1. Synthesis, characterization, and therapeutic applications of LAP/PDA composite nanomaterials in silk fibroin-based hydrogels for enhanced neuroregeneration in stroke
- Author
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Huiyuan Peng, Weihua Guo, Jinsong Wang, Weili Xia, Yu Xiong, Wenhua Xu, Weibo Dai, and Feng Zheng
- Subjects
Stroke ,LAP/PDA composite nanomaterials ,Silk fibroin hydrogels ,Human mesenchymal stem cells ,Brain-derived neurotrophic factor ,Neuroregeneration ,Engineering (General). Civil engineering (General) ,TA1-2040 - Abstract
Addressing the devastating and often irreversible effects of stroke necessitates innovative therapeutic strategies that go beyond the limitations of current treatments. This study heralds a promising interdisciplinary approach by employing LAP/PDA composite nanomaterials integrated into silk fibroin-based hydrogels. These engineered materials not only demonstrated significantly enhanced mechanical properties, but also showed excellent biocompatibility when co-cultured with human mesenchymal stem cells (hMSCs). Importantly, the application of these composite hydrogels in stroke models yielded remarkable therapeutic outcomes. The results indicated a substantial reduction in cerebral infarction volumes, improved survival rates of transplanted hMSCs, and increased neurogenesis, underlining their transformative potential in stroke management. Molecular markers such as AQP4, α-DG, and β-DG were also explored, revealing modulated astrocyte activation and cellular signaling pathways. In summary, the composite LAP/PDA nanomaterials, in conjunction with hMSCs and Brain-Derived Neurotrophic Factor (BDNF), unveil a compelling and multifaceted approach for addressing the unmet needs in stroke treatment, thereby opening new avenues in regenerative medicine and tissue engineering.
- Published
- 2024
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