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The hue‐recognition based point‐of‐care diagnosis favorites both human visual perception and imaging analysis with portable terminals. Semiconductor nanoplatelets (NPLs) as an emerging narrow‐emissive fluorophore particularly suit above color‐purity desired diagnosis, but are hindered by their intrinsic hydrophobicity and difficulty in controlled assembly. Here the host‐guest assembling of hydrophobic NPLs are developed within 3D porous space of dendritic templates, and stepwise surface silanization to fabricate silica‐NPLs‐silica structure (SNS). The structure enables high fluorescence of single assembly reaching 246‐fold and 4‐fold brightness of single NPL and commercial europium‐doped polystyrene bead. The strategy is compatible with various colored NPLs (≈540–640 nm emission) and provides stable aqueous colloids with well‐preserved emission. The high brightness and narrow emission of SNS enable both signal amplification and visual/image color discrimination. The hue‐recognizable lateral flow immunoassay for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS‐CoV‐2) nucleocapsid protein reveals an average naked‐eye interpretation accuracy of 92.9% over six concentration levels referring to standard card, accompanied by a quantification linear range of 0.13–200 ng mL−1 and a limit of detection of 0.13 ng mL−1 by smartphone. The diagnostic results for SARS‐CoV‐2 positive clinical samples demonstrate easily distinguishable antigen positive levels based on hue‐gradients, which holds significance for home‐based self‐testing of pathogens. [ABSTRACT FROM AUTHOR]

Stroke incidence is the most severe complication associated with atrial fibrillation (AF), and the most common site of thrombus formation in AF patients is the left atrial appendage (LAA). This study was developed to use two-dimensional speckle tracking imaging (2D-STI) to explore associations between LAA strain/strain rate and stroke incidence and to evaluate the value of utilizing LAA strain and strain rate values to support the stratification of nonvalvular AF (NVAF) patients based on stroke risk. A total of 486 AF patients who had undergone transesophageal echocardiography to exclude potential intracardiac thrombosis between March 2021 and November 2022 were consecutively enrolled. Patients meeting the inclusion criteria were separated into two groups according to their history of stroke/transient ischemic attack (TIA). LAA strain and strain rate values in these patients were measured via 2D-STI. Multivariable logistic regression analysis was employed to determine independent risk factors for the construction of a combined predictive model. Of the 333 analyzed patients (134 females, aged 65 (56,72) years), 39 (11.71%, 39/333) had a history of stroke at the time of evaluation. Multivariate logistic regression analysis demonstrated that nonparoxysmal AF, CHA2DS2VASc score, LAA thrombus/spontaneous echo contrast (SEC), LAA strain, and strain rate were all predictors of stroke incidence among NVAF patients. The combined predictive model demonstrated excellent discriminative ability, with an AUC of 0.91 (95%CI 0.87–0.95, P < 0.001), and a sensitivity and specificity of 79.49% and 89.46%, respectively. The Hosmer-Lemeshow test confirmed good calibration, yielding a value of 0.98. Comparative decision curve analysis showed that the model provided superior net benefits compared to the CHA2DS2VASc score. Furthermore, the model exhibited improved predictive performance and reclassification for stroke when compared to the CHA2DS2VASc score (AUC 0.91 vs. 0.88, Z = 2.32, P = 0.02), accompanied by a significant increase in the net reclassification index (+ 5.44%, P < 0.001) and integrated discrimination improvement (8.21%, P < 0.001). These data demonstrate that LAA strain and strain rate, as measured via 2D-STI, can offer value when assessing LAA function in AF patients, potentially providing further predictive value to extant clinical risk scoring strategies. [ABSTRACT FROM AUTHOR]