1. Characterisation, Metabolite Profile, and Antioxidant Activity of Silver Nanoparticles Synthesised Using the Algae Palmaria palmata.
- Author
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Savitri, Evika S., Auliyah, Hafidah N., and Muchtaromah, Bayyinatul
- Subjects
DULSE ,SILVER nanoparticles ,SCANNING electron microscopy ,PHYTOCHEMICALS ,ANTIOXIDANTS - Abstract
Palmaria palmata contains phytochemicals with potential antioxidant properties, such as polyphenols and mycosporins (MAAs). Phycoerythrin and phycocyanin are pigments found in the red algae species Palmaria palmata. It is believed that P. palmata derived silver nanoparticles have excellent antioxidant activity. This study aims to determine the morphological, metabolite profile and antioxidant activity of silver nanoparticle compounds synthesised using P. palmata. The green synthesis of silver nanoparticles was performed using P. palmata as a bioreductant. The morphological characteristics observed were the size and shape of the particles, which were observed using the particle size analyser (PSA) and scanning electron microscopy (SEM). Biochemical characteristics studied were secondary metabolite profiles and antioxidant activity of silver nanoparticle compounds using P. palmata. The metabolite profile was tested using High-Performance Liquid Chromatography (HPLC). The antioxidant activity was tested using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method with ascorbic acid as the positive control. The study showed that P. palmata AgNPs are spherical, with a size of 185.5 nm. P. palmata AgNP contains phenolic group metabolites of gallic acid at a retention time of 2.74 minutes with a peak height of 89.248 mAu and a relative peak area of 98.73%. The antioxidant activity of AgNP synthesised using P. palmata possesses an IC
50 value of 17.113 ± 1.584 ppm, indicating a more robust antioxidant activity compared to the extract of P. palmata, which had an IC50 value of 33.875 ± 11.238 ppm. [ABSTRACT FROM AUTHOR]- Published
- 2024
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