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Intense attosecond light sources are required for investigating ultrafast electron dynamics, such as that in molecules and atoms, etc. However, so far the achievable photon energy remains at the keV level, thereby limiting their range of applications. For instance, probing intranuclear dynamics requires photon energies in the gamma-ray regime. We propose here a scheme for generating intense attosecond gamma-ray pulses by irradiating a thin solid-density foil with intense vortex laser. In the interaction, the laser driven-foil electrons are differentially accelerated within a quarter-wave field and become highly bunched. Three-dimensional particle-in-cell simulations show that they can efficiently (with ∼ 30 % energy conversion efficiency) radiate ultrashort ( ∼ 400 as), high-flux ( > 10 10 photons per pulse), and ultrabrilliant (up to 1027 photons s−1 mm−2 mrad−2 per 0.1% BW at 1 MeV photon energy) gamma rays. With an x-ray driving laser, the scheme can even produce isolated bright sub-attosecond gamma-ray pulses that are useful for time-resolved nuclear spectroscopy and many other areas. [ABSTRACT FROM AUTHOR]

Vaccine-induced mucosal immunity and broad protective capacity against various severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants remain inadequate. Formyl peptide receptor-like 1 inhibitory protein (FLIPr), produced by Staphylococcus aureus , can bind to various Fcγ receptor subclasses. Recombinant lipidated FLIPr (rLF) was previously found to be an effective adjuvant. In this study, we developed a vaccine candidate, the recombinant Delta SARS-CoV-2 spike (rDS)-FLIPr fusion protein (rDS-F), which employs the property of FLIPr binding to various Fcγ receptors. Our study shows that rDS-F plus rLF promotes rDS capture by dendritic cells. Intranasal vaccination of mice with rDS-F plus rLF increases persistent systemic and mucosal antibody responses and CD4/CD8 T-cell responses. Importantly, antibodies induced by rDS-F plus rLF vaccination neutralize Delta, Wuhan, Alpha, Beta, and Omicron strains. Additionally, rDS-F plus rLF provides protective effects against various SARS-CoV-2 variants in hamsters by reducing inflammation and viral loads in the lung. Therefore, rDS-F plus rLF is a potential vaccine candidate to induce broad protective responses against various SARS-CoV-2 variants.IMPORTANCEMucosal immunity is vital for combating pathogens, especially in the context of respiratory diseases like COVID-19. Despite this, most approved vaccines are administered via injection, providing systemic but limited mucosal protection. Developing vaccines that stimulate both mucosal and systemic immunity to address future coronavirus mutations is a growing trend. However, eliciting strong mucosal immune responses without adjuvants remains a challenge. In our study, we have demonstrated that using a recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike-formyl peptide receptor-like 1 inhibitory protein (FLIPr) fusion protein as an antigen, in combination with recombinant lipidated FLIPr as an effective adjuvant, induced simultaneous systemic and mucosal immune responses through intranasal immunization in mice and hamster models. This approach offered protection against various SARS-CoV-2 strains, making it a promising vaccine candidate for broad protection. This finding is pivotal for future broad-spectrum vaccine development., Competing Interests: M.S.H., S.J.L., C.L.Liao, and H.W.C. are named on patent applications related to lipidated FLIPr and the use thereof in vaccines.