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Abstract Background Posterior fossa malformation (PFM) is a relatively uncommon prenatal brain malformation. Genetic diagnostic approaches, including chromosome karyotyping, copy number variant (CNV) testing, and whole-exome sequencing (WES), have been applied in several cases of fetal structural malformations. However, the clinical value of appropriate genetic diagnostic approaches for different types of PFMs has not been confirmed. Therefore, in this study, we aimed to analyze the value of different combined genetic diagnostic approaches for various types of fetal PFMs. Methods This retrospective study was conducted at Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital. Fifty-one pregnant women diagnosed with fetal PFMs who underwent genetic testing in our hospital from January 1, 2017 to December 31, 2022 were enrolled; women with an isolated enlarged cisterna magna were excluded. All participants were categorized into two groups according to the presence of other abnormalities: isolated and non-isolated PFMs groups. Different combined approaches, including karyotype analysis, CNV testing, and trio-based WES, were used for genetic analysis. The detection rates of karyotype analysis, CNV testing, and WES were measured in the isolated and non-isolated groups. Results In isolated PFMs, pathogenic/likely pathogenic (P/LP) CNVs were detected in four cases (36.36%, 4/11), whereas G-banding karyotyping and WES showed negative results. In non-isolated PFMs, a sequential genetic approach showed a detection rate of 47.5% (19/40); karyotyping revealed aneuploidies in five cases (16.67%, 5/30), CNV testing showed P/LP CNVs in five cases (16.13%, 5/31), and WES identified P/LP variants (in genes CEP20, TMEM67, OFD1, PTPN11, ARID1A, and SMARCA4) in nine cases (40.91%, 9/22). WES showed a detection rate of 83.33% (5/6) in fetuses with Joubert syndrome. Only six patients (five with Blake’s pouch cyst and one with unilateral cerebellar hemisphere dysplasia) survived. Conclusions We recommend CNV testing for fetuses with isolated PFMs. A sequential genetic approach (karyotyping, CNV testing, and WES) may be beneficial in fetuses with non-isolated PFMs. Particularly, we recommend WES as the first-line genetic diagnostic tool for Joubert syndrome.

The cotton-picking robot needs to locate the target object in space in the process of picking in the field and other outdoor strong light complex environments. The difficulty of this process was binocular matching. Therefore, this paper proposes an accurate and fast binocular matching method. This method used the deep learning model to obtain the position and shape of the target object, and then used the matching equation proposed in this paper to match the target object. Matching precision of this method for cotton matching was much higher than that of similar algorithms. It was 54.11, 45.37, 6.15, and 12.21% higher than block matching (BM), semi global block matching (SGBM), pyramid stereo matching network (PSMNet), and geometry and context for deep stereo regression (GC-net) respectively, and its speed was also the fastest. Using this new matching method, the cotton was matched and located in space. Experimental results show the effectiveness and feasibility of the algorithm.

K403 nickel-base superalloy is widely used in the manufacture of aero-engine turbine blades because of its excellent properties at room temperature and high temperature. In order to solve the problem of turbine blade crack defects caused by long-term service in complex working conditions，in this work，two different processes of （tungsten inert gas，TIG） welding and laser cladding were used to repair the blade cracks，and the microstructure and properties of the repaired region were studied. The influence of TIG welding and laser cladding repairing on microstructure，mechanical properties and failure behavior was analyzed. The results show that the microcracks tend to occur near the repair interface using the TIG welding repairing process，which are mainly caused by carbides and low melting point eutectic structure. The grain and structure of the repaired area by laser cladding repair technology are more uniform，and the microcrack defects can be easier to control. The comprehensive mechanical properties of the samples repaired by laser cladding are obviously higher than those repaired by TIG welding repairing process，and the samples repaired by laser cladding have better process stability. The tensile strengths of the samples using the laser cladding repair process and the TIG welding repair process at room temperature have reached 87.44% and 69.22% of the strength of K403 base material，respectively. According to the failure analysis results，the tensile fracture at room temperature in the repaired region presents mixed fracture characteristics，and the tensile fracture at high temperature presents intergranular fracture characteristics. Microcracks in the repaired area，local liquid phase deficiency defects and carbide structure are the main reasons of failure. The laser cladding technology has the advantages of heat source concentration and smaller heat affected zone，which can effectively restrain the defects and refine the microstructure. Therefore，the laser cladding repair process is used to repair the edge plate crack damage generated during the blade test run. After fluorescence and kerosene-chalk detection，the repairing process meets the relevant reuse requirements.

Selective laser melting（SLM）can achieve nearly net-shape complex parts of GH3536 alloy，and its high temperature mechanical properties are important indicators for safe service. The effect of heat treatment on microstructure and high temperature tensile properties of SLM GH3536 alloy were studied. The heat treatment at 1225 ℃ for 1 h was carried out to explore the regulation mechanism of microstructure and properties. The results show that heat treatment can effectively eliminate the cellular subgrain structure inside the grains，which significantly enhances the dislocation slip ability. The tensile elongations at room temperature，650 ℃ and 815 ℃ are increased by 75%，92% and 683%，respectively. In addition，the decrease of the aspect ratio of the columnar grains significantly reduces the anisotropy in the heat-treated sample. The fracture analysis shows that the fracture mode of the heat-treated sample changes from intergranular fracture to mixed fracture with the increase of tensile environment temperature.

With the development of UAV technology, UAVs have the advantages of convenience, speed, and small size. However, when faced with a complex task environment, some traditional artificial intelligence algorithms will significantly increase computation time, and the algorithms that perform better in time performance often fail to adapt to the type and number of random tasks to be assigned. In this paper, we combine the Hungarian algorithm with improving the LightGBM model, simulate the implementation in various task scenarios, and verify that the improved LightGBM model completes the intelligent assignment of multiple UAVs without increasing time cost compared to the classical algorithm through comparative analysis. It is shown that the improved LightGBM model has an average time of 26.39s for 10 runs, which is higher than 16.91s for NSGA-II and 14.73s for PESA, but lower than 46.92s for SPEA2 and 50.01s for SPGA2. For a non-real-time assignment system, a better mission intelligence assignment at a certain time cost scheme is more in line with the system’s performance requirements. Therefore, the improved LightGBM model in this paper can obtain better solutions than the other four algorithms in solving the multi-objective multi-UAV task intelligence assignment problem, and the solution set coverage is larger than several other algorithms. This indicates that the algorithm studied in this paper is feasible in solving the multi-objective multi-UAV task intelligence assignment problem, and it also has some reference significance for solving the multi-objective problem and improving the coverage of the multi-objective solution set.

With the rapid development of society, people’s awareness of environmental protection continues to strengthen, countries also pay more and more attention to environmental protection, whether from the national policy or from the green development of enterprises in the future, the improvement of power plant operation efficiency is imperative. Among them, improving the operation efficiency of thermal power plants is the top priority, and it is also an important link of national economic transformation. The design of electrical part is an important part of improving the operation efficiency of thermal power plants. 2x300MW thermal power plant is designed, including the power plant electrical main wiring design, plant power design, the main circuit of the power plant short-circuit calculation, and it choose the electrical stability and thermal stability of the better electrical equipment. The short circuit calculation of the main circuit of the power plant is carried out and preliminary planning of the secondary circuit scheme is made. A power plant with higher operating efficiency is designed. While improving the operation and economic efficiency of the power plant, it also ensures the reliability, stability and economy of the power plant.

As a kind of proteolytic enzyme extracted from earthworms, lumbrokinase has been used as an antithrombotic drug clinically. Nevertheless, its potential in anti-cancer, especially in anti-non-small cell lung cancer (NSCLC), as a single form of treatment or in combination with other therapies, is still poorly understood. In this study, we explored the anti-tumor role and the responsive molecular mechanisms of lumbrokinase in suppressing tumor angiogenesis and chemoresistance development in NSCLC and its clinical potential in combination with bevacizumab and chemotherapeutics. Lumbrokinase was found to inhibit cell proliferation in a concentration-dependent manner and caused metastasis suppression and apoptosis induction to varying degrees in NSCLC cells. Lumbrokinase enhanced the anti-angiogenesis efficiency of bevacizumab by down-regulating BPTF expression, decreasing its anchoring at the VEGF promoter region and subsequent VEGF expression and secretion. Furthermore, lumbrokinase treatment reduced IC50 values of chemotherapeutics and improved their cytotoxicity in parental and chemo-resistant NSCLC cells via inactivating the NF-κB pathway, inhibiting the expression of COX-2 and subsequent secretion of PGE2. LPS-induced NF-κB activation reversed its inhibition on NSCLC cell proliferation and its synergy with chemotherapeutic cytotoxicity, while COX-2 inhibitor celecoxib treatment boosted such effects. Lumbrokinase combined with bevacizumab, paclitaxel, or vincristine inhibited the xenograft growth of NSCLC cells in mice more significantly than a single treatment. In conclusion, lumbrokinase inhibited NSCLC survival and sensitized NSCLC cells to bevacizumab or chemotherapeutics treatment by targeted down-regulation of BPTF/VEGF signaling and inactivation of NF-κB/COX-2 signaling, respectively. The combinational applications of lumbrokinase with bevacizumab or chemotherapeutics are expected to be developed as promising candidate therapeutic strategies to improve the efficacy of the original monotherapy in anti-NSCLC. [ABSTRACT FROM AUTHOR]

Oncolytic virotherapy is expected to provide a new treatment strategy for cancer. Aphrocallistes vastus lectin (AVL) is a Ca2+-dependent lectin receptor containing the conserved domain of C-type lectin and the hydrophobic N-terminal region, which can bind to the bird's nest glycoprotein and D-galactose. Our previous studies suggested that the oncolytic vaccinia virus (oncoVV) armed with the AVL gene exerted remarkable replication and antitumor effects in vitro and in vivo. In this study, we found that oncoVV-AVL may reprogram the metabolism of hepatocellular carcinoma cells to promote ROS, and elevated ROS subsequently promoted viral replication and induced apoptosis. This study will provide a new theoretical basis for the application of oncoVV-AVL in liver cancer. [ABSTRACT FROM AUTHOR]

The preparation of a single crystal superalloy surface overlay welding coating to improve its high-temperature mechanical properties is of great significance for prolonging the service life of blades. This work selected two types of welding wire alloys, CoCrMo and CoCrW, to prepare coatings on the surface of a single crystal superalloy. A comparative study was conducted on their mechanical properties, such as tension, compression, fatigue, durability, and wear at a high temperature of 900 ℃, aiming to reveal the high-temperature mechanical properties of the two types of welding coatings. Results showed that the average high-temperature tensile strength of the CoCrMo welded specimen was smaller than that of the CoCrW welded specimen; the average high-temperature duration of CoCrMo welded specimens at 150 MPa was lower than the average duration of CoCrW welded specimens; the high-temperature fatigue life of CoCrMo welded specimens at 220 MPa was 7.186 × 105; and the average high-temperature wear rate of CoCrMo sample was 3.64 × 10−6 mm3·N−1·m−1. The CoCrW alloy was more wear resistant than CoCrMo. The hardness of CoCrMo welded joints gradually increased from the substrate to the heat-affected zone and then to the fusion zone, and was much higher in the fusion zone than in the CoCrW alloy. [ABSTRACT FROM AUTHOR]