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Abstract Key Message Leaves of seedlings from five oak species (Quercus robur L.; Q. pubescens L.; Q. suber L.; Q. afares Pomel; Q. ilex L.) displayed large, mainly inter-specific, differences in leaf mass-to-area ratio (LMA) and lignin content, as well as in the 13C composition of bulk leaf biomass. The variation in leaf lignin content and LMA did not impact the offset between the 13C composition measured in bulk leaf material versus soluble sugars. This observation, as well as the similar correlations between intrinsic leaf water use efficiency and the 13C compositions of bulk material or soluble sugars extracted from leaves, confirms their reliable use as a proxy for the former even when there is a large variation in LMA or lignin among samples. Context Carbon isotope composition (δ 13 C) of bulk leaf biomass is frequently used as a proxy for intrinsic water use efficiency (iWUE) in large-scale intra- and inter-specific comparisons. However, post-photosynthetic 13C discrimination during the synthesis of lignin combined with differences in leaf mass-to-area ratio (LMA) may bias the relationship between δ 13 C of bulk leaf matter and iWUE and thus its use as a proxy of iWUE. Aims To quantify the impact of differences in lignin content and LMA on the relationship between δ 13 C of bulk leaf biomass and iWUE over a large gradient of lignin contents across five oak species (deciduous: Quercus robur, Q. pubescens, Q. afares and evergreen: Q. ilex and Q. suber). Methods We measured lignin content, LMA, and δ 13 C of bulk leaf biomass and of soluble sugars extracted from the leaves, as well as intrinsic water use efficiency (derived from leaf gas exchange) in seedlings of the five oak species grown under common conditions in a greenhouse and measured in a climate chamber. Results There was a large range (mainly across species) in lignin content (4 to 33%) and LMA (60–180 g m−2). δ 13 C of bulk leaf biomass and soluble sugars were tightly correlated, showing a significant mean offset of − 0.4‰. This offset was stable across species and not correlated to the lignin content of the leaves. A very loose correlation was found between the offset and LMA, mainly due to one species. Conclusion Our results are a demonstration that potential variations in leaf lignin content or LMA have no or only a little effect on the δ 13 C of bulk leaf biomass. They are unlikely to cause a bias when using bulk leaf δ 13 C as a proxy for variations in intrinsic water use efficiency among Mediterranean and temperate broad-leaf forest tree species.

Key Message: Leaves of seedlings from five oak species (Quercus robur L.; Q. pubescens L.; Q. suber L.; Q. afares Pomel; Q. ilex L.) displayed large, mainly inter-specific, differences in leaf mass-to-area ratio (LMA) and lignin content, as well as in the 13C composition of bulk leaf biomass. The variation in leaf lignin content and LMA did not impact the offset between the 13C composition measured in bulk leaf material versus soluble sugars. This observation, as well as the similar correlations between intrinsic leaf water use efficiency and the 13C compositions of bulk material or soluble sugars extracted from leaves, confirms their reliable use as a proxy for the former even when there is a large variation in LMA or lignin among samples. Context: Carbon isotope composition (δ13C) of bulk leaf biomass is frequently used as a proxy for intrinsic water use efficiency (iWUE) in large-scale intra- and inter-specific comparisons. However, post-photosynthetic 13C discrimination during the synthesis of lignin combined with differences in leaf mass-to-area ratio (LMA) may bias the relationship between δ13C of bulk leaf matter and iWUE and thus its use as a proxy of iWUE. Aims: To quantify the impact of differences in lignin content and LMA on the relationship between δ13C of bulk leaf biomass and iWUE over a large gradient of lignin contents across five oak species (deciduous: Quercus robur, Q. pubescens, Q. afares and evergreen: Q. ilex and Q. suber). Methods: We measured lignin content, LMA, and δ13C of bulk leaf biomass and of soluble sugars extracted from the leaves, as well as intrinsic water use efficiency (derived from leaf gas exchange) in seedlings of the five oak species grown under common conditions in a greenhouse and measured in a climate chamber. Results: There was a large range (mainly across species) in lignin content (4 to 33%) and LMA (60–180 g m−2). δ13C of bulk leaf biomass and soluble sugars were tightly correlated, showing a significant mean offset of − 0.4‰. This offset was stable across species and not correlated to the lignin content of the leaves. A very loose correlation was found between the offset and LMA, mainly due to one species. Conclusion: Our results are a demonstration that potential variations in leaf lignin content or LMA have no or only a little effect on the δ13C of bulk leaf biomass. They are unlikely to cause a bias when using bulk leaf δ13C as a proxy for variations in intrinsic water use efficiency among Mediterranean and temperate broad-leaf forest tree species. [ABSTRACT FROM AUTHOR]

The natural gas resources in the Sichuan Basin are abundant and exhibit various phenomena, for example, mixed sources, mixed maturities and overlapping combinations, resulting in great difficulties in identifying natural gas sources. In this study, several groups of thermal simulation experiments were conducted on various types of kerogens using closed and/or semi‐open systems to investigate the characteristics of the carbon isotope compositions of light hydrocarbon gases derived from different typical kerogens. The results showed that the carbon isotope compositions and variation trends of methane and ethane are controlled by the kerogen type, thermal simulation system and thermal evolution degree. The carbon isotope compositions of light hydrocarbon gases derived from different types of kerogens exhibit some differences with increased thermal evolution degree. When the thermal simulation temperature increases from 320 to 550°C, the methane carbon isotope of typically marine source rocks, mainly type I and type II1 kerogens, is slightly higher than or equivalent to that of ethane. However, the ethane carbon isotope of terrestrial source rocks, primarily types II2 and III kerogens, is significantly heavier than methane. By comparison, the evolution trend of carbon isotope compositions of actual natural gas is well consistent with that of light hydrocarbon gases cracked from thermal simulation experiments with increased thermal evolution degree. These findings are of great importance to identify the source rock of actual natural gas with high thermal maturities, as well as to determine the sources of deep shale gas in the southern Sichuan Basin, the Yuanba gas field and the Puguang gas field. Finally, a fitting method of carbon isotope values of methane and ethane of light hydrocarbon gases can be used to more finely differentiate the sources of natural gas in complex areas with mixed sources, mixed maturities and overlapping combinations. [ABSTRACT FROM AUTHOR]

Straw application is an effective and common measure to improve soil quality but generally increases greenhouse gas (GHG) emissions. The combined application of biochar and straw has been proposed to mitigate these emissions. However, the effects of the combined application of biochar and maize straw, particularly at different proportions, on GHG emissions remain inadequately understood. In this study, an incubation experiment was conducted with five treatments: soil only (CK), 1 % maize straw application (TS), 0.7 % maize straw +0.3 % biochar application (S7B3), 0.5 % maize straw +0.5 % biochar application (S5B5) and 0.3 % maize straw +0.7 % biochar application (S3B7). The study also considered the effect of 1 % biochar application (TB) from our previous study with the same soil type and incubation condition. Carbon isotope technology was utilized to trace CO 2 sources and assess the priming effects on soil organic carbon (SOC) mineralization. The results showed that TB reduced CO 2 emission, while TS increased CO 2 emission due to maize straw decomposition and its positive priming effect on native SOC mineralization. S7B3, S5B5 and S3B7 also increased CO 2 emissions, but with significantly lower emissions than TS, in the order of TS > S7B3 > S5B5 ≥ S3B7. The positive priming effects of S7B3, S5B5 and S3B7 on native SOC mineralization weakened over time and eventually turned negative. TB, TS and combined applications reduced N 2 O emission due to decreased substrates (NH 4 + and NO 3 −) for nitrification and denitrification, induced by promoting microbial fixation of inorganic nitrogen. The reduction effects on N 2 O emission of combined applications were superior to those of TS and TB, with S5B5 demonstrating the best efficacy. TS increased CH 4 emission, while S7B3, S5B5 and S3B7 reduced CH 4 emission. The reduction effects of CH 4 emission with combined applications were superior to that of TB, and S5B5 exhibited the lowest CH 4 emission. Unlike soils with higher nitrogen content, where N 2 O emission dominated the global warming potential (GWP), CO 2 emission dominated the GWP in this study, resulting in increased GWP in TS, S7B3, S5B5 and S3B7, but with significantly lower values for S7B3, S5B5 and S3B7 compared to TS. In conclusion, our study suggests that the combined application of biochar and maize straw, especially S5B5, could effectively mitigate GHG emissions promoted by maize straw application, especially in soils with higher nitrogen content. [Display omitted] • Biochar and maize straw co-application reduced CO 2 emission promoted by maize straw application. • The priming effect of co-application on SOC shifted from positive to negative over time. • Co-application reduced stimulation of CH 4 emission caused by maize straw application. • Co-application exhibited superior efficacy in reducing N 2 O emissions. • The optimal co-application ratio for mitigating greenhouse gas emissions was 1:1. [ABSTRACT FROM AUTHOR]

Intra-annual variations of carbon stable isotope ratios (δ13C) in different tree compartments could represent valuable indicators of plant carbon source-sink dynamics, at weekly time scale. Despite this significance, the absence of a methodological framework for tracking δ13C values in tree rings persists due to the complexity of tree ring development. To fulfill this knowledge gap, we developed a method to monitor weekly variability of δ13C in the cambium-xylem continuum of black spruce species [Picea mariana (Mill.) BSP.] during the growing season. We collected and isolated the weekly incremental growth of the cambial region and the developing tree ring from five mature spruce trees over three consecutive growing seasons (2019-2021) in Simoncouche and two growing seasons (2020-2021) in Bernatchez, both located in the boreal forest of Quebec, Canada. Our method allowed for the creation of intra-annual δ13C series for both the growing cambium (δ13Ccam) and developing xylem cellulose (δ13Cxc) in these two sites. Strong positive correlations were observed between δ13Ccam and δ13Cxc series in almost all study years. These findings suggest that a constant supply of fresh assimilates to the cambium-xylem continuum may be the dominant process feeding secondary growth in the two study sites. On the other hand, rates of carbon isotopic fractionation appeared to be poorly affected by climate variability, at an inter- weekly time scale. Hence, increasing δ13Ccam and δ13Cxc trends highlighted here possibly indicate shifts in carbon allocation strategies, likely fostering frost resistance and reducing water uptake in the late growth season. Additionally, these trends may be related to the black spruce trees' responses to the seasonal decrease in photosynthetically active radiation. Our findings provide new insights into the seasonal carbon dynamics and growth constraints of black spruce in boreal forest ecosystems, offering a novel methodological approach for studying carbon allocation at fine temporal scales., (© The Author(s) 2024. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

The ratio of net CO2 uptake (Anet) and stomatal conductance (gs) is an intrinsic measurement of leaf water use efficiency (WUEi) however its measurement can be challenging for large phenotypic screens. Measurements of leaf carbon isotope composition (δ13Cleaf) may be a scalable tool to approximate WUEi for screening because it in part reflects the competing influences of Anet and gs on the CO2 partial pressure (pCO2) inside the leaf over time. However, in C4 photosynthesis the CO2 concentrating mechanism complicates the relationship between δ13Cleaf and WUEi. Despite this complicated relationship, several studies have shown genetic variation in δ13Cleaf across C4 plants. Yet there has not been a clear demonstration of whether Anet or gs are the causal mechanisms controlling WUEi and δ13Cleaf. Our approach was to characterize leaf photosynthetic traits of two Zea mays recombinant inbred lines (Z007E0067 and Z007E0150) which consistently differ for δ13Cleaf even though they have minimal confounding genetic differences. We demonstrate that these two genotypes contrasted in WUEi driven by differences in the speed of stomatal responses to changes in pCO2 and light that lead to unproductive leaf water loss. These findings provide support that differences in δ13Cleaf in closely related genotypes do reflect greater WUEi and further suggests that differences in stomatal kinetic response to changing environmental conditions is a key target to improve WUEi., (© The Author(s) 2024. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.)