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Transfer critically ill newborn to the operating theater is a very difficult challenge for nurses, anesthesiologists, neonatologists and surgeons. Most of these patients are mechanically ventilated and cardiocirculatory supported. The biggest challenge for premature infants that need to leave the incubator, while outside the Neonatal Intensive Care Unit (NICU), is maintaining temperature homeostasis. A male preterm newborn, born at 28 gestational week with a birth weight of 700 g underwent a major surgical procedure due to a type C esophageal atresia. This case report is focused on two points: the multidisciplinary approach and the preparation of the patient and settings. Favorable logistic and expertise of the staff can make a difference in this process. An hybrid OT combines the advantages of both the bedside surgery and the OT setting.

Determining the chemical quality of soil organic matter and decomposing organisms is critical for predicting carbon fluxes in ecosystems. However, the classical C/N and lignin/N ratios have been criticized for their capability in predicting ecosystem function including litter decay rate, nitrogen mineralization and soil aggregation. Therefore, with our work we aim to complement information on the chemical properties of primary producers and decomposers based on 13C NMR. Overall, 108 unique 13C NMR spectra were collected from higher plant tissues including leaf litter, fine roots, woody debris, as well as a range of microbes i.e. bacteria, fungi, and marine algae. Results showed clear discrimination between organisms belonging to different kingdoms, but with limited variability within bacteria, fungi, and microalgae. In detail, bacteria were found to be distinct not only from plant tissues but also from fungi, characterized by a high proportion of alkyl and carbonyl C types and a rather low relative abundance of O Alkyl C and di-O Alkyl C types. Among the fungi, we found clear separation between yeasts, which are more similar to bacteria, and filamentous species, which are rich in O Alkyl and di-O Alkyl C types. Microalgae are characterized by a high content of aliphatic, carboxylic and methoxyl C fractions. In the spectra of terrestrial plants, the O Alkyl C region, associated with sugars and cellulose, is more abundant compared to algae and bacteria. In addition, the aromatic carbon signal, which is mainly associated with lignin, is much higher for higher plants than in microorganisms. As for plant tissues, woody tissues are clearly different from leaves and fine roots, with grasses, sedges, and ferns being well distinguished from forbs, coniferous and deciduous trees. In summary, our study contains the largest freely available 13C NMR spectral library of bacteria, fungi, algae, and higher plant tissues. However, the data from higher plants are sufficient to cover different functional types, which is not yet possible for bacteria and fungi.

The worm Caenorhabditis elegans, with its short lifecycle and well-known genetic and metabolic pathways, stands as an exemplary model organism for biological research. Its simplicity and genetic tractability make it an ideal system for investigating the effects of different conditions on its metabolism. The chemical analysis of this nematode was performed to identify specific metabolites produced by the worms when fed with either self- or nonself-DNA. A standard diet with OP50 feeding was used as a control. Different development stages were sampled, and their chemical composition was assessed by liquid chromatography–mass spectrometry combined with chemometrics, including both principal component analysis and orthogonal partial least squares discriminant analysis tools. The obtained data demonstrated that self-DNA-treated larvae, when arrested in their cycle, showed significant decreases in dynorphin, an appetite regulator of the nematode, and in N-formyl glycine, a known longevity promoter in C. elegans. Moreover, a substantial decrease was also recorded in the self-DNA-fed adults for the FMRF amide neuropeptide, an embryogenesis regulator, and for a dopamine derivative modulating nematode locomotion. In conclusion, this study allowed for the identification of key metabolites affected by the self-DNA diet, providing interesting hints on the main molecular pathways involved in its biological inhibitory effects.

The cell cycle and the transcriptome dynamics of yeast exposed to extracellular self-DNA during an aerobic batch culture on glucose have been investigated using cytofluorimetric and RNA-seq analyses. In parallel, the same study was conducted on yeast cells growing in the presence of (heterologous) nonself-DNA. The self-DNA treatment determined a reduction in the growth rate and a major elongation of the diauxic lag phase, as well as a significant delay in the achievement of the stationary phase. This was associated with significant changes in the cell cycle dynamics, with slower exit from the G0 phase, followed by an increased level of cell percentage in the S phase, during the cultivation. Comparatively, the exposure to heterologous DNA did not affect the growth curve and the cell cycle dynamics. The transcriptomic analysis showed that self-DNA exposure produced a generalized downregulation of transmembrane transport and an upregulation of genes associated with sulfur compounds and the pentose phosphate pathway. Instead, in the case of the nonself treatment, a clear response to nutrient deprivation was detected. Overall, the presented findings represent further insights into the complex functional mechanisms of self-DNA inhibition.

The integration of photovoltaic technologies within the agricultural framework, known as agrivoltaics, emerges as a promising and sustainable solution to meet the growing global demands for energy and food production. This innovative technology enables the simultaneous utilization of sunlight for both photovoltaics (PV) and photosynthesis. A key challenge in agrivoltaic research involves identifying technologies applicable to a wide range of plant species and diverse geographic regions. To address this challenge, we adopt a multi-experimental and multi-species approach to assess the viability of semi-transparent, spectrally selective thin-film silicon PV technology. Our findings demonstrate compatibility with crop production in controlled environments for both plants and algae. Notably, selective thin-film PV exhibits the potential to enhance crop yields and serves as a photo-protectant. We observe that plant and algal growth increases beneath the selective PV film when supplemented with appropriate diffuse light in the growth environment. Conversely, in situations where light intensity exceeds optimal levels for plant growth, the selective PV film provides a photo-protective effect. These results suggest potential supplementary benefits of employing this technology in regions characterized by excessive light irradiation, where it can contribute to healthy plant growth.