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Mammary epithelial cells (MEC) are responsible for milk synthesis in the bovine mammary gland during lactation, and could thereby potentially be used for in-vitro milk components production. Development of such a model, however, requires the MECs to preserve their lactogenic traits following in-vitro growth. Potential impact of growing MEC in the lab following their isolation was studied in relation to their population homogeneity and milk synthesis related gene expressions. Upon isolation, flow cytometry results showed that > 30 % of the obtained cells were positive for cytokeratin 18 (CK18), a marker for the MEC. This proportion increased to 95 % MEC after the first passage. Cell functionality was examined by comparing the expressions of milk synthesis related genes at passage 2 (P2) to passage 6 (P6) following treatment with lactogenic agents. CSN1S1 was found to be expressed significantly higher at P2 compared to P6. In addition, CSN2, CSN3 and PRLR tended to have lower expressions in P6 compared to P2. DGAT1, STAT5A, AKT1, and RPS6KB1 were expressed comparably in both passages. The expressions of LALBA and CSN1S2 were not detectable in any of the samples. Therefore, despite having high levels of CK18 positive cells at later passages, in line with other studies, further passaging affected the expressions of some of the genes related to milk synthesis. A reduced gene expression capacity in the propagated MEC may limit their potential for in-vitro milk components production.

UVCANDELS is a Hubble Space Telescope Cycle-26 Treasury Program awarded 164 orbits of primary ultraviolet (UV) F275W imaging and coordinated parallel optical F435W imaging in four CANDELS fields—GOODS-N, GOODS-S, EGS, and COSMOS—covering a total area of ∼426 arcmin ^2 . This is ∼2.7 times larger than the area covered by previous deep-field space UV data combined, reaching a depth of about 27 and 28 ABmag (5 σ in 0.”2 apertures) for F275W and F435W, respectively. Along with new photometric catalogs, we present an analysis of the rest-frame UV luminosity function (LF), relying on our UV-optimized aperture photometry method, yielding a factor of 1.5 increase over H-isophot aperture photometry in the signal-to-noise ratios of galaxies in our F275W imaging. Using well-tested photometric redshift measurements, we identify 5810 galaxies at redshifts 0.6 < z < 1, down to an absolute magnitude of M _UV = −14.2. In order to minimize the effect of uncertainties in estimating the completeness function, especially at the faint end, we restrict our analysis to sources above 30% completeness, which provides a final sample of 4726 galaxies at −21.5 < M _UV < −15.5. We performed a maximum likelihood estimate to derive the best-fit parameters of the UV LF. We report a best-fit faint-end slope of $\alpha =-{1.359}_{-0.041}^{+0.041}$ at z ∼ 0.8. Creating subsamples at z ∼ 0.7 and z ∼ 0.9, we observe a possible evolution of α with redshift. The unobscured UV luminosity density at M _UV < −10 is derived as ${\rho }_{{\rm{U}}{\rm{V}}}={1.339}_{-0.030}^{+0.027}\,(\times {10}^{26}\,{\rm{e}}{\rm{r}}{\rm{g}}\,{{\rm{s}}}^{-1}\,{{\rm{H}}{\rm{z}}}^{-1}\,{{\rm{M}}{\rm{p}}{\rm{c}}}^{-3})$ using our best-fit LF parameters. The new F275W and F435 photometric catalogs from UVCANDELS have been made publicly available on the Barbara A. Mikulski Archive for Space Telescopes.

We use the Ultraviolet Imaging of the Cosmic Assembly Near-infrared Deep Extragalactic Legacy Survey fields (UVCANDELS) to measure half-light radii in the rest-frame far-UV for ∼16,000 disk-like galaxies over 0.5 ≤ z ≤ 3. We compare these results to rest-frame optical sizes that we measure in a self-consistent way and find that the stellar mass–size relation of disk galaxies is steeper in the rest-frame UV than in the optical across our entire redshift range. We show that this is mainly driven by massive galaxies (≳10 ^10 M _⊙ ), which we find to also be among the most dusty. Our results are consistent with the literature and have commonly been interpreted as evidence of inside-out growth wherein galaxies form their central structures first. However, they could also suggest that the centers of massive galaxies are more heavily attenuated than their outskirts. We distinguish between these scenarios by modeling and selecting galaxies at z = 2 from the VELA simulation suite in a way that is consistent with UVCANDELS. We show that the effects of dust alone can account for the size differences we measure at z = 2. This indicates that, at different wavelengths, size differences and the different slopes of the stellar mass–size relation do not constitute evidence for inside-out growth.

To study the role of environment in galaxy evolution, we reconstruct the underlying density field of galaxies based on COSMOS2020 (The Farmer catalog) and provide the density catalog for a magnitude-limited ( K _s < 24.5) sample of ∼210,000 galaxies at 0.4 < z < 5 within the COSMOS field. The environmental densities are calculated using a weighted kernel density estimation approach with the choice of a von Mises–Fisher kernel, an analog of the Gaussian kernel for periodic data. Additionally, we make corrections for the edge effect and masked regions in the field. We utilize physical properties extracted by LePhare to investigate the connection between star formation activity and the environmental density of galaxies in six mass-complete subsamples at different cosmic epochs within 0.4 < z < 4. Our findings confirm a strong anticorrelation between star formation rate (SFR)/specific SFR (sSFR) and environmental density out to z ∼ 1.1. At 1.1 < z < 2, there is no significant correlation between SFR/sSFR and density. At 2 < z < 4, we observe a reversal of the SFR/sSFR–density relation such that both SFR and sSFR increase by a factor of ∼10 with increasing density contrast, δ , from −0.4 to 5. This observed reversal at higher redshifts supports the scenario where an increased availability of gas supply, along with tidal interactions and a generally higher star formation efficiency in dense environments, could potentially enhance star formation activity in galaxies located in rich environments at z > 2.