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Objective To investigate the effect of Enterococcus faecalis (E. faecalis) lipoteichoic acid (LTA) on the expression of the macrophage immune checkpoint-programmed cell death protein ligand 1 (PD-L1) and explore the possible regulatory mechanisms. Methods In vitro model was established by stimulating mouse monocyte macrophage cell line (RAW264.7) with different concentrations of E. faecalis LTA. PD-L1 expression and nuclear factor kappa-B (NF-κB) p65 phosphorylation level were detected using flow cytometry, quantitative real-time polymerase chain reaction (qRT-PCR), Western blot and cellular immunofluorescence. Macrophages were pretreated with the NF-κB signaling pathway inhibitor BAY 11-7082, then the expression of PD-L1 and p65 phosphorylation level were detected by the above methods. Results LTA induced PD-L1 expression in RAW264.7 cells, and p65 phosphorylation level increased (P

Background: Carbamoyl phosphate synthetase 1 (CPS1) deficiency (OMIM 237300), an autosomal recessive rare and severe urea cycle disorder, is associated with hyperammonemia and high mortality. Methods: Herein we present 12 genetic variants identified in seven clinically well-characterized Chinese patients with CPS1 deficiency who were admitted to the Children's Medical Center of Peking University First Hospital from September 2014 to August 2023. Results: Seven patients (two male and five female patients including two sisters) experienced symptoms onset between 2 days and 13 years of age, and they were diagnosed with CPS1 deficiency between 2 months and 20 years. Peak blood ammonia levels ranged from 160 to 1,000 µmol/L. Three patients showed early-onset CPS1 deficiency, with only one surviving after treatment with sodium phenylbutyrate, N-carbamoyl-L-glutamate, and liver transplantation at 4 months, showing a favorable outcome. The remaining four patients had late-onset CPS1 deficiency, presenting with mental retardation, psychiatric symptoms, and self-selected low-protein diets. Among the 12 CPS1 variants identified in these patients, 10 were novel, with all patients exhibiting compound heterozygosity for CPS1 mutant alleles. Seven variants (c.149T > C, c.616 A > T, c.1145 C > T, c.1294G > A, c.3029 C > T, c.3503 A > T, and c.3793 C > T) resulted in single amino acid substitutions. Three frameshift variations (c.2493del, c.3067dup, and c.3241del) were identified, leading to enzyme truncation. One mutation (c.3506_3508del) caused an in-frame single amino acid deletion, while another (c.2895 + 2T > C) resulted in aberrant splicing. Conclusions: Except for two known variants, all other variants were identified as novel. No hotspot variants were observed among the patients. Our data contribute to expanding the mutation spectrum of CPS1. [ABSTRACT FROM AUTHOR]

Background: Leigh syndrome (LS) is a common mitochondrial disease caused by mutations in both mitochondrial and nuclear genes. Isoleucyl-tRNA synthetase 2 (IARS2) encodes mitochondrial isoleucine-tRNA synthetase, and variants in IARS2 have been reported to cause LS. However, the pathogenic mechanism of IARS2 variants is still unclear. Methods: Two unrelated patients, a 4-year-old boy and a 5-year-old boy diagnosed with LS, were recruited, and detailed clinical data were collected. The DNA of the patients and their parents was isolated from the peripheral blood for the identification of pathogenic variants using next-generation sequencing and Sanger sequencing. The ClustalW program, allele frequency analysis databases (gnomAD and ExAc), and pathogenicity prediction databases (Clinvar, Mutation Taster and PolyPhen2) were used to predict the conservation and pathogenicity of the variants. The gene expression level, oxygen consumption rate (OCR), respiratory chain complex activity, cellular adenosine triphosphate (ATP) production, mitochondrial membrane potential (MMP) and mitochondrial reactive oxygen species (ROS) levels were measured in patient-derived lymphocytes and IARS2-knockdown HEK293T cells to evaluate the pathogenicity of the variants. Results: We reported 2 unrelated Chinese patients manifested with LS who carried biallelic IARS2 variants (c.1_390del and c.2450G > A from a 4-year-old boy, and c.2090G > A and c.2122G > A from a 5-year-old boy), of which c.1_390del and c.2090G > A were novel. Functional studies revealed that the patient-derived lymphocytes carrying c.1_390del and c.2450G > A variants exhibited impaired mitochondrial function due to severe mitochondrial complexes I and III deficiencies, which was also found in IARS2-knockdown HEK293T cells. The compensatory experiments in vitro cell models confirmed the pathogenicity of IARS2 variants since re-expression of wild-type IARS2 rather than mutant IARS2 could rescue complexes I and III deficiency, oxygen consumption, and cellular ATP content in IARS2 knockdown cells. Conclusion: Our results not only expand the gene mutation spectrum of LS, but also reveal for the first time the pathogenic mechanism of IARS2 variants due to a combined deficiency of mitochondrial complexes I and III, which is helpful for the clinical diagnosis of IARS2 mutation-related diseases. [ABSTRACT FROM AUTHOR]

This article examines recent Sino-US film collaborations, focusing on Mulan (2020) against the backdrop of geopolitical shifts and the COVID-19 pandemic. Through in-depth interviews and a case study, it investigates the lukewarm reception of Mulan (2020) in China. The study explores how external pressures have disrupted the previously thriving partnership, leading to investment uncertainty, intensified censorship, and shifts in audience consumption towards online platforms, which have exacerbated piracy issues. It also addresses the political tensions surrounding Mulan (2020), including controversies related to Xinjiang and Hong Kong, which hindered its box-office success in Mainland China despite domestic support. A comparative analysis with Mulan (1998) assesses the evolution of feminist themes and filial piety in the 2020 adaptation. The findings indicate that while efforts were made to enhance feminist narratives and cultural motifs, the execution was superficial, treating complex cultural themes as mere embellishments rather than integral elements of the narrative. The film's portrayal of these themes within a predominantly Western framework highlights ongoing challenges in bridging cultural narratives in Sino-US film productions. The findings underscore the profound impact of geopolitics and reveal the contentious portrayal of feminism and cultural integration in Mulan (2020), emphasizing the challenges in Sino-US film collaborations. [ABSTRACT FROM AUTHOR]

Despite the prolific film production in the nascent People's Republic of China (PRC) during the 'Seventeen Years' from 1949 to 1966, Maoist cinema received limited critical analysis. While recent scholarly attention has shifted towards examining Chinese film production and exchange within the socialist bloc, a notable gap remains in the literature concerning Sino-Arab film collaboration during this period. This paper seeks to address this gap by utilizing textual analysis and archival studies to explore the practice of Sino-Arab film weeks through the lens of cultural diplomacy. Building upon the framework of the three schools of thought on cultural diplomacy, this study posits that Sino-Arab film collaboration from the 1950s to 1960s operated within the realm of people's diplomacy. This term denotes cultural diplomacy with distinctively Chinese attributes within the Cold War context. This paper also complements the third trend that focuses on the transcendental realm of the state by exploring the little-studied interactions between film screenings and reality in Sino-Arab film weeks. Under the common goal of anti-imperialism and nation-building, people's diplomacy deviates from the conventional paradigm of bipolar rivalry and embodies the spirit of internationalist solidarity among Third World countries. [ABSTRACT FROM AUTHOR]

In light of the shift from the first phase to the second phase of transnational cinema studies, this volume examines a set of practices of transnational cinema that have been taking place between China and its counterparts. In exploring transnational cinema activities in the form of film collaborations and co-productions, this volume puts the spotlight on critical trends in transnational cinemas that are not limited to Western spheres of influence. Focusing on practical applications of case studies between China, Arab countries, Denmark and the U.S. it contributes to the debates on the definition and practical applications for research on film collaboration and co-production and transnational cinema studies. [ABSTRACT FROM AUTHOR]

Background: Premature Ventricular Complexes (PVCs) are very common in clinical practice, with frequent PVCs (more than 30 beats per hour) or polymorphic PVCs significantly increasing the risk of mortality. Previous studies have shown that vagus nerve stimulation improves ventricular arrhythmias. Stimulation of the auricular distribution of the vagus nerve has proven to be a simple, safe, and effective method to activate the vagus nerve. Transcutaneous au ricular vagus nerve stimulation (taVNS) has shown promise in both clinical and experimental setting for PVCs; however, high-quality clinical studies are lacking, resulting in insufficient evidence of efficacy. Methods: The study is a prospective, randomized, parallel-controlled trial with a 1:1 ratio between the two groups. Patients will be randomized to either the treatment group (taVNS) or the control group (Sham-taVNS) with a 6-week treatment and a subsequent 12-week follow-up period. The primary outcome is the proportion of patients with a ≥ 50% reduction in the number of PVCs monitored by 24-hour Holter. Secondary outcomes include the proportion of patients with a ≥ 75% reduction in PVCs, as well as the changes in premature ventricular beats, total heartbeats, and supraventricular premature beats recorded by 24-hour Holter. Additional assessments compared score changes in PVCs-related symptoms, as well as the score change of self-rating anxiety scale (SAS), self-rating depression scale (SDS), and 36-item short form health survey (SF-36). Discussion: The TASC-V trial will help to reveal the efficacy and safety of taVNS for frequent PVCs, offering new clinical evidence for the clinical practice. Trial registration: Clinicaltrials.gov: NCT04415203 (Registration Date: May 30, 2020). [ABSTRACT FROM AUTHOR]

Natural organic matter (NOM) can impede the removal of organic micro-pollutants (OMPs) through several mechanisms, including inner filter effect, competition with the target OMP, and radical scavenging, during synchronous adsorption/photocatalysis of multi-functional composites. In this study, the fate and inhibitory mechanisms of sulfamerazine (SMZ, a model OMP) that occurred in presence of seven different NOM samples (i.e. three standard NOM surrogates, a river water sample, a carbon filter effluent and two different sand filter effluents) during the adsorption/photocatalysis by a composite of Bi2O3-TiO2 supported on powdered activated carbon (Bi2O3-TiO2/PAC, abbreviated as BTP) when exposed to visible light irradiation were revealed. The results indicated that adsorption played a greater attribution than photocatalysis on SMZ removal. The primary impediment to the adsorption and photocatalytic degradation of SMZ was attributed to the presence of terrestrial-derived, humic-like NOM fractions with high aromaticity. The adsorption efficacy of SMZ was weakened by the absorption of NOM and its degradation products onto the BTP surface. The inner filter effect, competition between NOM and SMZ, and radical scavenging were responsible for the reduced photocatalysis of SMZ. In the cases of real water matrices, the presence of inorganic anion and co-existed NOM reduced the removal of SMZ. In summary, the findings of this work offer a comprehensive comprehension of the impact of NOM fractions on photocatalysis, emphasizing the necessity to examine the interplay between NOM and background inorganic constituents in the degradation of OMP via adsorption/photocatalysis. [ABSTRACT FROM AUTHOR]

In this paper, we study a class of nonlinear stochastic Duffing oscillators with multidelay feedback. We propose an effective reduction approach with the help of center manifold theory and stochastic averaging method. Taking the initial time-delay τ as the parameter, we reduce the original system to a one-dimensional averaged Itô equation. Our analysis reveals that the original system exhibits stochastic bifurcations, including stochastic D and P bifurcations. Once we have a clear understanding of the bifurcation structure, we can use this knowledge to choose appropriate system parameters and place the system in the desired state. For instance, by adjusting the initial time-delay τ of the control system, we can stabilize the system and achieve the desired outcome. Numerical simulations also verify the theoretical results. With appropriate parameter choices, multiple time delays can destabilize the equilibrium and promote chaotic behaviors, and can also lead to more stable dynamical behavior. Remarkably, we discovered that increasing the interval of time delays and feedback numbers can enhance system stability. It may potentially serve as a novel mechanism for stabilizing stochastic systems. The study provides a solid theoretical foundation for exploring stochastic systems subject to complex time-delay feedback control, and offers a valuable framework for related fields. [ABSTRACT FROM AUTHOR]

Horizontally ordered arrangement TiO2 nanotubes with various crystalline phases were prepared by electrostatic spinning, pulsed laser deposition and annealing at different temperatures. The morphology, microstructure, crystal structure and spectral absorption of the nanotubes were characterized using scanning electron microscopy, transmission electron microscopy, X-ray diffraction and ultraviolet–visible absorption spectroscopy. Then, ordered TiO2 nanotubes in various crystal phases were deposited to gold interdigital electrodes with a spacing of 100 μm to prepare photodetectors, and their photoelectric performance were examined and compared. The findings revealed that TiO2 nanotubes that were annealed at 500 °C had a greater anatase content (93.8%), displayed a shorter response-recovery time (8.8 and 2.2 s, respectively) and a higher responsivity (70.2 μA/W) to UV light with a wavelength of 365 nm (optical power density of 4.9 mW/cm2, bias voltage of 3 V). Finally, the UV response mechanism and advantages of horizontally ordered arrangement TiO2 nanotubes with different crystalline phases are discussed. [ABSTRACT FROM AUTHOR]

Objectives: This study aimed to design a modified passive-deflation sealer injection needle and investigate its ability to improve obturation quality of single-cone technique through assessing the distribution of voids in root canals using micro-computed tomography (micro-CT). Materials and methods: Forty-eight mandibular incisors were divided into eight groups (n = 6), according to the taper of root canal preparation (0.06 or 0.04), the needle used for sealer injection (modified or commercial iRoot SP injection needle), and the obturation method (iRoot SP sealer-only or single-cone obturation). After obturation, each specimen was scanned by micro-CT. The volumetric percentage and distribution of all voids were first analyzed and compared among groups, then the open and closed voids were separately analyzed and compared among single-cone obturation groups. Results: Compared to commercial needle groups, modified needle groups showed much less voids, especially in the apical root canal part (P < 0.05). Besides, the modified needle groups produced much less open voids than commercial needle groups despite the root canal taper (P < 0.05). Conclusions: The modified passive deflation sealer injection needle could effectively improve the quality of single-cone obturation through reducing intra-canal voids, especially open voids throughout the root canal, thus might possibly be developed as an effective intra-canal sealer delivering instrument. [ABSTRACT FROM AUTHOR]

We report here a tumor‐pretargted theranostic approach for multimodality imaging‐guided synergistic cancer PDT by cascade alkaline phosphatase (ALP)‐mediated in situ self‐assembly and bioorthogonal inverse electron demand Diels–Alder (IEDDA) reaction. Using the enzymatic catalysis of ALP that continuously catalyses the dephosphorylation and self‐assembly of trans‐cyclooctene (TCO)‐bearing P‐FFGd‐TCO, a high density of fluorescent and magnetic TCO‐containing nanoparticles (FMNPs‐TCO) can be synthesized and retained on the membrane of tumor cells. They can act as 'artificial antigens' amenable to concurrently capture lately administrated tetrazine (Tz)‐decorated PS (775NP‐Tz) and carbonic anhydrase (CA) inhibitor (SA‐Tz) via the fast IEDDA reaction. This two‐step pretargeting process can further induce FMNPs‐TCO regrowth into microparticles (FMNPs‐775/SA) directly on tumor cell membranes, which is analyzed by bio‐SEM and fluorescence imaging. Thus, efficient enrichment of both SA‐Tz and 775NP‐Tz in tumors can be achieved, allowing to alleviate hypoxia by continuously inhibiting CA activity and improving PDT of tumors. Findings show that subcutaneous HeLa tumors could be completely eradicated and no tumor recurred after irradiation with an 808 nm laser (0.33 W cm−2, 10 min). This pretargeted approach may be applied to enrich other therapeutic agents in tumors to improve targeted therapy. [ABSTRACT FROM AUTHOR]

The development of catalysts for low-temperature methane combustion is crucial in addressing the greenhouse effect. An effective industrial catalyst strategy involves optimizing noble metal utilization and boosting metal–metal interaction. Here, the PdNi-H catalyst was synthesized using the self-assembly method, achieving the high dispersion and close proximity of Pd and Ni atoms compared to the counterparts prepared by the impregnation method, as confirmed by EDS mapping. The XRD and TEM results revealed Pd2+ and Ni2+ doping within the CeO2 lattice, causing distortions and forming Pd-O-Ce or Ni-O-Ce structures. These structures promoted oxygen vacancy formation in CeO2, and this was further confirmed by the Raman and XPS results. Consequently, the PdNi-H catalyst demonstrated an excellent redox ability and catalytic activity, achieving lower ignition and complete methane burning temperatures at 282 and 387 °C, respectively. The highly dispersed PdNi species played a pivotal role in activating methane for enhanced redox ability. Additionally, the narrow size distribution range contributed to more vacancies on the surface of CeO2, as confirmed by the XPS results, thereby facilitating the activation of gas phase oxygen to form oxygen species (O2−). This collaborative catalytic approach presents a promising strategy for developing efficient and stable methane combustion catalysts at low temperatures. [ABSTRACT FROM AUTHOR]