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4. Lowering systolic blood pressure to less than 120 mm Hg versus less than 140 mm Hg in patients with high cardiovascular risk with and without diabetes or previous stroke: an open-label, blinded-outcome, randomised trial

Author

Ai, Xinyue, An, Chun, An, Yuhong, Bai, Shiru, Bai, Xueke, Bi, Jingao, Bin, Xiaoling, Bu, Miaomiao, Bu, Peili, Bu, Wei, Cai, Lvping, Cai, Nana, Cai, Shuhui, Cai, Ting, Cai, Wenjing, Cao, Bin, Cao, Bingbing, Cao, Huaping, Cao, Libo, Cao, Xiancun, Chai, Hui, Chai, Yonggui, Chai, Zhiyong, Chang, Chunduo, Chang, Jianbao, Chang, Shuyue, Chang, Yunling, Chao, Huanhuan, Che, Hang, Che, Qianqiu, Chen, Danlin, Chen, Dongsheng, Chen, Faxiu, Chen, Guang, Chen, Hairong, Chen, Hao, Chen, Huahua, Chen, Huijun, Chen, Jiafu, Chen, Jian, Chen, Jiasen, Chen, Jing, Chen, Jinzi, Chen, Junrong, Chen, lichun, Chen, Lijuan, Chen, Liyuan, Chen, Qun, Chen, Run, Chen, Shaoxing, Chen, Song, Chen, Tieshuang, Chen, Xianghong, Chen, Xiaowu, Chen, Xudong, Chen, Xue, Chen, Xunchun, Chen, Yao, Chen, Yongli, Chen, Yuanyue, Chen, Yuhong, Chen, Yuyi, Chen, Zhangying, Chen, Zhidong, Chen, Zuyi, Cheng, Caiming, Cheng, Jianbin, Cheng, Xiaoxia, Chu, Junjie, Cui, Ruifeng, Cui, Xiaolin, Cui, Xuechen, Cui, Yang, Cui, Zhonghua, Dai, Wanhong, Dai, Xing, Ding, Chunxia, Ding, Huihong, Ding, Qiuhong, Ding, Yaozong, Ding, Yingjie, Dong, Jiajia, Dong, Lei, Dong, Qi, Dong, Yumei, Du, Bing, Du, Hong, Du, Jie, Du, Laijing, Du, Meiling, Du, Qiong, Du, Tianmin, Du, Xue, Duan, Ru, Duan, Xiaojing, Duan, Xiaoting, Fan, Dandan, Fan, Xiaohong, Fan, Xin, Fang, Fang, Fang, Jing, Fang, Xibo, Fang, Yang, Feng, Erke, Feng, Hejin, Feng, Ling, Feng, Rui, Feng, Zhaohui, Fu, Hongmei, Fu, Qiuai, Gao, Haofei, Gao, Li, Gao, Lina, Gao, Liwei, Gao, Lu, Gao, Min, Gao, Qian, Gao, Yan, Gao, Yuan, Ge, Jinzhuo, Geng, Hongxu, Geng, Hui, Geng, Leijun, Geng, Lianqing, Gou, Hongyan, Gu, Qin, Guan, Lili, Guan, Shuo, Guan, Wenchi, Guan, Zheng, Guang, Bin, Guo, Anran, Guo, Changhong, Guo, Gaofeng, Guo, Lizhi, Guo, Qing, Guo, Qiue, Guo, Ying, Guo, Zhihua, Han, Aihong, Han, Meihong, Han, Suhui, Han, Xinru, Han, Yajun, Hao, Feng, Hao, Jingmin, Hao, Shiguo, He, Chuanhui, He, Dejian, He, Mengyuan, He, Miaomiao, He, Shaojuan, He, Wenkai, He, Xiaoyu, He, Yuxiang, Hong, Jige, Hou, Chuanxing, Hou, Jing, Hu, Danli, Hu, Jian, Hu, Jun, Hu, Lingai, Hu, Mengying, Hu, Zhiyuan, Huang, Anhui, Huang, Chunxia, Huang, Haolin, Huang, Jianlan, Huang, Sha, Huang, Siqi, Huang, Weijun, Huang, Wenxiu, Huang, Xinghe, Huang, Xinsheng, Huang, Xinxin, Hui, Jiliang, Hui, Lijun, Hui, Zhongsheng, Huo, Fangjie, Ji, Runqing, Jia, Guojiong, Jia, Hao, Jia, Jingjing, Jia, Jingmei, Jia, Xiaoling, Jiang, Hua, Jiang, Jingcheng, Jiang, Qian, Jiang, Xianyan, Jiang, Xiaoyuan, Jiang, Yanxiang, Jiao, Yunhong, Jie, Liying, Jin, Binbin, Jin, Lingjiao, Jin, Renshu, Jin, Rong, Jin, Xiang, Jin, Xianping, Jin, Yongfan, Jin, Zepu, Jin, Zhenan, Jing, Chengrong, Jing, Jiajie, Jing, Ruiling, Kang, Liping, Kang, Yu, Kong, Jianqiong, Kou, Shijie, Kou, Xianli, Kulaxihan, Lai, Jijia, Lei, Lubi, Li, Baoxiang, Li, Bin, Li, Bing, Li, Chaohui, Li, Cheng, Li, Chunmei, Li, Chunyan, Li, Daqing, Li, Deen, Li, Di, Li, Feng, Li, Guanyi, Li, Haiyang, Li, Hongwei, Li, Jia, Li, Jialin, Li, Jianan, Li, Jianguang, Li, Jiaying, Li, Jing, Li, Jinmei, Li, Lala, Li, Li, Li, Lijun, Li, Liping, Li, Lize, Li, Mingju, Li, Minglan, Li, Mingyan, Li, Na, Li, Nan, Li, Nana, Li, Qiang, Li, Qianru, Li, Rui, Li, Ruihong, Li, Shanshan, Li, Shilin, Li, Si, Li, Suwen, Li, Tongshe, Li, Tongying, Li, Wanke, Li, Wei, Li, Wenbo, Li, Wenjuan, Li, Xi, Li, Xiangxia, Li, Xiao, Li, Xiaohui, Li, Xingyan, Li, Xiujuan, Li, Yan, Li, Yanfang, Li, Yang, Li, Yanxia, Li, Yaona, Li, Yichong, Li, Ying, Li, Yuqing, Li, Zheng, Li, Zhengye, Liang, Chuanliang, Liang, Jihua, Liang, Jin, Liang, Ke, Liang, Linju, Liang, Tingchen, Liang, Xia, Liang, Xianfeng, Liang, Yanli, Liang, Zhenye, Lie, Zhenbang, Lin, Qingfei, Lin, Ruifang, Lin, Xiao, Lin, Zhiqiang, Liu, Aijun, Liu, Chao, Liu, Chunxia, Liu, Cong, Liu, Fang, Liu, Guaiyan, Liu, Hongjun, Liu, Jiamin, Liu, Jiangling, Liu, Jianqi, Liu, Jieyun, Liu, Jihong, Liu, Jing, Liu, Jinsha, Liu, Juan, Liu, Junfang, Liu, Liming, Liu, Lin, Liu, Ling, Liu, Lu, Liu, Qiang, Liu, Qiaoling, Liu, Qiaoxia, Liu, Qiuxia, Liu, Shaobo, Liu, Xiaobao, Liu, Xiaocheng, Liu, Xiaoyuan, Liu, Xinbo, Liu, Xu, Liu, Yang, Liu, Yanhu, Liu, Yanming, Liu, Yaqin, Liu, Yong, Liu, Zhihong, Long, Jing, Lu, Futang, Lu, Huamei, Lu, Jiapeng, Lu, Junhong, Lu, Weibin, Lu, Yanrong, Lu, Yuchun, Luan, Tianwei, Luo, Qingwei, Luo, Qun, Luo, Tian, Luo, Xia, Luo, Yongmei, Lv, Jing, Lv, Jinhai, Lv, Lei, Lv, Lili, Lv, Meng, Ma, Aiqing, Ma, Huaimin, Ma, Huihuang, Ma, Jie, Ma, Jinbao, Ma, Li, Ma, Lingzhen, Ma, Nan, Ma, Qiaojuan, Ma, Shumei, Ma, Tengfei, Ma, Xiange, Ma, Xiaowen, Ma, Yuehua, Mai, Lanxian, Mei, Xiao, Meng, Gen, Miao, Ruichao, Miao, Xue, Miao, Xuyan, Min, Tingting, Mo, Shubing, Morigentu, Nan, Tingyan, Ni, Jinyang, Ni, Shuguo, Nie, Yu, Ning, Benxing, Ning, Xiaowei, Niu, Manman, Niu, Qingying, Niu, Wentang, Niu, Xiaoxia, Ou, Fang, Pan, Biyun, Pan, Chengjie, Pan, Congming, Pan, Jieli, Pan, Xiaowen, Pan, Ziying, Pei, Guangzhong, Pei, Lingyu, Pei, Min, Pei, Yanzhen, Peng, Yinyu, Peng, Yuming, Pu, Zhaokun, Qi, Fengjun, Qi, Liwei, Qi, Meiqiong, Qi, Yan, Qian, Jun, Qin, Lei, Qin, Zhonghua, Qing, Lan, Qiu, Lixia, Qiu, Weiyu, Qiu, Xiaoling, Qu, Yueli, Quan, Minghua, Ren, Dingping, Ren, Hong, Ren, Lingzhi, Ren, Tingting, Ren, Wei, Ren, Yihui, Rong, Yufang, Ruan, Jiahui, Shang, Peiqin, Shao, Minyan, Shao, Xuefeng, Shao, Yuling, Shen, Junrong, Shen, Rui, Sheng, Lin, Shi, Jiangjie, Shi, Xun, Shi, Yanhong, Shi, Yeju, Shi, Yujiao, Shu, Bo, Song, Bingchun, Song, Dan, Song, Jinhui, Song, Jinwang, Song, Jinxian, Song, Wei, Song, Xiaoping, Song, Yawen, Su, He, Su, Qinfeng, Su, Shuhong, Su, Xiaozhou, Sun, Chengxiang, Sun, Fangfang, Sun, Gongping, Sun, Jiangnan, Sun, Mengmeng, Sun, Rongrong, Sun, Shuting, Sun, Songtao, Sun, Ying, Sun, Yongmiao, Sun, Yunhong, Sun, Zhiqiang, Suo, Mengying, Tan, Binghu, Tang, Chunyan, Tang, Zhongli, Tao, Yu, Tian, Changming, Tian, Hongmei, Tian, Jian, Tian, Xiaomin, Wan, Huaibin, Wan, Qin, Wan, Rongjun, Wang, Bobin, Wang, Chao, Wang, Chaoqun, Wang, Chengliang, Wang, Di, Wang, Enfang, Wang, Feng, Wang, Gang, Wang, Guangqiang, Wang, Guixiang, Wang, Haifeng, Wang, Haijun, Wang, Haiyang, Wang, Jianfang, Wang, Jianfeng, Wang, Jing, Wang, Junping, Wang, Junying, Wang, Kang, Wang, Lei, Wang, Lin, Wang, Lize, Wang, Meng, Wang, Pan, Wang, Qi, Wang, Qiong, Wang, Qiuli, Wang, Qiuxue, Wang, Ran, Wang, Shaojin, Wang, Shuai, Wang, Tao, Wang, Tiantian, Wang, Tinghui, Wang, Tongyan, Wang, Wanhong, Wang, Wenjuan, Wang, Wenyan, Wang, Wenying, Wang, Wenzhuan, Wang, Xiaofei, Wang, Xiaoyan, Wang, Xitong, Wang, Xu, Wang, Yan, Wang, Yanfang, Wang, Yang, Wang, Yanping, Wang, Yanying, Wang, Yaoxin, Wang, Yingli, Wang, Yiting, Wang, Yue, Wang, Yumei, Wang, Yuzhuo, Wang, Zhenhua, Wang, Zhifang, Wang, Zhimin, Wei, Chunli, Wei, Lixia, Wei, Pei, Wei, Shuying, Wei, Xiqing, Wen, Hong, Wen, Yun, Wu, Chaoqun, Wu, Hairong, Wu, Lihua, Wu, Lingxiang, Wu, Qi, Wu, Shaorong, Wu, Wenting, Wu, Xueyi, Wu, Yongshuan, Wu, Zhihao, Wu, Zhuying, Wu, Zongyin, Wuhanbilige, Xia, Jun, Xia, Yang, Xiang, Jing, Xiao, Heliu, Xiao, Yaying, Xie, Meiling, Xie, Yinyan, Xin, Huiling, Xing, Jing, Xiu, Guoquan, Xu, Baohua, Xu, Chuangze, Xu, En, Xu, Jian, Xu, Shuli, Xu, Wei, Xu, Wen, Xue, Na, Xue, Tingting, Xue, Wei, Yan, Haiyan, Yan, Xiaofang, Yan, Yanqing, Yang, Bo, Yang, Hui, Yang, Huiyu, Yang, Jinhua, Yang, Kun, Yang, Man, Yang, Mengya, Yang, Ning, Yang, Ping, Yang, Xiajiao, Yang, Xiaomo, Yang, Xin, Yang, Xiujuan, Yang, Xuemei, Yang, Xuming, Yang, Yan, Yang, Yanhua, Yang, Yi, Yang, Yuanyuan, Yang, Zhimei, Yang, Zhiming, Yao, Hui, Yao, Lu, Ye, Jinling, Ye, Wenhua, Yi, Mingjiao, Yi, Shaowei, Yi, Wenyi, Yi, Zhimin, Yin, Guangxia, Yin, Guoyuan, Yu, Guibin, Yu, Hairong, Yu, Huaitao, Yu, Lijie, Yu, Lijun, Yu, Nana, Yu, Qin, Yu, Xinli, Yu, Yi, Yuan, Biao, Zeng, Chunmei, Zhai, Na, Zhai, Xiaojuan, Zhan, Hongju, Zhang, Aizhen, Zhang, Baohua, Zhang, Bin, Zhang, Caizhu, Zhang, Chaoying, Zhang, Chengbo, Zhang, Chunlai, Zhang, Churuo, Zhang, Fan, Zhang, Feiqin, Zhang, Ge, Zhang, Haibo, Zhang, Hailin, Zhang, Hanxue, Zhang, Huaixing, Zhang, Hui, Zhang, Huijuan, Zhang, Jinguo, Zhang, Jingyu, Zhang, Jinyun, Zhang, Jisheng, Zhang, Jun, Zhang, Lei, Zhang, Li, Zhang, Liang, Zhang, Lifeng, Zhang, Lina, Zhang, Liping, Zhang, Min, Zhang, Ping, Zhang, Qiang, Zhang, Rufang, Zhang, Ruifen, Zhang, Shengde, Zhang, Siqi, Zhang, Sufang, Zhang, Tingting, Zhang, Wanyue, Zhang, Weiliang, Zhang, Xiaohan, Zhang, Xiaohong, Zhang, Xiaojuan, Zhang, Xin, Zhang, Xue, Zhang, Xuewei, Zhang, Yachen, Zhang, Yang, Zhang, Yanyan, Zhang, Yaojie, Zhang, Yingyu, Zhang, Yuan, Zhang, Yun, Zhang, Yunfeng, Zhang, Zaozhang, Zhang, Zhichao, Zhao, Baihui, Zhao, Dan, Zhao, Fuxian, Zhao, Guizeng, Zhao, Haijie, Zhao, Honglei, Zhao, Huizhen, Zhao, Jindong, Zhao, Juan, Zhao, Liming, Zhao, Ling, Zhao, Lingshan, Zhao, Qingxia, Zhao, Qiuping, Zhao, Wanchen, Zhao, Wangxiu, Zhao, Weiyi, Zhao, Xiaodi, Zhao, Xiaojing, Zhao, Xiaoli, Zhao, Xiaoyan, Zhao, Xiling, Zhao, Yannan, Zhao, Yiyuan, Zheng, Shuzhen, Zheng, Xin, Zhi, Lixia, Zhong, Hui, Zhong, Qing, Zhong, Xin, Zhong, Yunzhi, Zhou, Jianfeng, Zhou, Jihu, Zhou, Ke, Zhou, Liangliang, Zhou, Ling, Zhou, Na, Zhou, Shengcheng, Zhou, Suyun, Zhou, Tao, Zhou, Wanren, Zhou, Weifeng, Zhou, Weijuan, Zhou, Xiaohong, Zhou, Yunke, Zhou, Yuquan, Zhou, Zhaohai, Zhou, Zhiming, Zhu, Bingpo, Zhu, Jifa, Zhu, Jing, Zhu, Mengnan, Zhu, Youcun, Zong, Dafei, Zuo, Hongbo, and Zuo, Zhaokai

Published
2024
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20. Ergothioneine: new functional factor in fermented foods.

Author

Xiong, Kexin, Xue, Siyu, Guo, Hui, Dai, Yiwei, Ji, Chaofan, Dong, Liang, and Zhang, Sufang

Abstract

Ergothioneine (EGT) is a high-value natural sulfur-containing amino acid and has been shown to possess extremely potent antioxidant and cytoprotective activities. At present, EGT has been widely used in food, functional food, cosmetics, medicine, and other industries, but its low yield is still an urgent problem to overcome. This review briefly introduced the biological activities and functions of EGT, and expounded its specific applications in food, functional food, cosmetic, and medical industries, introduced and compared the main production methods of EGT and respective biosynthetic pathways in different microorganisms. Furthermore, the use of genetic and metabolic engineering methods to improve EGT production was discussed. In addition, the incorporation of some food-derived EGT-producing strains into fermentation process will allow the EGT to act as a new functional factor in the fermented foods. [ABSTRACT FROM AUTHOR]

Published
2024
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21. Functional Characterization of Abdominal-A in the Pine Caterpillar Moth, Dendrolimus punctatus.

Author

Liu, Huihui, Chen, Lin, Ma, Qinghua, Wang, Xinghong, Zhang, Sufang, and Zhou, Xuguo

Subjects
HOMEOBOX genes, REGULATOR genes, INSECT development, INSECT genes, EMBRYOLOGY
Abstract

Hox genes, specifically the bithorax complex (ubx, abdominal-a, and abdominal-b), play a crucial role in specifying posterior abdominal development and serve as key regulators of germline gene development in insects. However, the function of the bithorax complex in the pine caterpillar moth, Dendrolimus punctatus, a major pine tree defoliator in China, remains largely unknown. Specifically, Abdominal-A (Abd-a) controls regional variation in abdominal segmentation in model insects such as Drosophila and Tribolium; however, its role in D. Punctatus remains unexplored. In this study, CRISPR/Cas9 was used to functionally characterize Abd-a in D. punctatus. Two target sites were selected, and the genotypes and phenotypes of the G0 and G1 generations were evaluated. Our findings indicate that knocking out Abd-a led to an abnormality in the posterior segments A2–A7, as well as the loss of appendages, mainly prolegs, and affected the thoracic T3 segmentation as well as wing development. Moreover, mutation in Abd-a also impacted anal and reproductive development. Taken together, these results demonstrate that DpAbd-a is essential for embryonic and reproductive development in D. punctatus and could be a promising target for genetic control of this devastating conifer defoliator. [ABSTRACT FROM AUTHOR]

Published
2024
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26. Lowering systolic blood pressure to less than 120 mm Hg versus less than 140 mm Hg in patients with high cardiovascular risk with and without diabetes or previous stroke: an open-label, blinded-outcome, randomised trial

Author

Liu, Jiamin, Li, Yan, Ge, Jinzhuo, Yan, Xiaofang, Zhang, Haibo, Zheng, Xin, Lu, Jiapeng, Li, Xi, Gao, Yan, Lei, Lubi, Liu, Jing, Li, Jing, Ai, Xinyue, An, Chun, An, Yuhong, Bai, Shiru, Bai, Xueke, Bi, Jingao, Bin, Xiaoling, Bu, Miaomiao, Bu, Peili, Bu, Wei, Cai, Lvping, Cai, Nana, Cai, Shuhui, Cai, Ting, Cai, Wenjing, Cao, Bin, Cao, Bingbing, Cao, Huaping, Cao, Libo, Cao, Xiancun, Chai, Hui, Chai, Yonggui, Chai, Zhiyong, Chang, Chunduo, Chang, Jianbao, Chang, Shuyue, Chang, Yunling, Chao, Huanhuan, Che, Hang, Che, Qianqiu, Chen, Danlin, Chen, Dongsheng, Chen, Faxiu, Chen, Guang, Chen, Hairong, Chen, Hao, Chen, Huahua, Chen, Huijun, Chen, Jiafu, Chen, Jian, Chen, Jian, Chen, Jiasen, Chen, Jing, Chen, Jinzi, Chen, Junrong, Chen, lichun, Chen, Lijuan, Chen, Liyuan, Chen, Qun, Chen, Run, Chen, Shaoxing, Chen, Song, Chen, Tieshuang, Chen, Xianghong, Chen, Xiaowu, Chen, Xudong, Chen, Xue, Chen, Xunchun, Chen, Yao, Chen, Yongli, Chen, Yuanyue, Chen, Yuhong, Chen, Yuyi, Chen, Zhangying, Chen, Zhidong, Chen, Zuyi, Cheng, Caiming, Cheng, Jianbin, Cheng, Xiaoxia, Chu, Junjie, Cui, Ruifeng, Cui, Xiaolin, Cui, Xuechen, Cui, Yang, Cui, Zhonghua, Dai, Wanhong, Dai, Xing, Ding, Chunxia, Ding, Huihong, Ding, Qiuhong, Ding, Yaozong, Ding, Yingjie, Dong, Jiajia, Dong, Lei, Dong, Qi, Dong, Yumei, Du, Bing, Du, Hong, Du, Jie, Du, Laijing, Du, Meiling, Du, Qiong, Du, Tianmin, Du, Xue, Duan, Ru, Duan, Xiaojing, Duan, Xiaoting, Fan, Dandan, Fan, Xiaohong, Fan, Xin, Fang, Fang, Fang, Jing, Fang, Xibo, Fang, Yang, Feng, Erke, Feng, Hejin, Feng, Ling, Feng, Rui, Feng, Zhaohui, Fu, Hongmei, Fu, Qiuai, Gao, Haofei, Gao, Li, Gao, Lina, Gao, Liwei, Gao, Lu, Gao, Min, Gao, Min, Gao, Qian, Gao, Yan, Gao, Yuan, Ge, Jinzhuo, Geng, Hongxu, Geng, Hui, Geng, Leijun, Geng, Lianqing, Gou, Hongyan, Gu, Qin, Guan, Lili, Guan, Shuo, Guan, Wenchi, Guan, Zheng, Guang, Bin, Guo, Anran, Guo, Changhong, Guo, Gaofeng, Guo, Lizhi, Guo, Qing, Guo, Qiue, Guo, Ying, Guo, Zhihua, Han, Aihong, Han, Meihong, Han, Suhui, Han, Xinru, Han, Yajun, Hao, Feng, Hao, Jingmin, Hao, Shiguo, He, Chuanhui, He, Dejian, He, Mengyuan, He, Miaomiao, He, Shaojuan, He, Wenkai, He, Xiaoyu, He, Yuxiang, Hong, Jige, Hou, Chuanxing, Hou, Jing, Hu, Danli, Hu, Jian, Hu, Jun, Hu, Lingai, Hu, Mengying, Hu, Zhiyuan, Huang, Anhui, Huang, Chunxia, Huang, Haolin, Huang, Jianlan, Huang, Sha, Huang, Siqi, Huang, Weijun, Huang, Wenxiu, Huang, Xinghe, Huang, Xinsheng, Huang, Xinxin, Hui, Jiliang, Hui, Lijun, Hui, Zhongsheng, Huo, Fangjie, Ji, Runqing, Ji, Runqing, Jia, Guojiong, Jia, Hao, Jia, Jingjing, Jia, Jingmei, Jia, Xiaoling, Jiang, Hua, Jiang, Jingcheng, Jiang, Qian, Jiang, Xianyan, Jiang, Xiaoyuan, Jiang, Yanxiang, Jiao, Yunhong, Jie, Liying, Jin, Binbin, Jin, Lingjiao, Jin, Renshu, Jin, Rong, Jin, Xiang, Jin, Xianping, Jin, Yongfan, Jin, Zepu, Jin, Zhenan, Jing, Chengrong, Jing, Jiajie, Jing, Ruiling, Kang, Liping, Kang, Yu, Kong, Jianqiong, Kou, Shijie, Kou, Xianli, Kulaxihan, Lai, Jijia, Lei, Lubi, Li, Baoxiang, Li, Bin, Li, Bing, Li, Chaohui, Li, Cheng, Li, Chunmei, Li, Chunyan, Li, Daqing, Li, Deen, Li, Di, Li, Feng, Li, Guanyi, Li, Haiyang, Li, Hongwei, Li, Jia, Li, Jialin, Li, Jianan, Li, Jianguang, Li, Jiaying, Li, Jing, Li, Jing, Li, Jinmei, Li, Lala, Li, Li, Li, Lijun, Li, Liping, Li, Lize, Li, Mingju, Li, Minglan, Li, Mingyan, Li, Na, Li, Na, Li, Nan, Li, Nana, Li, Qiang, Li, Qianru, Li, Rui, Li, Ruihong, Li, Shanshan, Li, Shilin, Li, Si, Li, Suwen, Li, Tongshe, Li, Tongying, Li, Wanke, Li, Wei, Li, Wenbo, Li, Wenjuan, Li, Xi, Li, Xiangxia, Li, Xiao, Li, Xiaohui, Li, Xingyan, Li, Xiujuan, Li, Yan, Li, Yanfang, Li, Yang, Li, Yanxia, Li, Yaona, Li, Yichong, Li, Ying, Li, Yuqing, Li, Zheng, Li, Zhengye, Liang, Chuanliang, Liang, Jihua, Liang, Jin, Liang, Ke, Liang, Linju, Liang, Tingchen, Liang, Xia, Liang, Xianfeng, Liang, Yanli, Liang, Zhenye, Lie, Zhenbang, Lin, Qingfei, Lin, Ruifang, Lin, Xiao, Lin, Zhiqiang, Liu, Aijun, Liu, Chao, Liu, Chunxia, Liu, Cong, Liu, Fang, Liu, Fang, Liu, Guaiyan, Liu, Hongjun, Liu, Jiamin, Liu, Jiangling, Liu, Jianqi, Liu, Jieyun, Liu, Jihong, Liu, Jing, Liu, Jinsha, Liu, Juan, Liu, Junfang, Liu, Liming, Liu, Lin, Liu, Lin, Liu, Lin, Liu, Ling, Liu, Lu, Liu, Qiang, Liu, Qiaoling, Liu, Qiaoxia, Liu, Qiuxia, Liu, Shaobo, Liu, Xiaobao, Liu, Xiaocheng, Liu, Xiaoyuan, Liu, Xinbo, Liu, Xu, Liu, Yang, Liu, Yanhu, Liu, Yanming, Liu, Yaqin, Liu, Yong, Liu, Zhihong, Long, Jing, Lu, Futang, Lu, Huamei, Lu, Jiapeng, Lu, Junhong, Lu, Weibin, Lu, Yanrong, Lu, Yuchun, Luan, Tianwei, Luo, Qingwei, Luo, Qun, Luo, Tian, Luo, Xia, Luo, Yongmei, Lv, Jing, Lv, Jinhai, Lv, Lei, Lv, Lili, Lv, Meng, Ma, Aiqing, Ma, Huaimin, Ma, Huihuang, Ma, Jie, Ma, Jinbao, Ma, Li, Ma, Lingzhen, Ma, Nan, Ma, Qiaojuan, Ma, Shumei, Ma, Tengfei, Ma, Xiange, Ma, Xiaowen, Ma, Yuehua, Mai, Lanxian, Mei, Xiao, Meng, Gen, Miao, Ruichao, Miao, Xue, Miao, Xuyan, Min, Tingting, Mo, Shubing, Morigentu, Nan, Tingyan, Ni, Jinyang, Ni, Shuguo, Nie, Yu, Ning, Benxing, Ning, Xiaowei, Niu, Manman, Niu, Qingying, Niu, Wentang, Niu, Xiaoxia, Ou, Fang, Pan, Biyun, Pan, Chengjie, Pan, Congming, Pan, Jieli, Pan, Xiaowen, Pan, Ziying, Pei, Guangzhong, Pei, Lingyu, Pei, Min, Pei, Yanzhen, Peng, Yinyu, Peng, Yuming, Pu, Zhaokun, Qi, Fengjun, Qi, Liwei, Qi, Meiqiong, Qi, Yan, Qian, Jun, Qin, Lei, Qin, Zhonghua, Qing, Lan, Qiu, Lixia, Qiu, Weiyu, Qiu, Xiaoling, Qu, Yueli, Quan, Minghua, Ren, Dingping, Ren, Hong, Ren, Lingzhi, Ren, Tingting, Ren, Wei, Ren, Yihui, Rong, Yufang, Ruan, Jiahui, Shang, Peiqin, Shao, Minyan, Shao, Xuefeng, Shao, Yuling, Shen, Junrong, Shen, Rui, Sheng, Lin, Shi, Jiangjie, Shi, Xun, Shi, Yanhong, Shi, Yeju, Shi, Yujiao, Shu, Bo, Song, Bingchun, Song, Dan, Song, Jinhui, Song, Jinwang, Song, Jinxian, Song, Wei, Song, Xiaoping, Song, Yawen, Su, He, Su, Qinfeng, Su, Shuhong, Su, Xiaozhou, Sun, Chengxiang, Sun, Fangfang, Sun, Gongping, Sun, Jiangnan, Sun, Mengmeng, Sun, Rongrong, Sun, Shuting, Sun, Songtao, Sun, Ying, Sun, Yongmiao, Sun, Yunhong, Sun, Zhiqiang, Suo, Mengying, Tan, Binghu, Tang, Chunyan, Tang, Zhongli, Tao, Yu, Tian, Changming, Tian, Hongmei, Tian, Jian, Tian, Xiaomin, Wan, Huaibin, Wan, Qin, Wan, Rongjun, Wang, Bobin, Wang, Chao, Wang, Chaoqun, Wang, Chengliang, Wang, Di, Wang, Enfang, Wang, Feng, Wang, Gang, Wang, Guangqiang, Wang, Guixiang, Wang, Haifeng, Wang, Haijun, Wang, Haiyang, Wang, Jianfang, Wang, Jianfeng, Wang, Jing, Wang, Junping, Wang, Junying, Wang, Kang, Wang, Lei, Wang, Lei, Wang, Lin, Wang, Lize, Wang, Meng, Wang, Pan, Wang, Qi, Wang, Qiong, Wang, Qiuli, Wang, Qiuxue, Wang, Ran, Wang, Shaojin, Wang, Shuai, Wang, Tao, Wang, Tiantian, Wang, Tinghui, Wang, Tongyan, Wang, Wanhong, Wang, Wenjuan, Wang, Wenyan, Wang, Wenying, Wang, Wenzhuan, Wang, Xiaofei, Wang, Xiaoyan, Wang, Xitong, Wang, Xu, Wang, Yan, Wang, Yan, Wang, Yan, Wang, Yanfang, Wang, Yang, Wang, Yang, Wang, Yanping, Wang, Yanying, Wang, Yaoxin, Wang, Yingli, Wang, Yiting, Wang, Yue, Wang, Yumei, Wang, Yuzhuo, Wang, Zhenhua, Wang, Zhifang, Wang, Zhimin, Wei, Chunli, Wei, Lixia, Wei, Pei, Wei, Shuying, Wei, Xiqing, Wen, Hong, Wen, Yun, Wu, Chaoqun, Wu, Hairong, Wu, Lihua, Wu, Lingxiang, Wu, Qi, Wu, Shaorong, Wu, Wenting, Wu, Xueyi, Wu, Yongshuan, Wu, Zhihao, Wu, Zhuying, Wu, Zongyin, Wuhanbilige, Xia, Jun, Xia, Yang, Xiang, Jing, Xiao, Heliu, Xiao, Yaying, Xie, Meiling, Xie, Yinyan, Xin, Huiling, Xing, Jing, Xiu, Guoquan, Xu, Baohua, Xu, Chuangze, Xu, En, Xu, Jian, Xu, Shuli, Xu, Wei, Xu, Wen, Xue, Na, Xue, Tingting, Xue, Wei, Yan, Haiyan, Yan, Xiaofang, Yan, Yanqing, Yang, Bo, Yang, Hui, Yang, Huiyu, Yang, Jinhua, Yang, Kun, Yang, Man, Yang, Mengya, Yang, Ning, Yang, Ping, Yang, Xiajiao, Yang, Xiaomo, Yang, Xin, Yang, Xiujuan, Yang, Xuemei, Yang, Xuming, Yang, Yan, Yang, Yanhua, Yang, Yi, Yang, Yuanyuan, Yang, Zhimei, Yang, Zhiming, Yao, Hui, Yao, Lu, Ye, Jinling, Ye, Wenhua, Yi, Mingjiao, Yi, Shaowei, Yi, Wenyi, Yi, Zhimin, Yin, Guangxia, Yin, Guoyuan, Yu, Guibin, Yu, Hairong, Yu, Huaitao, Yu, Lijie, Yu, Lijun, Yu, Nana, Yu, Qin, Yu, Xinli, Yu, Yi, Yuan, Biao, Zeng, Chunmei, Zhai, Na, Zhai, Xiaojuan, Zhan, Hongju, Zhang, Aizhen, Zhang, Baohua, Zhang, Bin, Zhang, Caizhu, Zhang, Chaoying, Zhang, Chengbo, Zhang, Chunlai, Zhang, Churuo, Zhang, Fan, Zhang, Feiqin, Zhang, Ge, Zhang, Haibo, Zhang, Hailin, Zhang, Hanxue, Zhang, Huaixing, Zhang, Hui, Zhang, Huijuan, Zhang, Jinguo, Zhang, Jingyu, Zhang, Jinyun, Zhang, Jisheng, Zhang, Jun, Zhang, Lei, Zhang, Li, Zhang, Li, Zhang, Liang, Zhang, Lifeng, Zhang, Lina, Zhang, Liping, Zhang, Liping, Zhang, Min, Zhang, Ping, Zhang, Qiang, Zhang, Rufang, Zhang, Ruifen, Zhang, Shengde, Zhang, Siqi, Zhang, Sufang, Zhang, Tingting, Zhang, Wanyue, Zhang, Weiliang, Zhang, Xiaohan, Zhang, Xiaohong, Zhang, Xiaojuan, Zhang, Xin, Zhang, Xin, Zhang, Xue, Zhang, Xuewei, Zhang, Yachen, Zhang, Yang, Zhang, Yanyan, Zhang, Yaojie, Zhang, Yingyu, Zhang, Yuan, Zhang, Yun, Zhang, Yunfeng, Zhang, Zaozhang, Zhang, Zhichao, Zhao, Baihui, Zhao, Dan, Zhao, Fuxian, Zhao, Guizeng, Zhao, Haijie, Zhao, Honglei, Zhao, Huizhen, Zhao, Jindong, Zhao, Juan, Zhao, Liming, Zhao, Ling, Zhao, Lingshan, Zhao, Qingxia, Zhao, Qiuping, Zhao, Wanchen, Zhao, Wangxiu, Zhao, Weiyi, Zhao, Xiaodi, Zhao, Xiaojing, Zhao, Xiaoli, Zhao, Xiaoyan, Zhao, Xiling, Zhao, Yannan, Zhao, Yiyuan, Zheng, Shuzhen, Zheng, Xin, Zhi, Lixia, Zhong, Hui, Zhong, Qing, Zhong, Xin, Zhong, Yunzhi, Zhou, Jianfeng, Zhou, Jihu, Zhou, Ke, Zhou, Liangliang, Zhou, Ling, Zhou, Na, Zhou, Shengcheng, Zhou, Suyun, Zhou, Tao, Zhou, Wanren, Zhou, Weifeng, Zhou, Weijuan, Zhou, Xiaohong, Zhou, Yunke, Zhou, Yuquan, Zhou, Zhaohai, Zhou, Zhiming, Zhu, Bingpo, Zhu, Jifa, Zhu, Jing, Zhu, Mengnan, Zhu, Youcun, Zong, Dafei, Zuo, Hongbo, and Zuo, Zhaokai

Abstract

Uncertainty exists about whether lowering systolic blood pressure to less than 120 mm Hg is superior to that of less than 140 mm Hg, particularly in patients with diabetes and patients with previous stroke.

Published
2024
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27. Function of MYB8 in larch under PEG simulated drought stress.

Author

Zhao, Qingrong, Xiong, Huanhuan, Yu, Hongying, Wang, Chen, Zhang, Sufang, Hao, Junfei, Wang, Junhui, Zhang, Hanguo, and Zhang, Lei

Subjects
DROUGHT tolerance, AMINO acid metabolism, TRANSCRIPTION factors, GLUTAMIC acid, DROUGHTS, LARCHES, POLYETHYLENE glycol
Abstract

Larch, a prominent afforestation, and timber species in northeastern China, faces growth limitations due to drought. To further investigate the mechanism of larch's drought resistance, we conducted full-length sequencing on embryonic callus subjected to PEG-simulated drought stress. The sequencing results revealed that the differentially expressed genes (DEGs) primarily played roles in cellular activities and cell components, with molecular functions such as binding, catalytic activity, and transport activity. Furthermore, the DEGs showed significant enrichment in pathways related to protein processing, starch and sucrose metabolism, benzose-glucuronic acid interconversion, phenylpropyl biology, flavonoid biosynthesis, as well as nitrogen metabolism and alanine, aspartic acid, and glutamic acid metabolism. Consequently, the transcription factor T_transcript_77027, which is involved in multiple pathways, was selected as a candidate gene for subsequent drought stress resistance tests. Under PEG-simulated drought stress, the LoMYB8 gene was induced and showed significantly upregulated expression compared to the control. Physiological indices demonstrated an improved drought resistance in the transgenic plants. After 48 h of PEG stress, the transcriptome sequencing results of the transiently transformed LoMYB8 plants and control plants exhibited that genes were significantly enriched in biological process, cellular component and molecular function. Function analyses indicated for the enrichment of multiple KEGG pathways, including energy synthesis, metabolic pathways, antioxidant pathways, and other relevant processes. The pathways annotated by the differential metabolites mainly encompassed signal transduction, carbohydrate metabolism, amino acid metabolism, and flavonoid metabolism. [ABSTRACT FROM AUTHOR]

Published
2024
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28. Analysis of Fungal Diversity, Physicochemical Properties and Volatile Organic Compounds of Strong-Flavor Daqu from Seven Different Areas.

Author

Li, Zhigao, Yan, Xu, Zou, Sibo, Ji, Chaofan, Dong, Liang, Zhang, Sufang, Liang, Huipeng, and Lin, Xinping

Subjects
VOLATILE organic compounds, ETHYL esters, FLAVOR, MICROBIAL communities, PICHIA
Abstract

Strong-flavor Daqu, as a fermentation agent, plays a significant role in shaping the quality of strong-flavor baijius, and fungal species in Daqu are important factors affecting the quality of Daqu. Therefore, we selected strong-flavor Daqu from seven different origins to study the fungal composition and the effects of the fungal composition on the physicochemical properties and volatile organic compounds (VOCs). It was found that the fungal composition influences the physicochemical properties of Daqu. Specifically, there was a positive link between Rhizomucor, Rhizopus, Thermomyces, and liquefying activity and a positive correlation between Aspergillus and fermenting activity. Furthermore, the relationships between esterifying activity and Thermomyces, Rhizomucor, Aspergillus, Pichia, and Saccharomycopsis were found to be positive. The VOCs in Daqu were affected by Aspergillus, Issatchenkia, Pichia, and Thermoascus. Issatchenkia was significantly positively correlated with benzeneethanol as well as Aspergillus and pentadecanoic acid ethyl ester, ethyl myristate. Pichia and Thermoascus were significantly negatively correlated with benzaldehyde and 2-furaldehyde. This study deepens our understanding of the relationship between VOCs, the physicochemical properties with microbial communities, and reference significance for the production of better-quality strong-flavor Daqu. [ABSTRACT FROM AUTHOR]

Published
2024
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29. Masculinizer gene controls sexual differentiation in Hyphantria cunea.

Author

Li, Xiaowei, Liu, Huihui, Bi, Honglun, Wang, Yaohui, Xu, Jun, Zhang, Sufang, Zhang, Zhen, Zhang, Ze, and Huang, Yongping

Subjects
SEX differentiation (Embryology), SEX determination, ZINC-finger proteins, GENE expression, SILKWORMS
Abstract

The Masculinizer gene, Masc, encodes a lepidopteran‐specific novel CCCH‐type zinc finger protein, which controls sex determination and dosage compensation in Bombyx mori. Considering the potential application of it in pest control, it is necessary to investigate the function of Masc gene in Hyphantria cunea, a globally invasive forest pest. In the present study, we identified and functionally characterized the Masc gene, HcMasc, in H. cunea. Sequence analysis revealed that HcMASC contained the conserved CCCH‐type zinc finger domain, nuclear localization signal, and male determining domain, in which the last was confirmed to be required for its masculinization in BmN cell line. However, expression data showed that unlike male‐biased expression in B. mori, HcMasc gene expresses in main all developmental stages or tissues in both sexes. Clustered regularly interspaced palindromic repeats (CRISPR) / CRISPR‐associated protein 9‐based disruption of the common exons 1 and 3 of the HcMasc gene resulted in imbalanced sex ratio and abnormal external genitalia of both sexes. Our results suggest that the HcMasc gene is required for both male and female sexual differentiation and dosage compensation in H. cunea and provide a foundation for developing better strategies to control this pest. [ABSTRACT FROM AUTHOR]

Published
2024
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30. Effects of prebiotics on the fermentation of traditional suancai of Northeast China

Author

Zhao, Mingwei, Cao, Xinying, Wu, Yuzheng, Zou, Sibo, Li, Zhigao, Lin, Xinping, Ji, Chaofan, Dong, Liang, Zhang, Sufang, Yu, Chenxu, and Liang, Huipeng

Abstract

Suancai is a traditional fermented vegetable widely consumed in Northeast China. In this study, different prebiotics were used to improve the quality of suancai. Four prebiotics (inulin (INU), xylooligosaccharide (XOS), galactooligosaccharide (GOS), and stachyose (STA)) were shown to reduce the pH value and increase the content of total titratable acidity (TTA) in suancai, while the contents of most organic acids were also increased. The addition of prebiotics had significant effects on the bacterial microbiota during the suancai fermentation process. All prebiotics were shown to contribute to the growth of Lactobacillus. The suancai sample with fructooligosaccharides (FOS) had the highest relative abundance of Lactobacillus. Besides, INU and XOS could increase the abundance of Weissella. To evaluate the quality of suancai fermented with prebiotics, profiles of volatile flavor compounds (VOCs) and free amino acids (FAA) were analyzed. The prebiotics affected the VOCs and FAA profiles via transforming the bacterial microbiota. In addition, the addition of prebiotics also changed the taste profiles of the suancai samples. This study is among the first attempts to reveal the effects of different prebiotics on suancai fermentation, and the findings provide a foundation to develop new ways for improving the quality of suancai.

Published
2024
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31. Flavor profile and quality of strawberry wine are improved through sequential fermentation with indigenous non-Saccharomycesyeasts and Saccharomyces cerevisiae

Author

Yang, Bo, Liu, Shijiao, Zang, Hongwen, Dai, Yiwei, Zhang, Sufang, Lin, Xinping, Liang, Huipeng, and Chen, Yingxi

Abstract

In order to explore novel producing strategies for enhancing flavor compounds profile and quality of strawberry wine, we studied the effect of the sequential fermentation of two selected indigenous non-Saccharomycesyeasts (Hanseniaspora uvarumPGR9 and Kurtzmaniella quercitrusaPGT1) and Saccharomyces cerevisiaeCGMCC 2.3854 on the fermentation kinetics, physicochemical characteristics, organic acids, antioxidant activity, volatile compositions, and sensory properties. Results showed that the ethanol content produced by the fermentation of Kq-Scwas significantly lower than that of Scpure fermentation. In addition, the total volatile compounds concentration produced by the fermentation of Hu-Sc(16.2 ± 3.6 mg/L) and Kq-Sc(19.5 ± 3.1 mg/L) were significantly higher than that of Scpure fermentation (13.8 ± 2.0 mg/L). In particular, the Kq-Scstrawberry wine presented the highest number of relative odor-active compounds (rOAV ≥1). Moreover, strawberry wine fermented by Hu+Kq-Scshowed the highest ABTS free radical scavenging ability and the highest overall sensory qualities. The results indicated that the K. quercitrusaPGT1 strain could be an alternative yeast for producing strawberry wine, and the sequential fermentations of Kq-Sc and Hu+Kq-Scwere effective strategies for improving the flavor and quality of strawberry wine.

Published
2024
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32. Excellent iron-chelating capacity of Yesso scallop(Patinopecten yessoensis) skirt hydrolysate fermented by Bacillus subtilisM17-b7

Author

Yan, Xu, Yue, Ying, Pei, Qixuan, Zhang, Sufang, Ji, Chaofan, Chen, Yingxi, Dai, Yiwei, Dong, Liang, Zhu, Beiwei, and Lin, Xinping

Abstract

Peptide ferrous chelate is a new type of biological iron, which has the advantages of high absorption rate, safety and no digestive tract irritation. In this study, microbial fermentation was used for the first time to prepare iron-chelating peptides. Forty-eight strains were applied to ferment scallop skirts. A dominating strain was eliminated by using the iron binding capacity of the scallop skirt hydrolysate as the screening index. The scallop skirt hydrolysate fermented by Bacillus subtilisM17-b7 showed excellent iron-chelating capacity (96.20% ± 0.03%). Meanwhile, the structure, morphology and bioactivity of fermented scallop skirt hydrolysate (FSH) and fermented scallop skirt hydrolysate-iron (FSH–Fe) were measured. Based on the amino acid composition and spectral analysis, it was confirmed that the significant amino acids contains carboxyl, carbonyl, and amino groups. The result of scanning electron microscope indicated that the surface of FSH became porous and the particles were bigger after chelating with ferrous. Moreover, the peptide-ferrous chelate was more stable in salt solutions and simulated gastric juice compared with ferrous sulfate. It is also noteworthy that the preparation of ferrous chelate peptides by fermentation is cost-effective compared with enzymatic hydrolysis. Therefore, this study provides theoretical support for the use of fermentation as a promising tool to produce peptide-ferrous chelate.

Published
2024
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33. Effect of pectinase produced by Bacillus velezensisW17-6 on methanol content and overall quality of kiwifruit wine

Author

Wei, Xiaohan, Hao, Jie, Xiong, Kexin, Guo, Hui, Xue, Siyu, Dai, Yiwei, Zhang, Yujiao, Chen, Yingxi, and Zhang, Sufang

Abstract

Kiwifruit, a climacteric-fruit susceptible to rapid deterioration, attains considerable value upon transformation into kiwifruit wine, thereby increasing its added value. However, a noteworthy challenge arises from its high pectin content, which negatively affects byproduct quality, necessitating pectinase treatment during processing. Commercial pectinases exhibit heightened pectin esterase activity, giving rise to safety apprehensions due to augmented methanol content during kiwifruit pectin hydrolysis. In this study, Bacillus velezensisW17-6 was screened for its high pectinase activity coupled with low pectin esterase activity. The resultant crude pectinase named PBV was acquired through fed-batch-fermentation and partial purification. Subsequently, PBV was deployed in the fermentation of Xuxiang kiwifruit wine, and its impact on overall quality was assessed through diverse analyses. The results indicated that Xuxiang kiwifruit wine with PBV treatment outperformed counterparts treated with commercial pectinases. PBV treatment not only yielded superior clarity (83.75%±0.47%) and juice yield (81.60%±0.47%) but also increased the total flavonoids content in Xuxiang kiwifruit wine by 1.2 times compared to commercial pectinase. Notably, the methanol content of PBV-treated Xuxiang kiwifruit wine was significantly lower at 36.13±1.83 mg/L than that of wines treated with two commercial pectinases. PBV-treated kiwifruit wine also exhibited elevated concentrations of volatile compounds such as phenethyl alcohol, ethyl undecanoate, lauric aldehyde, isobutyric acid, and myrcene. Impressively, PBV-treated kiwifruit wine surpassed other wines in both electronic and artificial sensory analyses. In summary, PBV exhibits substantial potential in the production of low-methanol, high-quality kiwifruit wine, enhancing the flavor and nutritional value while minimizing methanol production and improving the safety profile.

Published
2024
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34. Disrupting the olfactory response in Monochamus saltuarius: Two potential target genes for controlling the vector insect of pine wilt disease.

Author

Zhang, Rong, Wang, Jue, Zhang, Yanlong, Wang, Xizhuo, Zhang, Zhen, Kong, Xiangbo, Liu, Fu, Fang, Jiaxing, Zheng, Yanan, and Zhang, Sufang

Subjects
*RNA interference, *GENE expression, *SMALL interfering RNA, *CHEMOSENSORY proteins, *GENE silencing, *OLFACTORY receptors, *CONIFER wilt
Abstract

Worldwide, pine forests have been threatened by a devastating pine wood disease caused by Bursaphelenchus xylophilus, with Monochamus saltuarius being a newly recorded vector of the disease in Northeast China. The olfactory system plays important roles in both feeding and oviposition during the adult stage of M. saltuarius, and olfactory gene function research is essential for gaining an understanding of the olfactory mechanisms of this pest. However, there is limited information available regarding olfactory gene functions in this pest. In the present study, we selected 7 olfactory genes, including 2 chemosensory proteins, 2 odorant‐binding proteins, the odorant co‐receptor and 2 odorant receptors, which were relatively highly expressed during the adult stage. We silenced these genes by RNA interference (RNAi), and real‐time quantitative PCR was used to detect their expression levels after double‐stranded RNA (dsRNA) injection. The results indicate that these genes were significantly downregulated at 2 d post‐dsRNA injection, and this was sustained until 5 d post‐injection. Electroantennography tests indicated that the knockdown of MsalOBP14 and MsalOrco impaired the olfactory response of M. saltuarius to 11 host volatiles and 1 sex pheromone compound. Y‐tube experiments further confirmed that downregulated MsalOBP14 and MsalOrco expression led to olfactory dysfunction in M. saltuarius, which significantly lost selectivity. The results indicate that MsalOBP14 and MsalOrco play critical roles in sex communication and host volatile detection in M. saltuarius, and possibly represent 2 effective targets for controlling this forest pest through olfactory disruption. [ABSTRACT FROM AUTHOR]

Published
2024
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35. Induction of antiherbivore defense responses in poplars using a methyl jasmonate and mesoporous silica nanoparticle complex.

Author

Fang J, Gan W, Wang Z, Zhang R, Zhang S, Liu F, Zhao X, and Kong X

Abstract

Background: Poplar in China has long been plagued by the fall webworm Hyphantria cunea. Enhancing plant immunity using chemical elicitors is an environmentally friendly approach to pest control. The phytohormone methyl jasmonate (MeJA) can stimulate the chemical defenses of poplars against herbivores but has been shown to have limited efficacy in practice. Here, we studied the effects of a MeJA and mesoporous silica nanoparticle (MSN) complex (MeJA@MSN) regarding the induction of poplar resistance to H. cunea, which may provide strategies for the effective use of MeJA., Results: The silicon-based phytohormone complex (MeJA@MSNs) exhibited excellent biological and physiochemical properties, such as excellent biocompatibility and plant tissue transportability. The changes in metabolites in poplar leaves induced by MeJA, MSNs, and MeJA@MSNs were investigated by metabolic analysis. MeJA@MSNs led to highly potent induced resistance along with elevated salicylaldehyde content, which increased with the dose administered. The salicylaldehyde metabolite showed a strong antifeedant effect on H. cunea larvae at a dosage of 1 μg, with the 50% lethal dose being 20.4 μg/mg. Furthermore, transcriptional analysis showed that MeJA@MSNs upregulated key genes in biosynthetic pathways more than MeJA and MSNs., Conclusion: Our results show that MeJA and MSNs interact positively in poplar, leading to salicylaldehyde accumulation and increased induced resistance to H. cunea, providing new insights into the underlying resistance mechanisms induced by MeJA@MSNs. © 2024 Society of Chemical Industry., (© 2024 Society of Chemical Industry.)

Published
2024
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36. Production optimization of food functional factor ergothioneine in wild-type red yeast Rhodotorula mucilaginosa DL-X01.

Author

Xiong K, Guo H, Xue S, Liu M, Dai Y, Lin X, and Zhang S

Subjects
Humans, Animals, Antioxidants metabolism, Histidine, Fermentation, Ergothioneine, Rhodotorula genetics, Rhodotorula metabolism, Monascus metabolism
Abstract

Background: Ergothioneine (EGT) is a high-value food functional factor that cannot be synthesized by humans and other vertebrates, and the low yield limits its application., Results: In this study, the optimal fermentation temperature, fermentation time, initial pH, inoculum age, and inoculation ratio on EGT biosynthesis of Rhodotorula mucilaginosa DL-X01 were optimized. In addition, the effects of three key precursor substances - histidine, methionine, and cysteine - on fungal EGT synthesis were verified. The optimal conditions were further obtained by response surface optimization. The EGT yield of R. mucilaginosa DL-X01 under optimal fermentation conditions reached 64.48 ± 2.30 mg L -1 at shake flask fermentation level. Finally, the yield was increased to 339.08 ± 3.31 mg L -1 (intracellular) by fed-batch fermentation in a 5 L bioreactor., Conclusion: To the best of our knowledge, this is the highest EGT yield ever reported in non-recombinant strains. The fermentation strategy described in this study will promote the efficient biosynthesis of EGT in red yeast and its sustainable production in the food industry. © 2024 Society of Chemical Industry., (© 2024 Society of Chemical Industry.)

Published
2024
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