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Research has been carried out on the biosynthesis of gold nanoparticles using a bioreductant of Lannea coromandelica bark extract. In this study, the precursor concentration and volume of the bioreductant extract were optimized to produce more stable gold nanoparticles. The results of the biosynthesis showed that analysis using UV-Vis spectroscopy obtained an absorption spectrum in the range of 524-545 nm at different concentrations of precursor and an absorption spectrum in the range of 529.5-530.5 nm with the addition of a different volume of bioreductor extract. In the reaction, the optimum concentration was 30 ppm HAuCl4 with the addition of 4 mL of extract volume, resulting in ruby red nanoparticles with an absorption peak at a maximum wavelength of 529.5 nm. The FT-IR results show that the group that plays a role in reducing gold nanoparticles is the hydroxyl group. The XRD results confirmed that the synthesized nanoparticles were pure gold nanoparticles having a face-centered cubic structure (FCC), with the resulting crystal size of 20.9 nm. [ABSTRACT FROM AUTHOR]