1. The upregulation and transcriptional regulatory mechanisms of Extra spindle pole bodies like 1 in bladder cancer: An immunohistochemistry and high-throughput screening Evaluation
- Author
-
Wei Zhang, Zi-Qian Liang, Rong-Quan He, Zhi-Guang Huang, Xiao-Min Wang, Mao-Yan Wei, Hui-Ling Su, Zhi-Su Liu, Yi-Sheng Zheng, Wan-Ying Huang, Han-Jie Zhang, Yi-Wu Dang, Sheng-Hua Li, Ji-Wen Cheng, Gang Chen, and Juan He
- Subjects
Gene expression ,Epigenetic regulation ,ESPL1 ,miR-299-5p ,Early screening ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Background: This study aimed to explore the expression level and transcriptional regulation mechanism of Extra Spindle Pole Bodies Like 1 (ESPL1) in bladder cancer (BC). Methods: A multicentre database of samples (n = 1391) was assayed for ESPL1 mRNA expression in BC and validated at the protein level by immunohistochemical (IHC) staining of in-house samples (n = 202). Single-cell sequencing (scRNA-seq) analysis and enrichment analysis explored ESPL1 distribution and their accompanying molecular mechanisms. ATAC-seq, ChIP-seq and Hi-C data from multiple platforms were used to investigate ESPL1 upstream transcription factors (TFs) and potential epigenetic regulatory mechanisms. Immune-related analysis, drug sensitivity and molecular docking of ESPL1 were also calculated. Furthermore, upstream microRNAs and the binding sites of ESPL1 were predicted. The expression level and early screening efficacy of miR-299-5p in blood (n = 6625) and tissues (n = 537) were examined. Results: ESPL1 was significantly overexpressed at the mRNA level (p
- Published
- 2024
- Full Text
- View/download PDF