach that fosters not only linguistic proficiency but also a deep understanding of cultural distinctions and communication norms, specific for various industries. Additionally, it investigates pedagogical strategies and methods aimed at enhancing fluency acquisition at classes of foreign language for professional purposes. Key challenges in the process of developing fluency at classes of foreign language for professional purposes have been highlighted: linguistic complexity; language barriers; cultural nuances; motivation and engagement; limited practice; fear of making mistakes; individual learning styles and preferences; assessment and feedback. Possible methods for developing fluency have been considered: communicative language teaching; immersion into a targeted language; organizing language exchanges; using authentic materials; role-playing and simulation; fluency-oriented activities, unassisted repeated reading and audio-assisted repeated reading; digital storytelling; vlogging, etc. By highlighting challenges and a certain number of existing methods of fostering fluency development, the article stresses on the necessity to provide regular feedback and correction of mistakes. Creating a favorable, engaging, interactive, and stress-free learning environment in the classroom has been stressed as the key prerequisite.

Introduction, aim: At present, tooth autotransplantation is considered one of the therapeutic methods for the replacement of lost teeth. In the last 30 years, the method of tooth autotransplantation has been developed and refined and has become a basic knowledge of dental practitioners. The aim of this clinical retrospective study was to examine children and adult patients with tooth autotransplantation and obtain survival and success rates. Another aim was to determine other specifics of autotransplantation in each group with conclusions relevant for clinical practice. Methods: The study population consisted of the patients referred consecutively for tooth autotransplantation to a specialist department between years 2016 and 2021. The specialization of the referring dentist, the suitability and feasibility of the transplantation, and the donor and recipient area of the tooth graft were recorded. In 2022, clinical and radiological evaluation of the autotransplanted teeth was performed. Patients were divided into two groups according to age in time of autotransplantation, namely under 18 years and over 18 years. Results: Overall, 73 patients in the age range of 10-59 years were referred for autotransplantation. The mean age was 21.43 years with a median age of 17 years. Autotransplantation was not recommended in 12 patients. A total of 68 autotransplantations were performed, but at the time of examination, 12 did not meet the minimum 6-month follow up, so they were excluded from the evaluation. A total of 56 autotransplants were evaluated. In the group of children, there were 27 patients aged 10-17 years who underwent autotransplantation of at least one permanent tooth. A total of 34 teeth were transplanted. The evaluation of the transplanted teeth was performed 6-50 months after the transplantation with a mean follow-up time of 24 months. The survival rate was 100% and the success rate was 91%. The adult group consisted of 22 patients aged 18-59 years and a total of 22 teeth were transplanted. The evaluation of the transplanted teeth was performed 6-72 months after transplantation with a mean of 33 months. The survival rate was 95%, the success rate 77%. Conclusion: The use of autograft to replace undeveloped or lost teeth was most often indicated by orthodontists. In the patients under 18 years of age, the premolars are the predominant donor area and recipient area. In patients over 18 years of age, the third molars are the predominant donor area and the mandibular molars the predominant recipient area. Both age groups have high survival and success rates of autograft and this procedure can be considered as a predictable method of tooth replacement.

Two new negative-parity bands have been identified in the odd-odd 104Rh nucleus. According to their experimentally observed properties, they have the same π(1g9/2)−1⊗ν(1h11/2)1 high-j configuration as the previously known negative-parity chiral doublet bands. This observation raises the possibility of the existence of multiple chiral doublet bands, MχD, in this nucleus. Comparing the properties of the observed bands with results of detailed theoretical calculations, one can conclude that the lower energy parts of bands 1 and 2 are chiral partner bands with the π(1g9/2)−1⊗ν(1h11/2)1 two-quasiparticle configuration, while bands 3 and 4 are chiral partner bands with the π(1g9/2)−1⊗ν(1g7/2)−2(1h11/2)1 four-quasiparticle configuration. Thus, MχD based on different configurations is observed in 104Rh.

Abstract Background Information on social media may affect peoples’ contraceptive decision making. We performed an exploratory analysis of contraceptive content on Twitter (recently renamed X), a popular social media platform. Methods We selected a random subset of 1% of publicly available, English-language tweets related to reversible, prescription contraceptive methods posted between January 2014 and December 2019. We oversampled tweets for the contraceptive patch to ensure at least 200 tweets per method. To create the codebook, we identified common themes specific to tweet content topics, tweet sources, and tweets soliciting information or providing advice. All posts were coded by two team members, and differences were adjudicated by a third reviewer. Descriptive analyses were reported with accompanying qualitative findings. Results During the study period, 457,369 tweets about reversible contraceptive methods were published, with a random sample of 4,434 tweets used for final analysis. Tweets most frequently discussed contraceptive method decision-making (26.7%) and side effects (20.5%), particularly for long-acting reversible contraceptive methods and the depot medroxyprogesterone acetate shot. Tweets about logistics of use or adherence were common for short-acting reversible contraceptives. Tweets were frequently posted by contraceptive consumers (50.6%). A small proportion of tweets explicitly requested information (6.2%) or provided advice (4.2%). Conclusions Clinicians should be aware that individuals are exposed to information through Twitter that may affect contraceptive perceptions and decision making, particularly regarding long-acting reversible contraceptives. Social media is a valuable source for studying contraceptive beliefs missing in traditional health research and may be used by professionals to disseminate accurate contraceptive information.

Selection is a fundamental part of the plant breeding process, enabling the identification and development of varieties with desirable traits. Thanks to advances in genetics and biotechnology, the selection process has become more precise and efficient, resulting in faster breeding progress and better adaptation of crops to environmental challenges. Genetic parameters related to gene additivity and epistasis play a key role and can influence decisions on the suitability of breeding material. In this study, 188 rapeseed doubled haploid lines were assessed in field conditions for resistance to Leptosphaeria spp. Through next-generation sequencing, a total of 133,764 molecular markers (96,121 SilicoDArT and 37,643 SNP) were obtained. The similarity of the DH lines at the phenotypic and genetic levels was calculated. The results indicate that the similarity at the phenotypic level was markedly different from the similarity at the genetic level. Genetic parameters related to additive gene action effects and epistasis (double and triple) were calculated using two methods: based on phenotypic observations only and using molecular marker observations. All evaluated genetic parameters (additive, additive-additive and additive-additive-additive) were statistically significant for both estimation methods. The parameters associated with the interaction (double and triple) had opposite signs depending on the estimation method.

IntroductionSelf-management programs for spinal cord injury (SCI) are a growing adjunct to traditional healthcare services aiding individuals with SCI in learning and managing symptoms and medical care. A benefit of self-management programs is that they can be facilitated by peers, offering a unique lived experience of adjusting to and managing SCI. While a growing body of literature highlights the effectiveness of peer led programs, there is limited understanding of how individuals engage with peer programs or critical components of peer support. The current study seeks to understand how individuals engaged with peers in the context of a self-management program.MethodsSecondary qualitative analysis of online forum posts resultant from a peer led self-management course for SCI.ResultsContent analysis revealed several themes of how participants engaged with members of the group, including skill building, resource sharing, and problem solving. A process level theme of emotional connection to others living with similar SCI-related challenges was defined as “bearing witness.” Participants commented frequently that groups were the first time they engaged with a community with lived experience, and shared experience was frequently highlighted in the responses from individuals as one of the most unique and important aspects of the intervention.DiscussionThemes identified suggest that bearing witness was a critical component of peer led intervention. While self-management content provided structure for engagement and discussion, participants report that connectedness and shared experience made content more impactful and relevant. Future research should examine if alignment of peer and participant experience increases the impact of interventions and explore if this theme is important for other chronic medical populations.

Weld surfacing is the process of applying a layer of metal to the surface of metal objects by simultaneously melting the substrate. As a result of this process, the metal content of the padding weld can be as high as several tens of percents. It is a method used to regenerate machine parts and improve the properties of the surface layer, increasing its resistance to abrasion, corrosion, erosion, and cavitation. It also supports the repair and creation of permanent protective coatings in the engineering, automotive, energy, and aerospace industries. This makes it possible to repair damaged parts instead of completely replacing them, saving time and production costs. Plasma surfacing technology is used for components that require high hardness and corrosion resistance under various environmental conditions. Plasma wire surfacing is not sufficiently presented and described in the current literature, which creates problems in determining the appropriate process parameters. The influence of variable plasma surfacing parameters on steel C45 significantly affects surfacing weld geometry, the dilution factor, and microhardness. Higher currents can increase the dilution factor, integrating more base metal into the weld pool, which may alter the chemical composition and mechanical properties of the weld. Variations in surfacing speed and heat input also affect the microhardness of the surfaced joint, with higher heat inputs potentially leading to softer welds due to slower cooling rates. Optimizing these parameters is essential to achieving desired surfacing weld characteristics and ensuring the structural integrity of C45 steel joints. This paper presents the influence of varying plasma surfacing parameters on the surfacing geometry, the dilution factor, and microhardness. The tests were carried out on a Panasonic TM-1400 GIII automated surfacing machine with CastoMag 45554S solid wire as the filler material. Flat bars of C45 steel were prepared, and then the variable parameters of the surfacing process were developed. Tests were carried out to determine the dilution factor, followed by microhardness measurements. The results showed a significant dependence of the effect of the parameters on the surfacing geometry and the dilution factor.

This paper examines the energy efficiency of three variations of the two-degree-of-freedom transverse galloping energy harvester. These variants differ in the number and placement of electromechanical transducers. By utilizing the harmonic balance method, the limit cycles of mathematical models of the devices were determined. Analytical expressions derived from the models were then used to formulate the efficiency of the systems. It was demonstrated that efficiency depends on flow speed and can be comprehensively characterized by the following criteria parameters: peak efficiency, denoting the maximum efficiency of the system, and high-efficiency bandwidth, which describes the range of flow speeds within which the efficiency remains at no less than 90% of peak efficiency. The values of these parameters are heavily reliant on two other parameters: the speed at which the system achieves peak efficiency, referred to as the nominal speed, and also the flow speed at which the system undergoes Hopf bifurcation, namely the critical speed. Comparative analysis revealed that only the device equipped with two electromechanical transducers can potentially outperform a simple one-degree-of-freedom system. For selected parameters, this gain reached nearly 10%.

In the face of the rapid evolution of the gaming market and the puzzling overlap of genres, consistency in classification seems elusive. The purpose of the present review was to explore the classification of video game genres in the context of psychological research. The aim was to address the challenges associated with creating consistent and meaningful classifications of video game genres, considering the rapid evolution of the gaming market and recent tendency to create games that could be classified into multiple genres. We performed a search in four databases according to the PRISMA guidelines and reviewed 96 full-text papers (N = 49 909). Through our findings, we reveal how researchers strive to classify genres and the numerous complications that arise from this pursuit. In the face of these challenges, we propose alternative ways of classifying genres. Our first proposal is a new classification of video game genres based on our literature review. In our second proposal, we advocate a more detailed understanding by focusing on specific gaming mechanics, and thus we introduce the innovative concept of utilizing community-based tags, such as Steam tags, as an alternative to genres in psychological research.

This paper presents a machine learning based solar power forecast method that can take into account shading related fluctuations. The generated PV power is difficult to predict because there are various fluctuations. Such fluctuations can be weather related when a cloud passes over the array. But they can also occur due to shading caused by stationary obstacles, and this paper addresses this form of shading. In this work an approach is presented that improves the forecast under such fluctuations caused by shading. A correction of the prediction could successfully reduce error due to shading. The evaluation of the model is based on five sets of recorded shading data, where shading resulted from intentionally placed structures. The correction uses internal inverter data and irradiance values of the previous day to perform the correction and was able to reduce the RMSE of four 10 kWp systems with different orientation and tilt angle under shading and thus improve the prediction accuracy by up to 40%. The model can detect how intense the shading is and correct the forecast by itself.

Objective: To determine if the interaction of opiate misuse and marijuana use frequency is associated with behavioral health outcomes. Setting: Community. Participants: Three thousand seven hundred fifty participants enrolled in the Traumatic Brain Injury Model Systems who completed the Pain Survey and had complete opioid use and marijuana use information. Design: Cross-sectional, secondary analysis from a multisite observational cohort. Main Outcome Measure(s): Clinically significant behavioral health symptoms for posttraumatic stress disorder (PTSD), depression, anxiety, and sleep quality. Results: Three thousand five hundred thirty-five (94.3%) participants did not misuse opiates, 215 (5.7%) did misuse opiates (taking more opioid pain medication than prescribed and/or using nonprescription opioid pain medication); 2683 (70.5%) participants did not use marijuana, 353 (9.3%) occasionally used marijuana (less than once a week), and 714 (18.8%) regularly used marijuana (once a week or more frequently). There was a statistically significant relationship (P < .05) between the interaction of opiate misuse and marijuana use frequency and all behavioral health outcomes and several covariates (age, sex, cause of injury, severity of injury, and pain group category). Pairwise comparisons confirm that statistically significant associations on behavioral health outcomes are driven by endorsing opiate misuse and/or regular marijuana use, but occasional marijuana use was not associated. Conclusions: Higher odds of clinically significant PTSD, depression, anxiety, and poor sleep quality are present in people with traumatic brain injury (TBI) who misuse opiates and/or who use marijuana regularly. In the absence of opiate misuse, regular marijuana use had higher odds of worse behavioral health outcomes than occasional and no use. The interaction of opiate misuse and regular marijuana use yielded the highest odds. Individuals with TBI should be informed of the relationship of substance use and behavioral health outcomes and that current chronic pain may mediate the association. [ABSTRACT FROM AUTHOR]

(1) Adapt evidence-based hypnosis-enhanced cognitive therapy (HYP-CT) for inpatient rehabilitation setting; and (2) determine feasibility of a clinical trial evaluating the effectiveness of HYP-CT intervention for pain after spinal cord injury (SCI). Pilot non-randomized controlled trial. Inpatient rehabilitation unit. English-speaking patients admitted to inpatient rehabilitation after SCI reporting current pain of at least 3 on a 0-10 scale. Persons with severe psychiatric illness, recent suicide attempt or elevated risk, or significant cognitive impairment were excluded. Consecutive sample of 53 patients with SCI-related pain enrolled, representing 82% of eligible patients. Up to 4 sessions of HYP-CT Intervention, each 30-60 minutes long. Participants were assessed at baseline and given the choice to receive HYP-CT or Usual Care. Participant enrollment and participation and acceptability of intervention. Exploratory analyses examined the effect of intervention on pain and cognitive appraisals of pain. In the HYP-CT group, 71% completed at least 3 treatment sessions and reported treatment benefit and satisfaction with the treatment; no adverse events were reported. Exploratory analyses of effectiveness found pre-post treatment pain reductions after HYP-CT with large effect (P <.001; β=-1.64). While the study was not powered to detect significant between-group differences at discharge, effect sizes revealed decreases in average pain (Cohen's d =-0.13), pain interference (d =-0.10), and pain catastrophizing (d =-0.20) in the HYP-CT group relative to control and increases in self-efficacy (d =0.27) and pain acceptance (d =0.15). It is feasible to provide HYP-CT to inpatients with SCI, and HYP-CT results in substantial reductions in SCI pain. The study is the first to show a psychological-based nonpharmacologic intervention that may reduce SCI pain during inpatient rehabilitation. A definitive efficacy trial is warranted. [ABSTRACT FROM AUTHOR]